FastQCFastQC Report
Fri 17 Jun 2016
SRR1525950_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525950_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129534
Sequences flagged as poor quality0
Sequence length56
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG51273.9580341840752236No Hit
TATGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG13381.0329334383250728No Hit
TATGTAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT6410.4948507727700835No Hit
TATGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG6220.4801828091466333No Hit
TATGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG5670.43772291444717215No Hit
TATGTAGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT5060.39063103123504256No Hit
TATGTAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC4880.3767350656970371No Hit
TATGTAGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC4170.32192320163045995No Hit
TATGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG3940.30416724566523073No Hit
TATGTAGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC3170.24472339308598515No Hit
TATGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC2920.22542344094986644No Hit
TATGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCGTCTTCTGCTTGGATC2860.22079145243719794No Hit
TATGTAGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA2630.20303549647196878No Hit
TATGTAGGGGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCAT2470.19068352710485278No Hit
TATGTAGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT2400.18527954050673953No Hit
TATGTAGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC2320.17910355582318158No Hit
TATGTAGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC2170.16752358454151034No Hit
TATGTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC1820.14050365155094416No Hit
TATGTAGGGATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGT1820.14050365155094416No Hit
TATGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTCCTTCC1780.13741565920916515No Hit
TATGTAGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATG1640.12660768601293867No Hit
TATGTAGGGGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGC1610.12429169175660444No Hit
TATGTAGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGT1590.12274769558571494No Hit
TATGTAGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGG1580.12197569750027021No Hit
TATGTAGGGATCAGATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGG1500.11579971281671221No Hit
TATGTAGGGGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCC1480.11425571664582272No Hit
TATGTAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC1450.11193972238948846No Hit
TATGTAGGGAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTG1450.11193972238948846No Hit
TATGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC1330.10267574536415149No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTCA1850.050.7156441
CAAATAA203.8873716E-450.71563341
CTGAATT150.0080413550.57619539
CGTAGAG150.0080413550.57619539
GCGTAGA150.008053848550.55634338
AGCGTAG150.00810398950.4770737
ACGCAAC150.00810398950.4770737
CAGCGTA150.00814174650.41777836
CGCTTAG150.00823035450.2799742
TCTACAC150.00825580250.24073443
CCGACTC301.0066378E-650.24073443
GCGAGTG204.07223E-450.24073443
TAAGCAT204.0879447E-450.20155746
CACGATT507.2759576E-1250.14291447
TCGTTGA150.008332496550.12339414
CGTTGAT150.008332496550.12339415
GTGATAT150.008332496550.12339415
ACCTAGT150.0083839250.0454711
GATTATG150.00842264349.9871950
GATTACG355.2508767E-849.98718650
Produced by FastQC (version 0.11.5)