FastQCFastQC Report
Fri 17 Jun 2016
SRR1525941_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525941_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1797
Sequences flagged as poor quality0
Sequence length40
%GC60

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC160.8903728436282694No Hit
CATCCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG80.4451864218141347No Hit
CATCCCGGGGGGCTGGTGCGATGGCTCAGTGGGTAAGAGC80.4451864218141347No Hit
CATCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC70.38953811908736785No Hit
CATCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG70.38953811908736785No Hit
CATCCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCC60.333889816360601No Hit
CATCCCGGGGGGGCTGGTGAGCTGGCTCAGTGGGTAAGAG50.27824151363383415No Hit
CATCCCGGGGGGCTGGTGCGATGGCTCAGTGGGTAAGCGC50.27824151363383415No Hit
CATCCCGGGGGGGTTGGGGATTTAGCTCCGTGGTAGAGCG50.27824151363383415No Hit
CATCCCGGGGGGCTGGTGAGATGGCTCCGTGGGTAAGAGC40.22259321090706735No Hit
CATCCCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT40.22259321090706735No Hit
CATCCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGCGCC40.22259321090706735No Hit
CATCCCGGGGGGGTTGGGGATTTAGCTCCGTGGTAGCGCC30.1669449081803005No Hit
CATCCCGGGGGGGCTGGTGAGATGGCTCCGTGGGTAAGAG30.1669449081803005No Hit
CATCCCGGGGGGCTGGTGAGACGGCTCCGTGGGTAAGAGC30.1669449081803005No Hit
CATCCCGGGGTCAAAGAAGCTAAATTTATTTCGTGAAAAT30.1669449081803005No Hit
CATCCCGGGGGGGGTTGGGGATTTAGCTCCGTGGTAGAGC30.1669449081803005No Hit
CATCCCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGCGC30.1669449081803005No Hit
CATCCCGGGGGGCTGGTGCGATGGCTCCGTGGGTAAGAGC20.11129660545353368No Hit
CATCCCGGGGGGCTGGTGAGATGGCTCAGTGGGTCAGAGC20.11129660545353368No Hit
CATCCCGGGGGTACCAAAGTGCACTCTCTTCCGGTGCCTC20.11129660545353368No Hit
CATCCCGGGGGGGCTGGTGAGATGGCTCCGTGGGTAAGCG20.11129660545353368No Hit
CATCCCGGGGGGGCTGGTGAGCTGGCTCAGTGGGTAACAG20.11129660545353368No Hit
CATCCCGGGGGGTAAAATCAAAACCGGCTCCCCCTTTCTT20.11129660545353368No Hit
CATCCCGGGGGGGCTGGTGAGATGGCTCAGCAGTTAAGAG20.11129660545353368No Hit
CATCCCGGGGGGCTGGTGCGATGGCTCCGTGGGTACGAGC20.11129660545353368No Hit
CATCCCGGGCTCATTTTTTTTTTTTTTTTTTTTTTTTTTT20.11129660545353368No Hit
CATCCCGGGGGTGAAAAGCCTAACGAGCTTGGTGATAGCT20.11129660545353368No Hit
CATCCCGGGAGCTATAACTCTTATGGGTCCTCAGTGGTGG20.11129660545353368No Hit
CATCCCGGGGGGGTTGGGGATTTAGCTCAGTGGTACAGCC20.11129660545353368No Hit
CATCCCGGGGGGGCTGGTCAGATGGCTCAGTGGGTAAGAG20.11129660545353368No Hit
CATCCCGGGGGGCTGGTGAGATGGCTCAGTGGGTCCGCGC20.11129660545353368No Hit
CATCCCGGGCACAGCTTTACTCTTTCAGGTCACTCTGGGG20.11129660545353368No Hit
CATCCCGGGGGGGTTGGGGATTTAGCTCAGTGGTCAAGTG20.11129660545353368No Hit
CATCCCGGGGGGCTGGTGCGATGGCTCCGTGGGTAAGCGC20.11129660545353368No Hit
CATCCCGGGGGGGTTGGGGATTTAGCTCAGTGGTCGAGCG20.11129660545353368No Hit
CATCCCGGGGTCTCACACATGCTCTATATAAATGGGAAAT20.11129660545353368No Hit
CATCCCGGGGGGGCTGGTGAGATGGCTCCGTGGGTACGCG20.11129660545353368No Hit
CATCCCGGGGGGGTTGGGGATTTCGCTCAGTGGTAGACCG20.11129660545353368No Hit
CATCCCGGGGGGGCTGGTGAGATGGCTCCGTGGGTACGAG20.11129660545353368No Hit
CATCCCGGGGCAATAAAGCAGCAGCGGCCTTAGGTGCTTG20.11129660545353368No Hit
CATCCCGGGCTCTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.11129660545353368No Hit
CATCCCGGGGGGGCTGGTGAGATGGCCCAGTGGGTAAGAG20.11129660545353368No Hit
CATCCCGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.11129660545353368No Hit
CATCCCGGGGGGCTGGTGAGATGGCTCCGTGGGTACGAGC20.11129660545353368No Hit
CATCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGCG20.11129660545353368No Hit
CATCCCGGGGGGGTTGGGGATTTAGCTCCGTGGTCGCGCG20.11129660545353368No Hit
CATCCCGGGGGGCTGGTGCGATGGCTCCGTGGGTACGCGC20.11129660545353368No Hit
CATCCCGGGGGGCAGGTGGACGTCAGCTTCAGCTCTTGCG20.11129660545353368No Hit
CATCCCGGGTACGAGAGTTTTTTTTTTTTTTTTTTTTTTT20.11129660545353368No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCCGG1750.034.02
CGGGGGG451.549779E-934.06
CCCGGGA308.064844E-634.04
CGGGGAG200.002098324734.06
CATCCCG1750.034.01
CTGGTGC200.002098324734.013
CCGGGGC200.002098324734.05
CCGGGGA251.318037E-434.05
TCCCGGG1700.034.03
TGGTGCG200.002098324734.014
GGTGCGA200.002098324734.015
CCCGGGG1250.033.9999964
CCGGGGG700.031.5714285
GCTGGTG359.517446E-424.28571312
GGCTGGT359.517446E-424.28571311
GGGGGGC359.517446E-424.2857137
GGGGGCT359.517446E-424.2857138
GGGGCTG400.002076732521.259
GGGCTGG400.002076732521.2510
Produced by FastQC (version 0.11.5)