FastQCFastQC Report
Fri 17 Jun 2016
SRR1525945_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525945_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3127
Sequences flagged as poor quality0
Sequence length40
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC230.7355292612727854No Hit
GGTACAGGGATGAGATGGACTTAAAGGAATTGACGGGTAC110.35177486408698433No Hit
GGTACAGGGATGAGATGGACTTAAATGAATTGACGGGTAC100.3197953309881676No Hit
GGTACAGGGATGAGATGGCCTTAAAGGAATTGACGGGTAC90.2878157978893508No Hit
GGTACAGGGGGGCTGGTGAGATGGCTCCGTGGGTAAGAGC90.2878157978893508No Hit
GGTACAGGGGGGCTGGTGCGATGGCTCAGTGGGTAAGAGC80.25583626479053406No Hit
GGTACAGGGATGAGATGGACTCAAATGAATTGACGGGTAC70.2238567316917173No Hit
GGTACAGGGATGAGATGGACTCAAAGGAATTGACGGGTAC50.1598976654940838No Hit
GGTACAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT50.1598976654940838No Hit
GGTACAGGGATGAGATGGCCTCAAAGGAATTGACGGGTAC50.1598976654940838No Hit
GGTACAGGGATGAGATGGACTTACAGGAATTGACGGGTAC50.1598976654940838No Hit
GGTACAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT50.1598976654940838No Hit
GGTACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG50.1598976654940838No Hit
GGTACAGGGGGGGCTGGTGAGATGGCTCCGTGGGTAAGAG50.1598976654940838No Hit
GGTACAGGGATGAGATGGCCTCAAATGAATTGACGGGTAC50.1598976654940838No Hit
GGTACAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGA40.12791813239526703No Hit
GGTACAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGC40.12791813239526703No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG559.094947E-1234.4426236
GGATGAG251.4772624E-434.4426238
AGGGATG356.2611434E-734.4426236
GATGAGA251.4772624E-434.4426239
AGGGAAG309.674744E-634.4426236
ATGAGAT251.4772624E-434.44262310
AGGGGAT200.002230882434.442626
ACAGGGA1200.033.88714
GGTACAG3100.033.88711
TACAGGG3050.033.8870963
GACGGGT251.6322122E-433.88709632
AGGAATT200.00242273433.88709625
GTACAGG3050.033.8870962
CGGGTAC251.6322122E-433.88709634
CAGGGGT200.00242273433.8870965
CAGGGGG800.033.8870965
CAGGGGC200.00242273433.8870965
CAGGGGA404.6629793E-833.8870965
CAGGGAT501.891749E-1033.8870965
CAGGGAA452.9922376E-933.8870965
Produced by FastQC (version 0.11.5)