##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1525946_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	27515
Sequences flagged as poor quality	0
Sequence length	56
%GC	54
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.99563874250409	38.0	33.0	39.0	29.0	40.0
2	34.965255315282576	38.0	34.0	39.0	27.0	40.0
3	34.474650190805015	38.0	33.0	39.0	26.0	40.0
4	32.152644012356895	38.0	29.0	39.0	15.0	40.0
5	34.25240777757587	38.0	33.0	39.0	25.0	40.0
6	31.300636016718155	38.0	25.0	39.0	12.0	40.0
7	34.31706341995275	38.0	33.0	39.0	24.0	40.0
8	34.474650190805015	38.0	33.0	39.0	26.0	40.0
9	34.49067781210249	38.0	33.0	39.0	25.0	40.0
10	33.812865709612936	38.0	33.0	39.0	22.0	40.0
11	34.21275667817554	38.0	33.0	39.0	24.0	40.0
12	32.88620752316918	37.0	31.0	39.0	18.0	40.0
13	32.25338906051245	36.0	29.0	39.0	17.0	40.0
14	32.93483554424859	38.0	31.0	39.0	19.0	40.0
15	31.917027076140286	36.0	29.0	39.0	15.0	40.0
16	32.55751408322733	37.0	30.0	39.0	19.0	40.0
17	31.655169907323277	36.0	28.0	38.0	15.0	40.0
18	30.96990732327821	35.0	26.0	38.0	14.0	40.0
19	32.22326003997819	36.0	29.0	38.0	18.0	40.0
20	32.90870434308559	37.0	30.0	39.0	22.0	40.0
21	33.22885698709795	38.0	31.0	39.0	19.0	40.0
22	32.57892058876976	37.0	30.0	39.0	17.0	40.0
23	33.140432491368344	38.0	31.0	39.0	19.0	40.0
24	32.49958204615664	37.0	30.0	39.0	17.0	40.0
25	33.023986916227514	37.0	31.0	39.0	20.0	40.0
26	33.42653098310013	38.0	33.0	39.0	19.0	40.0
27	33.69049609304016	38.0	33.0	39.0	22.0	40.0
28	33.76296565509722	38.0	33.0	39.0	23.0	40.0
29	33.28177357804834	38.0	32.0	39.0	19.0	40.0
30	33.00890423405415	38.0	32.0	39.0	19.0	40.0
31	33.718190078139195	38.0	33.0	39.0	22.0	40.0
32	33.63401780846811	38.0	33.0	39.0	22.0	40.0
33	33.42496819916409	38.0	33.0	39.0	21.0	40.0
34	33.31844448482646	38.0	33.0	39.0	20.0	40.0
35	33.20283481737234	38.0	33.0	39.0	19.0	40.0
36	32.66305651462839	37.0	32.0	39.0	18.0	40.0
37	32.43310921315646	36.0	31.0	38.0	16.0	40.0
38	31.707068871524623	36.0	30.0	38.0	10.0	40.0
39	30.633145556968927	35.0	28.0	38.0	4.0	40.0
40	28.524295838633474	34.0	22.0	38.0	4.0	39.0
41	26.623986916227512	32.0	17.0	38.0	4.0	39.0
42	27.431982554970016	32.0	21.0	38.0	4.0	39.0
43	27.649863710703254	33.0	22.0	38.0	4.0	39.0
44	26.730692349627475	32.0	18.0	37.0	4.0	39.0
45	27.6115209885517	33.0	23.0	38.0	4.0	39.0
46	25.609849173178265	30.0	17.0	36.0	4.0	39.0
47	24.74377612211521	29.0	12.0	36.0	4.0	39.0
48	25.23390877703071	30.0	15.0	36.0	4.0	39.0
49	24.228166454661093	29.0	10.0	36.0	4.0	39.0
50	24.950572415046338	30.0	14.0	36.0	4.0	39.0
51	25.184226785389786	30.0	10.0	36.0	4.0	39.0
52	24.908667999273124	30.0	4.0	36.0	4.0	39.0
53	24.824386698164638	30.0	4.0	36.0	4.0	39.0
54	24.37779393058332	30.0	4.0	36.0	4.0	39.0
55	24.10219880065419	30.0	4.0	36.0	4.0	39.0
56	23.128838815191713	29.0	4.0	36.0	4.0	38.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	5.5681900703113385
1	2	5.279971434266606
1	3	5.234215858344754
1	4	8.843737925689695
1	5	5.4549804457316
1	6	10.338112509907635
1	7	6.288281780316339
1	8	6.046742595399529
1	9	6.18614003686001
1	10	6.772737212770984
1	11	1.11338202021917
1	12	-6.569080880968517
1	13	-19.53930185299432
1	14	-6.282562088283829
1	15	2.0356994698869038
1	16	-2.54559321626542
1	17	-7.876995982284544
1	18	-6.072741217743353
1	19	-0.5282282718850659
1	20	-1.0418421103965088
1	21	6.603887394383943
1	22	7.2230470353519
1	23	6.438189371091145
1	24	7.244620016001466
1	25	6.5758800463524345
1	26	6.746246854387504
1	27	5.909188788924567
1	28	5.480060540168665
1	29	6.031806744787829
1	30	6.545308465586981
1	31	4.978264409905439
1	32	5.1683017261141835
1	33	4.69366985411094
1	34	5.415009636541022
1	35	5.55788422790193
1	36	3.4098223675310244
1	37	2.8941691809030985
1	38	3.871954788222329
1	39	4.663362486590451
1	40	-13.274742175859176
1	41	-3.315719685625613
1	42	-3.602198400943699
1	43	-12.115885537369012
1	44	-15.552288747046319
1	45	-5.231980346871527
1	46	-7.165182840858474
1	47	-10.854910840539313
1	48	-6.4537082380453406
1	49	-14.537336138560704
1	50	-6.541324265012223
1	51	-1.0172691188414582
1	52	-0.8299736920102703
1	53	-0.5194189158608573
1	54	-1.0959836419969058
1	55	-1.0099571266535747
1	56	2.950964859758894
2	1	5.942103113789599
2	2	5.491927956005739
2	3	5.1613897713882295
2	4	9.275259664820133
2	5	6.097371750079425
2	6	10.198982075125027
2	7	6.6067600411859
2	8	6.393481725834313
2	9	6.287226993381751
2	10	5.627085038857935
2	11	4.147077672393081
2	12	1.7080930320749594
2	13	2.241132929614377
2	14	6.288090085629218
2	15	6.169395122060816
2	16	4.381580696778055
2	17	6.928438800324152
2	18	4.63921530399578
2	19	6.228293467245372
2	20	5.171201367864359
2	21	6.738670003079594
2	22	6.577394861438851
2	23	6.759928501525923
2	24	7.663098276871029
2	25	6.776967002874176
2	26	6.0201598978657636
2	27	5.924406180228921
2	28	5.3267996706034495
2	29	5.763328483918265
2	30	6.5398736829782855
2	31	4.628264409905437
2	32	5.721562595679405
2	33	5.073017680197896
2	34	4.250879201758416
2	35	4.632884227901933
2	36	5.006561497965809
2	37	5.772430050468316
2	38	5.404563483874501
2	39	6.226405964851317
2	40	5.358953476314738
2	41	7.954932488287433
2	42	7.153236381664996
2	43	6.956940549587511
2	44	-3.1348974426984952
2	45	7.307150087911083
2	46	5.596773680880656
2	47	2.84291524641721
2	48	5.186509153259006
2	49	-2.5112491820389664
2	50	5.553240952379081
2	51	8.681643924636802
2	52	8.114591525381034
2	53	8.992537605878272
2	54	8.860538097133528
2	55	8.2226515689986
2	56	10.26835616410672
3	1	6.1348567369780085
3	2	5.660526989822159
3	3	5.198104747233643
3	4	9.040960147911917
3	5	6.3022026679538214
3	6	10.738112509907634
3	7	6.618837335871895
3	8	6.449520373177307
3	9	6.583362259082229
3	10	6.900514990548757
3	11	5.805048686885833
3	12	6.211474674587041
3	13	6.410698147005679
3	14	6.18132680060506
3	15	1.327366136553568
3	16	6.618295672623464
3	17	7.820226239937682
3	18	8.530036560034421
3	19	6.427327283670493
3	20	5.9692690007146005
3	21	7.192776283272831
3	22	6.90638036868523
3	23	7.393744926646701
3	24	7.364064460445913
3	25	6.206435601907987
3	26	6.2240246321652855
3	27	5.403633233369014
3	28	5.438393873502001
3	29	6.206806744787826
3	30	6.014752910031422
3	31	4.63937552101655
3	32	3.376635059447519
3	33	3.13533652077761
3	34	4.023342969874356
3	35	3.6328842279019327
3	36	5.773711256419919
3	37	4.660835847569764
3	38	5.044177010444553
3	39	6.613362486590447
3	40	7.728035601918599
3	41	10.20372475881883
3	42	8.411690487945187
3	43	8.086892240408762
3	44	8.514377919620348
3	45	8.33468631979514
3	46	10.187594936919304
3	47	10.906200270571798
3	48	9.91018065084355
3	49	10.651552750328182
3	50	9.861453512765554
3	51	9.752175325602987
3	52	9.189470752434172
3	53	10.105581084139146
3	54	10.115127469114206
3	55	10.506709540013095
3	56	9.28429819309223
4	1	5.179301181422453
4	2	4.860526989822162
4	3	4.731438080566974
4	4	8.92984903680081
4	5	5.5466471123982615
4	6	10.115890287685414
4	7	6.1966151136496705
4	8	6.27174259539953
4	9	6.005584481304453
4	10	5.856070546104313
4	11	5.73838202021917
4	12	7.255919119031486
4	13	7.0551425914501245
4	14	7.3368823561606185
4	15	8.194032803220235
4	16	7.529406783734576
4	17	7.998004017715459
4	18	9.174481004478867
4	19	7.805105061448266
4	20	6.502602334047939
4	21	7.03722072771728
4	22	7.261935924240785
4	23	6.660411593313366
4	24	8.186286682668136
4	25	7.584213379685767
4	26	5.601802409943058
4	27	6.2925221222579
4	28	5.349504984613112
4	29	5.962362300343386
4	30	6.414752910031428
4	31	5.63937552101655
4	32	4.909968392780851
4	33	5.024225409666496
4	34	5.334454080985466
4	35	4.7662175612352655
4	36	5.9070445897532515
4	37	5.438613625347543
4	38	6.355288121555663
4	39	6.324473597701562
4	40	2.661368935251936
4	41	11.292613647707718
4	42	10.478357154611857
4	43	9.95355890707543
4	44	11.314377919620345
4	45	10.379130764239584
4	46	12.654261603585972
4	47	13.883978048349572
4	48	12.732402873065773
4	49	11.58488608366152
4	50	12.972564623876668
4	51	12.263286436714093
4	52	11.767248530211955
4	53	11.750025528583585
4	54	11.826238580225318
4	55	13.306709540013092
4	56	10.306520415314452
5	1	5.780385192262564
5	2	5.222044604998317
5	3	4.631167077856951
5	4	8.76995743788482
5	5	5.684858494512085
5	6	10.157624705029587
5	7	6.307184219340726
5	8	6.261986497838556
5	9	5.961140036860009
5	10	5.956883554234395
5	11	5.416430800706969
5	12	7.202260582446119
5	13	7.85460058603007
5	14	6.287559862935684
5	15	8.32899215281373
5	16	8.38794336910043
5	17	9.115077188447167
5	18	8.918112440793234
5	19	8.222991240310058
5	20	7.453279840823004
5	21	7.986814223652235
5	22	7.431583620717749
5	23	7.067457663774068
5	24	8.412302942830731
5	25	7.28014833903536
5	26	6.334051732436283
5	27	5.627481471851397
5	28	6.085548345046718
5	29	6.806806744787828
5	30	6.926406026562592
5	31	5.201435141612752
5	32	4.779887091967847
5	33	4.53696253703777
5	34	5.050985246297124
5	35	4.193859837658032
5	36	5.289700416311515
5	37	4.233193571146998
5	38	5.4268328370028165
5	39	7.2523868768343505
5	40	8.872209043653022
5	41	9.076353485106097
5	42	9.595972330763615
5	43	9.231065682143186
5	44	9.476979545636606
5	45	8.872897701908961
5	46	10.717134232312262
5	47	11.312162330192397
5	48	11.15787712780832
5	49	10.77241995900027
5	50	10.366602564256066
5	51	10.678462588475615
5	52	9.915758015306803
5	53	10.881190840236705
5	54	10.989382211661631
5	55	10.342481897736668
5	56	10.111940469514995
6	1	5.387237689358962
6	2	5.857352386647555
6	3	5.7949301440590375
6	4	8.842547449499222
6	5	6.475218540969692
6	6	10.914302986098111
6	7	5.682329399363958
6	8	5.824123547780481
6	9	5.794473370193344
6	10	6.27511816515193
6	11	5.347905829742977
6	12	6.674966738079103
6	13	7.086888623196156
6	14	6.04481886409712
6	15	7.513080422267851
6	16	6.396073450401243
6	17	7.536099255810697
6	18	8.528449258447122
6	19	7.271771728114935
6	20	5.901015032460638
6	21	5.922935013431566
6	22	6.796856559161423
6	23	6.2619988949006675
6	24	6.952953349334802
6	25	5.66516576063815
6	26	5.727199235339885
6	27	4.954426884162665
6	28	5.186012921121048
6	29	5.902044840025923
6	30	5.908403703682222
6	31	5.223502505143532
6	32	4.5052064880189455
6	33	4.1972412826823735
6	34	5.08524773177912
6	35	4.942408037425743
6	36	5.461012843721502
6	37	6.056073942807856
6	38	5.907669073936614
6	39	7.146695819923785
6	40	7.27406734795035
6	41	11.11642317151724
6	42	10.570420646675352
6	43	11.109114462630988
6	44	11.33818744342987
6	45	9.520400605509423
6	46	11.843150492474862
6	47	12.471279635651161
6	48	11.787958428621323
6	49	12.400759099534536
6	50	11.112247163559207
6	51	11.81725469068235
6	52	11.030740593704014
6	53	11.262723941281997
6	54	11.808778262765
6	55	10.868614301917855
6	56	11.765250574044611
7	1	6.149142451263721
7	2	5.357352386647555
7	3	5.699692048820943
7	4	9.437785544737316
7	5	5.618075683826838
7	6	10.700017271812396
7	7	5.610900827935389
7	8	6.252694976351911
7	9	6.223044798764768
7	10	5.989403879437646
7	11	6.014572496409642
7	12	7.2940143571267235
7	13	7.634507670815207
7	14	7.497199816478076
7	15	7.965461374648807
7	16	7.8246448789726735
7	17	8.631337351048792
7	18	9.099877829875698
7	19	7.6051050614482705
7	20	7.353395984841587
7	21	7.58960168009823
7	22	7.868285130589992
7	23	6.976284609186383
7	24	7.810096206477656
7	25	7.831832427304814
7	26	6.20338971153036
7	27	6.216331646067424
7	28	5.519346254454383
7	29	5.925854363835448
7	30	6.217927513206032
7	31	4.913978695619722
7	32	4.743301726114186
7	33	4.221050806491892
7	34	4.20429535082674
7	35	4.394788989806692
7	36	4.556250938959597
7	37	6.008454895188812
7	38	5.883859550127088
7	39	7.3609815342095
7	40	7.059781633664635
7	41	9.449756504850576
7	42	10.59423017048487
7	43	9.442447795964323
7	44	9.64771125295368
7	45	8.806114891223714
7	46	9.86696001628438
7	47	11.280803445174971
7	48	11.383196523859418
7	49	11.115044813820244
7	50	9.088437639749682
7	51	9.079159452587113
7	52	9.268835831799255
7	53	9.857962036520096
7	54	10.165921119907857
7	55	10.178138111441662
7	56	8.9557267645208
8	1	5.902336411774755
8	2	5.173264117193433
8	3	4.679947565661827
8	4	9.160201340323844
8	5	6.0751023969511095
8	6	10.279575924541785
8	7	5.819379341291949
8	8	6.237596253936118
8	9	6.205042475884397
8	10	6.225176237161229
8	11	6.074967386072828
8	12	6.348602045860748
8	13	7.049722537249583
8	14	7.189998887325924
8	15	8.207040933301538
8	16	7.778187271539455
8	17	7.895564993325209
8	18	8.74738073347616
8	19	7.710796118358836
8	20	6.794743255457153
8	21	7.0112044675546805
8	22	7.577925084132385
8	23	7.506482054017969
8	24	7.095229772099028
8	25	7.792343460986583
8	26	5.968198073899703
8	27	4.920164398680669
8	28	5.427011759680866
8	29	6.0507091838122165
8	30	5.2678694411967335
8	31	4.030703434295685
8	32	3.755496848065409
8	33	4.293060098013381
8	34	3.6851315877605373
8	35	4.43776227668242
8	36	5.484822367531027
8	37	5.160022839439684
8	38	5.28049137358818
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116	34	5.021259636541025
116	35	5.539134227901933
116	36	6.734822367531027
116	37	6.075419180903097
116	38	6.934454788222329
116	39	6.750862486590449
116	40	9.125257824140824
116	41	7.7905303143743865
116	42	7.460301599056301
116	43	3.5153644626309877
116	44	2.5539612529536804
116	45	7.974269653128474
116	46	2.6973171591415266
116	47	-5.5049108405393135
116	48	2.6525417619546587
116	49	0.35016386143929523
116	50	1.6836757349877765
116	51	7.03898088115854
116	52	6.401276307989729
116	53	5.780581084139143
116	54	5.966516358003094
116	55	6.002542873346425
116	56	9.582214859758896
117	1	3.851523403644677
117	2	3.5299714342666064
117	3	3.3425491916780885
117	4	6.943737925689696
117	5	4.213313779064933
117	6	7.479779176574301
117	7	4.563281780316338
117	8	4.538409262066196
117	9	5.169473370193344
117	10	3.6977372127709813
117	11	-0.21161797978083285
117	12	-8.694080880968517
117	13	-9.639301852994318
117	14	-1.9908954216171608
117	15	-6.1059671967797655
117	16	-8.853926549598754
117	17	-3.1186626489512115
117	18	-4.614407884410021
117	19	-2.3115616052183974
117	20	-1.741842110396508
117	21	2.262220727717281
117	22	2.606380368685233
117	23	3.738189371091142
117	24	3.119620016001466
117	25	3.53421337968577
117	26	3.0962468543875055
117	27	4.275855455591234
117	28	3.3467272068353324
117	29	3.973473411454492
117	30	4.836975132253645
117	31	2.7532644099054373
117	32	3.201635059447522
117	33	4.102003187444279
117	34	3.6983429698743606
117	35	2.7578842279019327
117	36	5.318155700864363
117	37	4.919169180903097
117	38	5.413621454888993
117	39	6.146695819923785
117	40	8.916924490807489
117	41	7.050946981041054
117	42	4.231134932389633
117	43	-3.5158855373690123
117	44	2.1477112529536804
117	45	4.859686319795141
117	46	0.42648382580819444
117	47	0.37008915946068655
117	48	0.4546250952879909
117	49	-2.670669471894037
117	50	2.4753424016544443
117	51	6.549397547825208
117	52	5.995026307989729
117	53	5.447247750805811
117	54	7.737349691336426
117	55	7.815042873346425
117	56	11.134298193092228
118	1	4.398624852920037
118	2	3.6875801299187785
118	3	3.2048680322577994
118	4	6.862216186559259
118	5	4.575632619644644
118	6	8.633764683820683
118	7	5.019803519446775
118	8	4.567394769312578
118	9	4.178531341207837
118	10	5.279258951901419
118	11	1.0601211506539485
118	12	-6.509298272272865
118	13	-9.780606200820404
118	14	-9.472779479588176
118	15	-6.591474443156574
118	16	-10.835810607569769
118	17	-0.028082938806281987
118	18	-6.056436869917267
118	19	-0.38040218492854194
118	20	-1.6983638495269417
118	21	3.825626524818727
118	22	3.9904383396997254
118	23	3.3686241536998374
118	24	3.8587504507840755
118	25	3.798706133308958
118	26	4.194072941344029
118	27	3.6200583541419604
118	28	4.652886627125191
118	29	3.198111092613914
118	30	4.387699769934809
118	31	2.7369600620793513
118	32	4.069388682635928
118	33	2.833887245415294
118	34	4.229140071323634
118	35	3.2415798800758466
118	36	4.57177888927016
118	37	5.402864833077011
118	38	4.1002156577875475
118	39	5.9872755300687075
118	40	8.358953476314738
118	41	5.802758575243953
118	42	5.522801599056301
118	43	-2.064798580847274
118	44	0.7346677746928094
118	45	5.198454435737169
118	46	-5.772791536510649
118	47	0.21248046380851093
118	48	-3.4221864989149076
118	49	-3.272118747256357
118	50	-2.446759047620919
118	51	2.5512091420281067
118	52	2.831982829728858
118	53	1.775146301530448
118	54	3.012712010177008
118	55	2.8313472211725106
118	56	4.333573555411071
119	1	5.228190070311342
119	2	4.864971434266607
119	3	5.349215858344756
119	4	8.698737925689699
119	5	5.719980445731601
119	6	9.898112509907637
119	7	5.7832817803163366
119	8	5.711742595399535
119	9	5.841140036860011
119	10	6.082737212770979
119	11	2.538382020219167
119	12	-8.504080880968516
119	13	-1.909301852994318
119	14	-3.147562088283827
119	15	-9.059300530113099
119	16	-7.390593216265422
119	17	0.9580040177154565
119	18	-2.727741217743354
119	19	0.6317717281149342
119	20	-0.5818421103965079
119	21	5.623887394383946
119	22	6.493047035351896
119	23	5.67818937109114
119	24	5.279620016001463
119	25	5.850880046352433
119	26	6.166246854387506
119	27	5.399188788924569
119	28	5.945060540168669
119	29	6.526806744787827
119	30	6.450308465586982
119	31	4.128264409905437
119	32	5.643301726114185
119	33	5.228669854110944
119	34	5.810009636541025
119	35	5.472884227901936
119	36	7.00482236753103
119	37	7.354169180903099
119	38	8.141954788222332
119	39	8.09336248659045
119	40	10.750257824140824
119	41	9.719280314374387
119	42	8.5228015990563
119	43	-2.0508855373690125
119	44	4.607711252953681
119	45	9.188019653128471
119	46	1.6898171591415263
119	47	0.3950891594606851
119	48	-0.5787082380453406
119	49	-0.5573361385607036
119	50	4.923675734987775
119	51	10.147730881158541
119	52	10.585026307989732
119	53	10.02558108413914
119	54	9.964016358003093
119	55	11.120042873346424
119	56	12.550964859758896
120	1	5.161047213168487
120	2	4.797828577123752
120	3	4.652073001201899
120	4	8.42588078283255
120	5	5.058551874303028
120	6	9.759541081336206
120	7	5.170424637459192
120	8	5.514599738256678
120	9	5.639711465431439
120	10	5.929880069913835
120	11	1.8955248773620212
120	12	-5.658366595254233
120	13	-5.103587567280034
120	14	-4.193276373998113
120	15	1.3106994698869023
120	16	-8.520593216265421
120	17	0.405146874858314
120	18	-4.5548840748862105
120	19	-0.5496568433136382
120	20	-0.3846992532536504
120	21	3.982458822955376
120	22	4.7730470353518974
120	23	4.523903656805427
120	24	4.47676287314432
120	25	3.879451474923865
120	26	6.167675425816075
120	27	5.752045931781716
120	28	5.6264891115972375
120	29	5.556806744787828
120	30	6.527451322729835
120	31	5.592550124191153
120	32	4.886158868971332
120	33	5.4472412826823735
120	34	5.347152493683879
120	35	4.632884227901933
120	36	4.270536653245312
120	37	4.222740609474528
120	38	4.229097645365183
120	39	5.313362486590449
120	40	5.321686395569397
120	41	-5.597862542768471
120	42	-5.548626972372272
120	43	-10.962314108797584
120	44	-15.530860175617748
120	45	-4.931980346871526
120	46	-9.133039983715616
120	47	-8.719196554825027
120	48	-6.914422523759628
120	49	-15.230193281417847
120	50	-6.0663242650122235
120	51	-1.3494119759843173
120	52	-2.004973692010271
120	53	-0.6301332015751413
120	54	-1.5959836419969058
120	55	-0.6670999837964331
120	56	1.0866791454731803
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	952.0
5	32.0
6	22.0
7	44.0
8	33.0
9	45.0
10	54.0
11	53.0
12	48.0
13	51.0
14	53.0
15	103.0
16	148.0
17	155.0
18	221.0
19	254.0
20	372.0
21	533.0
22	569.0
23	682.0
24	780.0
25	864.0
26	901.0
27	863.0
28	935.0
29	1057.0
30	1185.0
31	1220.0
32	1359.0
33	1594.0
34	1792.0
35	1901.0
36	2035.0
37	2448.0
38	2221.0
39	1936.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	100.0	0.0	0.0	0.0
3	0.0	0.0	100.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	0.0	100.0
6	0.0	100.0	0.0	0.0
7	99.32400508813375	0.421588224604761	0.19625658731600945	0.05815009994548428
8	99.50935853170998	0.23623478102852988	0.20352534980919498	0.050881337452298746
9	99.18226421951663	0.3670725059058695	0.27621297474105033	0.17445029983645283
10	56.77630383427221	19.247683081955298	15.089950935853173	8.886062147919317
11	38.975104488460836	21.606396510994003	16.07123387243322	23.34726512811194
12	33.09104125022715	21.4573868798837	18.73886970743231	26.712702162456843
13	25.335271669998182	13.727057968380882	21.551880792295112	39.38578956932582
14	22.591313828820645	29.30401599127748	23.61257495911321	24.49209522078866
15	33.357661569901104	18.563660913038717	23.86600007298471	24.212677444075467
16	30.66739407164939	27.212220403709765	22.14220767412257	19.978177850518275
17	28.370469993820652	18.77067354876231	32.99044018756134	19.868416269855693
18	24.128657096129384	19.585680537888425	24.80828638924223	31.477375976739957
19	26.662787737735915	22.088075930033817	20.040728753772864	31.208407578457397
20	27.724609730359155	21.90240529820603	29.87882537025581	20.494159601178996
21	25.75873223567041	21.727910442336352	21.88783484171119	30.625522480282047
22	21.108486280210794	20.203525349809194	34.45756859894603	24.230419771033983
23	22.04979102307832	21.7590405233509	31.891695438851535	24.299473014719243
24	22.66482517990841	20.484117176710036	24.30035618230719	32.55070146107436
25	25.383496910214465	22.540894220283533	29.16030534351145	22.91530352599055
26	34.162484544330496	21.776129173030768	20.746963415521126	23.31442286711761
27	20.81847944707166	28.621316842488177	21.66606038559476	28.894143324845402
28	26.321145598604346	26.361125245329653	21.803445518645052	25.51428363742095
29	32.081103709366246	25.419533326052928	18.765243347530124	23.73411961705071
30	31.344097752563822	21.121536111717216	26.812859117026694	20.721507018692268
31	32.715376226826606	25.419847328244277	22.210105416212286	19.65467102871683
32	33.80471135669623	24.785516940526392	21.182928602588337	20.226843100189036
33	33.42066004439108	24.74256813302769	21.096677946366846	20.740093876214388
34	24.278277330809274	22.847573628453894	33.57603116240126	19.298117878335578
35	24.01718112987769	23.434769947582993	34.052853814793245	18.495195107746067
36	27.998981855205262	25.213628595323804	26.511763208610596	20.27562634086033
37	30.0414967967385	22.553873034362258	26.506988934187536	20.897641234711706
38	30.355777284148427	26.557663595644733	23.7318378791741	19.35472124103274
39	30.108505680163123	23.314156714244103	20.18278473638217	26.394552869210603
40	23.726575965381937	19.05827129707727	24.368330338479595	32.8468223990612
41	21.049344159900063	16.19208582871	21.59312194584267	41.16544806554727
42	22.031921871583705	28.201297281539244	21.56912761460535	28.1976532322717
43	19.032869324393264	22.625901902193718	28.977479775526565	29.363748997886454
44	23.147404541140396	23.65116448857414	25.337665182156677	27.863765788128784
45	26.883012820512818	21.947843822843822	26.635343822843822	24.533799533799534
46	21.69728783902012	27.781423155438905	23.140857392825897	27.38043161271508
47	18.212368046170145	23.81195894363882	24.356211418343864	33.61946159184717
48	25.501109534722982	21.783258758048675	25.730292116846737	26.985339590381606
49	21.077180963486626	23.241746228409006	23.700896436119816	31.980176371984548
50	22.83289627039627	25.247668997669	25.44798951048951	26.471445221445222
51	21.8225594630089	24.2776886035313	27.155990077338394	26.743761856121406
52	21.236617871968537	28.934527711018866	26.13793605709708	23.690918359915518
53	22.227082194277383	27.475852013850922	26.429743028977583	23.867322762894112
54	22.320192377759966	21.91576185965168	29.836770385484222	25.92727537710413
55	19.080962800875273	22.88110867979577	30.503282275711157	27.5346462436178
56	20.912769292457988	21.466846498742388	30.106076623045237	27.514307585754384
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	2.0
11	4.0
12	14.5
13	34.0
14	43.0
15	41.5
16	40.0
17	36.0
18	32.0
19	35.5
20	39.0
21	45.0
22	45.0
23	39.0
24	46.5
25	54.0
26	64.5
27	75.0
28	80.0
29	105.0
30	125.0
31	131.0
32	137.0
33	188.0
34	239.0
35	276.0
36	313.0
37	340.0
38	390.0
39	413.0
40	468.0
41	523.0
42	572.0
43	621.0
44	873.0
45	1125.0
46	1630.0
47	2172.0
48	2209.0
49	1946.5
50	1684.0
51	1643.0
52	1602.0
53	1526.0
54	1612.5
55	1775.0
56	1736.0
57	1697.0
58	1759.5
59	1822.0
60	1958.0
61	2094.0
62	1884.5
63	1519.0
64	1363.0
65	1283.5
66	1204.0
67	965.0
68	726.0
69	635.5
70	545.0
71	456.0
72	363.0
73	359.0
74	259.0
75	159.0
76	117.5
77	76.0
78	64.5
79	87.5
80	122.0
81	81.0
82	40.0
83	23.0
84	6.0
85	4.0
86	2.0
87	1.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.40705069961838997
16	0.07268762493185535
17	0.01453752498637107
18	0.0
19	0.05815009994548428
20	0.1235689623841541
21	0.007268762493185535
22	0.0
23	0.0
24	0.0036343812465927674
25	0.018171906232963837
26	0.06178448119207705
27	0.09085953116481918
28	0.0036343812465927674
29	0.15991277485008176
30	0.06178448119207705
31	0.018171906232963837
32	0.025440668726149373
33	0.11630019989096856
34	0.16718153734326732
35	0.156278393603489
36	0.050881337452298746
37	0.156278393603489
38	0.19625658731600945
39	0.18535344357623115
40	0.8940577866618208
41	1.0830456114846447
42	0.26530983100127203
43	0.26530983100127203
44	0.4397601308377249
45	0.21442849354897328
46	0.3016536434671997
47	0.5015446120298019
48	0.09449391241141196
49	0.26530983100127203
50	0.21442849354897328
51	0.37434126839905507
52	0.19262220606941666
53	0.2907504997274214
54	0.25077230601490097
55	0.3452662184263129
56	0.29801926222060693
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
56	27515.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.7063419952753
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.52002027369488	63.47446847174268
2	6.614292954891029	9.485735053607124
3	1.9310694374049668	4.154097764855534
4	0.94272681196148	2.7039796474650193
5	0.5068423720223011	1.8171906232963837
6	0.3395843892549417	1.4610212611302926
7	0.16218955904713633	0.8141013992367799
8	0.1824632539280284	1.046701799018717
9	0.10643689812468322	0.6868980556060331
>10	0.6284845413076533	8.257314192258768
>50	0.035478966041561075	1.784481192077049
>100	0.030410542321338063	4.314010539705615
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTACAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT	367	1.3338179174995457	No Hit
GGTACAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC	266	0.9667454115936762	No Hit
GGTACAGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT	195	0.7087043430855896	No Hit
GGTACAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	144	0.5233508995093585	No Hit
GGTACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	114	0.41431946211157555	No Hit
GGTACAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC	101	0.3670725059058695	No Hit
GGTACAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT	83	0.3016536434671997	No Hit
GGTACAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	81	0.29438488097401416	No Hit
GGTACAGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC	75	0.27257859349445757	No Hit
GGTACAGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT	69	0.25077230601490097	No Hit
GGTACAGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC	67	0.24350354352171544	No Hit
GGTACAGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC	62	0.2253316372887516	No Hit
GGTACAGGGGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAA	54	0.19625658731600945	No Hit
GGTACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTC	50	0.18171906232963836	No Hit
GGTACAGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC	48	0.17445029983645283	No Hit
GGTACAGGGGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAG	47	0.17081591858986006	No Hit
GGTACAGGGGTCAAAGCCTAGGGGTCCCTGGGTTGGCAGGCTGCTCTGGGGGGTCT	44	0.15991277485008176	No Hit
GGTACAGGGGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGC	44	0.15991277485008176	No Hit
GGTACAGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATG	42	0.15264401235689623	No Hit
GGTACAGGGGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCAT	41	0.14900963111030346	No Hit
GGTACAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA	39	0.14174086861711793	No Hit
GGTACAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG	38	0.13810648737052517	No Hit
GGTACAGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA	37	0.1344721061239324	No Hit
GGTACAGGGAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTG	37	0.1344721061239324	No Hit
GGTACAGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCC	35	0.12720334363074687	No Hit
GGTACAGGGCTCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCG	32	0.11630019989096856	No Hit
GGTACAGGGGGTCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGGCTGCTCTTCCA	32	0.11630019989096856	No Hit
GGTACAGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTCATGGTTCCTTTGGTC	29	0.10539705615119024	No Hit
GGTACAGGGGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCC	29	0.10539705615119024	No Hit
GGTACAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCCGATTGAACAAAAACTC	29	0.10539705615119024	No Hit
GGTACAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT	29	0.10539705615119024	No Hit
GGTACAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC	28	0.10176267490459749	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0036343812465927674	0.0	0.0	0.0	0.0
12	0.0036343812465927674	0.0	0.0	0.0	0.0
13	0.0036343812465927674	0.0	0.0	0.0	0.0
14	0.01453752498637107	0.0	0.0	0.0	0.0
15	0.01453752498637107	0.0	0.0	0.0	0.0
16	0.018171906232963837	0.0	0.0	0.0	0.0
17	0.018171906232963837	0.0	0.0	0.0	0.0
18	0.018171906232963837	0.0	0.0	0.0	0.0
19	0.018171906232963837	0.0	0.0	0.0	0.0
20	0.018171906232963837	0.0	0.0	0.0	0.0
21	0.021806287479556607	0.0	0.0	0.0	0.0
22	0.021806287479556607	0.0	0.0	0.0	0.0
23	0.021806287479556607	0.0	0.0	0.0	0.0
24	0.025440668726149373	0.0	0.0	0.0	0.0
25	0.025440668726149373	0.0	0.0	0.0	0.0
26	0.03270943121933491	0.0	0.0	0.0	0.0
27	0.03997819371252044	0.0	0.0	0.0	0.0
28	0.03997819371252044	0.0	0.0	0.0	0.0
29	0.04724695620570598	0.0	0.0	0.0	0.0
30	0.04724695620570598	0.0	0.0	0.0	0.0
31	0.05451571869889151	0.0	0.0	0.0	0.0
32	0.05451571869889151	0.0	0.0	0.0	0.0
33	0.05451571869889151	0.0	0.0	0.0	0.0
34	0.05451571869889151	0.0	0.0	0.0	0.0
35	0.05451571869889151	0.0	0.0	0.0	0.0
36	0.05815009994548428	0.0	0.0	0.0	0.0
37	0.05815009994548428	0.0	0.0	0.0	0.0
38	0.05815009994548428	0.0	0.0	0.0	0.0
39	0.05815009994548428	0.0	0.0	0.0	0.0
40	0.05815009994548428	0.0	0.0	0.0	0.0
41	0.06178448119207705	0.0	0.0	0.0	0.0
42	0.07268762493185535	0.0	0.0	0.0	0.0
43	0.08359076867163366	0.0	0.0	0.0	0.0
44	0.0908595311648192	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTCCAA	15	0.0076936735	51.033646	38
GTCCAAT	15	0.0076936735	51.033646	39
TCCAATC	15	0.0076936735	51.033646	40
CGATTGA	15	0.0076936735	51.033646	40
ATCCATT	20	3.8200337E-4	50.749077	37
TTGTCCA	15	0.007866122	50.749073	37
TTTTGTC	15	0.007924239	50.65492	35
AAGAGCC	15	0.007924239	50.65492	35
AAGAGAC	15	0.007924239	50.65492	35
AGAGCCC	15	0.007924239	50.65492	36
TTTGTCC	15	0.007924239	50.65492	36
CGACACA	20	3.9627845E-4	50.374542	44
TAAGAGA	20	3.9627845E-4	50.374542	34
GACACAA	15	0.008100508	50.37454	45
TAATCCG	15	0.008100508	50.37454	42
ATTGACC	15	0.008100508	50.37454	42
TATTAAA	15	0.008100508	50.37454	34
AGTTAAA	20	4.0357318E-4	50.189342	49
AGTGGTA	15	0.00834005	50.005497	14
GAGCCAA	15	0.00834005	50.005497	9
>>END_MODULE