##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1525951_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1351 Sequences flagged as poor quality 0 Sequence length 40 %GC 54 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.80162842339008 32.0 26.0 35.0 4.0 37.0 2 20.12879348630644 23.0 4.0 32.0 4.0 36.0 3 18.974833456698743 22.0 4.0 31.0 4.0 35.0 4 18.552923760177645 19.0 4.0 32.0 4.0 35.0 5 16.585492227979273 10.0 4.0 31.0 4.0 36.0 6 14.082901554404145 4.0 4.0 27.0 4.0 33.0 7 13.84900074019245 4.0 4.0 27.0 4.0 33.0 8 13.485566247224279 4.0 4.0 26.0 4.0 33.0 9 13.179866765358993 4.0 4.0 26.0 4.0 33.0 10 12.344929681717247 4.0 4.0 24.0 4.0 33.0 11 9.985936343449296 4.0 4.0 13.0 4.0 30.0 12 9.809030347890452 4.0 4.0 11.0 4.0 30.0 13 9.302738712065137 4.0 4.0 10.0 4.0 28.0 14 9.527757216876388 4.0 4.0 7.0 4.0 30.0 15 9.082901554404145 4.0 4.0 4.0 4.0 30.0 16 7.74019245003701 4.0 4.0 4.0 4.0 24.0 17 7.250925240562546 4.0 4.0 4.0 4.0 20.0 18 7.227979274611399 4.0 4.0 4.0 4.0 22.0 19 6.852701702442635 4.0 4.0 4.0 4.0 18.0 20 6.954848260547743 4.0 4.0 4.0 4.0 19.0 21 6.660251665433012 4.0 4.0 4.0 4.0 17.0 22 6.713545521835678 4.0 4.0 4.0 4.0 18.0 23 6.593634344929682 4.0 4.0 4.0 4.0 17.0 24 6.564766839378239 4.0 4.0 4.0 4.0 17.0 25 6.647668393782383 4.0 4.0 4.0 4.0 18.0 26 6.65358993338268 4.0 4.0 4.0 4.0 17.0 27 6.750555144337528 4.0 4.0 4.0 4.0 19.0 28 6.735011102886751 4.0 4.0 4.0 4.0 18.0 29 6.593634344929682 4.0 4.0 4.0 4.0 16.0 30 6.613619541080681 4.0 4.0 4.0 4.0 17.0 31 7.059955588452998 4.0 4.0 4.0 4.0 22.0 32 6.947446336047372 4.0 4.0 4.0 4.0 19.0 33 7.020725388601036 4.0 4.0 4.0 4.0 21.0 34 7.015544041450777 4.0 4.0 4.0 4.0 22.0 35 6.8630643967431535 4.0 4.0 4.0 4.0 19.0 36 7.0 4.0 4.0 4.0 4.0 23.0 37 6.794966691339749 4.0 4.0 4.0 4.0 17.0 38 6.745373797187269 4.0 4.0 4.0 4.0 15.0 39 6.6654330125832715 4.0 4.0 4.0 4.0 16.0 40 6.70540340488527 4.0 4.0 4.0 4.0 15.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2 1 -6.01556776556777 2 2 -0.7271062271062263 2 3 2.166666666666668 2 4 2.739010989010989 2 5 5.100732600732602 2 6 -10.339743589743588 2 7 -9.911172161172159 2 8 -9.966117216117214 2 9 -9.017399267399268 2 10 -8.576007326007327 2 11 -6.3598901098901095 2 12 -5.93956043956044 2 13 -5.7866300366300365 2 14 -5.589743589743589 2 15 -5.542124542124542 2 16 -3.613553113553114 2 17 -3.2142857142857144 2 18 -3.4615384615384617 2 19 -2.6391941391941387 2 20 -2.8663003663003668 2 21 -1.9249084249084243 2 22 -1.9835164835164836 2 23 -1.9230769230769234 2 24 -1.9963369963369964 2 25 -1.655677655677657 2 26 -1.75091575091575 2 27 -1.8186813186813184 2 28 -1.6739926739926743 2 29 -1.8589743589743586 2 30 -1.4285714285714288 2 31 -1.9542124542124544 2 32 -1.4267399267399261 2 33 -1.6941391941391952 2 34 -1.824175824175824 2 35 -1.9358974358974352 2 36 -1.8076923076923075 2 37 -2.042124542124543 2 38 -1.26007326007326 2 39 -1.644688644688646 2 40 -1.4981684981684982 3 1 5.98443223443223 3 2 5.772893772893774 3 3 3.666666666666668 3 4 2.239010989010989 3 5 1.6007326007326022 3 6 8.160256410256412 3 7 13.588827838827841 3 8 16.533882783882788 3 9 13.982600732600732 3 10 13.423992673992673 3 11 16.64010989010989 3 12 7.56043956043956 3 13 5.2133699633699635 3 14 -4.589743589743589 3 15 3.457875457875458 3 16 9.886446886446887 3 17 10.285714285714285 3 18 10.038461538461538 3 19 1.3608058608058613 3 20 10.133699633699633 3 21 12.575091575091577 3 22 9.516483516483516 3 23 10.576923076923077 3 24 9.503663003663004 3 25 9.344322344322343 3 26 2.74908424908425 3 27 7.681318681318682 3 28 11.826007326007325 3 29 10.141025641025642 3 30 9.071428571428571 3 31 9.045787545787546 3 32 12.073260073260073 3 33 9.305860805860805 3 34 9.675824175824175 3 35 7.064102564102565 3 36 9.692307692307693 3 37 10.457875457875456 3 38 12.73992673992674 3 39 10.355311355311354 3 40 12.501831501831502 5 1 5.23443223443223 5 2 -8.227106227106226 5 3 -8.833333333333332 5 4 -13.010989010989011 5 5 -10.399267399267398 5 6 -3.5897435897435876 5 7 -8.161172161172159 5 8 -8.716117216117214 5 9 -4.267399267399268 5 10 -1.8260073260073266 5 11 -0.6098901098901095 5 12 -4.43956043956044 5 13 -2.5366300366300365 5 14 1.4102564102564106 5 15 1.957875457875458 5 16 -3.613553113553114 5 17 -3.2142857142857144 5 18 -3.4615384615384617 5 19 -2.6391941391941387 5 20 -2.8663003663003668 5 21 -1.9249084249084243 5 22 -1.9835164835164836 5 23 -1.9230769230769234 5 24 -1.9963369963369964 5 25 -1.655677655677657 5 26 -1.75091575091575 5 27 -1.8186813186813184 5 28 -1.6739926739926743 5 29 -1.8589743589743586 5 30 -1.4285714285714288 5 31 -1.9542124542124544 5 32 -1.4267399267399261 5 33 -1.6941391941391952 5 34 -1.824175824175824 5 35 -1.9358974358974352 5 36 -1.8076923076923075 5 37 -2.042124542124543 5 38 -1.26007326007326 5 39 -1.644688644688646 5 40 -1.4981684981684982 6 1 -23.01556776556777 6 2 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15.166666666666668 7 4 17.73901098901099 7 5 20.100732600732602 7 6 -2.3397435897435876 7 7 15.088827838827841 7 8 19.033882783882788 7 9 16.98260073260073 7 10 20.42399267399267 7 11 15.64010989010989 7 12 20.060439560439562 7 13 22.213369963369964 7 14 13.41025641025641 7 15 23.45787545787546 7 16 10.386446886446887 7 17 9.785714285714285 7 18 20.53846153846154 7 19 19.36080586080586 7 20 14.133699633699633 7 21 25.075091575091577 7 22 24.016483516483518 7 23 21.076923076923077 7 24 22.003663003663004 7 25 9.344322344322343 7 26 20.249084249084248 7 27 14.181318681318682 7 28 12.326007326007325 7 29 10.141025641025642 7 30 4.571428571428571 7 31 25.045787545787547 7 32 15.573260073260073 7 33 15.305860805860805 7 34 27.175824175824175 7 35 22.064102564102566 7 36 17.192307692307693 7 37 22.957875457875456 7 38 13.73992673992674 7 39 18.355311355311354 7 40 20.501831501831504 8 1 5.98443223443223 8 2 -3.2271062271062263 8 3 8.166666666666668 8 4 7.739010989010989 8 5 17.100732600732602 8 6 11.660256410256412 8 7 19.08882783882784 8 8 16.033882783882788 8 9 14.982600732600732 8 10 20.42399267399267 8 11 -6.3598901098901095 8 12 -5.93956043956044 8 13 -5.7866300366300365 8 14 -5.589743589743589 8 15 -5.542124542124542 8 16 -3.613553113553114 8 17 -3.2142857142857144 8 18 -3.4615384615384617 8 19 -2.6391941391941387 8 20 -2.8663003663003668 8 21 -1.9249084249084243 8 22 -1.9835164835164836 8 23 -1.9230769230769234 8 24 -1.9963369963369964 8 25 -1.655677655677657 8 26 -1.75091575091575 8 27 -1.8186813186813184 8 28 -1.6739926739926743 8 29 -1.8589743589743586 8 30 -1.4285714285714288 8 31 -1.9542124542124544 8 32 -1.4267399267399261 8 33 -1.6941391941391952 8 34 -1.824175824175824 8 35 -1.9358974358974352 8 36 -1.8076923076923075 8 37 -2.042124542124543 8 38 -1.26007326007326 8 39 -1.644688644688646 8 40 -1.4981684981684982 9 1 2.98443223443223 9 2 14.272893772893774 9 3 10.666666666666668 9 4 11.739010989010989 9 5 0.10073260073260215 9 6 -3.3397435897435876 9 7 -2.911172161172159 9 8 -2.9661172161172136 9 9 -2.0173992673992682 9 10 -1.5760073260073266 9 11 -0.8598901098901095 9 12 -0.4395604395604398 9 13 10.713369963369964 9 14 0.9102564102564106 9 15 3.457875457875458 9 16 6.886446886446886 9 17 2.7857142857142856 9 18 12.038461538461538 9 19 2.8608058608058613 9 20 10.633699633699633 9 21 13.075091575091577 9 22 5.016483516483516 9 23 6.076923076923077 9 24 6.003663003663004 9 25 6.344322344322343 9 26 3.74908424908425 9 27 3.1813186813186816 9 28 11.326007326007325 9 29 13.641025641025642 9 30 5.071428571428571 9 31 1.0457875457875456 9 32 -1.4267399267399261 9 33 5.305860805860805 9 34 10.175824175824175 9 35 11.564102564102566 9 36 2.6923076923076925 9 37 11.957875457875456 9 38 1.73992673992674 9 39 12.355311355311354 9 40 10.501831501831502 10 1 5.98443223443223 10 2 -7.227106227106226 10 3 14.166666666666668 10 4 14.739010989010989 10 5 -11.899267399267398 10 6 -10.339743589743588 10 7 -9.911172161172159 10 8 -9.966117216117214 10 9 -9.017399267399268 10 10 -8.576007326007327 10 11 -6.3598901098901095 10 12 -5.93956043956044 10 13 -5.7866300366300365 10 14 -5.589743589743589 10 15 -5.542124542124542 10 16 -3.613553113553114 10 17 -3.2142857142857144 10 18 -3.4615384615384617 10 19 -2.6391941391941387 10 20 -2.8663003663003668 10 21 -1.9249084249084243 10 22 -1.9835164835164836 10 23 -1.9230769230769234 10 24 -1.9963369963369964 10 25 -1.655677655677657 10 26 -1.75091575091575 10 27 -1.8186813186813184 10 28 -1.6739926739926743 10 29 -1.8589743589743586 10 30 -1.4285714285714288 10 31 -1.9542124542124544 10 32 -1.4267399267399261 10 33 -1.6941391941391952 10 34 -1.824175824175824 10 35 -1.9358974358974352 10 36 -1.8076923076923075 10 37 -2.042124542124543 10 38 -1.26007326007326 10 39 -1.644688644688646 10 40 -1.4981684981684982 11 1 3.98443223443223 11 2 -15.227106227106226 11 3 -14.833333333333332 11 4 -14.260989010989011 11 5 -11.899267399267398 11 6 -10.339743589743588 11 7 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15 5 5.100732600732602 15 6 7.660256410256412 15 7 13.088827838827841 15 8 15.033882783882786 15 9 3.9826007326007318 15 10 12.423992673992673 15 11 24.64010989010989 15 12 23.060439560439562 15 13 7.2133699633699635 15 14 20.41025641025641 15 15 21.45787545787546 15 16 -3.613553113553114 15 17 -3.2142857142857144 15 18 -3.4615384615384617 15 19 -2.6391941391941387 15 20 -2.8663003663003668 15 21 -1.9249084249084243 15 22 -1.9835164835164836 15 23 -1.9230769230769234 15 24 -1.9963369963369964 15 25 -1.655677655677657 15 26 -1.75091575091575 15 27 -1.8186813186813184 15 28 -1.6739926739926743 15 29 -1.8589743589743586 15 30 -1.4285714285714288 15 31 -1.9542124542124544 15 32 -1.4267399267399261 15 33 -1.6941391941391952 15 34 -1.824175824175824 15 35 -1.9358974358974352 15 36 -1.8076923076923075 15 37 -2.042124542124543 15 38 -1.26007326007326 15 39 -1.644688644688646 15 40 -1.4981684981684982 16 1 3.98443223443223 16 2 10.772893772893774 16 3 0.16666666666666785 16 4 -2.760989010989011 16 5 7.100732600732602 16 6 18.160256410256412 16 7 2.088827838827841 16 8 5.033882783882786 16 9 -0.017399267399268226 16 10 0.4239926739926734 16 11 15.64010989010989 16 12 10.56043956043956 16 13 14.213369963369964 16 14 0.9102564102564106 16 15 4.957875457875458 16 16 1.386446886446886 16 17 1.7857142857142856 16 18 9.538461538461538 16 19 1.3608058608058613 16 20 11.633699633699633 16 21 9.075091575091577 16 22 9.016483516483516 16 23 11.576923076923077 16 24 8.003663003663004 16 25 2.844322344322343 16 26 7.24908424908425 16 27 4.681318681318682 16 28 10.826007326007325 16 29 11.641025641025642 16 30 9.571428571428571 16 31 12.545787545787546 16 32 3.573260073260074 16 33 3.3058608058608048 16 34 12.675824175824175 16 35 12.564102564102566 16 36 2.1923076923076925 16 37 12.457875457875456 16 38 -1.26007326007326 16 39 -1.644688644688646 16 40 -1.4981684981684982 17 1 5.48443223443223 17 2 3.2728937728937737 17 3 7.666666666666668 17 4 9.739010989010989 17 5 14.600732600732602 17 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-1.8589743589743586 119 30 -1.4285714285714288 119 31 -1.9542124542124544 119 32 -1.4267399267399261 119 33 -1.6941391941391952 119 34 -1.824175824175824 119 35 -1.9358974358974352 119 36 -1.8076923076923075 119 37 -2.042124542124543 119 38 -1.26007326007326 119 39 -1.644688644688646 119 40 -1.4981684981684982 120 1 -23.01556776556777 120 2 -15.227106227106226 120 3 -14.833333333333332 120 4 -14.260989010989011 120 5 -11.899267399267398 120 6 -10.339743589743588 120 7 -9.911172161172159 120 8 -9.966117216117214 120 9 -9.017399267399268 120 10 -8.576007326007327 120 11 -6.3598901098901095 120 12 -5.93956043956044 120 13 -5.7866300366300365 120 14 -5.589743589743589 120 15 -5.542124542124542 120 16 -3.613553113553114 120 17 -3.2142857142857144 120 18 -3.4615384615384617 120 19 -2.6391941391941387 120 20 -2.8663003663003668 120 21 -1.9249084249084243 120 22 -1.9835164835164836 120 23 -1.9230769230769234 120 24 -1.9963369963369964 120 25 -1.655677655677657 120 26 -1.75091575091575 120 27 -1.8186813186813184 120 28 -1.6739926739926743 120 29 -1.8589743589743586 120 30 -1.4285714285714288 120 31 -1.9542124542124544 120 32 -1.4267399267399261 120 33 -1.6941391941391952 120 34 -1.824175824175824 120 35 -1.9358974358974352 120 36 -1.8076923076923075 120 37 -2.042124542124543 120 38 -1.26007326007326 120 39 -1.644688644688646 120 40 -1.4981684981684982 >>END_MODULE >>Per sequence quality scores fail #Quality Count 4 432.0 5 109.0 6 86.0 7 121.0 8 85.0 9 69.0 10 70.0 11 58.0 12 38.0 13 33.0 14 18.0 15 16.0 16 14.0 17 11.0 18 9.0 19 5.0 20 14.0 21 23.0 22 29.0 23 31.0 24 25.0 25 14.0 26 12.0 27 9.0 28 11.0 29 5.0 30 2.0 31 1.0 32 0.0 33 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 0.0 0.0 100.0 0.0 3 0.0 0.0 0.0 100.0 4 0.0 0.0 0.0 100.0 5 0.0 0.0 0.0 100.0 6 100.0 0.0 0.0 0.0 7 98.88888888888889 0.5185185185185185 0.14814814814814814 0.4444444444444444 8 97.62962962962963 0.962962962962963 0.7407407407407408 0.6666666666666667 9 95.70051890289103 2.001482579688658 1.1119347664936992 1.1860637509266123 10 54.525222551928785 22.997032640949556 8.753709198813056 13.724035608308604 11 35.751295336787564 25.314581791265727 21.317542561065878 17.616580310880828 12 37.67579570688379 20.429311621021466 21.68763878608438 20.207253886010363 13 24.51851851851852 19.333333333333332 26.740740740740744 29.40740740740741 14 20.059215396002962 29.163582531458175 30.495928941524795 20.281273131014064 15 30.421909696521094 25.166543301258326 29.23760177646188 15.173945225758697 16 29.311621021465584 29.681717246484084 28.275351591413767 12.731310140636564 17 29.829755736491485 26.86898593634345 28.645447816432274 14.655810510732792 18 30.199851961509992 27.905255366395266 23.83419689119171 18.060695780903036 19 22.74074074074074 31.25925925925926 26.074074074074073 19.925925925925924 20 27.387120651369358 29.163582531458175 28.497409326424872 14.951887490747595 21 23.908216136195414 27.979274611398964 28.867505551443372 19.24500370096225 22 21.46558105107328 27.091043671354555 31.53219837157661 19.91117690599556 23 22.057735011102885 30.6439674315322 31.902294596595116 15.3960029607698 24 20.873427091043673 30.05181347150259 27.461139896373055 21.61361954108068 25 20.22222222222222 30.592592592592595 25.77777777777778 23.40740740740741 26 27.40740740740741 31.703703703703706 23.77777777777778 17.11111111111111 27 20.11878247958426 29.621380846325167 23.385300668151448 26.874536005939127 28 18.592592592592595 33.25925925925926 24.666666666666668 23.48148148148148 29 26.464047442550036 29.65159377316531 24.53669384729429 19.347664936990363 30 23.390081421169505 30.940044411547003 27.757216876387865 17.912657290895634 31 27.387120651369358 28.71946706143597 27.01702442635085 16.87638786084382 32 28.847583643122675 27.434944237918213 27.806691449814124 15.91078066914498 33 27.831236121391562 28.71946706143597 21.909696521095483 21.53960029607698 34 20.947446336047374 28.34937083641747 32.93856402664693 17.764618800888233 35 18.726868985936342 34.196891191709845 30.12583271650629 16.95040710584752 36 19.49592290585619 34.766493699036324 26.612305411415864 19.125277983691625 37 22.35381199111769 30.569948186528496 28.941524796447077 18.134715025906736 38 21.095484826054776 34.34492968171725 25.018504811250924 19.541080680977053 39 25.536639526276833 30.495928941524795 23.316062176165804 20.651369356032568 40 20.355292376017765 28.645447816432274 27.461139896373055 23.538119911176906 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 1.0 14 0.5 15 0.0 16 1.5 17 3.0 18 3.0 19 5.5 20 8.0 21 7.5 22 7.0 23 7.0 24 8.0 25 9.0 26 9.5 27 10.0 28 10.0 29 9.5 30 9.0 31 7.5 32 6.0 33 6.0 34 15.0 35 24.0 36 27.5 37 31.0 38 31.0 39 34.5 40 38.0 41 52.0 42 66.0 43 66.0 44 56.5 45 47.0 46 67.5 47 88.0 48 88.0 49 100.0 50 112.0 51 107.0 52 102.0 53 102.0 54 120.5 55 139.0 56 136.5 57 134.0 58 134.0 59 129.0 60 124.0 61 120.0 62 116.0 63 116.0 64 107.0 65 98.0 66 104.5 67 111.0 68 111.0 69 72.5 70 34.0 71 25.5 72 17.0 73 17.0 74 10.5 75 4.0 76 6.0 77 8.0 78 8.0 79 5.5 80 3.0 81 2.5 82 2.0 83 2.0 84 1.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.07401924500370095 8 0.07401924500370095 9 0.1480384900074019 10 0.22205773501110287 11 0.0 12 0.0 13 0.07401924500370095 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.07401924500370095 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.07401924500370095 26 0.07401924500370095 27 0.2960769800148038 28 0.07401924500370095 29 0.1480384900074019 30 0.0 31 0.0 32 0.44411547002220575 33 0.0 34 0.0 35 0.0 36 0.1480384900074019 37 0.0 38 0.0 39 0.0 40 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 40 1351.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 91.78386380458919 #Duplication Level Percentage of deduplicated Percentage of total 1 94.35483870967742 86.60251665433013 2 4.354838709677419 7.994078460399703 3 0.7258064516129032 1.9985196150999258 4 0.24193548387096775 0.8882309400444115 5 0.0 0.0 6 0.08064516129032258 0.44411547002220575 7 0.0 0.0 8 0.08064516129032258 0.5921539600296076 9 0.0 0.0 >10 0.16129032258064516 1.4803849000740192 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC 10 0.7401924500370096 No Hit GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTT 10 0.7401924500370096 No Hit GTCCCGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT 8 0.5921539600296076 No Hit GTCCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6 0.44411547002220575 No Hit GTCCCGGGGGTTCTTTGGGATATAGCATTCTTCTCATGGT 4 0.2960769800148038 No Hit GTCCCGGGGGGGCTGGTGAGATGGCTCAGCGGTTAAGAGC 4 0.2960769800148038 No Hit GTCCCGGGGATTTCCACTATGTTCTATCAATGGGAGCAGT 4 0.2960769800148038 No Hit GTCCCGGGGTGGGGGCTTGGTAGCCCTTTCTGCTAGGGAC 3 0.22205773501110287 No Hit GTCCCGGGGATAGCAGGATTTGTTCACTGATTCCCATTAT 3 0.22205773501110287 No Hit GTCCCGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGC 3 0.22205773501110287 No Hit GTCCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG 3 0.22205773501110287 No Hit GTCCCGGGGATCGTTTTAAATGAGAATGCAAAATAAGGTA 3 0.22205773501110287 No Hit GTCCCGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAG 3 0.22205773501110287 No Hit GTCCCGGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAA 3 0.22205773501110287 No Hit GTCCCGGGGATTTTATGGATGCTAGTCATCTTGAAAGATT 3 0.22205773501110287 No Hit GTCCCGGGGCATGGATTACAGTTTTTTAAAAACTAGAATT 3 0.22205773501110287 No Hit GTCCCGGGGATTTTCATGAGATACTCAGTTTTGTCCTCCA 2 0.1480384900074019 No Hit GTCCCGGGGATTGCTGGTGGCTCTAAGATGCAGTATAATT 2 0.1480384900074019 No Hit GTCCCGGGGGTGTGGGGGATCTATGGCCATCTCCTACCAT 2 0.1480384900074019 No Hit GTCCCGGGGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGC 2 0.1480384900074019 No Hit GTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGA 2 0.1480384900074019 No Hit GTCCCGGGGGTTTTAAGAAGATATATTCCTATAGTACACT 2 0.1480384900074019 No Hit GTCCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2 0.1480384900074019 No Hit GTCCCGGGGATGCCACAACTAGATACATCAACATGATTTA 2 0.1480384900074019 No Hit GTCCCGGGGGAGGTGATCAAGCTGAAAGGTTACACATCCT 2 0.1480384900074019 No Hit GTCCCGGGGCTATAGTATAAGTTTGAAATTTCGGTTGGGG 2 0.1480384900074019 No Hit GTCCCGGGGATCTGGGTCTTCAGTACTCTGGGAAACAGAT 2 0.1480384900074019 No Hit GTCCCGGGGGACCCGGGGAGGTAGTGACGAAAAATAACAA 2 0.1480384900074019 No Hit GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGC 2 0.1480384900074019 No Hit GTCCCGGGGGGTTGTATTCGATGTTCTCTGTTTAGGCCCA 2 0.1480384900074019 No Hit GTCCCGGGGATTCTTGCTCATGTGGGACCAACGGCCAGTA 2 0.1480384900074019 No Hit GTCCCGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG 2 0.1480384900074019 No Hit GTCCCGGGGTCATGAATGGCCATTCTACCCAGATAGGTTT 2 0.1480384900074019 No Hit GTCCCGGGGGTCTTCAAAGTCAAATCTTACACGGTCTTCA 2 0.1480384900074019 No Hit GTCCCGGGGGGGCTGGAGAGGTGGCTCAGCAGATAAGAGC 2 0.1480384900074019 No Hit GTCCCGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGC 2 0.1480384900074019 No Hit GTCCCGGGGCTGGGTAGTGATTTCAGTCATCTCAGAAGAC 2 0.1480384900074019 No Hit GTCCCGGGGGGGCTGGTGAGATGGCTCACTGGGTAAGAGC 2 0.1480384900074019 No Hit GTCCCGGGGGATCCAAAAAGATAAAAGGAACTCGGCAAAC 2 0.1480384900074019 No Hit GTCCCGGGGGTCTGGTTCCCAGGATAAGACTGTTAGATTC 2 0.1480384900074019 No Hit GTCCCGGGGGGTTTTACTATTATTTGTTTTCAATGTATAA 2 0.1480384900074019 No Hit GTCCCGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2 0.1480384900074019 No Hit GTCCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACT 2 0.1480384900074019 No Hit GTCCCGGGGGGATTTTTTTCTGTTTTTGTTACCAACTTCC 2 0.1480384900074019 No Hit GTCCCGGGGGACAGGATCAACCATAGGAAAGAAGATTCCT 2 0.1480384900074019 No Hit GTCCCGGGGGGGCTGGTGCGATGGCTCAGTGGGTAAGAGC 2 0.1480384900074019 No Hit GTCCCGGGGGGGCTAGGGAGATGGCTCAGCAGTTAACAAC 2 0.1480384900074019 No Hit GTCCCGGGGTCACCGATCCTCTTCTGAGATGAGTTTTTGT 2 0.1480384900074019 No Hit GTCCCGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2 0.1480384900074019 No Hit GTCCCGGGGGGGGATGGTTTGATCTCACGGCTTTGCCTGA 2 0.1480384900074019 No Hit GTCCCGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG 2 0.1480384900074019 Illumina Single End Adapter 2 (97% over 34bp) GTCCCGGGGGGGTTATTTGGTTAGGATTTTTCAAAATGAT 2 0.1480384900074019 No Hit GTCCCGGGGGGGCCTATGTCGAGGGCCCCAAATTTACAAT 2 0.1480384900074019 No Hit GTCCCGGGGCAGCAGTGTATCACCTTCAGAGGGGCATCGT 2 0.1480384900074019 No Hit GTCCCGGGGGGAGAGGGAGCCTGAGAAACGGCTACCACAT 2 0.1480384900074019 No Hit GTCCCGGGGGCCGTCCTATAGATTTCAGCTATGGACAGAA 2 0.1480384900074019 No Hit GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCT 2 0.1480384900074019 No Hit GTCCCGGGGGAGGAGAAGCTGATGATGCTCCAACATAAGA 2 0.1480384900074019 No Hit GTCCCGGGGGGTTAGACTTTGGGCAGCACATGGTTGACCT 2 0.1480384900074019 No Hit GTCCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCA 2 0.1480384900074019 No Hit GTCCCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT 2 0.1480384900074019 No Hit GTCCCGGGGCTGTCCCGGGGGCTTAAAACAAGCTACATGG 2 0.1480384900074019 No Hit GTCCCGGGGAGGGGTTTTGTTGATAAATCAAGTCTTGGTT 2 0.1480384900074019 No Hit GTCCCGGGGACAGGAATATGGGGTGTCTGGTTGCATAACA 2 0.1480384900074019 No Hit GTCCCGGGGGAGATGAGATGACCTCCTTGTCAGAGTATGT 2 0.1480384900074019 No Hit GTCCCGGGGGGTCCTTTATTTATAAGTGCTTATCCAGTAG 2 0.1480384900074019 No Hit GTCCCGGGGGGATCCTCTAGAGTCGACCTGCAGGCATGCA 2 0.1480384900074019 No Hit GTCCCGGGGACCTTAAATTCAAAACTCTATGAGCAGCCAA 2 0.1480384900074019 No Hit GTCCCGGGGGACAGGATCAACCATAGGAAAGGAGATTCCT 2 0.1480384900074019 No Hit GTCCCGGGGGGGATGGTGAAGAGCATTACAGGCAGTTACA 2 0.1480384900074019 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.07401924500370097 0.0 0.0 0.0 0.0 13 0.07401924500370097 0.0 0.0 0.0 0.0 14 0.07401924500370097 0.0 0.0 0.0 0.0 15 0.07401924500370097 0.0 0.0 0.0 0.0 16 0.07401924500370097 0.0 0.0 0.0 0.0 17 0.07401924500370097 0.0 0.0 0.0 0.0 18 0.07401924500370097 0.0 0.0 0.0 0.0 19 0.07401924500370097 0.0 0.0 0.0 0.0 20 0.07401924500370097 0.0 0.0 0.0 0.0 21 0.07401924500370097 0.0 0.0 0.0 0.0 22 0.07401924500370097 0.0 0.0 0.0 0.0 23 0.07401924500370097 0.0 0.0 0.0 0.0 24 0.07401924500370097 0.0 0.0 0.0 0.0 25 0.07401924500370097 0.0 0.0 0.0 0.0 26 0.07401924500370097 0.0 0.0 0.0 0.0 27 0.07401924500370097 0.0 0.0 0.0 0.0 28 0.07401924500370097 0.0 0.0 0.07401924500370097 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGGGGG 40 2.0676453E-8 33.74074 5 CGGGGGA 30 6.96821E-6 33.74074 5 CCCGGGG 125 0.0 33.74074 3 GTCCCGG 125 0.0 33.74074 1 CCGGGGG 80 0.0 33.74074 4 CCGGGGC 20 0.0019914897 33.74074 4 TCCCGGG 125 0.0 33.74074 2 CGGGGAT 25 1.2008052E-4 33.740738 5 CCGGGGA 25 1.2008052E-4 33.740738 4 >>END_MODULE