##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1525950_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	129534
Sequences flagged as poor quality	0
Sequence length	56
%GC	50
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.493854895239856	38.0	36.0	39.0	32.0	40.0
2	33.852131486713915	38.0	34.0	39.0	16.0	40.0
3	35.16858122191857	38.0	35.0	39.0	27.0	40.0
4	35.17319777046953	38.0	35.0	39.0	27.0	40.0
5	35.01315484737598	38.0	34.0	39.0	25.0	40.0
6	32.0963299211018	38.0	27.0	39.0	12.0	40.0
7	35.663254435129	38.0	35.0	39.0	30.0	40.0
8	35.60928404897556	38.0	35.0	39.0	29.0	40.0
9	35.55232603023145	38.0	35.0	39.0	29.0	40.0
10	34.50237775410317	38.0	33.0	39.0	24.0	40.0
11	34.07000478638813	38.0	33.0	39.0	23.0	40.0
12	33.069564747479426	38.0	31.0	39.0	19.0	40.0
13	32.52741365201414	37.0	30.0	39.0	17.0	40.0
14	33.1301897571294	38.0	31.0	39.0	19.0	40.0
15	32.06379792178115	36.0	29.0	39.0	15.0	40.0
16	32.69058316735374	37.0	30.0	39.0	19.0	40.0
17	31.82695662914756	36.0	28.0	39.0	16.0	40.0
18	31.186352617845507	36.0	27.0	39.0	14.0	40.0
19	32.220374573471055	36.0	29.0	38.0	18.0	40.0
20	32.88694860036747	37.0	30.0	39.0	20.0	40.0
21	33.1597804437445	38.0	31.0	39.0	19.0	40.0
22	32.524178980036126	37.0	30.0	39.0	17.0	40.0
23	33.185789059243135	38.0	31.0	39.0	19.0	40.0
24	32.5020843948307	37.0	30.0	39.0	17.0	40.0
25	32.95393487424151	37.0	31.0	39.0	19.0	40.0
26	33.518103355103676	38.0	33.0	39.0	20.0	40.0
27	33.76839285438572	38.0	33.0	39.0	22.0	40.0
28	33.553159788163725	38.0	33.0	39.0	22.0	40.0
29	33.151218984976914	38.0	32.0	39.0	19.0	40.0
30	32.84737597850757	37.0	31.0	39.0	18.0	40.0
31	33.604381861132985	38.0	33.0	39.0	20.0	40.0
32	33.518527954050676	38.0	33.0	39.0	21.0	40.0
33	33.309748791823	38.0	33.0	39.0	19.0	40.0
34	33.186661417079684	38.0	33.0	39.0	19.0	40.0
35	33.190915126530484	38.0	33.0	39.0	19.0	40.0
36	32.360939984868835	36.0	31.0	39.0	15.0	40.0
37	32.145992557938456	36.0	31.0	38.0	11.0	40.0
38	31.4011456451588	36.0	30.0	38.0	5.0	40.0
39	30.251208177003722	35.0	27.0	38.0	4.0	40.0
40	28.35405376194667	34.0	21.0	38.0	4.0	40.0
41	26.256179844673984	31.0	16.0	38.0	4.0	39.0
42	27.176224002964474	32.0	20.0	38.0	4.0	39.0
43	27.305896521376628	33.0	20.0	38.0	4.0	39.0
44	26.20437105315979	31.0	17.0	37.0	4.0	39.0
45	27.239635925702903	33.0	21.0	38.0	4.0	39.0
46	25.14336004446709	30.0	15.0	36.0	4.0	39.0
47	24.301187333055413	29.0	10.0	36.0	4.0	39.0
48	24.956073308938194	30.0	14.0	36.0	4.0	39.0
49	23.85658591566693	28.0	8.0	35.0	4.0	39.0
50	24.51212036994148	29.0	10.0	36.0	4.0	39.0
51	24.76278814828539	30.0	4.0	36.0	4.0	39.0
52	24.60412710176479	30.0	4.0	36.0	4.0	39.0
53	24.35651643583924	30.0	4.0	36.0	4.0	39.0
54	24.037326107431255	30.0	4.0	36.0	4.0	39.0
55	23.73818456930227	30.0	4.0	36.0	4.0	39.0
56	22.843685827659147	28.0	4.0	36.0	4.0	38.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	3.3212651689641817
1	2	6.5312323082006465
1	3	5.162940188331035
1	4	5.123969005618491
1	5	5.36632120652186
1	6	8.989150274637389
1	7	4.37666827300486
1	8	4.367024212211163
1	9	4.3916598534698394
1	10	5.542294506164161
1	11	1.5183988187598487
1	12	-6.550464262678602
1	13	-17.548671655377966
1	14	-6.268081187982126
1	15	1.8633570892343982
1	16	-1.1539143017428017
1	17	-5.99208538241766
1	18	-5.152332092883928
1	19	-0.1434777125465203
1	20	-0.9664826302759408
1	21	5.698916280879054
1	22	6.67977234931201
1	23	6.14596073191494
1	24	6.747706703913217
1	25	5.570021438589951
1	26	5.66794430709356
1	27	5.366372449117037
1	28	5.577282887062825
1	29	6.224375199535579
1	30	6.313409377862019
1	31	5.174875651561358
1	32	4.978104925229406
1	33	5.007594322527122
1	34	4.973235021414723
1	35	4.688189929364356
1	36	4.081121059073411
1	37	4.029845289439386
1	38	4.559943762316866
1	39	4.657385502551179
1	40	-10.25207118526932
1	41	-0.5010467582553062
1	42	-2.4611336800203283
1	43	-9.34802231622696
1	44	-14.959089287461005
1	45	-3.00937858393608
1	46	-5.0404176249907415
1	47	-10.257502076002716
1	48	-5.709801913053951
1	49	-13.527073737561
1	50	-3.9428543969861884
1	51	1.57438186236433
1	52	1.4966297016924877
1	53	1.7919813411340044
1	54	1.4624045628556068
1	55	1.4402221704467166
1	56	5.122363218347434
2	1	3.9156927272215256
2	2	7.066693301108447
2	3	5.905696014065583
2	4	5.53876130551717
2	5	5.90102232101021
2	6	9.417974996015303
2	7	4.746931697523607
2	8	4.581512560742063
2	9	4.9267662364485645
2	10	6.086012844563349
2	11	5.2552275928226635
2	12	0.8493837616375046
2	13	2.5030000771448293
2	14	6.01596136520936
2	15	6.7527187913620565
2	16	4.66351224334332
2	17	6.836587363276365
2	18	4.57416233467433
2	19	5.745326745406874
2	20	5.487266103260023
2	21	6.631793687160716
2	22	7.589194841713223
2	23	6.484157287132774
2	24	7.00566009803682
2	25	6.317336534841214
2	26	6.164955451977047
2	27	5.838510240403764
2	28	5.945216017761911
2	29	6.539674085047231
2	30	6.1933485875884635
2	31	4.722241406373918
2	32	5.527547681055136
2	33	5.08748287369227
2	34	4.571816581698414
2	35	4.9454341036298075
2	36	6.13274213303491
2	37	6.022195846683559
2	38	7.276711746106127
2	39	6.926990365773065
2	40	5.636783931245368
2	41	10.11769690942453
2	42	8.278025387862144
2	43	7.771329254188444
2	44	-2.7391804728713396
2	45	7.983579876043656
2	46	5.979693823844109
2	47	3.196550608901031
2	48	6.475963031221632
2	49	-1.3826461793036557
2	50	6.529587345668322
2	51	8.91146392923363
2	52	9.160155537558747
2	53	9.216348109117792
2	54	9.159517024861689
2	55	8.794629465279545
2	56	10.18756078673649
3	1	3.7342572544817045
3	2	6.595678808354823
3	3	5.756834730421687
3	4	5.043385184054095
3	5	5.533527496993649
3	6	9.334124064338283
3	7	4.603798502216499
3	8	4.773438026580571
3	9	4.927249328236002
3	10	5.712789932210001
3	11	5.687249677018663
3	12	6.199073202631297
3	13	6.284918641672089
3	14	6.406782621248009
3	15	1.9589989845987716
3	16	6.7131326712246135
3	17	7.795662587568977
3	18	8.103423894370675
3	19	6.6521590435435165
3	20	6.207970037007438
3	21	7.191490029465754
3	22	7.619077519421985
3	23	6.907138653592405
3	24	7.414424763323225
3	25	6.518371731528585
3	26	6.1262390958693835
3	27	5.8492072728399265
3	28	5.843214637499667
3	29	6.055961179595194
3	30	6.605011803599506
3	31	5.0303849536479035
3	32	5.546457273877774
3	33	5.570781700469357
3	34	5.258773103437541
3	35	5.304440314809931
3	36	7.159726260019035
3	37	7.396660995061751
3	38	7.699141007665826
3	39	8.890965521052571
3	40	10.102898493012106
3	41	13.024598220673667
3	42	11.509495367158209
3	43	11.735028357017981
3	44	12.503676669985804
3	45	11.303731704891135
3	46	13.077554417356879
3	47	13.475846675153623
3	48	13.124893327978018
3	49	13.73253670544445
3	50	12.794528684522703
3	51	12.390138877473795
3	52	12.24310929877338
3	53	12.601648316157128
3	54	12.46690142789826
3	55	12.596147753754359
3	56	11.76885823325133
4	1	3.4935638756809126
4	2	6.591203105030015
4	3	5.735948114905916
4	4	5.298145059018573
4	5	5.380714197760909
4	6	9.164473595455078
4	7	4.41233785998859
4	8	4.431792672786884
4	9	4.913040746252484
4	10	5.25015850282665
4	11	5.680997900945911
4	12	6.56394958796804
4	13	6.874148536528583
4	14	6.662039796581912
4	15	7.068405065871865
4	16	6.994604680318112
4	17	7.743730220419216
4	18	8.609036284254163
4	19	7.693506017115556
4	20	6.464577027629776
4	21	6.4686284210542695
4	22	7.48317243274964
4	23	6.651384177889078
4	24	7.075407997196486
4	25	6.38218247321657
4	26	5.31187421948405
4	27	5.075905198386955
4	28	4.958801451935582
4	29	5.803119463198492
4	30	5.925841584787346
4	31	4.528964095449545
4	32	4.904158325312839
4	33	4.779079512347732
4	34	4.83848324836508
4	35	4.8749859243581
4	36	5.824758939757601
4	37	5.988022177215775
4	38	6.1732103455459075
4	39	7.030351710310882
4	40	1.0631144634582554
4	41	10.678761335194842
4	42	9.130126228198279
4	43	9.035895080518976
4	44	10.25857863077012
4	45	8.961731136547854
4	46	12.183195224404336
4	47	12.106494586492072
4	48	11.528203927580176
4	49	11.586401439743963
4	50	12.199615356872805
4	51	10.632892500662201
4	52	11.10301268041589
4	53	11.008084803795665
4	54	10.925483411416312
4	55	10.886926384047051
4	56	10.465291879173463
5	1	3.823911243054255
5	2	6.695288901785265
5	3	5.848867394292796
5	4	5.176566478347723
5	5	5.625339459032936
5	6	9.136007386636528
5	7	4.626506268468738
5	8	4.421565739373925
5	9	4.942502277057699
5	10	5.530441174270862
5	11	5.799341685160108
5	12	6.5482235005787714
5	13	6.6436817305168425
5	14	6.665199943889565
5	15	8.055764476641244
5	16	6.996047897198771
5	17	7.7940686298946815
5	18	8.258160400905897
5	19	7.261884637599284
5	20	6.772231053707213
5	21	6.967060788925281
5	22	7.403770621263625
5	23	6.513333018338962
5	24	7.009020020686087
5	25	6.061813208759514
5	26	5.0512740403260885
5	27	5.146100281496338
5	28	5.057761340459518
5	29	5.809265576466132
5	30	5.68029565067766
5	31	4.431571839054605
5	32	4.232938060557192
5	33	4.02581983284141
5	34	4.621901184067276
5	35	4.710384550813757
5	36	6.140549723075999
5	37	6.031816344628936
5	38	6.220463256862718
5	39	7.514227494126949
5	40	8.578094059357525
5	41	11.531354148970095
5	42	10.802366697990252
5	43	10.16272398466947
5	44	11.569666517701538
5	45	9.89987187507677
5	46	12.0581081337305
5	47	11.618861462176351
5	48	11.791143113406793
5	49	12.481917514194048
5	50	11.451680649995126
5	51	10.73568437883479
5	52	10.671540599197616
5	53	10.642478155044795
5	54	11.307015211953786
5	55	11.041475005526102
5	56	11.440135115960565
6	1	3.6165585280873316
6	2	6.687905422320565
6	3	5.742781151150716
6	4	5.11870358545945
6	5	5.470286390274282
6	6	9.309749887790197
6	7	4.668576719168627
6	8	4.333604912834417
6	9	4.964139973822299
6	10	5.424252382802884
6	11	6.122471460066535
6	12	6.528893141146888
6	13	7.464760538905292
6	14	6.842223991471986
6	15	7.984031922675186
6	16	7.381116860947934
6	17	8.06443729112629
6	18	9.257134917646912
6	19	7.800160800240928
6	20	6.7738461898401106
6	21	6.851129906496936
6	22	7.264663817182196
6	23	6.323695526671017
6	24	7.523120415497139
6	25	6.5519685694732495
6	26	5.957596144617735
6	27	5.2973846992782185
6	28	5.447957720503613
6	29	5.855258500631642
6	30	5.96535435959424
6	31	5.025993210125719
6	32	4.7884423419498
6	33	4.5984496846590766
6	34	5.050604460486291
6	35	5.118449976645685
6	36	6.48295858325136
6	37	6.03763596212368
6	38	6.649294718689102
6	39	7.96826020704291
6	40	4.666798361259801
6	41	11.819284211011833
6	42	10.831849341686095
6	43	10.94780833066752
6	44	11.291555457864593
6	45	10.51936631183009
6	46	12.525292669442955
6	47	13.202899815250237
6	48	12.386641241899842
6	49	13.265694369036897
6	50	12.900150116231089
6	51	12.180872298642
6	52	12.587861165264375
6	53	12.059926752696438
6	54	12.747973013317672
6	55	12.429261499911583
6	56	11.603289502465206
7	1	3.8250684982553267
7	2	6.870665667256976
7	3	5.823065863559783
7	4	5.3466566916508995
7	5	5.645039098705745
7	6	9.39194489485567
7	7	4.59376120652928
7	8	4.689259908385786
7	9	5.08686986339157
7	10	5.665682225268995
7	11	6.245358360155507
7	12	6.627548084361415
7	13	7.310550040135208
7	14	6.726324059509881
7	15	8.028884594470867
7	16	7.431412564093378
7	17	8.166441792094524
7	18	8.666513679357283
7	19	7.524502664479169
7	20	6.257831116830221
7	21	7.126735705418795
7	22	7.819172635939722
7	23	6.856301377929718
7	24	7.282763478249009
7	25	6.78096841246273
7	26	6.140356389554146
7	27	5.468290649976915
7	28	5.808872568465095
7	29	6.102393058647031
7	30	5.935391518750567
7	31	4.956018855177277
7	32	5.176153651940904
7	33	5.012748109320196
7	34	5.36007219482449
7	35	5.346717103876543
7	36	7.232840825361578
7	37	7.256639468691951
7	38	7.994377672580345
7	39	8.428248692938126
7	40	8.13636448885702
7	41	12.033734412508664
7	42	11.15288351764255
7	43	11.029113611454662
7	44	12.20833988242104
7	45	10.40432993772636
7	46	12.752356049356074
7	47	13.579698894121858
7	48	13.160912451404212
7	49	13.550563788621343
7	50	12.435163462125267
7	51	12.141959858175156
7	52	12.21700231772159
7	53	12.369839350173802
7	54	12.253965581486408
7	55	12.341335608876882
7	56	11.67734613092598
8	1	3.789350275347161
8	2	6.839742946498514
8	3	5.673578486203375
8	4	5.182479643916359
8	5	5.541853121415478
8	6	9.084894955488458
8	7	4.328795932579332
8	8	4.648939105828184
8	9	4.700170491767707
8	10	5.802932804036502
8	11	5.83222860599389
8	12	6.917620843704377
8	13	7.259838982919906
8	14	7.008514556698724
8	15	7.613357089234398
8	16	6.771617613150816
8	17	7.667489085667448
8	18	8.459370034775649
8	19	7.0586499470279485
8	20	6.342028008021931
8	21	6.869129046836498
8	22	7.238282987609878
8	23	6.316173497872391
8	24	7.247706703913217
8	25	6.463638459866548
8	26	5.7743272858169625
8	27	4.914244789542565
8	28	5.141112674296867
8	29	5.660545412301531
8	30	5.669792356585429
8	31	4.052535226029441
8	32	4.164275137995357
8	33	4.4331262374207405
8	34	4.122171191627487
8	35	4.624360142130314
8	36	5.421546590988299
8	37	5.311760183056407
8	38	5.7620714218913385
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115	25	4.979762130079312
115	26	4.143675690072282
115	27	3.866040002308523
115	28	4.090082089190485
115	29	3.5643020612377043
115	30	4.926607516159894
115	31	3.603565811135823
115	32	3.3009107762932324
115	33	2.8362179927398863
115	34	3.637962415031744
115	35	3.249526365534571
115	36	4.921879037796813
115	37	4.348163108588324
115	38	5.2306551984870815
115	39	5.592392151487353
115	40	7.102483336007275
115	41	8.025881433234055
115	42	6.741492649766904
115	43	5.062549492283679
115	44	0.2771141699858042
115	45	5.278520352234132
115	46	1.021583704796491
115	47	-8.100919629194205
115	48	1.6450850550311564
115	49	-3.3537027269227018
115	50	3.21738496471594
115	51	2.2969550006622015
115	52	2.2748876804158904
115	53	2.9613629900701746
115	54	2.8588473820045444
115	55	3.2372633938509736
115	56	5.5728286438793475
116	1	3.131710043238847
116	2	5.739853474268617
116	3	5.239895148264715
116	4	4.395038361794228
116	5	4.82093574092363
116	6	7.517542095582407
116	7	3.852559403151311
116	8	3.95986753909704
116	9	4.246246755929079
116	10	4.875190333740839
116	11	4.564751402346474
116	12	4.577955190208932
116	13	5.61338968753444
116	14	5.631545780793775
116	15	-1.939489000845736
116	16	3.5643780442091213
116	17	5.333557060246058
116	18	5.0421277026397
116	19	4.892512892593022
116	20	3.845274761268687
116	21	5.921836977204002
116	22	6.347636400154784
116	23	5.893681096656586
116	24	6.677521569898296
116	25	5.612574630079312
116	26	5.56149224851384
116	27	4.405609807503325
116	28	4.095865368411264
116	29	5.273007418380558
116	30	4.849192743432621
116	31	4.049081233213748
116	32	3.9955130490205057
116	33	4.159777245986643
116	34	4.297357707239534
116	35	3.9553908135865186
116	36	5.762179362472139
116	37	6.632152556640271
116	38	6.810910880305261
116	39	8.13997737876008
116	40	10.28440297886442
116	41	10.015532407260032
116	42	7.893176903013661
116	43	3.174359557218743
116	44	1.6595208258299614
116	45	8.222108014571795
116	46	2.0541526983029854
116	47	-4.3389472265968045
116	48	1.9789933342519355
116	49	-1.8887067853642598
116	50	2.8005830166639925
116	51	6.564710682480385
116	52	6.544571121974332
116	53	6.160936853706538
116	54	5.558218323562986
116	55	5.628395699045779
116	56	8.114833514009216
117	1	2.6044373159661163
117	2	5.233359967775112
117	3	4.625609433979001
117	4	4.028804595560466
117	5	4.233922753910647
117	6	6.755204433244742
117	7	3.3473645979565063
117	8	3.478049357278863
117	9	3.30858441826674
117	10	4.214151372701878
117	11	1.2608552984503731
117	12	-3.3428240305702843
117	13	-7.637259663114911
117	14	-0.5840386347906374
117	15	-6.074553935910668
117	16	-6.633024553193476
117	17	0.5452453719343708
117	18	-2.0267034661914707
117	19	-0.26852606844594007
117	20	-0.14433562834170743
117	21	4.603655159022189
117	22	5.196987049505431
117	23	4.383291486266977
117	24	5.355443647820373
117	25	4.885301902806585
117	26	4.19395978098137
117	27	4.0276877295812525
117	28	4.007553680099573
117	29	4.370410015783158
117	30	4.821920016159897
117	31	3.49063967477219
117	32	3.122785776293231
117	33	3.815621401830793
117	34	3.632422642304469
117	35	3.643702501898204
117	36	5.201140401433179
117	37	4.920464244951958
117	38	6.047274516668903
117	39	7.012704651487354
117	40	9.205182199643637
117	41	7.305142796870417
117	42	6.485384695221448
117	43	-4.490575507716322
117	44	-0.5417778754687426
117	45	4.577952170415951
117	46	-2.175717431567147
117	47	-2.468817356466932
117	48	0.1724998277584291
117	49	-1.0666288632863399
117	50	0.14863496471593862
117	51	4.3010743188440195
117	52	5.021194498597708
117	53	4.679118671888357
117	54	5.47120533655
117	55	5.886837257487336
117	56	7.390158189333892
118	1	2.0427989543277576
118	2	4.782810517225659
118	3	4.052582460952031
118	4	3.6427906095464735
118	5	3.792963712951604
118	6	7.1194401974805075
118	7	3.0223895729814814
118	8	3.016311095540601
118	9	3.1278651375474595
118	10	3.7687967273472296
118	11	0.1087074463025175
118	12	-6.1033634911097465
118	13	-5.650846076701324
118	14	-9.943379294131297
118	15	-10.23519329655003
118	16	-11.71634123651016
118	17	-2.442167215478218
118	18	-8.175654515142519
118	19	-2.8187758186956913
118	20	-2.6991807831868613
118	21	3.067691123058154
118	22	3.8056783581967366
118	23	3.009065712041199
118	24	3.559139951516677
118	25	3.1345526520573337
118	26	3.9755781625997564
118	27	3.4300853319788516
118	28	3.4045566771025726
118	29	3.90487555024869
118	30	4.023018917258792
118	31	3.028102212234721
118	32	3.2227857762932324
118	33	2.955981042190434
118	34	3.087567497449328
118	35	3.067778425974133
118	36	5.607833708126485
118	37	5.623661048148762
118	38	5.964257533651914
118	39	7.050067288849988
118	40	8.328359022820464
118	41	7.684363576091197
118	42	5.804765314602069
118	43	0.2248091076682961
118	44	-0.08972992342079067
118	45	6.109220901684683
118	46	-1.0437493995991147
118	47	0.33537844772887127
118	48	-1.1353922801336793
118	49	-0.48411138076885507
118	50	1.1167668328478086
118	51	6.6631122808819825
118	52	5.8502654276686386
118	53	5.802794995564682
118	54	6.089187354532015
118	55	6.383640454290536
118	56	8.206242105417807
119	1	2.3065352180640204
119	2	5.361565095980243
119	3	4.399346807716377
119	4	4.309457276213145
119	5	4.420558340546229
119	6	7.605398683438992
119	7	3.571840122432029
119	8	3.637800717030217
119	9	3.987449997132323
119	10	5.111898070448575
119	11	1.9157892533843253
119	12	-5.809102196848453
119	13	-2.2357056615609068
119	14	-4.523354874106875
119	15	-7.625913687270419
119	16	-9.777391297560222
119	17	-1.2199449932559965
119	18	-7.030355369843374
119	19	-0.22781122773109885
119	20	-0.8737839577900353
119	21	4.380267435634465
119	22	4.877717430235812
119	23	4.221519924495411
119	24	3.715428107804833
119	25	3.830523348028027
119	26	5.40048658750818
119	27	5.074773976667494
119	28	4.436302708848604
119	29	5.394497039870181
119	30	5.54927044351032
119	31	4.579994764127278
119	32	4.479196032703488
119	33	4.586017672227069
119	34	4.052158462040289
119	35	4.122723480919184
119	36	5.629811730104507
119	37	6.500339924827642
119	38	7.171827741222128
119	39	7.395610634393336
119	40	9.739836434297871
119	41	7.652617544345166
119	42	7.2467677566045126
119	43	-3.7068148239556358
119	44	0.3583775246866594
119	45	6.371736164199945
119	46	-1.7983281541778666
119	47	-2.391117278766856
119	48	-4.0181761629175625
119	49	-2.749068645726119
119	50	0.7785494946304716
119	51	6.6179352357049375
119	52	7.068824646227856
119	53	6.955420148189834
119	54	6.35170261704728
119	55	7.310380381030459
119	56	9.924190823366526
120	1	2.6252165367453344
120	2	5.474431396346542
120	3	4.611648395017966
120	4	4.219713686469554
120	5	4.21054613053402
120	6	7.4298797579200695
120	7	3.3314555070474157
120	8	3.514250655980156
120	9	3.6196233793057075
120	10	4.674703320753821
120	11	0.9843942594893349
120	12	-4.672044809791064
120	13	-3.827357065712313
120	14	-5.614395777647779
120	15	1.611647362790631
120	16	-9.67101156618049
120	17	-0.8604364462474479
120	18	-7.909170998659004
120	19	-1.3915780164978884
120	20	-1.3722577062637846
120	21	2.941317496684526
120	22	4.52133769885608
120	23	3.768681096656586
120	24	3.756255336132064
120	25	2.871503201507881
120	26	4.511979261500851
120	27	3.6897007165942384
120	28	3.8523588749047697
120	29	4.924793132666274
120	30	4.1933485875884635
120	31	3.6984318825643925
120	32	3.3567143477218018
120	33	3.004744778454171
120	34	3.2908642007460287
120	35	2.5341245798202863
120	36	3.910718323511098
120	37	4.193029180016893
120	38	4.950521269915651
120	39	5.894847508630207
120	40	6.9272601217215595
120	41	-5.387064995337372
120	42	-2.7261412788045227
120	43	-7.194146936287748
120	44	-14.02310904429991
120	45	-3.0205867906230104
120	46	-6.314353795203509
120	47	-6.089758914908492
120	48	-3.8984417306831283
120	49	-13.839193798351273
120	50	-2.9745793209983447
120	51	1.7043210720907744
120	52	1.3619412518444634
120	53	2.2705147757844593
120	54	1.5452313105759714
120	55	1.7517723224224007
120	56	4.4947036438793475
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	3991.0
5	156.0
6	411.0
7	370.0
8	247.0
9	237.0
10	256.0
11	308.0
12	257.0
13	256.0
14	311.0
15	440.0
16	584.0
17	772.0
18	970.0
19	1341.0
20	1729.0
21	2357.0
22	2801.0
23	3161.0
24	3706.0
25	3927.0
26	4250.0
27	4338.0
28	4640.0
29	4749.0
30	5145.0
31	5791.0
32	6358.0
33	7085.0
34	7980.0
35	8555.0
36	9664.0
37	10698.0
38	11074.0
39	10615.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	0.0	100.0	0.0
4	100.0	0.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	100.0	0.0	0.0
7	99.3098337116124	0.26247934905121434	0.413790973798385	0.013895965538005466
8	99.6703568175151	0.2578473605385459	0.044775888955795394	0.02701993299056618
9	98.55015401718508	0.3574433919293451	0.35589935999876476	0.7365032308868148
10	51.34096067441752	21.36813500702518	17.94200750382139	9.3488968147359
11	34.364722775487515	30.3981966124724	17.49038862383621	17.74669198820387
12	27.372736115614433	17.499652600861552	19.7191470965152	35.40846418700882
13	22.106165176710363	13.56786635169145	23.40003396791576	40.925934503682434
14	21.824385875523028	22.40956042429015	33.02067410872821	22.745379591458615
15	34.43902287660288	17.37892621927096	22.977449208356283	25.204601695769885
16	35.8601776747779	21.43993820007725	21.16724604094245	21.532638084202393
17	25.46548355458765	17.5852775139201	36.57299075597155	20.3762481755207
18	23.27342628190282	18.68003767350657	32.04255253446971	26.003983510120893
19	34.03459993821439	20.294253938832252	19.688755020080322	25.98239110287303
20	24.72267959153699	29.75348438115999	24.126682281641582	21.397153745661434
21	22.723167069395444	20.22650598689137	31.811970694726437	25.238356248986744
22	21.443018821313323	17.79918785801411	27.82898698411228	32.928806336560285
23	21.500146679636234	20.826964349128414	24.077076288850805	33.59581268238455
24	20.72788474900792	20.85141207170761	31.413770208297947	27.00693297098652
25	32.94959696099324	20.76114148058927	23.74301244633868	22.54624911207882
26	27.49277847289803	20.646616309065912	20.578648995165054	31.281956222871
27	22.87711978985591	22.621392977170007	19.23127438482636	35.27021284814772
28	24.400318080400226	30.63299543724474	21.48200761231249	23.48467887004254
29	36.869253195783806	22.337620156057874	19.12366310677359	21.669463541384722
30	27.53756421646259	19.87330526478427	32.100119741975355	20.489010776777782
31	27.964760294333384	30.562955070147396	21.10444511361794	20.367839521901278
32	37.94421535467729	22.03354491961266	19.740073205763796	20.282166519946255
33	28.24942606033903	21.580131252463882	20.259876786556287	29.9105659006408
34	23.30076810618894	30.530867348912043	26.0382583404884	20.130106204410616
35	22.968490237773054	22.564855982988593	35.49739029576648	18.969263483471874
36	26.701376550743895	30.067050844315357	22.414911860583683	20.81666074435707
37	26.322504813677806	20.20507427369528	32.86060053047116	20.611820382155756
38	35.259887486554874	23.271866221978037	21.31410132400622	20.154144967460862
39	26.389297038125438	21.188616502977343	20.17632047018792	32.2457659887093
40	21.15129887332284	17.821913325333874	28.91493042028331	32.111857381059984
41	20.01890049125657	15.253946063308835	25.82650598645725	38.900647458977346
42	30.054289321034982	22.576148323691363	22.686120985417006	24.68344136985665
43	19.858134060215587	18.72289679098005	33.33539833973485	28.083570809069506
44	22.03880256615132	21.218844301018546	23.44754827048538	33.294804862344755
45	23.601801829693038	20.737295088311328	32.63029984055974	23.03060324143589
46	19.991788229278836	24.421496246601023	23.87533602410852	31.71137950001162
47	17.50776156473145	27.4379074821484	24.61036945048122	30.443961502638935
48	22.73141709163962	28.955339205686908	24.41585535465925	23.897388348014218
49	20.660683449616304	28.74189826466056	22.397571608886548	28.199846676836586
50	30.12342812923196	22.434513445540723	24.384600502998648	23.05745792222867
51	21.88940627735982	29.880604647198744	26.270076781826496	21.959912293614945
52	22.39044995976976	23.806244971219908	31.672804357244537	22.130500711765798
53	22.28812575012583	22.069766541484377	32.490611328351854	23.15149638003794
54	22.936198874325488	28.88508674816323	25.593998467100732	22.584715910410555
55	21.57732908912072	29.28274355816817	25.341357063748383	23.798570288962722
56	29.124409692653092	20.426569636912596	26.84446852984439	23.60455214058992
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	0.5
5	0.0
6	21.0
7	42.0
8	63.5
9	85.0
10	110.5
11	136.0
12	143.5
13	154.5
14	158.0
15	179.0
16	200.0
17	197.5
18	195.0
19	210.0
20	225.0
21	254.0
22	335.0
23	387.0
24	431.0
25	475.0
26	524.0
27	573.0
28	652.0
29	833.5
30	936.0
31	1088.0
32	1240.0
33	1416.0
34	1592.0
35	1737.5
36	1883.0
37	2109.0
38	2741.5
39	3148.0
40	4337.0
41	5526.0
42	9268.5
43	13011.0
44	11844.5
45	10678.0
46	9205.5
47	7341.0
48	6949.0
49	7430.5
50	7912.0
51	7955.5
52	7999.0
53	7774.0
54	7698.5
55	7848.0
56	8136.5
57	8425.0
58	7834.5
59	7244.0
60	6636.5
61	6029.0
62	5674.5
63	4465.0
64	3610.0
65	3193.0
66	2776.0
67	2502.5
68	2229.0
69	1981.0
70	1733.0
71	1250.0
72	587.5
73	408.0
74	375.0
75	342.0
76	415.5
77	489.0
78	318.5
79	86.5
80	25.0
81	15.5
82	6.0
83	3.0
84	0.0
85	0.0
86	0.0
87	0.5
88	0.5
89	0.0
90	0.5
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0023159942563342442
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.4824988034029675
16	0.06484783917735884
17	0.03473991384501366
18	0.0
19	0.0416878966140164
20	0.13201167261105193
21	7.719980854447482E-4
22	0.0
23	0.0
24	0.006175984683557985
25	0.013895965538005466
26	0.04631988512668488
27	0.07642781045903006
28	0.005403986598113236
29	0.17215557305417883
30	0.06870782960458258
31	0.01621195979433971
32	0.027791931076010933
33	0.12737968409838343
34	0.19685951178841074
35	0.16134759985795236
36	0.06175984683557985
37	0.16597958837062085
38	0.23545941606064819
39	0.17292757113962356
40	0.9202217178501398
41	1.1541371377398983
42	0.31729121311779146
43	0.30725523800700977
44	0.4824988034029675
45	0.2563033643676564
46	0.3481711365355814
47	0.5357666712986552
48	0.08800778174070129
49	0.3049392437506755
50	0.23854740840242716
51	0.36052310590269737
52	0.21461546775364
53	0.300307255238007
54	0.28332329735822254
55	0.32192320163045995
56	0.2810073031018883
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
56	129534.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.57841184553862
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.41974393611552	57.10006639183535
2	6.8283702525971	8.819306128120802
3	1.8983634385721628	3.67779887905878
4	0.8499599526604583	2.1955625550048636
5	0.48415440341418514	1.5632961230256148
6	0.2857108701629389	1.1070452545277687
7	0.18887998948010185	0.8538298825018914
8	0.1255215119962702	0.6484783917735883
9	0.13747594171020072	0.7990180184353144
>10	0.6670571780373218	8.194759676996002
>50	0.06574936342661773	3.1034323034878875
>100	0.04184050399875674	5.143051245232912
>500	0.004781771885572199	1.8033875275989315
>1k	0.0011954429713930497	1.0329334383250728
>5k	0.0011954429713930497	3.9580341840752236
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	5127	3.9580341840752236	No Hit
TATGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG	1338	1.0329334383250728	No Hit
TATGTAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT	641	0.4948507727700835	No Hit
TATGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG	622	0.4801828091466333	No Hit
TATGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG	567	0.43772291444717215	No Hit
TATGTAGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT	506	0.39063103123504256	No Hit
TATGTAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC	488	0.3767350656970371	No Hit
TATGTAGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC	417	0.32192320163045995	No Hit
TATGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG	394	0.30416724566523073	No Hit
TATGTAGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC	317	0.24472339308598515	No Hit
TATGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	292	0.22542344094986644	No Hit
TATGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCGTCTTCTGCTTGGATC	286	0.22079145243719794	No Hit
TATGTAGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA	263	0.20303549647196878	No Hit
TATGTAGGGGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCAT	247	0.19068352710485278	No Hit
TATGTAGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT	240	0.18527954050673953	No Hit
TATGTAGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC	232	0.17910355582318158	No Hit
TATGTAGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC	217	0.16752358454151034	No Hit
TATGTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC	182	0.14050365155094416	No Hit
TATGTAGGGATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGT	182	0.14050365155094416	No Hit
TATGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTCCTTCC	178	0.13741565920916515	No Hit
TATGTAGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATG	164	0.12660768601293867	No Hit
TATGTAGGGGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGC	161	0.12429169175660444	No Hit
TATGTAGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGT	159	0.12274769558571494	No Hit
TATGTAGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGG	158	0.12197569750027021	No Hit
TATGTAGGGATCAGATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGG	150	0.11579971281671221	No Hit
TATGTAGGGGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCC	148	0.11425571664582272	No Hit
TATGTAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC	145	0.11193972238948846	No Hit
TATGTAGGGAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTG	145	0.11193972238948846	No Hit
TATGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	133	0.10267574536415149	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	7.719980854447482E-4	0.0	0.0	0.0	0.0
7	7.719980854447482E-4	0.0	0.0	0.0	0.0
8	7.719980854447482E-4	0.0	0.0	0.0	0.0
9	7.719980854447482E-4	0.0	0.0	0.0	0.0
10	7.719980854447482E-4	0.0	0.0	0.0	0.0
11	0.0015439961708894963	0.0	0.0	0.0	0.0
12	0.0046319885126684885	0.0	0.0	0.0	0.0
13	0.005403986598113236	0.0	0.0	0.0	0.0
14	0.015439961708894962	0.0	0.0	0.0	0.0
15	0.016983957879784458	0.0	0.0	0.0	0.0
16	0.017755955965229206	0.0	0.0	0.0	0.0
17	0.02007195022156345	0.0	0.0	0.0	0.0
18	0.022387944477897694	0.0	0.0	0.0	0.0
19	0.02315994256334244	0.0	0.0	0.0	0.0
20	0.02547593681967669	0.0	0.0	0.0	0.0
21	0.027019932990566185	0.0	0.0	0.0	0.0
22	0.027791931076010933	0.0	0.0	0.0	0.0
23	0.02856392916145568	0.0	0.0	0.0	0.0
24	0.030107925332345176	0.0	0.0	0.0	0.0
25	0.030879923417789924	0.0	0.0	0.0	0.0
26	0.03165192150323467	0.0	0.0	0.0	0.0
27	0.03473991384501367	0.0	0.0	0.0	0.0
28	0.041687896614016395	0.0	0.0	0.0	0.0
29	0.04245989469946115	0.0	0.0	0.0	0.0
30	0.04631988512668488	0.0	0.0	0.0	0.0
31	0.05172387172479812	0.0	0.0	0.0	0.0
32	0.055583862152021865	0.0	0.0	0.0	0.0
33	0.05635586023746661	0.0	0.0	0.0	0.0
34	0.057899856408356105	0.0	0.0	0.0	0.0
35	0.0594438525792456	0.0	0.0	0.0	0.0
36	0.06253184492102459	0.0	0.0	0.0	0.0
37	0.06253184492102459	0.0	0.0	0.0	0.0
38	0.06639183534824834	0.0	0.0	0.0	0.0
39	0.06716383343369309	0.0	0.0	0.0	0.0
40	0.06870782960458258	0.0	0.0	0.0	0.0
41	0.07025182577547208	0.0	0.0	0.0	0.0
42	0.07874380471536431	0.0	0.0	0.0	0.0
43	0.0818317970571433	0.0	0.0	0.0	0.0
44	0.08646378556981178	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCTCA	185	0.0	50.71564	41
CAAATAA	20	3.8873716E-4	50.715633	41
CTGAATT	15	0.00804135	50.576195	39
CGTAGAG	15	0.00804135	50.576195	39
GCGTAGA	15	0.0080538485	50.556343	38
AGCGTAG	15	0.008103989	50.47707	37
ACGCAAC	15	0.008103989	50.47707	37
CAGCGTA	15	0.008141746	50.417778	36
CGCTTAG	15	0.008230354	50.27997	42
TCTACAC	15	0.008255802	50.240734	43
CCGACTC	30	1.0066378E-6	50.240734	43
GCGAGTG	20	4.07223E-4	50.240734	43
TAAGCAT	20	4.0879447E-4	50.201557	46
CACGATT	50	7.2759576E-12	50.142914	47
TCGTTGA	15	0.0083324965	50.123394	14
CGTTGAT	15	0.0083324965	50.123394	15
GTGATAT	15	0.0083324965	50.123394	15
ACCTAGT	15	0.00838392	50.04547	11
GATTATG	15	0.008422643	49.98719	50
GATTACG	35	5.2508767E-8	49.987186	50
>>END_MODULE