FastQCFastQC Report
Fri 17 Jun 2016
SRR1525949_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525949_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9216
Sequences flagged as poor quality0
Sequence length40
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATGTAGGGATGAGATGGACTTAAATGAATTGACGGGTAC1431.5516493055555556No Hit
TATGTAGGGATGAGATGGACTTAAAGGAATTGACGGGTAC1411.5299479166666665No Hit
TATGTAGGGATGAGATGGACTCAAAGGAATTGACGGGTAC991.07421875No Hit
TATGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT991.07421875No Hit
TATGTAGGGATGAGATGGCCTTAAAGGAATTGACGGGTAC920.9982638888888888No Hit
TATGTAGGGATGAGATGGACTCAAATGAATTGACGGGTAC900.9765625No Hit
TATGTAGGGATGAGATGGCCTTAAATGAATTGACGGGTAC690.7486979166666667No Hit
TATGTAGGGTACCTGGTTGATCCTGCCCGTAGCATATGCT630.68359375No Hit
TATGTAGGGATGAGATGGCCTCAAATGAATTGACGGGTAC500.5425347222222222No Hit
TATGTAGGGATGAGATGGCCTCAAAGGAATTGACGGGTAC500.5425347222222222No Hit
TATGTAGGGTACCTGGTTGATCCTGCCAGTAGCCTATGCT310.3363715277777778No Hit
TATGTAGGGTACCTGGTTGATCCTGCCCGTAGCCTATGCT290.3146701388888889No Hit
TATGTAGGGATGAGATGGACTTACAGGAATTGACGGGTAC240.26041666666666663No Hit
TATGTAGGGGAGTGCAGTGCTTATGTAGGGATGAGATGGA190.20616319444444445No Hit
TATGTAGGGTACCTGGTTCATCCTGCCAGTAGCATATGCT180.1953125No Hit
TATGTAGGGATGAGATGGACTTACATGAATTGACGGGTAC160.1736111111111111No Hit
TATGTAGGGATGAGATGGCCTCCAAGGAATTGACGGGTAC150.16276041666666669No Hit
TATGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCC150.16276041666666669No Hit
TATGTAGGGATGAGATGGACTCACAGGAATTGACGGGTAC140.15190972222222224No Hit
TATGTAGGGATGAGATGGACTCACATGAATTGACGGGTAC120.13020833333333331No Hit
TATGTAGGGTACCTGGTTGATCCTGCCAGTCGCCTATGCT110.1193576388888889No Hit
TATGTAGGGTACCTGGTTGATCCTGCCAGTAGCCTCTGCT110.1193576388888889No Hit
TATGTAGGGTACCTGGTTGATCCTGCCAGTAGCATCTGCT110.1193576388888889No Hit
TATGTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT110.1193576388888889No Hit
TATGTAGGGATGAGATGGACTTAACTGAATTGACGGGTAC110.1193576388888889No Hit
TATGTAGGGATGAGATGGCCTCCAATGAATTGACGGGTAC110.1193576388888889No Hit
TATGTAGGGATGAGATGGACTTAAAGGAATTGACCGGTAC110.1193576388888889No Hit
TATGTAGGGCAGAGTGCAGTGCTTATGTAGGGATGAGATG100.10850694444444445No Hit
TATGTAGGGTACCTGGTTCATCCTGCCCGTAGCATATGCT100.10850694444444445No Hit
TATGTAGGGTACCTGGTTCATCCTGCCCGTAGCCTATGCT100.10850694444444445No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCTGC601.8189894E-1234.0710420
AGGGGCT301.3109953E-534.071046
AGGGGAG301.3109953E-534.071046
GGTTGAT601.8189894E-1234.0710415
GCCTTAA301.3109953E-534.0710418
AGGGAGT301.3109953E-534.071046
GGCCTTA301.3109953E-534.0710417
TCAAAGG301.3109953E-534.0710421
GGCCTCA301.3109953E-534.0710417
AAAGGAA601.8189894E-1234.0710423
TGGACTT601.8189894E-1234.0710416
CAAAGGA301.3109953E-534.0710422
TAGGGGG454.634785E-934.071045
TAGGGCT454.634785E-934.071045
TAGGGAG601.8189894E-1234.071045
CTCAAAG301.3109953E-534.0710420
AGGGGTG251.8528769E-434.0710376
AGGGGTA200.002620595334.0710376
GTTGATC552.3646862E-1134.07103716
GGATGAC200.002620595334.0710378
Produced by FastQC (version 0.11.5)