##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1525945_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3127
Sequences flagged as poor quality	0
Sequence length	40
%GC	54
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.72721458266709	30.0	25.0	33.0	4.0	35.0
2	24.29165334186121	27.0	21.0	31.0	4.0	33.0
3	16.892548768787975	15.0	13.0	21.0	4.0	29.0
4	22.96706108090822	26.0	17.0	31.0	4.0	33.0
5	15.2513591301567	15.0	11.0	17.0	4.0	27.0
6	21.68084425967381	24.0	15.0	30.0	4.0	33.0
7	22.078669651423088	25.0	15.0	30.0	4.0	33.0
8	23.757914934441956	28.0	19.0	31.0	4.0	33.0
9	22.856411896386312	27.0	17.0	31.0	4.0	33.0
10	19.278541733290695	20.0	11.0	27.0	4.0	32.0
11	24.801726894787336	29.0	16.0	35.0	4.0	38.0
12	24.03645666773265	28.0	13.0	34.0	4.0	38.0
13	24.675727534377998	29.0	16.0	35.0	4.0	38.0
14	25.21138471378318	30.0	17.0	35.0	4.0	38.0
15	23.216821234409977	27.0	12.0	35.0	4.0	38.0
16	21.876878797569557	26.0	6.0	33.0	4.0	36.0
17	22.541733290693955	28.0	4.0	33.0	4.0	36.0
18	22.344419571474255	27.0	4.0	33.0	4.0	36.0
19	19.34569875279821	21.0	4.0	31.0	4.0	35.0
20	20.795330988167574	25.0	4.0	33.0	4.0	36.0
21	18.162136232811	18.0	4.0	31.0	4.0	35.0
22	17.92484809721778	18.0	4.0	30.0	4.0	35.0
23	17.97217780620403	18.0	4.0	31.0	4.0	35.0
24	17.30892228973457	16.0	4.0	31.0	4.0	35.0
25	16.61976335145507	11.0	4.0	31.0	4.0	35.0
26	13.439078989446754	4.0	4.0	26.0	4.0	33.0
27	13.307962903741606	4.0	4.0	26.0	4.0	33.0
28	12.843300287815797	4.0	4.0	25.0	4.0	33.0
29	12.292932523185161	4.0	4.0	23.0	4.0	33.0
30	11.415733930284619	4.0	4.0	19.0	4.0	32.0
31	10.87240166293572	4.0	4.0	18.0	4.0	31.0
32	10.047329708986249	4.0	4.0	14.0	4.0	30.0
33	9.924848097217781	4.0	4.0	13.0	4.0	30.0
34	9.66837224176527	4.0	4.0	10.0	4.0	30.0
35	9.091781259993605	4.0	4.0	4.0	4.0	29.0
36	8.955868244323632	4.0	4.0	4.0	4.0	30.0
37	8.81323952670291	4.0	4.0	4.0	4.0	30.0
38	8.586824432363288	4.0	4.0	4.0	4.0	29.0
39	8.332267348896707	4.0	4.0	4.0	4.0	28.0
40	8.302526383114806	4.0	4.0	4.0	4.0	30.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	1.0145502645502624
1	2	6.2939902998236334
1	3	7.827839506172836
1	4	9.314268077601408
1	5	2.5216490299823633
1	6	9.844942680776015
1	7	9.98916225749559
1	8	1.9784964726631422
1	9	4.738500881834206
1	10	7.420057319223982
1	11	-8.722288359788363
1	12	8.471851851851856
1	13	-4.493214285714284
1	14	8.167645502645499
1	15	1.669576719576721
1	16	10.509735449735448
1	17	10.175723104056441
1	18	3.1167768959435556
1	19	4.575229276895946
1	20	12.983567019400358
1	21	8.519682539682538
1	22	8.451102292768955
1	23	6.737993827160492
1	24	2.671657848324516
1	25	1.1909347442680769
1	26	-8.60553791887125
1	27	-7.901472663139328
1	28	-8.257385361552032
1	29	-7.295229276895945
1	30	-6.830987654320989
1	31	-5.647328042328041
1	32	-4.934567901234567
1	33	-4.601675485008817
1	34	-4.64099647266314
1	35	-4.116463844797179
1	36	-4.017962962962962
1	37	-3.9582539682539686
1	38	-3.698880070546738
1	39	-3.5457142857142845
1	40	-3.87173721340388
6	1	-2.033068783068785
6	2	-1.4202954144620819
6	3	-5.934065255731925
6	4	-2.590493827160497
6	5	-3.1450176366843046
6	6	3.178276014109347
6	7	-8.43940917107584
6	8	-8.259598765432097
6	9	-4.166261022927699
6	10	3.8010097001763654
6	11	-0.10324074074074474
6	12	1.0908994708994726
6	13	-3.683690476190474
6	14	-6.213306878306884
6	15	2.098148148148148
6	16	-11.775978835978838
6	17	-4.776657848324511
6	18	-4.740365961199302
6	19	-3.900961199294528
6	20	-1.302147266313927
6	21	-1.9088888888888906
6	22	2.1177689594356224
6	23	-2.8334347442680787
6	24	0.14784832451499064
6	25	-1.8566843033509706
6	26	-2.034109347442678
6	27	-0.3300440917107572
6	28	3.5997574955908256
6	29	3.5619135802469124
6	30	1.1690123456790111
6	31	-2.0758994708994702
6	32	-0.36313932980599617
6	33	-2.3159611992945317
6	34	-2.64099647266314
6	35	-0.25932098765432254
6	36	-2.4465343915343905
6	37	-2.3868253968253974
6	38	0.7296913580246907
6	39	0.8828571428571443
6	40	-0.7288800705467366
7	1	5.081216931216929
7	2	3.7939902998236334
7	3	-0.40549382716049465
7	4	7.180934744268075
7	5	3.954982363315697
7	6	3.2782760141093483
7	7	1.089162257495591
7	8	-2.4881701940035263
7	9	0.9051675485008737
7	10	5.386723985890651
7	11	5.011044973544969
7	12	1.6051851851851886
7	13	2.573452380952382
7	14	4.4009788359788296
7	15	6.56957671957672
7	16	5.209735449735447
7	17	7.409056437389772
7	18	3.9167768959435563
7	19	4.9418959435626135
7	20	3.4835670194003576
7	21	4.819682539682539
7	22	4.217768959435624
7	23	2.9379938271604917
7	24	5.60499118165785
7	25	4.7576014109347415
7	26	6.09446208112875
7	27	-1.201472663139329
7	28	-0.3573853615520317
7	29	1.904770723104054
7	30	4.169012345679011
7	31	1.4526719576719582
7	32	3.0654320987654327
7	33	4.998324514991182
7	34	0.9590035273368596
7	35	2.6835361552028214
7	36	2.3820370370370387
7	37	3.7417460317460307
7	38	-1.6988800705467382
7	39	0.6542857142857148
7	40	-1.7717372134038802
9	1	1.6812169312169303
9	2	0.29399029982363345
9	3	-0.0054938271604960676
9	4	2.480934744268076
9	5	-0.4783509700176385
9	6	1.678276014109347
9	7	0.15582892416225747
9	8	0.31182980599647436
9	9	0.9051675485008737
9	10	4.58672398589065
9	11	-2.3889550264550294
9	12	6.13851851851852
9	13	6.840119047619051
9	14	5.834312169312163
9	15	9.169576719576721
9	16	7.676402116402116
9	17	9.175723104056441
9	18	5.950110229276888
9	19	8.741895943562614
9	20	11.983567019400358
9	21	16.686349206349202
9	22	14.617768959435622
9	23	13.737993827160492
9	24	15.671657848324516
9	25	15.190934744268075
9	26	-0.7722045855379154
9	27	-1.9014726631393284
9	28	-2.090718694885366
9	29	-3.961895943562612
9	30	0.002345679012345059
9	31	-3.3139947089947084
9	32	-0.7679012345679013
9	33	-2.6016754850088173
9	34	-3.14099647266314
9	35	-0.28313051146384627
9	36	1.3153703703703723
9	37	1.3750793650793653
9	38	1.6344532627865958
9	39	1.7876190476190494
9	40	1.461596119929454
10	1	-0.6187830687830704
10	2	-0.10600970017636513
10	3	3.994506172839504
10	4	1.3809347442680746
10	5	-2.245017636684304
10	6	-0.7217239858906517
10	7	-0.7108377425044097
10	8	-0.6881701940035256
10	9	-1.1948324514991278
10	10	0.9867239858906487
10	11	3.6110449735449706
10	12	6.7051851851851865
10	13	4.773452380952385
10	14	3.8009788359788317
10	15	2.8695767195767203
10	16	7.1097354497354495
10	17	5.809056437389774
10	18	7.516776895943554
10	19	9.841895943562616
10	20	7.383567019400356
10	21	12.11968253968254
10	22	12.017768959435621
10	23	11.837993827160494
10	24	13.004991181657848
10	25	12.557601410934742
10	26	0.8944620811287507
10	27	1.8985273368606723
10	28	1.3426146384479676
10	29	-1.595229276895946
10	30	-1.5309876543209882
10	31	-2.747328042328041
10	32	0.1654320987654323
10	33	-0.8016754850088175
10	34	-0.24099647266313973
10	35	1.283536155202821
10	36	-2.917962962962962
10	37	-2.858253968253969
10	38	-2.5988800705467385
10	39	-1.1457142857142841
10	40	-2.7717372134038802
12	1	3.6812169312169303
12	2	0.29399029982363345
12	3	10.494506172839504
12	4	-10.019065255731924
12	5	-0.14501763668430456
12	6	-6.821723985890653
12	7	-7.01083774250441
12	8	6.311829805996474
12	9	0.40516754850087366
12	10	0.08672398589065011
12	11	5.611044973544971
12	12	-9.194814814814812
12	13	-11.826547619047616
12	14	-2.499021164021169
12	15	-2.330423280423279
12	16	9.509735449735448
12	17	1.5090564373897735
12	18	10.116776895943556
12	19	10.241895943562614
12	20	3.9835670194003576
12	21	18.519682539682538
12	22	19.117768959435622
12	23	9.737993827160492
12	24	20.00499118165785
12	25	20.85760141093474
12	26	16.39446208112875
12	27	6.098527336860672
12	28	21.742614638447968
12	29	18.704770723104055
12	30	23.16901234567901
12	31	-5.647328042328041
12	32	-4.934567901234567
12	33	-4.601675485008817
12	34	-4.64099647266314
12	35	-4.116463844797179
12	36	-4.017962962962962
12	37	-3.9582539682539686
12	38	-3.698880070546738
12	39	-3.5457142857142845
12	40	-3.87173721340388
13	1	-4.207671957671959
13	2	-2.7060097001763666
13	3	-2.9499382716049407
13	4	-5.463509700176369
13	5	-0.367239858906526
13	6	2.2893871252204576
13	7	-3.0108377425044104
13	8	1.089607583774253
13	9	0.6273897707230951
13	10	0.42005731922398226
13	11	5.277711640211638
13	12	6.360740740740745
13	13	5.395674603174605
13	14	1.9454232804232738
13	15	2.5584656084656103
13	16	7.620846560846559
13	17	4.397945326278663
13	18	9.450110229276888
13	19	8.797451499118171
13	20	8.094678130511468
13	21	9.519682539682538
13	22	8.67332451499118
13	23	8.849104938271603
13	24	14.116102292768959
13	25	12.4131569664903
13	26	8.17223985890653
13	27	8.209638447971782
13	28	4.742614638447968
13	29	10.482548500881833
13	30	6.391234567901233
13	31	5.130449735449737
13	32	4.954320987654322
13	33	2.064991181657849
13	34	3.4701146384479706
13	35	3.77242504409171
13	36	4.6487037037037044
13	37	5.597301587301587
13	38	6.301119929453262
13	39	1.8987301587301602
13	40	2.794929453262786
14	1	-3.3187830687830697
14	2	1.2939902998236334
14	3	1.494506172839504
14	4	2.480934744268076
14	5	3.3549823633156954
14	6	-7.321723985890653
14	7	4.98916225749559
14	8	5.811829805996474
14	9	-2.5948324514991263
14	10	-2.41327601410935
14	11	3.6110449735449706
14	12	6.305185185185188
14	13	-1.3265476190476164
14	14	2.000978835978831
14	15	2.669576719576721
14	16	-0.49026455026455196
14	17	10.509056437389773
14	18	13.116776895943556
14	19	15.741895943562614
14	20	-0.5164329805996424
14	21	3.019682539682538
14	22	1.6177689594356224
14	23	4.237993827160492
14	24	4.504991181657848
14	25	-10.142398589065257
14	26	-8.60553791887125
14	27	-7.901472663139328
14	28	-8.257385361552032
14	29	-7.295229276895945
14	30	-6.830987654320989
14	31	-5.647328042328041
14	32	-4.934567901234567
14	33	-4.601675485008817
14	34	-4.64099647266314
14	35	-4.116463844797179
14	36	-4.017962962962962
14	37	-3.9582539682539686
14	38	-3.698880070546738
14	39	-3.5457142857142845
14	40	-3.87173721340388
15	1	-4.31878306878307
15	2	-4.372676366843034
15	3	-3.172160493827162
15	4	-9.019065255731924
15	5	2.5216490299823633
15	6	-0.15505731922398525
15	7	-0.6775044091710782
15	8	-2.021503527336858
15	9	-0.2614991181657942
15	10	-4.246609347442684
15	11	1.9443783068783027
15	12	2.4718518518518557
15	13	0.8401190476190514
15	14	2.834312169312163
15	15	0.3362433862433889
15	16	5.176402116402116
15	17	4.175723104056441
15	18	3.4501102292768877
15	19	5.241895943562614
15	20	4.983567019400358
15	21	9.186349206349206
15	22	3.4511022927689545
15	23	6.737993827160492
15	24	9.33832451499118
15	25	9.190934744268075
15	26	2.7277954144620846
15	27	2.4318606701940055
15	28	0.7426146384479679
15	29	-2.628562610229279
15	30	2.835679012345677
15	31	1.3526719576719586
15	32	3.0654320987654327
15	33	1.0649911816578488
15	34	5.025670194003526
15	35	-4.116463844797179
15	36	-4.017962962962962
15	37	-3.9582539682539686
15	38	-3.698880070546738
15	39	-3.5457142857142845
15	40	-3.87173721340388
16	1	2.9312169312169303
16	2	4.1689902998236334
16	3	-1.255493827160496
16	4	5.730934744268076
16	5	-0.14501763668430456
16	6	2.803276014109347
16	7	1.9891622574955896
16	8	3.9368298059964744
16	9	2.4051675485008737
16	10	3.96172398589065
16	11	12.98604497354497
16	12	13.305185185185188
16	13	8.298452380952384
16	14	10.750978835978831
16	15	9.919576719576721
16	16	12.759735449735448
16	17	10.009056437389773
16	18	11.741776895943556
16	19	5.991895943562614
16	20	14.108567019400358
16	21	9.769682539682538
16	22	5.367768959435622
16	23	10.362993827160492
16	24	12.004991181657848
16	25	8.357601410934743
16	26	6.894462081128751
16	27	9.098527336860672
16	28	5.867614638447968
16	29	7.954770723104055
16	30	0.9190123456790111
16	31	-5.647328042328041
16	32	-4.934567901234567
16	33	-4.601675485008817
16	34	-4.64099647266314
16	35	-4.116463844797179
16	36	-4.017962962962962
16	37	-3.9582539682539686
16	38	-3.698880070546738
16	39	-3.5457142857142845
16	40	-3.87173721340388
17	1	2.5701058201058196
17	2	0.7384347442680763
17	3	-1.6166049382716068
17	4	4.536490299823633
17	5	-0.8116843033509706
17	6	3.178276014109347
17	7	1.1002733686067003
17	8	1.422940917107585
17	9	-0.0392768959435692
17	10	-0.024387125220460604
17	11	1.9443783068783027
17	12	1.9162962962962986
17	13	-0.048769841269837855
17	14	3.3898677248677203
17	15	3.4473544973544996
17	16	2.7319576719576695
17	17	5.731278659611995
17	18	3.338999118165777
17	19	6.575229276895946
17	20	7.6502336860670255
17	21	2.186349206349206
17	22	8.117768959435622
17	23	6.515771604938271
17	24	-1.883897707231041
17	25	1.4131569664902983
17	26	-5.161093474426805
17	27	0.09852733686067161
17	28	0.18705908289241258
17	29	-2.9618959435626113
17	30	-6.164320987654322
17	31	-2.425105820105819
17	32	-2.045679012345678
17	33	-1.712786596119928
17	34	-2.64099647266314
17	35	-3.1164638447971793
17	36	-3.0179629629629616
17	37	-1.513809523809524
17	38	-1.2544356261022935
17	39	-0.32349206349206217
17	40	-0.42729276895943524
18	1	7.395502645502646
18	2	7.2939902998236334
18	3	1.0659347442680769
18	4	7.409506172839503
18	5	11.283553791887122
18	6	9.463990299823632
18	7	7.703447971781305
18	8	10.45468694885362
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101	7	13.48916225749559
101	8	7.061829805996474
101	9	12.655167548500874
101	10	-0.9132760141093499
101	11	-1.6389550264550294
101	12	-1.194814814814812
101	13	-2.3265476190476164
101	14	3.250978835978831
101	15	3.169576719576721
101	16	1.009735449735448
101	17	4.2590564373897735
101	18	-2.6332231040564444
101	19	3.7418959435626142
101	20	4.483567019400358
101	21	-8.480317460317462
101	22	-9.882231040564378
101	23	-8.012006172839508
101	24	-9.245008818342152
101	25	-5.142398589065257
101	26	-5.605537918871249
101	27	-7.151472663139328
101	28	-5.257385361552032
101	29	-4.295229276895945
101	30	-1.8309876543209889
101	31	-1.8973280423280414
101	32	-0.9345679012345673
101	33	1.6483245149911827
101	34	1.10900352733686
101	35	1.1335361552028207
101	36	3.7320370370370384
101	37	3.7917460317460314
101	38	4.051119929453262
101	39	4.4542857142857155
101	40	3.62826278659612
102	1	6.347883597883598
102	2	10.62732363315697
102	3	3.494506172839504
102	4	12.64760141093474
102	5	13.521649029982363
102	6	10.844942680776015
102	7	9.655828924162257
102	8	9.978496472663139
102	9	11.405167548500874
102	10	6.08672398589065
102	11	-1.0556216931216973
102	12	-3.5281481481481443
102	13	-2.8265476190476164
102	14	-6.165687830687837
102	15	-3.330423280423279
102	16	0.509735449735448
102	17	-0.1576102292768944
102	18	-0.2165564373897766
102	19	-0.7581040564373858
102	20	4.6502336860670255
102	21	7.85301587301587
102	22	7.4511022927689545
102	23	10.071327160493825
102	24	5.004991181657848
102	25	8.52426807760141
102	26	9.727795414462083
102	27	8.431860670194004
102	28	7.742614638447968
102	29	8.704770723104055
102	30	14.502345679012343
102	31	3.6860052910052925
102	32	4.398765432098767
102	33	4.731657848324517
102	34	4.692336860670194
102	35	3.2168694885361546
102	36	-0.35129629629629466
102	37	6.375079365079365
102	38	7.634453262786596
102	39	0.12095238095238248
102	40	6.461596119929454
103	1	0.9312169312169303
103	2	2.7939902998236334
103	3	2.494506172839504
103	4	0.980934744268076
103	5	0.35498236331569544
103	6	1.678276014109347
103	7	4.98916225749559
103	8	-4.188170194003526
103	9	1.6551675485008737
103	10	-3.66327601410935
103	11	0.36104497354497056
103	12	-0.6948148148148121
103	13	-2.8265476190476164
103	14	1.000978835978831
103	15	0.41957671957672105
103	16	4.509735449735448
103	17	4.2590564373897735
103	18	1.6167768959435556
103	19	8.241895943562614
103	20	-2.0164329805996424
103	21	-0.2303174603174618
103	22	0.3677689594356224
103	23	-1.7620061728395076
103	24	-0.4950088183421517
103	25	2.607601410934743
103	26	4.144462081128751
103	27	7.598527336860672
103	28	5.742614638447968
103	29	-1.5452292768959452
103	30	-1.8309876543209889
103	31	-1.3973280423280414
103	32	-2.1845679012345673
103	33	3.1483245149911827
103	34	1.60900352733686
103	35	1.6335361552028207
103	36	0.23203703703703837
103	37	1.5417460317460314
103	38	1.8011199294532618
103	39	-0.5457142857142845
103	40	1.6282627865961201
104	1	5.88121693121693
104	2	4.493990299823633
104	3	6.694506172839503
104	4	5.780934744268077
104	5	3.0549823633156947
104	6	7.978276014109348
104	7	6.589162257495591
104	8	7.511829805996474
104	9	5.205167548500874
104	10	10.686723985890652
104	11	3.011044973544969
104	12	7.005185185185187
104	13	5.773452380952385
104	14	1.9009788359788296
104	15	6.669576719576721
104	16	-1.0902645502645534
104	17	-5.690943562610226
104	18	-2.8832231040564444
104	19	-5.758104056437386
104	20	-7.216432980599642
104	21	-0.08031746031746323
104	22	0.9177689594356231
104	23	1.137993827160491
104	24	-2.7950088183421524
104	25	-0.9423985890652578
104	26	-0.0055379188712496585
104	27	1.8985273368606723
104	28	0.1426146384479683
104	29	-1.095229276895946
104	30	-1.8309876543209889
104	31	0.1526719576719593
104	32	-0.3345679012345677
104	33	-0.6016754850088173
104	34	-2.0409964726631404
104	35	0.08353615520281998
104	36	-1.417962962962962
104	37	-1.5582539682539682
104	38	-3.0988800705467385
104	39	1.6542857142857148
104	40	0.5282627865961205
105	1	-1.6521164021164019
105	2	0.6273236331569656
105	3	-4.505493827160496
105	4	2.314268077601408
105	5	-4.4783509700176385
105	6	2.511609347442679
105	7	-4.677504409171078
105	8	-1.6881701940035256
105	9	-1.2614991181657942
105	10	4.08672398589065
105	11	-8.38895502645503
105	12	-5.86148148148148
105	13	-8.493214285714282
105	14	-9.832354497354503
105	15	-6.997089947089945
105	16	-16.490264550264552
105	17	-17.490943562610227
105	18	-16.883223104056444
105	19	-13.758104056437386
105	20	-15.016432980599642
105	21	-12.480317460317462
105	22	-11.882231040564378
105	23	-11.262006172839508
105	24	-10.995008818342152
105	25	-10.142398589065257
105	26	-8.60553791887125
105	27	-7.901472663139328
105	28	-8.257385361552032
105	29	-7.295229276895945
105	30	-6.830987654320989
105	31	-5.647328042328041
105	32	-4.934567901234567
105	33	-4.601675485008817
105	34	-4.64099647266314
105	35	-4.116463844797179
105	36	-4.017962962962962
105	37	-3.9582539682539686
105	38	-3.698880070546738
105	39	-3.5457142857142845
105	40	-3.87173721340388
106	1	5.18121693121693
106	2	5.7939902998236334
106	3	0.9945061728395039
106	4	0.730934744268076
106	5	8.354982363315695
106	6	2.678276014109347
106	7	-0.010837742504410386
106	8	7.061829805996474
106	9	7.655167548500874
106	10	6.33672398589065
106	11	8.36104497354497
106	12	9.055185185185188
106	13	4.423452380952384
106	14	6.500978835978831
106	15	-3.330423280423279
106	16	9.259735449735448
106	17	8.009056437389773
106	18	5.366776895943556
106	19	-4.258104056437386
106	20	4.983567019400358
106	21	-2.480317460317462
106	22	0.8677689594356224
106	23	-9.262006172839508
106	24	-4.745008818342152
106	25	3.107601410934743
106	26	-5.605537918871249
106	27	-1.1514726631393284
106	28	-8.257385361552032
106	29	-7.295229276895945
106	30	-6.830987654320989
106	31	-5.647328042328041
106	32	-4.934567901234567
106	33	-4.601675485008817
106	34	-4.64099647266314
106	35	-4.116463844797179
106	36	-4.017962962962962
106	37	-3.9582539682539686
106	38	-3.698880070546738
106	39	-3.5457142857142845
106	40	-3.87173721340388
107	1	2.6812169312169303
107	2	4.7939902998236334
107	3	-0.0054938271604960676
107	4	4.980934744268076
107	5	5.354982363315695
107	6	6.678276014109347
107	7	6.48916225749559
107	8	3.8118298059964744
107	9	8.405167548500874
107	10	1.5867239858906501
107	11	6.111044973544971
107	12	10.305185185185188
107	13	4.173452380952384
107	14	-0.49902116402116903
107	15	3.169576719576721
107	16	11.509735449735448
107	17	10.509056437389773
107	18	6.116776895943556
107	19	7.241895943562614
107	20	11.983567019400358
107	21	14.519682539682538
107	22	19.117768959435622
107	23	16.237993827160494
107	24	19.00499118165785
107	25	5.357601410934743
107	26	0.8944620811287507
107	27	-1.4014726631393284
107	28	6.242614638447968
107	29	7.204770723104055
107	30	-6.830987654320989
107	31	-5.647328042328041
107	32	-4.934567901234567
107	33	-4.601675485008817
107	34	-4.64099647266314
107	35	-4.116463844797179
107	36	-4.017962962962962
107	37	-3.9582539682539686
107	38	-3.698880070546738
107	39	-3.5457142857142845
107	40	-3.87173721340388
108	1	9.68121693121693
108	2	12.293990299823633
108	3	16.494506172839504
108	4	4.980934744268076
108	5	17.854982363315695
108	6	-2.821723985890653
108	7	6.98916225749559
108	8	10.311829805996474
108	9	13.405167548500874
108	10	15.08672398589065
108	11	-15.38895502645503
108	12	-5.194814814814812
108	13	10.173452380952384
108	14	1.500978835978831
108	15	-2.330423280423279
108	16	-4.490264550264552
108	17	9.509056437389773
108	18	-16.883223104056444
108	19	-13.758104056437386
108	20	-15.016432980599642
108	21	-12.480317460317462
108	22	-11.882231040564378
108	23	-11.262006172839508
108	24	-10.995008818342152
108	25	-10.142398589065257
108	26	-8.60553791887125
108	27	-7.901472663139328
108	28	-8.257385361552032
108	29	-7.295229276895945
108	30	-6.830987654320989
108	31	-5.647328042328041
108	32	-4.934567901234567
108	33	-4.601675485008817
108	34	-4.64099647266314
108	35	-4.116463844797179
108	36	-4.017962962962962
108	37	-3.9582539682539686
108	38	-3.698880070546738
108	39	-3.5457142857142845
108	40	-3.87173721340388
109	1	3.6812169312169303
109	2	-0.7060097001763666
109	3	-1.505493827160496
109	4	-2.019065255731924
109	5	-2.1450176366843046
109	6	7.178276014109347
109	7	5.98916225749559
109	8	6.311829805996474
109	9	5.405167548500874
109	10	-5.91327601410935
109	11	14.61104497354497
109	12	15.805185185185188
109	13	15.173452380952384
109	14	11.500978835978831
109	15	16.66957671957672
109	16	12.509735449735448
109	17	11.509056437389773
109	18	-16.883223104056444
109	19	-13.758104056437386
109	20	-15.016432980599642
109	21	-12.480317460317462
109	22	-11.882231040564378
109	23	-11.262006172839508
109	24	-10.995008818342152
109	25	-10.142398589065257
109	26	-8.60553791887125
109	27	-7.901472663139328
109	28	-8.257385361552032
109	29	-7.295229276895945
109	30	-6.830987654320989
109	31	-5.647328042328041
109	32	-4.934567901234567
109	33	-4.601675485008817
109	34	-4.64099647266314
109	35	-4.116463844797179
109	36	-4.017962962962962
109	37	-3.9582539682539686
109	38	-3.698880070546738
109	39	-3.5457142857142845
109	40	-3.87173721340388
112	1	-5.31878306878307
112	2	-4.039343033509699
112	3	3.494506172839504
112	4	-4.352398589065256
112	5	-3.8116843033509706
112	6	-6.488390652557319
112	7	-0.010837742504410386
112	8	-8.354836860670192
112	9	-3.9281657848324585
112	10	-7.246609347442684
112	11	-1.0556216931216973
112	12	1.13851851851852
112	13	-0.4932142857142843
112	14	-2.499021164021169
112	15	-2.663756613756611
112	16	3.509735449735448
112	17	2.5090564373897735
112	18	1.4501102292768877
112	19	-3.424770723104052
112	20	-4.6830996472663085
112	21	-6.146984126984128
112	22	-5.882231040564378
112	23	-7.595339506172841
112	24	-5.995008818342152
112	25	-5.142398589065257
112	26	-3.2722045855379154
112	27	-7.234805996472661
112	28	-4.590718694885365
112	29	-4.628562610229278
112	30	-4.830987654320989
112	31	1.0193386243386247
112	32	-1.2679012345679004
112	33	-0.9350088183421503
112	34	2.692336860670194
112	35	2.5502028218694868
112	36	5.6487037037037044
112	37	6.7084126984126975
112	38	5.301119929453262
112	39	5.120952380952382
112	40	5.12826278659612
114	1	0.6812169312169303
114	2	1.2939902998236334
114	3	12.494506172839504
114	4	-4.019065255731924
114	5	-0.14501763668430456
114	6	-12.821723985890653
114	7	5.98916225749559
114	8	6.311829805996474
114	9	0.40516754850087366
114	10	2.08672398589065
114	11	9.61104497354497
114	12	1.805185185185188
114	13	4.173452380952384
114	14	6.500978835978831
114	15	-9.330423280423279
114	16	-7.490264550264552
114	17	0.5090564373897735
114	18	4.116776895943556
114	19	6.241895943562614
114	20	11.983567019400358
114	21	13.519682539682538
114	22	-11.882231040564378
114	23	-11.262006172839508
114	24	-10.995008818342152
114	25	-10.142398589065257
114	26	-8.60553791887125
114	27	-7.901472663139328
114	28	-8.257385361552032
114	29	-7.295229276895945
114	30	-6.830987654320989
114	31	-5.647328042328041
114	32	-4.934567901234567
114	33	-4.601675485008817
114	34	-4.64099647266314
114	35	-4.116463844797179
114	36	-4.017962962962962
114	37	-3.9582539682539686
114	38	-3.698880070546738
114	39	-3.5457142857142845
114	40	-3.87173721340388
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
4	417.0
5	31.0
6	28.0
7	19.0
8	19.0
9	47.0
10	64.0
11	73.0
12	110.0
13	126.0
14	132.0
15	150.0
16	188.0
17	166.0
18	180.0
19	168.0
20	150.0
21	167.0
22	142.0
23	130.0
24	128.0
25	122.0
26	106.0
27	86.0
28	61.0
29	53.0
30	24.0
31	24.0
32	11.0
33	3.0
34	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	100.0	0.0	0.0	0.0
3	0.0	0.0	100.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	0.0	100.0
6	0.0	100.0	0.0	0.0
7	99.03907751441385	0.5765534913516976	0.19218449711723257	0.19218449711723257
8	97.98400000000001	0.608	0.512	0.8959999999999999
9	98.65556978233035	0.44814340588988477	0.3201024327784891	0.5761843790012804
10	48.22513591301567	35.56124080588424	7.131435881036137	9.08218740006396
11	34.98560921010554	23.664854493124402	26.351135273425008	14.998401023345059
12	39.95521433141395	15.131158029430583	22.00895713371721	22.90467050543826
13	26.063319475535657	20.59481931563799	25.295810681164056	28.0460505276623
14	25.775503677646306	21.01055324592261	31.43588103613687	21.77806204029421
15	25.071953949472338	25.90342181004157	28.33386632555165	20.69075791493444
16	30.95618803965462	19.02782219379597	29.9648225135913	20.05116725295811
17	31.307962903741604	21.90598017268948	27.08666453469779	19.69939238887112
18	33.322673488967055	20.243044451551008	25.87144227694276	20.562839782539175
19	23.06456794089303	18.631545133311917	21.554770318021202	36.74911660777385
20	25.295810681164056	24.2404860889031	24.208506555804284	26.255196674128555
21	25.263831148065236	21.714102974096576	30.95618803965462	22.06587783818356
22	21.937959705788295	19.31563799168532	29.517109050207868	29.229293252318516
23	24.400383754397186	24.592260952990085	27.982091461464663	23.025263831148067
24	23.984649824112566	23.664854493124402	26.511032938919094	25.83946274384394
25	24.48	24.512	22.976	28.032
26	21.747510440089947	20.783809829746225	29.521362030195952	27.947317699967876
27	23.128598848368522	18.042226487523994	27.895073576455538	30.93410108765195
28	16.76263595649392	25.463851567498402	26.87140115163148	30.902111324376197
29	22.830611591418506	22.382324687800192	25.77649695805315	29.010566762728146
30	20.14734144778988	16.752081998718772	34.72133247918001	28.379244074311337
31	23.254324151185138	18.225496476617554	34.49711723254324	24.023062139654066
32	29.133354653022064	14.454748960665173	29.10137511992325	27.310521266389507
33	23.600895426926765	18.324272465622002	31.531819635433322	26.543012472017907
34	19.41157659098177	17.300927406459866	35.33738407419252	27.950111928365846
35	21.68212344099776	18.93188359449952	34.921650143907904	24.46434282059482
36	20.81867604732971	22.641509433962266	32.619123760793094	23.920690757914933
37	23.591549295774648	20.198463508322664	32.4583866837388	23.751600512163893
38	19.379597057882954	20.370962583946277	35.04956827630317	25.199872081867603
39	21.618164374800127	21.618164374800127	30.540454109370003	26.223217141029743
40	19.69939238887112	19.475535657179403	29.708986248800766	31.116085705148706
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	4.0
17	8.0
18	8.0
19	9.0
20	10.0
21	10.5
22	11.0
23	11.0
24	18.5
25	26.0
26	28.5
27	31.0
28	31.0
29	35.0
30	39.0
31	42.5
32	46.0
33	46.0
34	48.5
35	51.0
36	75.5
37	100.0
38	100.0
39	104.5
40	109.0
41	132.0
42	155.0
43	155.0
44	169.5
45	184.0
46	199.5
47	215.0
48	215.0
49	234.5
50	254.0
51	236.5
52	219.0
53	219.0
54	220.0
55	221.0
56	224.0
57	227.0
58	227.0
59	221.5
60	216.0
61	245.0
62	274.0
63	274.0
64	264.0
65	254.0
66	238.5
67	223.0
68	223.0
69	169.5
70	116.0
71	95.0
72	74.0
73	74.0
74	58.0
75	42.0
76	28.0
77	14.0
78	14.0
79	9.5
80	5.0
81	3.5
82	2.0
83	2.0
84	1.5
85	1.0
86	0.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.1598976654940838
8	0.06395906619763352
9	0.09593859929645027
10	0.0
11	0.0
12	0.03197953309881676
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.4477134633834346
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.06395906619763352
26	0.4477134633834346
27	0.03197953309881676
28	0.03197953309881676
29	0.12791813239526703
30	0.1598976654940838
31	0.1598976654940838
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.09593859929645027
38	0.0
39	0.0
40	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
40	3127.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	92.99648225135913
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.94222833562586	89.22289734569875
2	3.1292984869325995	5.82027502398465
3	0.34387895460797796	0.9593859929645028
4	0.0687757909215956	0.25583626479053406
5	0.2751031636863824	1.2791813239526704
6	0.0	0.0
7	0.0343878954607978	0.2238567316917173
8	0.0343878954607978	0.25583626479053406
9	0.0687757909215956	0.5756315957787016
>10	0.10316368638239339	1.4070994563479373
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTACAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC	23	0.7355292612727854	No Hit
GGTACAGGGATGAGATGGACTTAAAGGAATTGACGGGTAC	11	0.35177486408698433	No Hit
GGTACAGGGATGAGATGGACTTAAATGAATTGACGGGTAC	10	0.3197953309881676	No Hit
GGTACAGGGATGAGATGGCCTTAAAGGAATTGACGGGTAC	9	0.2878157978893508	No Hit
GGTACAGGGGGGCTGGTGAGATGGCTCCGTGGGTAAGAGC	9	0.2878157978893508	No Hit
GGTACAGGGGGGCTGGTGCGATGGCTCAGTGGGTAAGAGC	8	0.25583626479053406	No Hit
GGTACAGGGATGAGATGGACTCAAATGAATTGACGGGTAC	7	0.2238567316917173	No Hit
GGTACAGGGATGAGATGGACTCAAAGGAATTGACGGGTAC	5	0.1598976654940838	No Hit
GGTACAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT	5	0.1598976654940838	No Hit
GGTACAGGGATGAGATGGCCTCAAAGGAATTGACGGGTAC	5	0.1598976654940838	No Hit
GGTACAGGGATGAGATGGACTTACAGGAATTGACGGGTAC	5	0.1598976654940838	No Hit
GGTACAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT	5	0.1598976654940838	No Hit
GGTACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG	5	0.1598976654940838	No Hit
GGTACAGGGGGGGCTGGTGAGATGGCTCCGTGGGTAAGAG	5	0.1598976654940838	No Hit
GGTACAGGGATGAGATGGCCTCAAATGAATTGACGGGTAC	5	0.1598976654940838	No Hit
GGTACAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGA	4	0.12791813239526703	No Hit
GGTACAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGC	4	0.12791813239526703	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGGGGG	55	9.094947E-12	34.442623	6
GGATGAG	25	1.4772624E-4	34.442623	8
AGGGATG	35	6.2611434E-7	34.442623	6
GATGAGA	25	1.4772624E-4	34.442623	9
AGGGAAG	30	9.674744E-6	34.442623	6
ATGAGAT	25	1.4772624E-4	34.442623	10
AGGGGAT	20	0.0022308824	34.44262	6
ACAGGGA	120	0.0	33.8871	4
GGTACAG	310	0.0	33.8871	1
TACAGGG	305	0.0	33.887096	3
GACGGGT	25	1.6322122E-4	33.887096	32
AGGAATT	20	0.002422734	33.887096	25
GTACAGG	305	0.0	33.887096	2
CGGGTAC	25	1.6322122E-4	33.887096	34
CAGGGGT	20	0.002422734	33.887096	5
CAGGGGG	80	0.0	33.887096	5
CAGGGGC	20	0.002422734	33.887096	5
CAGGGGA	40	4.6629793E-8	33.887096	5
CAGGGAT	50	1.891749E-10	33.887096	5
CAGGGAA	45	2.9922376E-9	33.887096	5
>>END_MODULE