##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1525949_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9216
Sequences flagged as poor quality	0
Sequence length	40
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.07671440972222	30.0	26.0	33.0	4.0	34.0
2	14.87109375	15.0	8.0	20.0	4.0	26.0
3	14.651041666666666	12.0	10.0	20.0	4.0	31.0
4	19.5966796875	23.0	10.0	27.0	4.0	32.0
5	16.348524305555557	17.0	10.0	22.0	4.0	29.0
6	18.84939236111111	20.0	10.0	27.0	4.0	33.0
7	22.35492621527778	26.0	15.0	30.0	4.0	33.0
8	20.889539930555557	24.0	10.0	29.0	4.0	33.0
9	21.609266493055557	25.0	13.0	29.0	4.0	33.0
10	15.685112847222221	15.0	6.0	23.0	4.0	30.0
11	23.93500434027778	29.0	10.0	35.0	4.0	38.0
12	23.75173611111111	29.0	9.0	35.0	4.0	38.0
13	23.4560546875	28.0	6.0	34.0	4.0	37.0
14	24.632703993055557	31.0	4.0	35.0	4.0	38.0
15	21.78754340277778	26.0	4.0	33.0	4.0	36.0
16	20.706380208333332	24.0	4.0	33.0	4.0	36.0
17	21.32573784722222	26.0	4.0	33.0	4.0	36.0
18	20.587890625	25.0	4.0	33.0	4.0	36.0
19	17.78374565972222	18.0	4.0	30.0	4.0	35.0
20	19.65505642361111	23.0	4.0	33.0	4.0	36.0
21	16.8935546875	15.0	4.0	30.0	4.0	35.0
22	16.98773871527778	14.0	4.0	30.0	4.0	35.0
23	16.17339409722222	13.0	4.0	29.0	4.0	34.0
24	16.17990451388889	12.0	4.0	30.0	4.0	34.0
25	15.298285590277779	4.0	4.0	28.0	4.0	34.0
26	14.02322048611111	4.0	4.0	27.0	4.0	34.0
27	13.840169270833334	4.0	4.0	27.0	4.0	34.0
28	13.00802951388889	4.0	4.0	25.0	4.0	33.0
29	12.673828125	4.0	4.0	24.0	4.0	33.0
30	12.006510416666666	4.0	4.0	22.0	4.0	32.0
31	11.51953125	4.0	4.0	22.0	4.0	32.0
32	10.341905381944445	4.0	4.0	17.0	4.0	30.0
33	10.36056857638889	4.0	4.0	17.0	4.0	30.0
34	10.681640625	4.0	4.0	17.0	4.0	32.0
35	9.6142578125	4.0	4.0	10.0	4.0	29.0
36	9.75368923611111	4.0	4.0	4.0	4.0	31.0
37	9.774739583333334	4.0	4.0	4.0	4.0	32.0
38	9.612847222222221	4.0	4.0	4.0	4.0	31.0
39	9.305989583333334	4.0	4.0	4.0	4.0	31.0
40	9.740559895833334	4.0	4.0	4.0	4.0	32.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	-14.593429228141916
1	2	-11.176728100257
1	3	-11.173545485504562
1	4	-15.365253239480364
1	5	-12.217237378917922
1	6	-13.221305699369918
1	7	-17.21744702457309
1	8	-15.675898207749945
1	9	-16.209942437069746
1	10	-10.247232500536365
1	11	-17.820003691179124
1	12	-17.715356586398464
1	13	-16.891703288907056
1	14	-18.349818203633244
1	15	-15.489072342136893
1	16	-15.252108651915186
1	17	-15.853163576643478
1	18	-14.94677725814406
1	19	-12.547919273691413
1	20	-14.314149021676144
1	21	-12.235964411851832
1	22	-11.996301864170453
1	23	-11.375056903735684
1	24	-11.251249740086617
1	25	-10.824795014796518
1	26	-9.480977258327572
1	27	-9.197789836031436
1	28	-8.412368167880913
1	29	-8.160845104695609
1	30	-7.598553046440605
1	31	-7.5310786209096445
1	32	-6.46543482758049
1	33	-6.534421582768346
1	34	-6.749925670759174
1	35	-5.519370859174966
1	36	-5.874485816984054
1	37	-5.823439999292805
1	38	-5.705910301720367
1	39	-5.171888040393224
1	40	-5.548206130521663
2	1	6.493527293597214
2	2	3.823271899743
2	3	-11.173545485504562
2	4	-15.365253239480364
2	5	-12.217237378917922
2	6	-13.221305699369918
2	7	-17.21744702457309
2	8	-15.675898207749945
2	9	-16.209942437069746
2	10	-10.247232500536365
2	11	-17.820003691179124
2	12	-17.715356586398464
2	13	-16.891703288907056
2	14	-18.349818203633244
2	15	-15.489072342136893
2	16	-15.252108651915186
2	17	-15.853163576643478
2	18	-14.94677725814406
2	19	-12.547919273691413
2	20	-14.314149021676144
2	21	-12.235964411851832
2	22	-11.996301864170453
2	23	-11.375056903735684
2	24	-11.251249740086617
2	25	-10.824795014796518
2	26	-9.480977258327572
2	27	-9.197789836031436
2	28	-8.412368167880913
2	29	-8.160845104695609
2	30	-7.598553046440605
2	31	-7.5310786209096445
2	32	-6.46543482758049
2	33	-6.534421582768346
2	34	-6.749925670759174
2	35	-5.519370859174966
2	36	-5.874485816984054
2	37	-5.823439999292805
2	38	-5.705910301720367
2	39	-5.171888040393224
2	40	-5.548206130521663
3	1	4.993527293597214
3	2	10.323271899743
3	3	21.32645451449544
3	4	1.6347467605196364
3	5	4.782762621082078
3	6	-13.221305699369918
3	7	-17.21744702457309
3	8	-15.675898207749945
3	9	-16.209942437069746
3	10	-10.247232500536365
3	11	-17.820003691179124
3	12	-17.715356586398464
3	13	-16.891703288907056
3	14	-18.349818203633244
3	15	-15.489072342136893
3	16	-15.252108651915186
3	17	-15.853163576643478
3	18	-14.94677725814406
3	19	-12.547919273691413
3	20	-14.314149021676144
3	21	-12.235964411851832
3	22	-11.996301864170453
3	23	-11.375056903735684
3	24	-11.251249740086617
3	25	-10.824795014796518
3	26	-9.480977258327572
3	27	-9.197789836031436
3	28	-8.412368167880913
3	29	-8.160845104695609
3	30	-7.598553046440605
3	31	-7.5310786209096445
3	32	-6.46543482758049
3	33	-6.534421582768346
3	34	-6.749925670759174
3	35	-5.519370859174966
3	36	-5.874485816984054
3	37	-5.823439999292805
3	38	-5.705910301720367
3	39	-5.171888040393224
3	40	-5.548206130521663
5	1	0.49352729359721437
5	2	6.323271899743
5	3	1.3264545144954383
5	4	-10.03191990614703
5	5	-3.5505707122512558
5	6	1.2786943006300824
5	7	4.115886308760242
5	8	5.9907684589167225
5	9	-2.8766091037364134
5	10	-0.5805658338696986
5	11	3.5133296421542077
5	12	4.117976746934868
5	13	5.274963377759612
5	14	5.983515129700088
5	15	5.677594324529775
5	16	4.2478913480848135
5	17	5.146836423356522
5	18	6.219889408522608
5	19	5.452080726308587
5	20	5.019184311657188
5	21	11.0973689214815
5	22	10.170364802496215
5	23	11.458276429597648
5	24	12.748750259913383
5	25	14.34187165187015
5	26	11.35235607500576
5	27	9.468876830635232
5	28	10.754298498785754
5	29	-0.32751177136227483
5	30	2.901446953559395
5	31	-3.1977452875763106
5	32	-1.2987681609138235
5	33	-0.7010882494350117
5	34	0.2500743292408263
5	35	0.31396247415836775
5	36	1.9588475163492802
5	37	0.8432266673738606
5	38	0.2940896982796328
5	39	1.16144529294011
5	40	-1.0482061305216632
6	1	1.793527293597215
6	2	4.923271899743002
6	3	-4.373545485504561
6	4	-6.365253239480364
6	5	7.082762621082079
6	6	5.578694300630083
6	7	5.182552975426908
6	8	5.224101792250053
6	9	6.690057562930253
6	10	9.452767499463635
6	11	9.279996308820877
6	12	9.384643413601538
6	13	13.108296711092944
6	14	10.750181796366757
6	15	8.410927657863105
6	16	-0.05210865191518721
6	17	7.646836423356522
6	18	10.453222741855939
6	19	7.352080726308586
6	20	6.085850978323855
6	21	15.164035588148167
6	22	11.503698135829547
6	23	16.324943096264313
6	24	17.748750259913383
6	25	16.275204985203484
6	26	12.519022741672428
6	27	6.702210163968562
6	28	6.387631832119087
6	29	9.139154895304392
6	30	4.601446953559394
6	31	4.768921379090356
6	32	5.834565172419511
6	33	3.1655784172316537
6	34	4.650074329240827
6	35	3.5806291408250335
6	36	1.8255141830159456
6	37	2.576560000707195
6	38	4.5940896982796335
6	39	4.528111959606775
6	40	1.5517938694783364
7	1	5.185834985904908
7	2	8.938656515127617
7	3	1.0572237452646682
7	4	3.980900606673483
7	5	8.628916467235925
7	6	10.432540454476236
7	7	7.936399129273063
7	8	8.247178715326978
7	9	7.751596024468714
7	10	10.291229037925175
7	11	8.679996308820876
7	12	7.669258798216919
7	13	9.108296711092944
7	14	7.765566411751372
7	15	9.93400458094003
7	16	8.440199040392507
7	17	10.569913346433445
7	18	5.32245351108671
7	19	7.221311495539357
7	20	10.724312516785396
7	21	11.340958665071245
7	22	12.349851981983393
7	23	11.740327711648932
7	24	13.210288721451843
7	25	11.982897292895789
7	26	10.519022741672428
7	27	8.956056317814717
7	28	11.664554909042163
7	29	10.031462587612085
7	30	8.593754645867088
7	31	5.391998302167279
7	32	3.842257480111819
7	33	4.042501494154731
7	34	4.442382021548518
7	35	-0.5962939360980428
7	36	-0.2591012015994387
7	37	-0.3234399992928054
7	38	-0.05206414787421387
7	39	-0.40265727116245387
7	40	0.18256310024756672
8	1	-3.1731393730694535
8	2	-0.6767281002569998
8	3	-4.340212152171228
8	4	-4.1985865728136975
8	5	-3.717237378917922
8	6	1.0286943006300824
8	7	0.2825529754269098
8	8	2.157435125583387
8	9	-0.04327577040307773
8	10	-4.413899167203031
8	11	5.096662975487543
8	12	-0.9653565863984639
8	13	3.2749633777596117
8	14	3.4835151297000877
8	15	-1.3224056754702254
8	16	1.7478913480848135
8	17	-4.436496909976812
8	18	2.719889408522608
8	19	1.5354140596419192
8	20	4.269184311657188
8	21	5.264035588148168
8	22	5.503698135829547
8	23	9.124943096264316
8	24	5.582083593246715
8	25	-0.9081283481298517
8	26	6.35235607500576
8	27	3.7188768306352316
8	28	1.8376318321190865
8	29	2.3391548953043912
8	30	2.734780286892729
8	31	2.8022547124236894
8	32	1.3678985057528443
8	33	1.6322450838983205
8	34	4.33340766257416
8	35	1.8972958074917
8	36	3.5421808496826124
8	37	1.2598933340405285
8	38	4.294089698279633
8	39	4.16144529294011
8	40	5.368460536145003
9	1	7.493527293597214
9	2	7.823271899743
9	3	-3.1735454855045617
9	4	10.134746760519636
9	5	7.782762621082078
9	6	12.778694300630082
9	7	13.28255297542691
9	8	14.824101792250055
9	9	14.290057562930254
9	10	12.752767499463635
9	11	13.679996308820876
9	12	13.784643413601536
9	13	3.108296711092944
9	14	3.6501817963667555
9	15	10.510927657863107
9	16	16.247891348084814
9	17	14.646836423356522
9	18	15.55322274185594
9	19	-2.547919273691413
9	20	13.185850978323856
9	21	15.264035588148168
9	22	14.503698135829547
9	23	5.624943096264316
9	24	14.748750259913383
9	25	16.175204985203482
9	26	12.519022741672428
9	27	11.802210163968564
9	28	9.587631832119087
9	29	5.339154895304391
9	30	3.901446953559395
9	31	-5.0310786209096445
9	32	1.5345651724195104
9	33	-0.5344215827683456
9	34	5.750074329240826
9	35	-0.01937085917496617
9	36	5.125514183015946
9	37	2.6765600007071946
9	38	8.794089698279633
9	39	-5.171888040393224
9	40	-5.548206130521663
10	1	10.177737819913002
10	2	8.560114005006158
10	3	18.14224398817965
10	4	14.740009918414376
10	5	12.835394200029448
10	6	18.46290482694587
10	7	14.83518455437428
10	8	15.692522844881637
10	9	14.36900493135131
10	10	16.96329381525311
10	11	10.232627887768246
10	12	12.705696045180481
10	13	13.371454605829786
10	14	12.808076533208858
10	15	8.247769763126264
10	16	11.484733453347971
10	17	11.988941686514416
10	18	12.05322274185594
10	19	12.136291252624378
10	20	8.422693083587014
10	21	10.079825061832377
10	22	13.372119188461125
10	23	7.993364148895894
10	24	7.275066049387068
10	25	9.069941827308746
10	26	3.519022741672428
10	27	4.1706312166001425
10	28	2.6402634110664547
10	29	5.20757594793597
10	30	4.190920637769921
10	31	5.9426055896166705
10	32	2.7450914882089847
10	33	6.833999469863233
10	34	5.671126960819775
10	35	5.849050193456613
10	36	3.3360404988054206
10	37	3.0712968428124583
10	38	2.346721277227001
10	39	4.933375117501512
10	40	3.7675833431625474
12	1	4.564955865025787
12	2	7.0375576140287155
12	3	2.8264545144954383
12	4	8.634746760519636
12	5	7.068476906796363
12	6	8.278694300630082
12	7	7.711124403998337
12	8	11.60981607796434
12	9	10.432914705787397
12	10	9.46705321374935
12	11	12.822853451678021
12	12	13.784643413601536
12	13	10.03686813966437
12	14	11.293038939223901
12	15	10.939499086434534
12	16	14.747891348084814
12	17	13.861122137642237
12	18	13.267508456141655
12	19	8.59493786916573
12	20	14.185850978323856
12	21	13.835464159576741
12	22	13.07512670725812
12	23	13.339228810550031
12	24	16.463035974199098
12	25	13.318062128060625
12	26	6.590451313101001
12	27	3.7307815925399908
12	28	8.230488974976229
12	29	8.053440609590107
12	30	9.544304096416537
12	31	4.8974928076617825
12	32	4.53456517241951
12	33	6.17986413151737
12	34	5.678645757812253
12	35	2.4092005693964627
12	36	2.9112284687302328
12	37	4.247988572135766
12	38	4.579803983993919
12	39	1.9709691024639184
12	40	6.237508155192623
13	1	-3.1428363427664223
13	2	-6.8130917366206365
13	3	-2.7190000309591067
13	4	-10.456162330389455
13	5	-8.308146469827012
13	6	-9.130396608460826
13	7	-9.035628842754909
13	8	-8.1304436622954
13	9	-9.6644878916152
13	10	-5.429050682354546
13	11	-6.910912782088216
13	12	-5.988083859125737
13	13	-8.25533965254342
13	14	-6.986181839996881
13	15	-7.943617796682348
13	16	-4.433926833733368
13	17	-4.853163576643478
13	18	-4.310413621780423
13	19	-8.27519200096414
13	20	-3.1323308398579623
13	21	-0.5996007754881951
13	22	-1.087210955079545
13	23	-1.102329631008411
13	24	0.11238662354974593
13	25	0.7206595306580272
13	26	-0.39006816741848027
13	27	1.620391982150382
13	28	2.2239954684827232
13	29	1.2027912589407546
13	30	-0.598553046440605
13	31	-4.985624075455099
13	32	-4.192707554853217
13	33	-4.352603400950164
13	34	-4.749925670759174
13	35	-3.2466435864476937
13	36	-2.783576726074963
13	37	-3.005258181110987
13	38	-2.6150012108112763
13	39	-2.080978949484133
13	40	-2.730024312339845
14	1	6.493527293597214
14	2	1.8232718997430002
14	3	14.326454514495438
14	4	9.634746760519636
14	5	4.782762621082078
14	6	11.278694300630082
14	7	12.78255297542691
14	8	13.324101792250055
14	9	12.790057562930254
14	10	11.252767499463635
14	11	10.679996308820876
14	12	7.784643413601536
14	13	7.608296711092944
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112	37	0.7321155562627499
112	38	-0.37257696838703325
112	39	1.4947786262734422
112	40	-0.2148727971883293
114	1	7.32686062693055
114	2	11.489938566409668
114	3	14.65978784782877
114	4	12.634746760519636
114	5	11.949429287748746
114	6	10.778694300630082
114	7	8.449219642093578
114	8	10.824101792250055
114	9	6.123390896263587
114	10	10.752767499463635
114	11	6.513329642154208
114	12	5.784643413601536
114	13	-0.7250366222403883
114	14	2.3168484630334234
114	15	8.010927657863107
114	16	10.247891348084814
114	17	6.31350309002319
114	18	10.219889408522608
114	19	4.452080726308587
114	20	3.519184311657188
114	21	6.430702254814836
114	22	0.17036480249621455
114	23	-2.2083902370690183
114	24	3.5820835932467148
114	25	4.175204985203482
114	26	-1.147643924994238
114	27	0.3022101639685637
114	28	-3.5790348345475795
114	29	-2.4941784380289427
114	30	0.06811362022606104
114	31	-3.1977452875763106
114	32	-3.2987681609138226
114	33	-5.034421582768346
114	34	-1.9165923374258398
114	35	-5.519370859174966
114	36	-5.874485816984054
114	37	-5.823439999292805
114	38	-5.705910301720367
114	39	-5.171888040393224
114	40	-5.548206130521663
116	1	-6.506472706402786
116	2	2.823271899743
116	3	-0.6735454855045617
116	4	-0.8652532394803636
116	5	0.7827626210820782
116	6	-1.7213056993699176
116	7	-2.71744702457309
116	8	0.32410179225005464
116	9	-0.20994243706974558
116	10	-7.247232500536365
116	11	-2.3200036911791244
116	12	-3.215356586398464
116	13	-5.891703288907056
116	14	-2.8498182036332445
116	15	-12.489072342136893
116	16	-2.2521086519151865
116	17	-3.853163576643478
116	18	-1.9467772581440599
116	19	-11.547919273691413
116	20	-1.314149021676144
116	21	2.264035588148168
116	22	2.5036981358295467
116	23	-11.375056903735684
116	24	-11.251249740086617
116	25	-10.824795014796518
116	26	-9.480977258327572
116	27	-9.197789836031436
116	28	-8.412368167880913
116	29	-8.160845104695609
116	30	-7.598553046440605
116	31	-7.5310786209096445
116	32	-6.46543482758049
116	33	-6.534421582768346
116	34	-6.749925670759174
116	35	-5.519370859174966
116	36	-5.874485816984054
116	37	-5.823439999292805
116	38	-5.705910301720367
116	39	-5.171888040393224
116	40	-5.548206130521663
117	1	9.493527293597214
117	2	12.323271899743
117	3	16.32645451449544
117	4	8.134746760519636
117	5	11.282762621082078
117	6	14.278694300630082
117	7	14.28255297542691
117	8	5.824101792250055
117	9	10.790057562930254
117	10	19.752767499463637
117	11	8.179996308820876
117	12	6.284643413601536
117	13	4.108296711092944
117	14	9.150181796366756
117	15	8.010927657863107
117	16	-3.7521086519151865
117	17	-4.353163576643478
117	18	-0.9467772581440599
117	19	1.952080726308587
117	20	0.685850978323856
117	21	1.2640355881481682
117	22	-8.996301864170453
117	23	2.1249430962643157
117	24	-8.251249740086617
117	25	2.6752049852034823
117	26	-6.480977258327572
117	27	-0.6977898360314363
117	28	0.5876318321190865
117	29	-4.160845104695609
117	30	3.401446953559395
117	31	6.9689213790903555
117	32	8.03456517241951
117	33	7.965578417231654
117	34	4.750074329240826
117	35	7.480629140825034
117	36	11.125514183015946
117	37	11.176560000707195
117	38	11.294089698279633
117	39	11.828111959606776
117	40	11.451793869478337
118	1	6.493527293597214
118	2	9.823271899743
118	3	2.8264545144954383
118	4	12.634746760519636
118	5	-12.217237378917922
118	6	-13.221305699369918
118	7	-17.21744702457309
118	8	-15.675898207749945
118	9	-16.209942437069746
118	10	-10.247232500536365
118	11	-17.820003691179124
118	12	-17.715356586398464
118	13	-16.891703288907056
118	14	-18.349818203633244
118	15	-15.489072342136893
118	16	-15.252108651915186
118	17	-15.853163576643478
118	18	-14.94677725814406
118	19	-12.547919273691413
118	20	-14.314149021676144
118	21	-12.235964411851832
118	22	-11.996301864170453
118	23	-11.375056903735684
118	24	-11.251249740086617
118	25	-10.824795014796518
118	26	-9.480977258327572
118	27	-9.197789836031436
118	28	-8.412368167880913
118	29	-8.160845104695609
118	30	-7.598553046440605
118	31	-7.5310786209096445
118	32	-6.46543482758049
118	33	-6.534421582768346
118	34	-6.749925670759174
118	35	-5.519370859174966
118	36	-5.874485816984054
118	37	-5.823439999292805
118	38	-5.705910301720367
118	39	-5.171888040393224
118	40	-5.548206130521663
119	1	9.743527293597214
119	2	12.823271899743
119	3	20.07645451449544
119	4	6.884746760519636
119	5	9.032762621082078
119	6	4.778694300630082
119	7	1.0325529754269098
119	8	5.574101792250055
119	9	3.0400575629302544
119	10	-4.997232500536365
119	11	-6.320003691179124
119	12	-11.465356586398464
119	13	-5.641703288907056
119	14	-13.099818203633244
119	15	-0.9890723421368932
119	16	-8.752108651915186
119	17	-15.853163576643478
119	18	-14.94677725814406
119	19	-12.547919273691413
119	20	-14.314149021676144
119	21	-12.235964411851832
119	22	-11.996301864170453
119	23	-11.375056903735684
119	24	-11.251249740086617
119	25	-10.824795014796518
119	26	-9.480977258327572
119	27	-9.197789836031436
119	28	-8.412368167880913
119	29	-8.160845104695609
119	30	-7.598553046440605
119	31	-7.5310786209096445
119	32	-6.46543482758049
119	33	-6.534421582768346
119	34	-6.749925670759174
119	35	-5.519370859174966
119	36	-5.874485816984054
119	37	-5.823439999292805
119	38	-5.705910301720367
119	39	-5.171888040393224
119	40	-5.548206130521663
120	1	10.693527293597217
120	2	15.023271899743
120	3	21.02645451449544
120	4	14.234746760519638
120	5	17.38276262108208
120	6	9.378694300630084
120	7	-5.4174470245730895
120	8	7.724101792250053
120	9	1.5900575629302551
120	10	-4.847232500536364
120	11	-11.620003691179125
120	12	-14.915356586398463
120	13	-13.891703288907056
120	14	-12.149818203633245
120	15	-10.089072342136893
120	16	-13.852108651915186
120	17	-15.253163576643479
120	18	-10.54677725814406
120	19	-7.147919273691413
120	20	-10.714149021676144
120	21	-11.235964411851832
120	22	-11.796301864170452
120	23	-5.575056903735684
120	24	-8.651249740086618
120	25	-8.224795014796518
120	26	-3.6809772583275713
120	27	-9.197789836031436
120	28	-8.412368167880913
120	29	-8.160845104695609
120	30	-7.598553046440605
120	31	-7.5310786209096445
120	32	-6.46543482758049
120	33	-6.534421582768346
120	34	-6.749925670759174
120	35	-5.519370859174966
120	36	-5.874485816984054
120	37	-5.823439999292805
120	38	-5.705910301720367
120	39	-5.171888040393224
120	40	-5.548206130521663
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
4	1921.0
5	95.0
6	36.0
7	61.0
8	38.0
9	102.0
10	183.0
11	273.0
12	321.0
13	340.0
14	366.0
15	399.0
16	378.0
17	370.0
18	371.0
19	427.0
20	394.0
21	389.0
22	387.0
23	426.0
24	368.0
25	375.0
26	322.0
27	263.0
28	244.0
29	140.0
30	100.0
31	68.0
32	34.0
33	13.0
34	10.0
35	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	0.0	100.0	0.0
4	100.0	0.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	100.0	0.0	0.0
7	98.40321529437324	0.5974364544862046	0.8364110362806866	0.162937214859874
8	97.87187839305102	0.7274701411509229	0.6840390879478827	0.7166123778501629
9	97.50217202432667	1.0860121633362294	0.5321459600347525	0.8796698523023458
10	27.03201302224634	47.61801410743353	14.910472056429736	10.439500813890396
11	20.25824652777778	28.16840277777778	39.04079861111111	12.532552083333334
12	49.97829861111111	12.543402777777779	15.190972222222221	22.28732638888889
13	18.9453125	37.25043402777778	19.075520833333336	24.72873263888889
14	40.73350694444444	19.25998263888889	24.75043402777778	15.25607638888889
15	24.72873263888889	39.43142361111111	19.921875	15.91796875
16	25.911458333333332	15.266927083333334	42.48046875	16.341145833333336
17	41.86197916666667	15.8203125	24.06684027777778	18.250868055555554
18	40.55989583333333	18.326822916666664	23.057725694444446	18.055555555555554
19	20.881392818280737	26.996735582154518	13.460282916213275	38.66158868335147
20	17.762586805555554	23.61111111111111	18.663194444444446	39.96310763888889
21	16.65581597222222	16.276041666666664	47.65625	19.41189236111111
22	14.583333333333334	15.50564236111111	30.41449652777778	39.49652777777778
23	18.782552083333336	36.11111111111111	20.29079861111111	24.815538194444446
24	15.800325556158437	35.38795442213782	26.5979381443299	22.213781877373847
25	23.94044772875462	38.15474896761574	18.180830254292545	19.7239730493371
26	26.18813503769256	16.78138315306457	31.58527258822244	25.44520922102043
27	35.988712828304756	16.648578250488388	20.338615150857393	27.024093770349467
28	15.514059276951473	39.88709152100749	21.995440234502226	22.60340896753881
29	20.454545454545457	35.036972596781204	23.07525010874293	21.433231839930407
30	14.416276792514415	14.56859971711457	50.636492220650645	20.37863126972038
31	14.596712746271907	21.20387504081855	47.4257102427343	16.773701970175246
32	40.937703494682005	14.510527458215758	26.96982852181463	17.581940525287607
33	13.25954861111111	33.36588541666667	27.137586805555557	26.236979166666668
34	12.28298611111111	19.80251736111111	29.56814236111111	38.34635416666667
35	30.54048187540699	16.160191013674844	35.0662036032125	18.233123507705663
36	31.018016062513563	22.43325374430215	28.934230518775777	17.61449967440851
37	29.199695949614508	16.397002931914432	37.57194049299598	16.831360625475078
38	18.51128472222222	16.9921875	47.93836805555556	16.55815972222222
39	12.447097124253933	34.053174172544765	28.35594139989148	25.14378730330982
40	11.50173611111111	20.008680555555554	33.63715277777778	34.85243055555556
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	1.0
8	1.0
9	2.0
10	3.0
11	7.0
12	11.0
13	11.0
14	19.0
15	27.0
16	31.5
17	36.0
18	36.0
19	54.5
20	73.0
21	98.5
22	124.0
23	124.0
24	126.5
25	129.0
26	151.0
27	173.0
28	173.0
29	194.5
30	216.0
31	255.0
32	294.0
33	294.0
34	330.5
35	367.0
36	393.5
37	420.0
38	420.0
39	575.5
40	731.0
41	863.0
42	995.0
43	995.0
44	1031.5
45	1068.0
46	1037.5
47	1007.0
48	1007.0
49	925.5
50	844.0
51	757.5
52	671.0
53	671.0
54	613.5
55	556.0
56	497.5
57	439.0
58	439.0
59	389.5
60	340.0
61	298.0
62	256.0
63	256.0
64	222.5
65	189.0
66	157.5
67	126.0
68	126.0
69	93.0
70	60.0
71	49.5
72	39.0
73	39.0
74	26.5
75	14.0
76	9.0
77	4.0
78	4.0
79	3.0
80	2.0
81	1.5
82	1.0
83	1.0
84	0.5
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.10850694444444445
8	0.06510416666666666
9	0.08680555555555555
10	0.010850694444444444
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.2821180555555555
20	0.0
21	0.0
22	0.0
23	0.0
24	0.010850694444444444
25	0.15190972222222224
26	0.68359375
27	0.021701388888888888
28	0.054253472222222224
29	0.2170138888888889
30	0.2712673611111111
31	0.3146701388888889
32	0.021701388888888888
33	0.0
34	0.0
35	0.021701388888888888
36	0.021701388888888888
37	0.07595486111111112
38	0.0
39	0.010850694444444444
40	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
40	9216.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.09982638888889
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.59496071525332	75.77039930555556
2	3.2105120563532914	5.143229166666666
3	0.8669737198591168	2.083333333333333
4	0.3522080736927662	1.128472222222222
5	0.20319696559198047	0.8138020833333334
6	0.1219181793551883	0.5859375
7	0.135464643727987	0.759548611111111
8	0.0541858574911948	0.3472222222222222
9	0.0541858574911948	0.390625
>10	0.2980222162015714	4.340277777777778
>50	0.0812787862367922	5.555555555555555
>100	0.0270929287455974	3.0815972222222223
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATGTAGGGATGAGATGGACTTAAATGAATTGACGGGTAC	143	1.5516493055555556	No Hit
TATGTAGGGATGAGATGGACTTAAAGGAATTGACGGGTAC	141	1.5299479166666665	No Hit
TATGTAGGGATGAGATGGACTCAAAGGAATTGACGGGTAC	99	1.07421875	No Hit
TATGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT	99	1.07421875	No Hit
TATGTAGGGATGAGATGGCCTTAAAGGAATTGACGGGTAC	92	0.9982638888888888	No Hit
TATGTAGGGATGAGATGGACTCAAATGAATTGACGGGTAC	90	0.9765625	No Hit
TATGTAGGGATGAGATGGCCTTAAATGAATTGACGGGTAC	69	0.7486979166666667	No Hit
TATGTAGGGTACCTGGTTGATCCTGCCCGTAGCATATGCT	63	0.68359375	No Hit
TATGTAGGGATGAGATGGCCTCAAATGAATTGACGGGTAC	50	0.5425347222222222	No Hit
TATGTAGGGATGAGATGGCCTCAAAGGAATTGACGGGTAC	50	0.5425347222222222	No Hit
TATGTAGGGTACCTGGTTGATCCTGCCAGTAGCCTATGCT	31	0.3363715277777778	No Hit
TATGTAGGGTACCTGGTTGATCCTGCCCGTAGCCTATGCT	29	0.3146701388888889	No Hit
TATGTAGGGATGAGATGGACTTACAGGAATTGACGGGTAC	24	0.26041666666666663	No Hit
TATGTAGGGGAGTGCAGTGCTTATGTAGGGATGAGATGGA	19	0.20616319444444445	No Hit
TATGTAGGGTACCTGGTTCATCCTGCCAGTAGCATATGCT	18	0.1953125	No Hit
TATGTAGGGATGAGATGGACTTACATGAATTGACGGGTAC	16	0.1736111111111111	No Hit
TATGTAGGGATGAGATGGCCTCCAAGGAATTGACGGGTAC	15	0.16276041666666669	No Hit
TATGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCC	15	0.16276041666666669	No Hit
TATGTAGGGATGAGATGGACTCACAGGAATTGACGGGTAC	14	0.15190972222222224	No Hit
TATGTAGGGATGAGATGGACTCACATGAATTGACGGGTAC	12	0.13020833333333331	No Hit
TATGTAGGGTACCTGGTTGATCCTGCCAGTCGCCTATGCT	11	0.1193576388888889	No Hit
TATGTAGGGTACCTGGTTGATCCTGCCAGTAGCCTCTGCT	11	0.1193576388888889	No Hit
TATGTAGGGTACCTGGTTGATCCTGCCAGTAGCATCTGCT	11	0.1193576388888889	No Hit
TATGTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT	11	0.1193576388888889	No Hit
TATGTAGGGATGAGATGGACTTAACTGAATTGACGGGTAC	11	0.1193576388888889	No Hit
TATGTAGGGATGAGATGGCCTCCAATGAATTGACGGGTAC	11	0.1193576388888889	No Hit
TATGTAGGGATGAGATGGACTTAAAGGAATTGACCGGTAC	11	0.1193576388888889	No Hit
TATGTAGGGCAGAGTGCAGTGCTTATGTAGGGATGAGATG	10	0.10850694444444445	No Hit
TATGTAGGGTACCTGGTTCATCCTGCCCGTAGCATATGCT	10	0.10850694444444445	No Hit
TATGTAGGGTACCTGGTTCATCCTGCCCGTAGCCTATGCT	10	0.10850694444444445	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.010850694444444444	0.0	0.0	0.0	0.0
28	0.010850694444444444	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCCTGC	60	1.8189894E-12	34.07104	20
AGGGGCT	30	1.3109953E-5	34.07104	6
AGGGGAG	30	1.3109953E-5	34.07104	6
GGTTGAT	60	1.8189894E-12	34.07104	15
GCCTTAA	30	1.3109953E-5	34.07104	18
AGGGAGT	30	1.3109953E-5	34.07104	6
GGCCTTA	30	1.3109953E-5	34.07104	17
TCAAAGG	30	1.3109953E-5	34.07104	21
GGCCTCA	30	1.3109953E-5	34.07104	17
AAAGGAA	60	1.8189894E-12	34.07104	23
TGGACTT	60	1.8189894E-12	34.07104	16
CAAAGGA	30	1.3109953E-5	34.07104	22
TAGGGGG	45	4.634785E-9	34.07104	5
TAGGGCT	45	4.634785E-9	34.07104	5
TAGGGAG	60	1.8189894E-12	34.07104	5
CTCAAAG	30	1.3109953E-5	34.07104	20
AGGGGTG	25	1.8528769E-4	34.071037	6
AGGGGTA	20	0.0026205953	34.071037	6
GTTGATC	55	2.3646862E-11	34.071037	16
GGATGAC	20	0.0026205953	34.071037	8
>>END_MODULE