FastQCFastQC Report
Fri 17 Jun 2016
SRR1525947_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525947_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6927
Sequences flagged as poor quality0
Sequence length40
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT3094.4608055435253355No Hit
TATGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC510.7362494586401039No Hit
TATGTAGGGTAACTGGTTGATCCTGCCAGTAGCATATGCT370.534141764111448No Hit
TATGTAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT370.534141764111448No Hit
TATGTAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG270.3897791251624079No Hit
TATGTAGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT200.28872527789808No Hit
TATGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCC150.21654395842356No Hit
TATGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG140.20210769452865596No Hit
TATGTAGGGTACATGGTTGATCCTGCCAGTAGCATATGCT130.187671430633752No Hit
TATGTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130.187671430633752No Hit
TATGTAGGGGAACGTCTGCCCTATCAACTTTCGATGGTAG130.187671430633752No Hit
TATGTAGGGCTCCTACTTGGATAACTGTGGTAATTCTAGA110.158798902843944No Hit
TATGTAGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG110.158798902843944No Hit
TATGTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110.158798902843944No Hit
TATGTAGGGTACCTGGTTGACCCTGCCAGTAGCATATGCT80.11549011115923198No Hit
TATGTAGGGAGTTATGGTTCCTTTGGTCGCTCGCTCCTCT80.11549011115923198No Hit
TATGTAGGGGGGGAGGTAGTGACGAAAAATAACAATACAG80.11549011115923198No Hit
TATGTAGGGTACCTGGTTCATCCTGCCAGTAGCATATGCT80.11549011115923198No Hit
TATGTAGGGTACCTGGTTGATCCTGCCAGAAGCATATGCT70.10105384726432798No Hit
TATGTAGGGTACCTGGTTGATCCTGCCAGTACCATATGCT70.10105384726432798No Hit
TATGTAGGGTACCTGGTTAATCCTGCCAGTAGCATATGCT70.10105384726432798No Hit
TATGTAGGGTACCTGGTTGATACTGCCAGTAGCATATGCT70.10105384726432798No Hit
TATGTAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT70.10105384726432798No Hit
TATGTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG70.10105384726432798No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGTAG453.9053702E-934.37526
GCCAGTA453.9053702E-934.37525
AGTAGCA502.6739144E-1034.37499628
CAGTAGC502.6739144E-1034.37499627
GTAGCAT502.6739144E-1034.37499629
CCTGCCA552.0008883E-1134.12409222
AGGGGTT251.8669624E-433.8768126
AGGGGTG251.8669624E-433.8768126
ATCCTCT200.002648892633.87681216
TCCTCTT200.002648892633.87681217
GTTGATC454.5274646E-933.87681216
AGGGGGT251.8669624E-433.8768126
AGGGGGG359.23832E-733.8768126
AGGGGGC406.4772394E-833.8768126
AGGGGGA503.1468517E-1033.8768126
TGGTTGA503.1468517E-1033.87681214
AGGGGAT200.002648892633.8768126
GGTTGAT454.5274646E-933.87681215
GTACCTG359.23832E-733.8768129
GGGTACC359.23832E-733.8768127
Produced by FastQC (version 0.11.5)