FastQCFastQC Report
Fri 17 Jun 2016
SRR1525948_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525948_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2201
Sequences flagged as poor quality0
Sequence length40
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATGTAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT100.4543389368468878No Hit
TATGTAGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT90.408905043162199No Hit
TATGTAGGGCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.2271694684234439No Hit
TATGTAGGGGGGCTGGTGCGATGGCCCAGTGGGTAAGAGC50.2271694684234439No Hit
TATGTAGGGGGGCTGGTGAGATGGCCCAGTGGGTAAGAGC50.2271694684234439No Hit
TATGTAGGGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.2271694684234439No Hit
TATGTAGGGGCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.1817355747387551No Hit
TATGTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT40.1817355747387551No Hit
TATGTAGGGTTCGACTTTTTTTTTTTTTTTTTTTTTTTTT30.13630168105406634No Hit
TATGTAGGGACCTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.13630168105406634No Hit
TATGTAGGGGGGCTGGTGAGATGGCCCCGTGGGTAAGAGC30.13630168105406634No Hit
TATGTAGGGTCCCTGGTTGATCCTCCCAGTACCATATGCT30.13630168105406634No Hit
TATGTAGGGAACGACTTTTTTTTTTTTTTTTTTTTTTTTT30.13630168105406634No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG402.9232979E-834.6279076
GTAGGGC200.00202651334.6279074
GTAGGGA650.034.6279074
TAGGGGG600.034.6279075
TAGGGAT251.2873083E-434.6279075
TATGTAG2050.033.7833251
ATGTAGG2050.033.7833252
TGTAGGG1950.033.7400133
GTAGGGG1000.032.896514
Produced by FastQC (version 0.11.5)