##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1525942_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 16040 Sequences flagged as poor quality 0 Sequence length 56 %GC 57 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.668952618453865 39.0 38.0 40.0 34.0 40.0 2 35.714837905236905 39.0 36.0 39.0 26.0 40.0 3 37.05174563591022 39.0 38.0 40.0 33.0 40.0 4 36.73042394014963 39.0 38.0 40.0 31.0 40.0 5 37.042394014962596 39.0 38.0 40.0 33.0 40.0 6 36.749376558603494 39.0 38.0 40.0 31.0 40.0 7 36.601059850374064 39.0 36.0 39.0 32.0 40.0 8 36.73291770573566 39.0 36.0 39.0 32.0 40.0 9 36.76390274314215 39.0 37.0 39.0 32.0 40.0 10 35.841022443890274 39.0 35.0 39.0 29.0 40.0 11 35.530299251870325 38.0 35.0 39.0 29.0 40.0 12 34.000623441396506 38.0 33.0 39.0 23.0 40.0 13 33.57886533665835 38.0 32.0 39.0 21.0 40.0 14 34.14968827930175 38.0 33.0 39.0 23.0 40.0 15 33.325 38.0 32.0 39.0 18.0 40.0 16 32.31172069825436 36.0 29.0 39.0 18.0 40.0 17 31.42699501246883 36.0 27.0 39.0 15.0 40.0 18 30.68148379052369 35.0 26.0 38.0 13.0 40.0 19 31.974625935162095 36.0 29.0 39.0 17.0 40.0 20 32.437655860349125 36.0 29.0 39.0 20.0 40.0 21 32.46508728179551 37.0 29.0 39.0 18.0 40.0 22 31.6963216957606 36.0 28.0 39.0 15.0 40.0 23 32.39925187032419 36.0 30.0 39.0 18.0 40.0 24 31.743890274314214 36.0 28.0 39.0 15.0 40.0 25 32.18840399002494 36.0 29.0 39.0 18.0 40.0 26 32.79675810473815 37.0 31.0 39.0 18.0 40.0 27 32.88784289276808 37.0 31.0 39.0 19.0 40.0 28 32.915274314214464 37.0 31.0 39.0 19.0 40.0 29 32.45062344139651 36.0 31.0 39.0 17.0 40.0 30 32.1038029925187 36.0 30.0 39.0 16.0 40.0 31 33.099501246882795 37.0 32.0 39.0 19.0 40.0 32 32.93036159600997 37.0 32.0 39.0 18.0 40.0 33 32.755299251870326 37.0 32.0 39.0 18.0 40.0 34 32.623254364089775 36.0 32.0 39.0 17.0 40.0 35 32.37899002493766 36.0 31.0 39.0 15.0 40.0 36 31.62356608478803 35.0 30.0 38.0 11.0 40.0 37 31.46577306733167 35.0 30.0 38.0 9.0 40.0 38 30.80997506234414 35.0 29.0 38.0 4.0 40.0 39 30.06490024937656 35.0 28.0 38.0 4.0 39.0 40 27.64993765586035 33.0 19.0 38.0 4.0 39.0 41 25.994887780548627 31.0 16.0 37.0 4.0 39.0 42 26.95162094763092 32.0 20.0 37.0 4.0 39.0 43 26.639401496259353 32.0 19.0 37.0 4.0 39.0 44 25.754551122194513 31.0 16.0 36.0 4.0 39.0 45 26.66209476309227 32.0 20.0 36.0 4.0 39.0 46 24.746072319201996 29.0 15.0 35.0 4.0 39.0 47 23.66215710723192 28.0 9.0 35.0 4.0 39.0 48 24.30785536159601 29.0 11.0 35.0 4.0 39.0 49 23.166832917705737 27.0 6.0 35.0 4.0 38.0 50 23.883728179551124 29.0 4.0 35.0 4.0 38.0 51 24.030236907730675 30.0 4.0 35.0 4.0 38.0 52 23.604613466334165 29.0 4.0 35.0 4.0 38.0 53 23.384725685785536 29.0 4.0 35.0 4.0 38.0 54 23.091708229426434 28.0 4.0 35.0 4.0 38.0 55 22.746758104738156 28.0 4.0 35.0 4.0 38.0 56 22.534912718204488 28.0 4.0 35.0 4.0 38.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 2.0407525881190125 1 2 4.661910930844336 1 3 2.805689408987 1 4 3.033060835370833 1 5 2.8615990385107324 1 6 3.2771303751896284 1 7 2.8223109192384044 1 8 2.416085119244194 1 9 2.8984481353168547 1 10 3.091857844046608 1 11 0.12570522674241147 1 12 -6.760403496012557 1 13 -20.785840403886493 1 14 -7.568508921543234 1 15 1.2362314529612277 1 16 -0.7802058332043664 1 17 -5.3468723676767596 1 18 -4.656745280900488 1 19 0.9330045646495329 1 20 -1.035902162091439 1 21 6.824961622672657 1 22 8.490549945656547 1 23 7.610090008687351 1 24 8.591178721656036 1 25 7.885067884874921 1 26 7.911954295306373 1 27 6.013662477050595 1 28 6.901239426801766 1 29 7.693504998183801 1 30 7.39690018953112 1 31 5.383466286005753 1 32 5.72424970190696 1 33 5.9365519956328185 1 34 6.4941181121942755 1 35 4.731889122602492 1 36 5.175187768055725 1 37 4.585152722313012 1 38 4.648207533384543 1 39 5.46231968200183 1 40 -9.878633902230774 1 41 2.303474083794878 1 42 2.009949464839849 1 43 -7.681996592016876 1 44 -13.001209298284701 1 45 -0.05762215864717746 1 46 -2.627538250710291 1 47 -8.700888384409469 1 48 -3.1634506957156177 1 49 -12.539307449555139 1 50 -2.186968367938295 1 51 4.7328047030309826 1 52 6.439173578121508 1 53 5.186924619831743 1 54 5.907762870033704 1 55 6.6336821361784395 1 56 10.018793092539592 2 1 2.582974810341234 2 2 4.802651671585075 2 3 3.42791163120922 2 4 3.627134909444912 2 5 3.366784223695916 2 6 3.745278523337774 2 7 3.2460146229421056 2 8 3.1197888229478963 2 9 3.455485172353896 2 10 4.204450436639206 2 11 2.666445967483149 2 12 -1.2892923849014473 2 13 -0.09695151499760257 2 14 3.6596392266049094 2 15 4.176972193701971 2 16 3.0538682408697113 2 17 6.522757261952865 2 18 4.1936250894698865 2 19 4.364115675760647 2 20 4.6189126527233775 2 21 7.275331993043029 2 22 8.152772167878766 2 23 7.485645564242908 2 24 8.192660203137514 2 25 6.3102530700601065 2 26 6.737139480491557 2 27 6.738106921495039 2 28 6.501239426801767 2 29 6.742393887072684 2 30 7.045789078420004 2 31 5.490132952672415 2 32 6.124249701906958 2 33 5.475811254892079 2 34 4.920784778860938 2 35 5.303740974454346 2 36 7.090743323611282 2 37 5.862189759350052 2 38 6.553392718569725 2 39 7.857875237557391 2 40 5.860625357028486 2 41 9.870881491202283 2 42 8.81291242780281 2 43 7.43355896353868 2 44 -2.327135224210629 2 45 7.800155619130599 2 46 8.809498786326746 2 47 3.7524449489238627 2 48 8.529882637617714 2 49 3.1747666245189343 2 50 7.395253854283926 2 51 10.91058248080876 2 52 9.92213654108447 2 53 10.43433202723915 2 54 10.41294805521889 2 55 10.958126580622881 2 56 11.152126425872925 3 1 2.527419254785677 3 2 4.4785775975109985 3 3 3.164022742320334 3 4 3.6363941687041716 3 5 3.1399323718440684 3 6 3.7221303751896286 3 7 3.0423109192384032 3 8 3.041085119244194 3 9 3.085114801983522 3 10 3.903524510713275 3 11 4.134038560075744 3 12 4.849596503987442 3 13 4.389159596113508 3 14 4.5948244117901 3 15 -0.7304352137054373 3 16 6.896460833462299 3 17 7.893127632323239 3 18 8.406588052432848 3 19 6.836337897982869 3 20 6.0124311712418965 3 21 7.946628289339319 3 22 7.13888327898988 3 23 7.805090008687351 3 24 7.419512054989369 3 25 6.625067884874923 3 26 6.510287628639709 3 27 6.5019958103839315 3 28 6.334572760135103 3 29 5.686838331517134 3 30 6.656900189531118 3 31 5.781799619339086 3 32 5.6659163685736225 3 33 5.758218662299484 3 34 3.837451445527609 3 35 3.910222455935827 3 36 6.99352110138906 3 37 6.491819388979678 3 38 7.034874200051206 3 39 7.1773196820018335 3 40 9.934699431102562 3 41 11.213474083794878 3 42 12.308282798173185 3 43 10.044670074649787 3 44 11.473790701715298 3 45 10.939044508019485 3 46 11.855795082623043 3 47 11.460778282257195 3 48 11.779882637617717 3 49 10.76735921711153 3 50 10.548031632061704 3 51 11.716138036364317 3 52 11.459173578121508 3 53 9.526924619831743 3 54 10.477762870033704 3 55 9.430348802845106 3 56 10.527126425872925 4 1 2.2807525881190145 4 2 4.3819109308443345 4 3 2.805689408987 4 4 3.3930608353708394 4 5 2.5815990385107312 4 6 3.0771303751896255 4 7 1.5823109192384024 4 8 1.2560851192441973 4 9 2.5784481353168545 4 10 2.691857844046609 4 11 3.805705226742411 4 12 5.239596503987443 4 13 6.454159596113506 4 14 4.471491078456765 4 15 5.876231452961228 4 16 7.699794166795634 4 17 9.13312763232324 4 18 9.26325471909951 4 19 7.333004564649531 4 20 7.364097837908563 4 21 7.7849616226726575 4 22 8.170549945656546 4 23 6.730090008687348 4 24 7.791178721656031 4 25 6.845067884874922 4 26 7.191954295306374 4 27 6.253662477050597 4 28 6.861239426801767 4 29 7.213504998183797 4 30 7.75690018953112 4 31 5.543466286005749 4 32 5.964249701906962 4 33 4.496551995632821 4 34 6.614118112194273 4 35 5.5318891226024896 4 36 7.175187768055725 4 37 6.745152722313016 4 38 7.008207533384542 4 39 7.5423196820018354 4 40 -0.4786339022307722 4 41 10.42347408379488 4 42 10.48994946483985 4 43 8.95800340798312 4 44 10.278790701715298 4 45 9.862377841352824 4 46 11.29246174928971 4 47 11.219111615590528 4 48 11.91654930428438 4 49 12.980692550444864 4 50 12.413031632061706 4 51 12.292804703030978 4 52 12.87917357812151 4 53 12.666924619831743 4 54 11.587762870033703 4 55 13.193682136178438 4 56 12.538793092539592 5 1 2.860752588119013 5 2 5.14524426417767 5 3 3.497356075653663 5 4 4.053060835370836 5 5 3.6815990385107327 5 6 4.055463708522964 5 7 3.0839775859050675 5 8 3.707751785910858 5 9 3.793448135316858 5 10 4.945191177379947 5 11 4.800705226742409 5 12 6.099596503987442 5 13 6.139159596113508 5 14 5.9698244117901 5 15 6.352898119627895 5 16 8.5214608334623 5 17 9.643127632323239 5 18 10.156588052432848 5 19 9.003004564649533 5 20 8.387431171241897 5 21 8.86329495600599 5 22 9.76388327898988 5 23 8.430090008687351 5 24 8.919512054989369 5 25 9.041734551541587 5 26 7.926954295306373 5 27 8.168662477050596 5 28 7.5429060934684315 5 29 7.145171664850462 5 30 8.448566856197782 5 31 6.240132952672415 5 32 7.5409163685736225 5 33 7.133218662299484 5 34 7.545784778860938 5 35 7.285222455935827 5 36 8.576854434722389 5 37 8.700152722313014 5 38 9.243207533384542 5 39 10.38565301533517 5 40 11.76803276443589 5 41 14.630140750461543 5 42 13.474949464839849 5 43 13.961336741316458 5 44 14.640457368381963 5 45 14.564044508019485 5 46 15.522461749289707 5 47 16.335778282257195 5 48 15.529882637617717 5 49 16.559025883778197 5 50 15.923031632061704 5 51 15.674471369697645 5 52 15.750840244788172 5 53 15.110257953165078 5 54 16.81109620336704 5 55 15.805348802845103 5 56 15.693793092539593 6 1 2.235752588119013 6 2 4.7285775975109985 6 3 3.3306894089869985 6 4 3.5947275020375002 6 5 2.8899323718440684 6 6 3.8054637085229643 6 7 2.0423109192384032 6 8 3.207751785910858 6 9 2.8767814686501936 6 10 3.1951911773799466 6 11 2.967371893409073 6 12 5.057929837320778 6 13 5.847492929446844 6 14 3.386491078456764 6 15 6.436231452961231 6 16 7.979794166795635 6 17 8.601460965656575 6 18 9.281588052432848 6 19 8.336337897982869 6 20 7.554097837908561 6 21 7.529961622672655 6 22 8.76388327898988 6 23 6.388423342020687 6 24 8.252845388322697 6 25 5.625067884874923 6 26 7.135287628639709 6 27 6.83532914371726 6 28 6.334572760135103 6 29 6.978504998183798 6 30 6.5735668561977825 6 31 5.6984662860057504 6 32 4.4159163685736225 6 33 2.8415519956328197 6 34 5.045784778860938 6 35 5.785222455935827 6 36 5.74352110138906 6 37 5.741819388979678 6 38 4.493207533384542 6 39 7.1773196820018335 6 40 3.3096994311025583 6 41 11.546807417128214 6 42 11.266616131506513 6 43 10.586336741316458 6 44 10.848790701715298 6 45 9.355711174686157 6 46 12.272461749289707 6 47 12.877444948923866 6 48 10.11321597095105 6 49 12.225692550444862 6 50 12.38136496539504 6 51 12.466138036364317 6 52 10.04250691145484 6 53 11.360257953165078 6 54 11.56109620336704 6 55 9.430348802845106 6 56 6.777126425872925 7 1 2.31727432724945 7 2 4.974954409105202 7 3 3.336124191595694 7 4 3.6400173571099685 7 5 2.9642077341629047 7 6 3.8579999404070193 7 7 2.4988326583688405 7 8 3.024780771418108 7 9 3.6793177005342486 7 10 4.437944800568353 7 11 4.230053052829362 7 12 4.599596503987442 7 13 6.014159596113508 7 14 4.8158389045437175 7 15 6.914492322526449 7 16 7.84935938418694 7 17 9.15399719754063 7 18 9.77977645822995 7 19 7.861700216823447 7 20 6.891054359647693 7 21 7.948439883542218 7 22 8.017506467395677 7 23 7.799655226078656 7 24 7.711178721656033 7 25 7.212024406614056 7 26 7.20412820834985 7 27 6.684966824876682 7 28 6.153413339845244 7 29 7.201331085140321 7 30 6.156900189531118 7 31 4.519118459918797 7 32 5.689467093211309 7 33 4.173073734763257 7 34 4.428031155672532 7 35 2.147541296515538 7 36 5.056926898490506 7 37 5.738196200573881 7 38 5.063859707297588 7 39 5.563189247219224 7 40 5.036148706464878 7 41 9.680865388142706 7 42 9.023862508318107 7 43 8.443220799287474 7 44 8.337921136497908 7 45 8.718030015265867 7 46 10.424635662333184 7 47 10.89737248515575 7 48 10.587853652110468 7 49 11.57895342001008 7 50 10.042596849453009 7 51 10.263239485639676 7 52 8.959173578121508 7 53 11.24431592417957 7 54 10.923415043946747 7 55 12.165856049221915 7 56 10.036184396887418 8 1 2.527419254785677 8 2 3.6035775975109985 8 3 3.247356075653663 8 4 3.6363941687041716 8 5 3.1399323718440684 8 6 3.1804637085229643 8 7 0.8339775859050675 8 8 0.7077517859108582 8 9 1.7934481353168579 8 10 2.1951911773799466 8 11 3.5090385600757443 8 12 4.641263170654106 8 13 5.472492929446844 8 14 3.5948244117901 8 15 5.394564786294566 8 16 6.188127500128971 8 17 6.768127632323239 8 18 8.406588052432848 8 19 4.628004564649533 8 20 6.804097837908561 8 21 6.363294956005991 8 22 6.055549945656544 8 23 5.013423342020687 8 24 6.836178721656033 8 25 6.250067884874923 8 26 4.968620961973038 8 27 5.08532914371726 8 28 5.0429060934684315 8 29 5.728504998183798 8 30 5.240233522864454 8 31 4.3234662860057504 8 32 3.4159163685736225 8 33 2.9248853289661554 8 34 4.6291181121942735 8 35 2.410222455935827 8 36 4.910187768055724 8 37 4.90848605564635 8 38 4.868207533384542 8 39 5.843986348668498 8 40 -0.48196723556410603 8 41 9.463474083794878 8 42 6.391616131506517 8 43 6.836336741316455 8 44 6.640457368381966 8 45 6.855711174686153 8 46 8.939128415956375 8 47 8.627444948923863 8 48 8.446549304284382 8 49 10.725692550444862 8 50 9.964698298728372 8 51 7.716138036364313 8 52 7.91750691145484 8 53 7.2769246198317425 8 54 7.561096203367036 8 55 7.0136821361784385 8 56 6.068793092539593 9 1 1.9694482402929268 9 2 5.018432669974771 9 3 3.4230807133348264 9 4 3.726973878849094 9 5 3.3989903428585606 9 6 3.8145216795374566 9 7 3.2814413540210126 9 8 3.372606858374631 9 9 3.4619263961864206 9 10 4.394466539698783 9 11 4.838748705003276 9 12 5.164813895291793 9 13 6.275029161330899 9 14 5.8158389045437175 9 15 6.001448844265582 9 16 8.675446340708682 9 17 8.545301545366716 9 18 9.388472110403864 9 19 8.122569782040838 9 20 7.586706533560733 9 21 8.513657274846569 9 22 9.62620211956959 9 23 7.799655226078656 9 24 8.66770046078647 9 25 8.429415710961877 9 26 6.812823860523764 9 27 7.119749433572338 9 28 7.675152470280025 9 29 8.027418041662056 9 30 6.548204537357204 9 31 3.7799880251361877 9 32 4.689467093211309 9 33 4.086117213024124 9 34 3.993248546976883 9 35 4.147541296515538 9 36 5.622144289794857 9 37 5.781674461443451 9 38 5.716033620341065 9 39 6.99797185591488 9 40 3.6448443586387924 9 41 7.767821909881835 9 42 8.632558160492021 9 43 8.79104688624399 9 44 9.207486353889212 9 45 8.891943058744125 9 46 11.250722618854926 9 47 12.071285528634007 9 48 11.63133191298003 9 49 10.274605593923123 9 50 11.738249023366055 9 51 10.6110655725962 9 52 11.524390969425859 9 53 11.070402880701305 9 54 9.87993678307718 9 55 10.209334310091482 9 56 7.9492278751482885 10 1 1.1868395446407547 10 2 4.235823974322592 10 3 1.8578633220304752 10 4 2.6834956179795313 10 5 3.0511642559020373 10 6 3.0319129838852774 10 7 2.3249196148905753 10 8 2.8943459888094125 10 9 2.9401872657516392 10 10 3.9162056701335644 10 11 3.5778791397858853 10 12 4.556118243117879 10 13 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114 51 8.775661845888123 114 52 9.744887863835793 114 53 6.8840674769746 114 54 6.942048584319419 114 55 7.370824993321296 114 56 11.24736452111102 115 1 2.235752588119013 115 2 4.249410930844334 115 3 3.0806894089869985 115 4 3.428060835370836 115 5 2.9315990385107327 115 6 3.1596303751896286 115 7 2.8548109192384032 115 8 3.103585119244194 115 9 3.293448135316858 115 10 4.299357844046611 115 11 2.3007052267424086 115 12 -2.150403496012558 115 13 -1.7358404038864919 115 14 -6.863508921543236 115 15 -8.12626854703877 115 16 0.8547941667956351 115 17 3.393127632323239 115 18 4.073254719099513 115 19 1.5030045646495331 115 20 2.866597837908561 115 21 6.529961622672655 115 22 6.868049945656544 115 23 5.992590008687351 115 24 6.148678721656033 115 25 5.625067884874923 115 26 5.614454295306373 115 27 3.481162477050596 115 28 5.688739426801767 115 29 3.728504998183798 115 30 6.031900189531118 115 31 5.2609662860057504 115 32 6.061749701906958 115 33 5.52905199563282 115 34 5.6916181121942735 115 35 6.264389122602491 115 36 6.160187768055724 115 37 7.262652722313014 115 38 5.493207533384542 115 39 6.4898196820018335 115 40 9.788866097769226 115 41 8.275974083794878 115 42 5.037449464839849 115 43 7.9405034079831225 115 44 0.47379070171529847 115 45 6.959877841352821 115 46 -0.16503825071029254 115 47 -9.39338838440947 115 48 -0.3659506957156182 115 49 -4.149307449555138 115 50 1.7980316320617042 115 51 3.382804703030981 115 52 2.834173578121508 115 53 0.15192461983174255 115 54 -1.647237129966296 115 55 -0.7363178638215615 115 56 1.5062930925395932 116 1 2.360752588119013 116 2 5.111910930844331 116 3 3.2556894089869957 116 4 3.503060835370839 116 5 2.98159903851073 116 6 3.647130375189626 116 7 2.6423109192384047 116 8 3.0160851192441953 116 9 2.718448135316855 116 10 2.8118578440466138 116 11 2.475705226742406 116 12 2.599596503987442 116 13 4.014159596113508 116 14 4.411491078456763 116 15 -1.6637685470387709 116 16 5.529794166795632 116 17 7.343127632323242 116 18 6.673254719099514 116 19 6.353004564649535 116 20 6.454097837908559 116 21 7.154961622672655 116 22 8.230549945656541 116 23 7.030090008687353 116 24 6.76117872165603 116 25 5.775067884874922 116 26 4.301954295306373 116 27 5.143662477050597 116 28 5.251239426801767 116 29 4.853504998183798 116 30 6.00690018953112 116 31 1.8734662860057476 116 32 3.374249701906958 116 33 3.016551995632817 116 34 1.0041181121942735 116 35 3.7518891226024884 116 36 4.935187768055723 116 37 6.175152722313015 116 38 5.26820753338454 116 39 5.102319682001831 116 40 6.251366097769225 116 41 6.513474083794879 116 42 3.049949464839848 116 43 1.8280034079831218 116 44 -0.7012092982847022 116 45 2.522377841352821 116 46 0.922461749289706 116 47 -9.030888384409469 116 48 -0.9534506957156168 116 49 -3.049307449555137 116 50 -1.276968367938295 116 51 -0.6171952969690189 116 52 0.7591735781215085 116 53 0.37692461983174397 116 54 1.477762870033704 116 55 -0.4863178638215615 116 56 4.618793092539594 117 1 1.2838295111959326 117 2 3.908064776998181 117 3 2.1287663320639183 117 4 2.3992146815246826 117 5 2.1046759615876525 117 6 2.366361144420395 117 7 2.38846476539225 117 8 2.1083928115518873 117 9 2.3415250583937777 117 10 2.9964732286619977 117 11 -0.5358332347960513 117 12 -10.400403496012558 117 13 -6.678148096194185 117 14 -2.7962012292355425 117 15 -7.1022300855003095 117 16 -8.404821217819748 117 17 1.7392814784770856 117 18 -3.0998222039774106 117 19 2.1760814877264565 117 20 -0.3497483159375925 117 21 4.828038545749575 117 22 5.776703791810391 117 23 5.545474624071964 117 24 6.788101798579113 117 25 4.97122173102877 117 26 6.475031218383293 117 27 6.293662477050596 117 28 5.501239426801767 117 29 5.238120382799185 117 30 4.541515574146505 117 31 5.131158593698057 117 32 4.431942009599265 117 33 4.447321226402053 117 34 5.10027195834812 117 35 5.5288121995255715 117 36 6.919803152671111 117 37 4.978998876159167 117 38 6.829745994923002 117 39 6.763858143540293 117 40 7.678289174692306 117 41 3.6173202376410316 117 42 4.388411003301389 117 43 -3.045073515093801 117 44 -1.7089016059770081 117 45 3.3300701490451274 117 46 -2.535230558402599 117 47 -0.9655037690248527 117 48 -3.3419122341771583 117 49 -4.130076680324368 117 50 -0.28850682947675566 117 51 5.286650856877134 117 52 1.8053274242753545 117 53 1.2576938506009725 117 54 1.3816090238798573 117 55 2.2829129054092085 117 56 4.357254631001133 118 1 0.2698434972099193 118 2 1.9710018399352407 118 3 1.0238712271688186 118 4 1.371242653552656 118 5 0.7270535839652794 118 6 0.5971303751896286 118 7 1.7241291010565831 118 8 1.3251760283351004 118 9 1.4184481353168579 118 10 2.1573123895011577 118 11 -1.3470220459848647 118 12 -13.127676223285285 118 13 -10.531294949341039 118 14 -8.124872557906873 118 15 -12.745586728856953 118 16 -5.202024015022548 118 17 -0.19778145858585106 118 18 -8.35856346271867 118 19 -1.7469954353504669 118 20 0.34955238336310757 118 21 4.268597986309022 118 22 6.566913582020177 118 23 2.111908190505531 118 24 5.711178721656033 118 25 4.306886066693103 118 26 5.46104520439728 118 27 5.475480658868776 118 28 5.410330335892674 118 29 2.7625959072747044 118 30 6.4296274622583915 118 31 4.187102649642117 118 32 4.033340610997865 118 33 5.03473381381464 118 34 5.890481748557907 118 35 1.6337073044206711 118 36 4.3533695862375446 118 37 4.461516358676647 118 38 4.368207533384542 118 39 4.938683318365467 118 40 8.23772973413286 118 41 4.099837720158515 118 42 5.304494919385302 118 43 2.696185226164939 118 44 4.5078816108062085 118 45 4.386014204989184 118 46 -4.409356432528476 118 47 -2.853615657136743 118 48 -3.8034506957156182 118 49 -7.262943813191502 118 50 -4.099695640665569 118 51 1.4055319757582545 118 52 1.7773553963033244 118 53 1.9360155289226526 118 54 2.7732174154882507 118 55 2.695500317996622 118 56 6.1824294561759565 119 1 0.7178954452618669 119 2 3.20476807370148 119 3 1.5628322661298526 119 4 1.91020369251369 119 5 1.5387418956535868 119 6 1.9542732323324827 119 7 1.6851680620955491 119 8 1.273227976387048 119 9 1.775590992459712 119 10 2.611857844046611 119 11 -0.9314376304004455 119 12 -16.82897492458399 119 13 -5.057268975315065 119 14 -7.8456517786860935 119 15 -9.706625689895912 119 16 -8.377348690347223 119 17 -2.035443796248188 119 18 -10.24817385232906 119 19 -1.8898525782076092 119 20 -2.9101878763771545 119 21 4.476390194101224 119 22 5.716264231370829 119 23 4.358661437258782 119 24 3.6397501502274636 119 25 4.982210742017777 119 26 5.266240009592089 119 27 3.793662477050596 119 28 4.644096569658913 119 29 6.424933569612367 119 30 4.299757332388264 119 31 3.5377520002914657 119 32 2.409963987621243 119 33 4.787980567061389 119 34 3.468403826479989 119 35 3.4518891226024913 119 36 5.606616339484294 119 37 5.682295579455868 119 38 5.082493247670257 119 39 7.230891110573264 119 40 8.672794669197796 119 41 5.034902655223451 119 42 6.2070923219827066 119 43 3.092289122268838 119 44 -1.4012092982847015 119 45 4.165234984209963 119 46 0.1296046064325651 119 47 -0.2237455272666118 119 48 -5.803450695715618 119 49 -1.1135931638408536 119 50 -0.32696836793829576 119 51 4.132804703030981 119 52 5.887745006692935 119 53 5.241210334117458 119 54 3.656334298605131 119 55 3.727967850464154 119 56 8.74736452111102 120 1 -1.3059140785476515 120 2 1.7285775975109985 120 3 0.0390227423203342 120 4 1.2197275020375002 120 5 0.848265705177397 120 6 1.263797041856293 120 7 0.12564425257173895 120 8 -0.1672482140891418 120 9 0.001781468650193574 120 10 2.111857844046611 120 11 -5.574294773257591 120 12 -15.067070162679226 120 13 -17.402507070553156 120 14 -14.571842254876568 120 15 -5.313768547038769 120 16 -9.686872499871033 120 17 -2.356872367676761 120 18 -9.51007861423382 120 19 -3.9136621020171347 120 20 -3.695902162091439 120 21 1.9882949560059906 120 22 4.8472166123232086 120 23 4.013423342020687 120 24 4.211178721656033 120 25 3.625067884874923 120 26 4.968620961973038 120 27 5.293662477050596 120 28 3.334572760135103 120 29 3.270171664850462 120 30 3.0735668561977825 120 31 1.7401329526724147 120 32 1.8742497019069582 120 33 2.7165519956328197 120 34 1.3374514455276092 120 35 2.2018891226024913 120 36 2.2851877680557244 120 37 3.075152722313014 120 38 -0.21512579994879033 120 39 1.5523196820018335 120 40 1.1013660977692261 120 41 -9.703192582871788 120 42 -9.316717201826817 120 43 -13.955329925350211 120 44 -17.6512092982847 120 45 -13.560955491980513 120 46 -13.894204917376959 120 47 -11.497555051076136 120 48 -12.136784029048952 120 49 -15.399307449555138 120 50 -11.576968367938296 120 51 -8.450528630302353 120 52 -8.290826421878492 120 53 -7.056408713501591 120 54 -9.272237129966296 120 55 -7.152984530488228 120 56 -5.931206907460407 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 232.0 5 37.0 6 46.0 7 78.0 8 91.0 9 119.0 10 87.0 11 68.0 12 46.0 13 51.0 14 48.0 15 63.0 16 61.0 17 106.0 18 99.0 19 163.0 20 184.0 21 266.0 22 322.0 23 392.0 24 418.0 25 479.0 26 512.0 27 542.0 28 555.0 29 637.0 30 666.0 31 808.0 32 877.0 33 962.0 34 1030.0 35 1092.0 36 1255.0 37 1366.0 38 1282.0 39 1000.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 0.0 100.0 0.0 0.0 3 0.0 0.0 100.0 0.0 4 0.0 0.0 0.0 100.0 5 0.0 0.0 0.0 100.0 6 0.0 0.0 0.0 100.0 7 98.46009975062344 1.1034912718204488 0.12468827930174563 0.3117206982543641 8 98.99002493765586 0.511221945137157 0.2057356608478803 0.29301745635910226 9 98.02992518703242 1.0286783042394014 0.4488778054862843 0.49251870324189523 10 64.91271820448877 20.542394014962593 4.276807980049875 10.268079800498754 11 46.34663341645886 21.07231920199501 19.002493765586035 13.578553615960102 12 37.281795511221944 13.291770573566083 21.3216957605985 28.104738154613468 13 28.57231920199501 13.884039900249375 26.078553615960097 31.465087281795512 14 25.81047381546135 23.709476309226932 25.280548628428928 25.199501246882793 15 25.200300450676018 23.00951427140711 24.480470706059087 27.309714571857786 16 32.36651696606786 21.20134730538922 23.833582834331338 22.598552894211576 17 32.36247973057254 19.701883497567668 24.522888861170014 23.412747910689784 18 27.331670822942645 21.60847880299252 26.10349127182045 24.956359102244388 19 30.940053646060754 22.87443079034371 20.915725781298732 25.269789782296797 20 31.841168758194417 22.032840107385905 22.270088031466567 23.855903102953114 21 30.374064837905234 21.90149625935162 23.30423940149626 24.420199501246884 22 23.94638403990025 21.795511221945137 28.372817955112218 25.885286783042392 23 22.43142144638404 24.831670822942645 25.15586034912718 27.581047381546135 24 22.1473999251777 23.400673400673398 25.65781269484973 28.794113979299162 25 24.40925244716005 28.380821746991707 22.757029740008726 24.452896065839518 26 27.35607808894156 26.7448387700368 21.56801596706792 24.33106717395372 27 24.716086359665542 22.806689130163484 22.619493323349555 29.857731186821418 28 23.721945137157107 20.38653366583541 26.826683291770575 29.064837905236907 29 27.72159800249688 22.965043695380775 19.694132334581774 29.619225967540576 30 30.233283433133735 21.307385229540916 23.98328343313373 24.476047904191617 31 30.691525846480015 23.115295878281476 23.034233335411862 23.15894493982665 32 30.53754053379895 23.22898478423547 22.68021950611125 23.55325517585433 33 29.57113427804482 23.04138835133279 22.80417004806792 24.583307322554468 34 27.566824881339 24.03197601798651 25.54334249313015 22.857856607544342 35 27.340286017610694 25.416848810341598 25.910197964154126 21.332667207893586 36 28.985778443113773 23.75249500998004 23.958333333333336 23.303393213572853 37 27.154634024481638 23.850861853609793 23.74469148138896 25.249812640519607 38 28.66516685414323 25.565554305711785 22.028496437945257 23.740782402199727 39 27.526371637226138 25.44160788964484 21.22214593346233 25.809874539666687 40 23.106869270375487 19.578048474193142 20.871530830089164 36.44355142534221 41 21.154330312185294 16.08761329305136 18.97029204431017 43.78776435045317 42 25.08909034073148 24.338855892466395 24.326351984995313 26.24570178180681 43 22.341621891790577 19.067849556416345 26.39635136823691 32.194177183556164 44 25.940532081377153 21.646322378716746 22.97339593114241 29.439749608763695 45 25.773389163177303 22.34235360289982 24.310980563714768 27.573276670208113 46 23.874437218609305 25.80665332666333 25.86293146573287 24.455977988994498 47 19.808366733466933 21.53682364729459 26.77855711422846 31.876252505010022 48 24.251310207137507 21.9241327676566 26.859246318941853 26.965310706264038 49 23.29272102467979 21.118400499843798 24.87347703842549 30.71540143705092 50 25.80584707646177 22.51374312843578 27.2863568215892 24.394052973513244 51 26.17558779389695 22.848924462231114 28.332916458229114 22.64257128564282 52 25.3544881004435 23.98026110312949 25.34824161409207 25.31700918233494 53 26.68209104552276 20.92296148074037 24.63106553276638 27.763881940970485 54 26.70749234518528 21.48347184902831 26.307567331125416 25.501468474661 55 24.62770616944062 23.3012138655988 26.848955074458765 25.222124890501814 56 23.879197148752578 22.891264928406176 28.16232101544426 25.06721690739699 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 1.5 9 3.0 10 2.0 11 1.0 12 2.0 13 4.0 14 5.0 15 10.0 16 15.0 17 13.0 18 11.0 19 11.5 20 12.0 21 14.5 22 15.0 23 13.0 24 16.0 25 19.0 26 23.5 27 28.0 28 31.0 29 41.5 30 49.0 31 48.5 32 48.0 33 75.0 34 102.0 35 125.5 36 149.0 37 163.0 38 195.0 39 213.0 40 226.5 41 240.0 42 278.5 43 317.0 44 360.5 45 404.0 46 492.5 47 686.0 48 791.0 49 811.0 50 831.0 51 789.0 52 747.0 53 813.0 54 928.5 55 978.0 56 993.5 57 1009.0 58 995.0 59 981.0 60 1059.0 61 1137.0 62 1237.5 63 1221.5 64 1105.0 65 1006.5 66 908.0 67 887.5 68 867.0 69 717.5 70 568.0 71 532.0 72 416.0 73 336.0 74 322.0 75 308.0 76 221.0 77 134.0 78 94.0 79 48.0 80 42.0 81 53.5 82 65.0 83 43.0 84 21.0 85 12.0 86 3.0 87 2.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.399002493765586 16 0.04987531172069825 17 0.03740648379052369 18 0.0 19 0.05610972568578554 20 0.1433915211970075 21 0.0 22 0.0 23 0.0 24 0.012468827930174562 25 0.006234413965087281 26 0.043640897755610975 27 0.08728179551122195 28 0.0 29 0.12468827930174563 30 0.04987531172069825 31 0.018703241895261846 32 0.024937655860349125 33 0.13092269326683292 34 0.1745635910224439 35 0.16832917705735662 36 0.04987531172069825 37 0.1745635910224439 38 0.2369077306733167 39 0.11845386533665835 40 0.7107231920199502 41 0.9476309226932668 42 0.2805486284289277 43 0.2119700748129676 44 0.40523690773067333 45 0.243142144638404 46 0.29925187032418954 47 0.4488778054862843 48 0.07481296758104738 49 0.21820448877805484 50 0.199501246882793 51 0.29925187032418954 52 0.19326683291770574 53 0.29925187032418954 54 0.23067331670822944 55 0.36159600997506236 56 0.29301745635910226 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 16040.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.92019950124688 #Duplication Level Percentage of deduplicated Percentage of total 1 89.17166477549036 68.59102244389027 2 6.540768357918624 10.062344139650872 3 1.766899011184957 4.077306733167083 4 0.8996595882639001 2.7680798004987532 5 0.4214621494569622 1.6209476309226933 6 0.3079915707570109 1.4214463840399003 7 0.17020586804992707 0.9164588528678304 8 0.09726049602852974 0.5985037406483791 9 0.04863024801426487 0.33665835411471323 >10 0.5430377694926244 7.874064837905237 >50 0.03242016534284325 1.7331670822942644 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CATCCCGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT 96 0.5985037406483791 No Hit CATCCCGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC 65 0.40523690773067333 No Hit CATCCCGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC 61 0.3802992518703242 No Hit CATCCCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC 56 0.3491271820448878 No Hit CATCCCGGGGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGC 50 0.3117206982543641 No Hit CATCCCGGGGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAA 47 0.29301745635910226 No Hit CATCCCGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATG 45 0.2805486284289277 No Hit CATCCCGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCC 39 0.243142144638404 No Hit CATCCCGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC 33 0.2057356608478803 No Hit CATCCCGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT 33 0.2057356608478803 No Hit CATCCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 33 0.2057356608478803 No Hit CATCCCGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA 32 0.199501246882793 No Hit CATCCCGGGGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTAC 32 0.199501246882793 No Hit CATCCCGGGGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAG 30 0.18703241895261846 No Hit CATCCCGGGGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGT 30 0.18703241895261846 No Hit CATCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 29 0.18079800498753118 No Hit CATCCCGGGATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGG 27 0.16832917705735662 No Hit CATCCCGGGGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCAT 26 0.1620947630922693 No Hit CATCCCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGACCTAATACATGCCGAC 24 0.14962593516209477 No Hit CATCCCGGGCTCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCG 22 0.1371571072319202 No Hit CATCCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 22 0.1371571072319202 No Hit CATCCCGGGACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTT 22 0.1371571072319202 No Hit CATCCCGGGGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTT 22 0.1371571072319202 No Hit CATCCCGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACCACCCATTCGAACGTC 22 0.1371571072319202 No Hit CATCCCGGGAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTG 21 0.13092269326683292 No Hit CATCCCGGGGGGGAGGTAGTGACGAAAAATAACAATACAGCACTCTTTCGAGGCCC 21 0.13092269326683292 No Hit CATCCCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGCGCTAATACATGCCGAC 20 0.12468827930174563 No Hit CATCCCGGGGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGA 20 0.12468827930174563 No Hit CATCCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 19 0.11845386533665835 No Hit CATCCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT 19 0.11845386533665835 No Hit CATCCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGT 18 0.11221945137157108 No Hit CATCCCGGGGACGCTACTCAGACTACCCAGATGCTTACACCACATGAAACACTGTC 18 0.11221945137157108 No Hit CATCCCGGGGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATG 18 0.11221945137157108 No Hit CATCCCGGGAAGGAGGGTTAGGATTGCTCCTTTTCCTTTGCTTCCAGTTATGGTGA 17 0.1059850374064838 No Hit CATCCCGGGATCAGATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGG 17 0.1059850374064838 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.006234413965087282 0.0 0.0 0.0 0.0 14 0.012468827930174564 0.0 0.0 0.0 0.0 15 0.012468827930174564 0.0 0.0 0.0 0.0 16 0.012468827930174564 0.0 0.0 0.0 0.0 17 0.012468827930174564 0.0 0.0 0.0 0.0 18 0.02493765586034913 0.0 0.0 0.0 0.0 19 0.02493765586034913 0.0 0.0 0.0 0.0 20 0.02493765586034913 0.0 0.0 0.0 0.0 21 0.02493765586034913 0.0 0.0 0.0 0.0 22 0.02493765586034913 0.0 0.0 0.0 0.0 23 0.03117206982543641 0.0 0.0 0.0 0.0 24 0.03117206982543641 0.0 0.0 0.0 0.0 25 0.03740648379052369 0.0 0.0 0.0 0.0 26 0.03740648379052369 0.0 0.0 0.0 0.0 27 0.03740648379052369 0.0 0.0 0.0 0.0 28 0.03740648379052369 0.0 0.0 0.0 0.0 29 0.03740648379052369 0.0 0.0 0.0 0.0 30 0.03740648379052369 0.0 0.0 0.0 0.0 31 0.03740648379052369 0.0 0.0 0.0 0.0 32 0.043640897755610975 0.0 0.0 0.0 0.0 33 0.043640897755610975 0.0 0.0 0.0 0.0 34 0.043640897755610975 0.0 0.0 0.0 0.0 35 0.043640897755610975 0.0 0.0 0.0 0.0 36 0.04987531172069826 0.0 0.0 0.0 0.0 37 0.04987531172069826 0.0 0.0 0.0 0.0 38 0.04987531172069826 0.0 0.0 0.0 0.0 39 0.05610972568578554 0.0 0.0 0.0 0.0 40 0.06234413965087282 0.0 0.0 0.0 0.0 41 0.06234413965087282 0.0 0.0 0.0 0.0 42 0.08104738154613467 0.0 0.0 0.0 0.0 43 0.08104738154613467 0.0 0.0 0.0 0.0 44 0.08104738154613467 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCAATCC 20 3.743128E-4 50.82428 41 AATCCGC 15 0.0078582745 50.66242 43 TTCAATC 15 0.0078582745 50.66242 40 CAATCCG 15 0.0078582745 50.66242 42 TCCGCCC 15 0.0078582745 50.66242 45 ATCCGCC 15 0.0078582745 50.66242 44 CCGCCCA 15 0.007958147 50.501587 46 GTTCAAT 15 0.007958147 50.501587 39 CGCCCAA 15 0.008160722 50.18296 47 ACAGATC 20 4.0496237E-4 50.025158 12 GCCCAAT 15 0.008263436 50.025158 48 TTATCAG 20 4.0496237E-4 50.025158 48 GTTTTTG 25 1.9892734E-5 50.025158 33 GAGTTCC 15 0.008263436 50.025158 10 CCCAATC 15 0.008263436 50.025158 49 GATCCTC 25 1.9892734E-5 50.025158 15 TATCAGA 20 4.0496237E-4 50.025158 49 CAGATCC 20 4.0496237E-4 50.025158 13 GCTCCTA 35 4.841786E-8 50.025158 9 AGATCCT 15 0.008263436 50.025158 14 >>END_MODULE