FastQCFastQC Report
Fri 17 Jun 2016
SRR1525942_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525942_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16040
Sequences flagged as poor quality0
Sequence length56
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCCCGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT960.5985037406483791No Hit
CATCCCGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC650.40523690773067333No Hit
CATCCCGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC610.3802992518703242No Hit
CATCCCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC560.3491271820448878No Hit
CATCCCGGGGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGC500.3117206982543641No Hit
CATCCCGGGGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAA470.29301745635910226No Hit
CATCCCGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATG450.2805486284289277No Hit
CATCCCGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCC390.243142144638404No Hit
CATCCCGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC330.2057356608478803No Hit
CATCCCGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT330.2057356608478803No Hit
CATCCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC330.2057356608478803No Hit
CATCCCGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA320.199501246882793No Hit
CATCCCGGGGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTAC320.199501246882793No Hit
CATCCCGGGGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAG300.18703241895261846No Hit
CATCCCGGGGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGT300.18703241895261846No Hit
CATCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC290.18079800498753118No Hit
CATCCCGGGATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGG270.16832917705735662No Hit
CATCCCGGGGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCAT260.1620947630922693No Hit
CATCCCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGACCTAATACATGCCGAC240.14962593516209477No Hit
CATCCCGGGCTCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCG220.1371571072319202No Hit
CATCCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG220.1371571072319202No Hit
CATCCCGGGACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTT220.1371571072319202No Hit
CATCCCGGGGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTT220.1371571072319202No Hit
CATCCCGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACCACCCATTCGAACGTC220.1371571072319202No Hit
CATCCCGGGAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTG210.13092269326683292No Hit
CATCCCGGGGGGGAGGTAGTGACGAAAAATAACAATACAGCACTCTTTCGAGGCCC210.13092269326683292No Hit
CATCCCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGCGCTAATACATGCCGAC200.12468827930174563No Hit
CATCCCGGGGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGA200.12468827930174563No Hit
CATCCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC190.11845386533665835No Hit
CATCCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT190.11845386533665835No Hit
CATCCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGT180.11221945137157108No Hit
CATCCCGGGGACGCTACTCAGACTACCCAGATGCTTACACCACATGAAACACTGTC180.11221945137157108No Hit
CATCCCGGGGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATG180.11221945137157108No Hit
CATCCCGGGAAGGAGGGTTAGGATTGCTCCTTTTCCTTTGCTTCCAGTTATGGTGA170.1059850374064838No Hit
CATCCCGGGATCAGATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGG170.1059850374064838No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATCC203.743128E-450.8242841
AATCCGC150.007858274550.6624243
TTCAATC150.007858274550.6624240
CAATCCG150.007858274550.6624242
TCCGCCC150.007858274550.6624245
ATCCGCC150.007858274550.6624244
CCGCCCA150.00795814750.50158746
GTTCAAT150.00795814750.50158739
CGCCCAA150.00816072250.1829647
ACAGATC204.0496237E-450.02515812
GCCCAAT150.00826343650.02515848
TTATCAG204.0496237E-450.02515848
GTTTTTG251.9892734E-550.02515833
GAGTTCC150.00826343650.02515810
CCCAATC150.00826343650.02515849
GATCCTC251.9892734E-550.02515815
TATCAGA204.0496237E-450.02515849
CAGATCC204.0496237E-450.02515813
GCTCCTA354.841786E-850.0251589
AGATCCT150.00826343650.02515814
Produced by FastQC (version 0.11.5)