FastQCFastQC Report
Fri 17 Jun 2016
SRR1525943_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525943_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1004
Sequences flagged as poor quality0
Sequence length40
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT201.9920318725099602No Hit
GGTACAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG141.394422310756972No Hit
GGTACAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC121.1952191235059761No Hit
GGTACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG100.9960159362549801No Hit
GGTACAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.5976095617529881No Hit
GGTACAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT50.49800796812749004No Hit
GGTACAGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT50.49800796812749004No Hit
GGTACAGGGACCCACTCCCGACCCGGGGAGGTAGTGACGA40.398406374501992No Hit
GGTACAGGGACTAATCCTAGCCCTAGCCCTACACAAATAT40.398406374501992No Hit
GGTACAGGGGCTTTTTGGGAGTGAGTGTGTTCGTGGTCCC30.29880478087649404No Hit
GGTACAGGGGGAGGTAATATTAAATGATCTCCAGCTATAC30.29880478087649404No Hit
GGTACAGGGGTTATATTTTAGAAAGTTTAACAATGAATTG30.29880478087649404No Hit
GGTACAGGGAGTGAAACTGCGAATGGCTCATTAAATCAGT30.29880478087649404No Hit
GGTACAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT30.29880478087649404No Hit
GGTACAGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG30.29880478087649404No Hit
GGTACAGGGGGCTTAGATCTTTTAGAAGAAAAGAAACCGA30.29880478087649404No Hit
GGTACAGGGAATTGGGTGTTACCATGACCATCCGTATTTG30.29880478087649404No Hit
GGTACAGGGAACCGGAATTGTTTTATCCAACTCATCCCTT30.29880478087649404No Hit
GGTACAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCG20.199203187250996No Hit
GGTACAGGGTTTTGTGGATTCCTCCACTCATGGCTCCTCT20.199203187250996No Hit
GGTACAGGGTCACAGATCCTCTGAAGGAGATTGGGCGGAT20.199203187250996No Hit
GGTACAGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACT20.199203187250996No Hit
GGTACAGGGGTAGTTGGATCTTGGGAGCGGGCGGGCGGTC20.199203187250996No Hit
GGTACAGGGGGGGAATCAGGGTTCGATTCCGGAGAGGGAG20.199203187250996No Hit
GGTACAGGGGAGCTGGAAAGATAGCTCAGCGGTTAAGAGC20.199203187250996No Hit
GGTACAGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAG20.199203187250996No Hit
GGTACAGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGA20.199203187250996No Hit
GGTACAGGGTGGGCCATTGCCCTCTCTGTGGCAGACTTGG20.199203187250996No Hit
GGTACAGGGGTTTCTAAACTGCTGAATCTTGGCCTGATCA20.199203187250996No Hit
GGTACAGGGAGGCTTCACCCTAGATGACACATGAGCAAAA20.199203187250996No Hit
GGTACAGGGATTGAGGTAATAAATTAACTTGTTTTTTAAG20.199203187250996No Hit
GGTACAGGGGGGCTGGAGAGATGGCTCAGCTGTTAAGAGC20.199203187250996No Hit
GGTACAGGGAGGAACTTTTTTTTTGAAGATTTGAAACGAA20.199203187250996No Hit
GGTACAGGGTCACAAATCCTCTTCTGAAGGAGATTGGGCG20.199203187250996No Hit
GGTACAGGGATCTGGGGTTATCTGGGTCTTCAGTACTCTG20.199203187250996No Hit
GGTACAGGGATCCAAGGAAGGCAGCAGGCGCGCAAATTAC20.199203187250996No Hit
GGTACAGGGGGCCGGGGGCATTCGTATTGCGCCGCTAGAG20.199203187250996No Hit
GGTACAGGGTCACAGATCCTCTCCTGAAGGAGATTGGGCG20.199203187250996No Hit
GGTACAGGGGGGAAGGAGGTTCACAAGCAGGTGGTGGACA20.199203187250996No Hit
GGTACAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGA20.199203187250996No Hit
GGTACAGGGGGGATGGTGAGATGGCTCAGTGGGTAAGAGC20.199203187250996No Hit
GGTACAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAG20.199203187250996No Hit
GGTACAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGC20.199203187250996No Hit
GGTACAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGA20.199203187250996No Hit
GGTACAGGGGTCAAAGCCTAGGGGTCCCTGGGTTGGCAGG20.199203187250996No Hit
GGTACAGGGTCACAAATCCTCTTCTGAGATGAGTTTTTGT20.199203187250996No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGT200.001662030434.06
TACAGGG950.034.03
GTACAGG950.034.02
CAGGGGG352.3309076E-734.05
ACAGGGG600.034.04
ACAGGGA259.186756E-534.04
GGTACAG1000.034.01
Produced by FastQC (version 0.11.5)