SRR1525946_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1525946_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	27515
Sequences flagged as poor quality	0
Sequence length	56
%GC	54

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GGTACAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT	367	1.3338179174995457	No Hit
GGTACAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC	266	0.9667454115936762	No Hit
GGTACAGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT	195	0.7087043430855896	No Hit
GGTACAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	144	0.5233508995093585	No Hit
GGTACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	114	0.41431946211157555	No Hit
GGTACAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC	101	0.3670725059058695	No Hit
GGTACAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT	83	0.3016536434671997	No Hit
GGTACAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	81	0.29438488097401416	No Hit
GGTACAGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC	75	0.27257859349445757	No Hit
GGTACAGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT	69	0.25077230601490097	No Hit
GGTACAGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC	67	0.24350354352171544	No Hit
GGTACAGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC	62	0.2253316372887516	No Hit
GGTACAGGGGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAA	54	0.19625658731600945	No Hit
GGTACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTC	50	0.18171906232963836	No Hit
GGTACAGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC	48	0.17445029983645283	No Hit
GGTACAGGGGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAG	47	0.17081591858986006	No Hit
GGTACAGGGGTCAAAGCCTAGGGGTCCCTGGGTTGGCAGGCTGCTCTGGGGGGTCT	44	0.15991277485008176	No Hit
GGTACAGGGGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGC	44	0.15991277485008176	No Hit
GGTACAGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATG	42	0.15264401235689623	No Hit
GGTACAGGGGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCAT	41	0.14900963111030346	No Hit
GGTACAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA	39	0.14174086861711793	No Hit
GGTACAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG	38	0.13810648737052517	No Hit
GGTACAGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA	37	0.1344721061239324	No Hit
GGTACAGGGAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTG	37	0.1344721061239324	No Hit
GGTACAGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCC	35	0.12720334363074687	No Hit
GGTACAGGGCTCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCG	32	0.11630019989096856	No Hit
GGTACAGGGGGTCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGGCTGCTCTTCCA	32	0.11630019989096856	No Hit
GGTACAGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTCATGGTTCCTTTGGTC	29	0.10539705615119024	No Hit
GGTACAGGGGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCC	29	0.10539705615119024	No Hit
GGTACAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCCGATTGAACAAAAACTC	29	0.10539705615119024	No Hit
GGTACAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT	29	0.10539705615119024	No Hit
GGTACAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC	28	0.10176267490459749	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTCCAA	15	0.0076936735	51.033646	38
GTCCAAT	15	0.0076936735	51.033646	39
TCCAATC	15	0.0076936735	51.033646	40
CGATTGA	15	0.0076936735	51.033646	40
ATCCATT	20	3.8200337E-4	50.749077	37
TTGTCCA	15	0.007866122	50.749073	37
TTTTGTC	15	0.007924239	50.65492	35
AAGAGCC	15	0.007924239	50.65492	35
AAGAGAC	15	0.007924239	50.65492	35
AGAGCCC	15	0.007924239	50.65492	36
TTTGTCC	15	0.007924239	50.65492	36
CGACACA	20	3.9627845E-4	50.374542	44
TAAGAGA	20	3.9627845E-4	50.374542	34
GACACAA	15	0.008100508	50.37454	45
TAATCCG	15	0.008100508	50.37454	42
ATTGACC	15	0.008100508	50.37454	42
TATTAAA	15	0.008100508	50.37454	34
AGTTAAA	20	4.0357318E-4	50.189342	49
AGTGGTA	15	0.00834005	50.005497	14
GAGCCAA	15	0.00834005	50.005497	9

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content