##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1525941_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1797 Sequences flagged as poor quality 0 Sequence length 40 %GC 60 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.36727879799666 33.0 31.0 35.0 27.0 37.0 2 16.707846410684475 16.0 10.0 23.0 4.0 29.0 3 25.711741791875347 31.0 17.0 35.0 4.0 37.0 4 23.85253199777407 28.0 15.0 34.0 4.0 36.0 5 24.45854201446856 31.0 15.0 34.0 4.0 36.0 6 17.700055648302726 18.0 4.0 31.0 4.0 33.0 7 13.387868670005565 6.0 4.0 23.0 4.0 29.0 8 13.029493600445186 4.0 4.0 22.0 4.0 28.0 9 13.678909293266555 4.0 4.0 24.0 4.0 30.0 10 10.45575959933222 4.0 4.0 16.0 4.0 24.0 11 14.739009460211463 4.0 4.0 28.0 4.0 33.0 12 13.969393433500278 4.0 4.0 26.0 4.0 31.0 13 13.580411797440178 4.0 4.0 26.0 4.0 31.0 14 13.82582081246522 4.0 4.0 26.0 4.0 32.0 15 13.385642737896495 4.0 4.0 26.0 4.0 33.0 16 12.649972175848637 4.0 4.0 25.0 4.0 32.0 17 12.204785754034502 4.0 4.0 24.0 4.0 32.0 18 11.766833611574848 4.0 4.0 22.0 4.0 32.0 19 10.589872008903729 4.0 4.0 16.0 4.0 31.0 20 10.810239287701725 4.0 4.0 19.0 4.0 32.0 21 8.99053978853645 4.0 4.0 4.0 4.0 28.0 22 9.151919866444073 4.0 4.0 4.0 4.0 29.0 23 8.757373400111296 4.0 4.0 4.0 4.0 28.0 24 8.535893155258764 4.0 4.0 4.0 4.0 29.0 25 8.35781858653311 4.0 4.0 4.0 4.0 29.0 26 7.175292153589315 4.0 4.0 4.0 4.0 21.0 27 7.165831942125765 4.0 4.0 4.0 4.0 22.0 28 6.800222593210907 4.0 4.0 4.0 4.0 19.0 29 6.470784641068447 4.0 4.0 4.0 4.0 13.0 30 6.149137451307735 4.0 4.0 4.0 4.0 9.0 31 6.004451864218141 4.0 4.0 4.0 4.0 4.0 32 5.893711741791876 4.0 4.0 4.0 4.0 4.0 33 5.796883695047301 4.0 4.0 4.0 4.0 4.0 34 5.78742348358375 4.0 4.0 4.0 4.0 4.0 35 5.7084028937117415 4.0 4.0 4.0 4.0 4.0 36 5.61825264329438 4.0 4.0 4.0 4.0 4.0 37 5.488592097941013 4.0 4.0 4.0 4.0 4.0 38 5.488035614913745 4.0 4.0 4.0 4.0 4.0 39 5.472454090150251 4.0 4.0 4.0 4.0 4.0 40 5.337228714524207 4.0 4.0 4.0 4.0 4.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -6.555102040816326 1 2 2.5469387755102026 1 3 -2.9790816326530596 1 4 -0.8093537414965972 1 5 -2.216156462585033 1 6 1.8375850340136068 1 7 10.213775510204083 1 8 6.8125850340136065 1 9 10.686224489795917 1 10 12.368197278911564 1 11 3.0559523809523785 1 12 11.213435374149661 1 13 6.039115646258505 1 14 10.74115646258503 1 15 9.766496598639453 1 16 13.973979591836732 1 17 11.224829931972787 1 18 13.522619047619047 1 19 -1.3663265306122456 1 20 15.436564625850341 1 21 4.7505102040816345 1 22 5.55187074829932 1 23 4.553571428571429 1 24 6.23469387755102 1 25 -3.321428571428571 1 26 -2.4974489795918364 1 27 -2.3069727891156466 1 28 -2.2814625850340136 1 29 -1.9523809523809526 1 30 -1.6598639455782322 1 31 -1.5238095238095228 1 32 -1.4387755102040813 1 33 -1.286564625850339 1 34 -1.3222789115646254 1 35 -0.9804421768707483 1 36 -1.3188775510204085 1 37 -1.137755102040816 1 38 -1.275510204081633 1 39 -0.9311224489795915 1 40 -0.8188775510204085 5 1 -1.221768707482994 5 2 -13.119727891156465 5 3 -22.97908163265306 5 4 -20.14268707482993 5 5 -20.216156462585033 5 6 -12.162414965986393 5 7 -7.452891156462584 5 8 -7.1874149659863935 5 9 -7.647108843537415 5 10 -5.631802721088436 5 11 -8.277380952380955 5 12 -8.119897959183671 5 13 -7.627551020408163 5 14 -7.592176870748302 5 15 -6.566836734693879 5 16 -6.3593537414966 5 17 -6.1085034013605455 5 18 -5.810714285714285 5 19 -5.032993197278913 5 20 -5.563435374149659 5 21 -3.5828231292516994 5 22 -3.7814625850340136 5 23 -3.446428571428571 5 24 -3.4319727891156457 5 25 -3.321428571428571 5 26 -2.4974489795918364 5 27 -2.3069727891156466 5 28 -2.2814625850340136 5 29 -1.9523809523809526 5 30 -1.6598639455782322 5 31 -1.5238095238095228 5 32 -1.4387755102040813 5 33 -1.286564625850339 5 34 -1.3222789115646254 5 35 -0.9804421768707483 5 36 -1.3188775510204085 5 37 -1.137755102040816 5 38 -1.275510204081633 5 39 -0.9311224489795915 5 40 -0.8188775510204085 6 1 5.778231292517006 6 2 11.880272108843535 6 3 9.02091836734694 6 4 4.857312925170071 6 5 12.783843537414967 6 6 19.837585034013607 6 7 18.547108843537416 6 8 19.81258503401361 6 9 18.352891156462583 6 10 13.368197278911564 6 11 25.722619047619045 6 12 20.88010204081633 6 13 20.372448979591837 6 14 26.407823129251696 6 15 24.43316326530612 6 16 25.640646258503402 6 17 23.891496598639456 6 18 19.189285714285717 6 19 14.967006802721087 6 20 24.43656462585034 6 21 -3.5828231292516994 6 22 -3.7814625850340136 6 23 -3.446428571428571 6 24 -3.4319727891156457 6 25 -3.321428571428571 6 26 -2.4974489795918364 6 27 -2.3069727891156466 6 28 -2.2814625850340136 6 29 -1.9523809523809526 6 30 -1.6598639455782322 6 31 -1.5238095238095228 6 32 -1.4387755102040813 6 33 -1.286564625850339 6 34 -1.3222789115646254 6 35 -0.9804421768707483 6 36 -1.3188775510204085 6 37 -1.137755102040816 6 38 -1.275510204081633 6 39 -0.9311224489795915 6 40 -0.8188775510204085 7 1 2.278231292517006 7 2 -8.9530612244898 7 3 -8.97908163265306 7 4 -15.64268707482993 7 5 -12.049489795918367 7 6 -7.162414965986393 7 7 -3.2862244897959183 7 8 -1.3540816326530596 7 9 -1.8137755102040813 7 10 -2.1318027210884356 7 11 1.2226190476190446 7 12 1.880102040816329 7 13 1.2057823129251712 7 14 2.074489795918364 7 15 3.0998299319727867 7 16 -0.3593537414965997 7 17 -0.6085034013605455 7 18 3.522619047619049 7 19 -5.032993197278913 7 20 -5.563435374149659 7 21 -3.5828231292516994 7 22 -3.7814625850340136 7 23 -3.446428571428571 7 24 -3.4319727891156457 7 25 -3.321428571428571 7 26 -2.4974489795918364 7 27 -2.3069727891156466 7 28 -2.2814625850340136 7 29 -1.9523809523809526 7 30 -1.6598639455782322 7 31 -1.5238095238095228 7 32 -1.4387755102040813 7 33 -1.286564625850339 7 34 -1.3222789115646254 7 35 -0.9804421768707483 7 36 -1.3188775510204085 7 37 -1.137755102040816 7 38 -1.275510204081633 7 39 -0.9311224489795915 7 40 -0.8188775510204085 8 1 1.778231292517006 8 2 0.8802721088435348 8 3 6.02091836734694 8 4 -20.14268707482993 8 5 -20.216156462585033 8 6 -12.162414965986393 8 7 -7.452891156462584 8 8 -7.1874149659863935 8 9 -7.647108843537415 8 10 -5.631802721088436 8 11 -8.277380952380955 8 12 -8.119897959183671 8 13 -7.627551020408163 8 14 -7.592176870748302 8 15 -6.566836734693879 8 16 -6.3593537414966 8 17 -6.1085034013605455 8 18 -5.810714285714285 8 19 -5.032993197278913 8 20 -5.563435374149659 8 21 -3.5828231292516994 8 22 -3.7814625850340136 8 23 -3.446428571428571 8 24 -3.4319727891156457 8 25 -3.321428571428571 8 26 -2.4974489795918364 8 27 -2.3069727891156466 8 28 -2.2814625850340136 8 29 -1.9523809523809526 8 30 -1.6598639455782322 8 31 -1.5238095238095228 8 32 -1.4387755102040813 8 33 -1.286564625850339 8 34 -1.3222789115646254 8 35 -0.9804421768707483 8 36 -1.3188775510204085 8 37 -1.137755102040816 8 38 -1.275510204081633 8 39 -0.9311224489795915 8 40 -0.8188775510204085 10 1 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2.4448979591836704 12 2 -6.453061224489799 12 3 -0.31241496598639173 12 4 1.1906462585034028 12 5 10.117176870748303 12 6 -1.8290816326530592 12 7 -7.452891156462584 12 8 -7.1874149659863935 12 9 -7.647108843537415 12 10 -5.631802721088436 12 11 -8.277380952380955 12 12 -8.119897959183671 12 13 -7.627551020408163 12 14 -7.592176870748302 12 15 -6.566836734693879 12 16 -6.3593537414966 12 17 -6.1085034013605455 12 18 -5.810714285714285 12 19 -5.032993197278913 12 20 -5.563435374149659 12 21 -3.5828231292516994 12 22 -3.7814625850340136 12 23 -3.446428571428571 12 24 -3.4319727891156457 12 25 -3.321428571428571 12 26 -2.4974489795918364 12 27 -2.3069727891156466 12 28 -2.2814625850340136 12 29 -1.9523809523809526 12 30 -1.6598639455782322 12 31 -1.5238095238095228 12 32 -1.4387755102040813 12 33 -1.286564625850339 12 34 -1.3222789115646254 12 35 -0.9804421768707483 12 36 -1.3188775510204085 12 37 -1.137755102040816 12 38 -1.275510204081633 12 39 -0.9311224489795915 12 40 -0.8188775510204085 13 1 -0.22176870748299393 13 2 12.880272108843535 13 3 -11.97908163265306 13 4 -11.14268707482993 13 5 -9.216156462585033 13 6 14.837585034013607 13 7 19.547108843537416 13 8 21.81258503401361 13 9 16.352891156462583 13 10 12.368197278911564 13 11 20.722619047619045 13 12 25.88010204081633 13 13 26.372448979591837 13 14 26.407823129251696 13 15 27.43316326530612 13 16 27.640646258503402 13 17 27.891496598639456 13 18 28.189285714285717 13 19 17.967006802721087 13 20 28.43656462585034 13 21 28.4171768707483 13 22 26.218537414965986 13 23 28.55357142857143 13 24 24.568027210884352 13 25 28.67857142857143 13 26 23.502551020408163 13 27 23.693027210884352 13 28 14.718537414965986 13 29 24.047619047619047 13 30 18.34013605442177 13 31 7.476190476190477 13 32 12.561224489795919 13 33 14.713435374149661 13 34 23.677721088435376 13 35 14.01955782312925 13 36 26.681122448979593 13 37 27.862244897959183 13 38 26.724489795918366 13 39 -0.9311224489795915 13 40 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40 -0.8188775510204085 17 1 1.4448979591836704 17 2 -0.45306122448979735 17 3 -9.97908163265306 17 4 4.857312925170071 17 5 -7.216156462585033 17 6 14.837585034013607 17 7 11.547108843537416 17 8 9.145918367346939 17 9 14.019557823129253 17 10 4.0348639455782305 17 11 21.722619047619045 17 12 11.880102040816329 17 13 17.039115646258505 17 14 15.074489795918366 17 15 8.099829931972788 17 16 6.973979591836732 17 17 11.224829931972787 17 18 10.189285714285715 17 19 10.633673469387755 17 20 10.436564625850341 17 21 14.083843537414968 17 22 16.218537414965986 17 23 17.55357142857143 17 24 15.234693877551022 17 25 8.011904761904763 17 26 0.16921768707483054 17 27 5.3596938775510194 17 28 6.718537414965986 17 29 7.7142857142857135 17 30 -1.6598639455782322 17 31 -1.5238095238095228 17 32 -1.4387755102040813 17 33 -1.286564625850339 17 34 -1.3222789115646254 17 35 -0.9804421768707483 17 36 -1.3188775510204085 17 37 -1.137755102040816 17 38 -1.275510204081633 17 39 -0.9311224489795915 17 40 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2.778231292517006 19 2 -6.119727891156465 19 3 5.02091836734694 19 4 5.857312925170071 19 5 7.783843537414967 19 6 6.837585034013607 19 7 8.547108843537416 19 8 7.8125850340136065 19 9 8.352891156462585 19 10 8.368197278911564 19 11 22.722619047619045 19 12 22.88010204081633 19 13 3.3724489795918373 19 14 20.407823129251696 19 15 -6.566836734693879 19 16 -6.3593537414966 19 17 -6.1085034013605455 19 18 -5.810714285714285 19 19 -5.032993197278913 19 20 -5.563435374149659 19 21 -3.5828231292516994 19 22 -3.7814625850340136 19 23 -3.446428571428571 19 24 -3.4319727891156457 19 25 -3.321428571428571 19 26 -2.4974489795918364 19 27 -2.3069727891156466 19 28 -2.2814625850340136 19 29 -1.9523809523809526 19 30 -1.6598639455782322 19 31 -1.5238095238095228 19 32 -1.4387755102040813 19 33 -1.286564625850339 19 34 -1.3222789115646254 19 35 -0.9804421768707483 19 36 -1.3188775510204085 19 37 -1.137755102040816 19 38 -1.275510204081633 19 39 -0.9311224489795915 19 40 -0.8188775510204085 20 1 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-7.1874149659863935 111 9 -7.647108843537415 111 10 -5.631802721088436 111 11 -8.277380952380955 111 12 -8.119897959183671 111 13 -7.627551020408163 111 14 -7.592176870748302 111 15 -6.566836734693879 111 16 -6.3593537414966 111 17 -6.1085034013605455 111 18 -5.810714285714285 111 19 -5.032993197278913 111 20 -5.563435374149659 111 21 -3.5828231292516994 111 22 -3.7814625850340136 111 23 -3.446428571428571 111 24 -3.4319727891156457 111 25 -3.321428571428571 111 26 -2.4974489795918364 111 27 -2.3069727891156466 111 28 -2.2814625850340136 111 29 -1.9523809523809526 111 30 -1.6598639455782322 111 31 -1.5238095238095228 111 32 -1.4387755102040813 111 33 -1.286564625850339 111 34 -1.3222789115646254 111 35 -0.9804421768707483 111 36 -1.3188775510204085 111 37 -1.137755102040816 111 38 -1.275510204081633 111 39 -0.9311224489795915 111 40 -0.8188775510204085 112 1 -7.888435374149662 112 2 -9.453061224489797 112 3 -13.312414965986394 112 4 -18.809353741496597 112 5 -12.549489795918367 112 6 -2.8290816326530592 112 7 -3.1195578231292505 112 8 -4.1874149659863935 112 9 0.35289115646258473 112 10 3.3681972789115644 112 11 -3.6107142857142893 112 12 -4.119897959183671 112 13 -1.2942176870748288 112 14 -0.9255102040816361 112 15 0.7664965986394545 112 16 3.3073129251700664 112 17 0.22482993197278844 112 18 -2.144047619047618 112 19 4.6336734693877535 112 20 -5.563435374149659 112 21 -3.5828231292516994 112 22 -3.7814625850340136 112 23 -3.446428571428571 112 24 -3.4319727891156457 112 25 -3.321428571428571 112 26 -2.4974489795918364 112 27 -2.3069727891156466 112 28 -2.2814625850340136 112 29 -1.9523809523809526 112 30 -1.6598639455782322 112 31 -1.5238095238095228 112 32 -1.4387755102040813 112 33 -1.286564625850339 112 34 -1.3222789115646254 112 35 -0.9804421768707483 112 36 -1.3188775510204085 112 37 -1.137755102040816 112 38 -1.275510204081633 112 39 -0.9311224489795915 112 40 -0.8188775510204085 114 1 -0.7217687074829939 114 2 1.8802721088435348 114 3 -4.47908163265306 114 4 4.357312925170071 114 5 -10.216156462585033 114 6 12.837585034013607 114 7 9.547108843537416 114 8 3.3125850340136065 114 9 12.352891156462585 114 10 11.368197278911564 114 11 12.722619047619045 114 12 19.38010204081633 114 13 16.872448979591837 114 14 15.407823129251698 114 15 18.43316326530612 114 16 17.640646258503402 114 17 17.891496598639456 114 18 13.689285714285715 114 19 11.467006802721087 114 20 15.936564625850341 114 21 15.9171768707483 114 22 17.718537414965986 114 23 19.55357142857143 114 24 23.068027210884352 114 25 19.17857142857143 114 26 5.002551020408164 114 27 -0.3069727891156466 114 28 4.218537414965986 114 29 2.5476190476190474 114 30 -0.15986394557823225 114 31 11.976190476190478 114 32 12.061224489795919 114 33 -1.286564625850339 114 34 -1.3222789115646254 114 35 -0.9804421768707483 114 36 -1.3188775510204085 114 37 -1.137755102040816 114 38 -1.275510204081633 114 39 -0.9311224489795915 114 40 -0.8188775510204085 116 1 -2.471768707482994 116 2 11.380272108843535 116 3 7.02091836734694 116 4 10.85731292517007 116 5 9.533843537414967 116 6 8.087585034013607 116 7 4.547108843537416 116 8 1.8125850340136065 116 9 7.102891156462585 116 10 4.118197278911564 116 11 5.222619047619045 116 12 3.880102040816329 116 13 7.122448979591837 116 14 8.907823129251698 116 15 8.93316326530612 116 16 7.1406462585034 116 17 7.8914965986394545 116 18 7.939285714285715 116 19 0.9670068027210874 116 20 2.686564625850341 116 21 3.4171768707483006 116 22 9.718537414965986 116 23 0.053571428571428825 116 24 7.568027210884354 116 25 -0.3214285714285712 116 26 1.0025510204081636 116 27 0.9430272108843534 116 28 0.9685374149659864 116 29 2.2976190476190474 116 30 -0.15986394557823225 116 31 3.976190476190477 116 32 4.061224489795919 116 33 4.963435374149661 116 34 4.177721088435375 116 35 5.519557823129252 116 36 6.4311224489795915 116 37 6.612244897959184 116 38 6.474489795918367 116 39 6.8188775510204085 116 40 7.1811224489795915 >>END_MODULE >>Per sequence quality scores fail #Quality Count 4 267.0 5 101.0 6 179.0 7 238.0 8 113.0 9 40.0 10 74.0 11 74.0 12 77.0 13 78.0 14 80.0 15 67.0 16 61.0 17 58.0 18 53.0 19 44.0 20 41.0 21 25.0 22 26.0 23 16.0 24 23.0 25 17.0 26 16.0 27 11.0 28 6.0 29 6.0 30 3.0 31 1.0 32 1.0 33 0.0 34 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 0.0 100.0 0.0 0.0 3 0.0 0.0 100.0 0.0 4 0.0 0.0 0.0 100.0 5 0.0 0.0 0.0 100.0 6 0.0 0.0 0.0 100.0 7 95.42920847268674 2.0624303232998886 0.8361204013377926 1.6722408026755853 8 95.49248747913188 0.8903728436282694 1.1129660545353366 2.5041736227045077 9 95.92861126603458 1.3385387618516453 0.9481316229782488 1.7847183491355272 10 67.00055648302727 19.922092376182526 5.45353366722315 7.623817473567056 11 50.75125208681135 16.193656093489146 18.308291597106287 14.746800222593212 12 49.860879243183085 9.237618252643294 20.03338898163606 20.868113522537563 13 35.55926544240401 13.745130773511407 21.758486366165833 28.937117417918756 14 21.202003338898166 17.306622148024488 34.891485809682806 26.599888703394548 15 28.380634390651082 20.812465219810797 32.33166388425153 18.47523650528659 16 42.34835837506956 18.64218141346689 21.92543127434613 17.084028937117417 17 34.72454090150251 18.030050083472453 26.099053978853643 21.146355036171396 18 37.78519755147468 19.36560934891486 22.815804117974402 20.03338898163606 19 28.076062639821032 17.953020134228186 17.449664429530202 36.52125279642058 20 32.49860879243183 29.215358931552586 20.58987200890373 17.696160267111853 21 28.547579298831387 22.982749026154703 27.212020033388985 21.257651641624932 22 19.755147468002228 18.47523650528659 35.78185865331107 25.98775737340011 23 27.490261547022815 18.64218141346689 33.3889816360601 20.478575403450193 24 33.889816360601 21.92543127434613 23.817473567056204 20.36727879799666 25 29.342984409799556 18.652561247216035 19.320712694877507 32.68374164810691 26 18.085106382978726 18.365061590145576 27.99552071668533 35.554311310190364 27 17.139677239844183 14.468558708959378 32.220367278797994 36.17139677239844 28 13.752783964365257 21.43652561247216 24.109131403118038 40.70155902004454 29 24.053452115812917 21.21380846325167 22.10467706013363 32.62806236080178 30 26.46239554317549 13.426183844011142 30.41782729805014 29.693593314763234 31 26.421404682274247 14.43701226309922 32.608695652173914 26.53288740245262 32 36.449638286032275 11.797440178074568 24.485253199777407 27.267668336115747 33 35.85746102449889 13.752783964365257 26.948775055679285 23.44097995545657 34 22.036727879799667 15.247634947134111 40.73455759599332 21.981079577072897 35 15.859766277128548 24.095715080690038 32.38731218697829 27.657206455203116 36 18.47523650528659 29.437952142459658 24.707846410684475 27.378964941569283 37 24.4988864142539 24.55456570155902 23.21826280623608 27.728285077951004 38 21.92543127434613 21.92543127434613 23.650528658875906 32.49860879243183 39 22.871452420701168 19.30996104618809 22.426265998887036 35.39232053422371 40 21.981079577072897 15.358931552587645 22.927100723427934 39.73288814691152 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.5 15 1.0 16 0.5 17 0.0 18 0.0 19 0.5 20 1.0 21 5.0 22 9.0 23 9.0 24 8.0 25 7.0 26 9.5 27 12.0 28 12.0 29 13.5 30 15.0 31 14.5 32 14.0 33 14.0 34 13.5 35 13.0 36 17.5 37 22.0 38 22.0 39 27.5 40 33.0 41 39.5 42 46.0 43 46.0 44 54.5 45 63.0 46 55.0 47 47.0 48 47.0 49 63.0 50 79.0 51 72.0 52 65.0 53 65.0 54 87.5 55 110.0 56 106.0 57 102.0 58 102.0 59 113.5 60 125.0 61 152.5 62 180.0 63 180.0 64 203.0 65 226.0 66 226.0 67 226.0 68 226.0 69 200.0 70 174.0 71 129.5 72 85.0 73 85.0 74 75.5 75 66.0 76 51.0 77 36.0 78 36.0 79 27.5 80 19.0 81 13.0 82 7.0 83 7.0 84 6.0 85 5.0 86 3.0 87 1.0 88 1.0 89 0.5 90 0.0 91 0.5 92 1.0 93 1.0 94 4.0 95 7.0 96 3.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.1669449081803005 8 0.0 9 0.22259321090706735 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.5008347245409015 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.05564830272676684 26 0.6121313299944352 27 0.0 28 0.05564830272676684 29 0.05564830272676684 30 0.11129660545353368 31 0.1669449081803005 32 0.0 33 0.05564830272676684 34 0.0 35 0.0 36 0.0 37 0.05564830272676684 38 0.0 39 0.0 40 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 40 1797.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 93.82303839732889 #Duplication Level Percentage of deduplicated Percentage of total 1 97.03440094899169 91.04062326099054 2 1.8979833926453145 3.5614913745130776 3 0.3558718861209964 1.001669449081803 4 0.1779359430604982 0.667779632721202 5 0.1779359430604982 0.8347245409015025 6 0.05931198102016608 0.333889816360601 7 0.11862396204033215 0.7790762381747357 8 0.11862396204033215 0.8903728436282694 9 0.0 0.0 >10 0.05931198102016608 0.8903728436282694 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CATCCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC 16 0.8903728436282694 No Hit CATCCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG 8 0.4451864218141347 No Hit CATCCCGGGGGGCTGGTGCGATGGCTCAGTGGGTAAGAGC 8 0.4451864218141347 No Hit CATCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC 7 0.38953811908736785 No Hit CATCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG 7 0.38953811908736785 No Hit CATCCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCC 6 0.333889816360601 No Hit CATCCCGGGGGGGCTGGTGAGCTGGCTCAGTGGGTAAGAG 5 0.27824151363383415 No Hit CATCCCGGGGGGCTGGTGCGATGGCTCAGTGGGTAAGCGC 5 0.27824151363383415 No Hit CATCCCGGGGGGGTTGGGGATTTAGCTCCGTGGTAGAGCG 5 0.27824151363383415 No Hit CATCCCGGGGGGCTGGTGAGATGGCTCCGTGGGTAAGAGC 4 0.22259321090706735 No Hit CATCCCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT 4 0.22259321090706735 No Hit CATCCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGCGCC 4 0.22259321090706735 No Hit CATCCCGGGGGGGTTGGGGATTTAGCTCCGTGGTAGCGCC 3 0.1669449081803005 No Hit CATCCCGGGGGGGCTGGTGAGATGGCTCCGTGGGTAAGAG 3 0.1669449081803005 No Hit CATCCCGGGGGGCTGGTGAGACGGCTCCGTGGGTAAGAGC 3 0.1669449081803005 No Hit CATCCCGGGGTCAAAGAAGCTAAATTTATTTCGTGAAAAT 3 0.1669449081803005 No Hit CATCCCGGGGGGGGTTGGGGATTTAGCTCCGTGGTAGAGC 3 0.1669449081803005 No Hit CATCCCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGCGC 3 0.1669449081803005 No Hit CATCCCGGGGGGCTGGTGCGATGGCTCCGTGGGTAAGAGC 2 0.11129660545353368 No Hit CATCCCGGGGGGCTGGTGAGATGGCTCAGTGGGTCAGAGC 2 0.11129660545353368 No Hit CATCCCGGGGGTACCAAAGTGCACTCTCTTCCGGTGCCTC 2 0.11129660545353368 No Hit CATCCCGGGGGGGCTGGTGAGATGGCTCCGTGGGTAAGCG 2 0.11129660545353368 No Hit CATCCCGGGGGGGCTGGTGAGCTGGCTCAGTGGGTAACAG 2 0.11129660545353368 No Hit CATCCCGGGGGGTAAAATCAAAACCGGCTCCCCCTTTCTT 2 0.11129660545353368 No Hit CATCCCGGGGGGGCTGGTGAGATGGCTCAGCAGTTAAGAG 2 0.11129660545353368 No Hit CATCCCGGGGGGCTGGTGCGATGGCTCCGTGGGTACGAGC 2 0.11129660545353368 No Hit CATCCCGGGCTCATTTTTTTTTTTTTTTTTTTTTTTTTTT 2 0.11129660545353368 No Hit CATCCCGGGGGTGAAAAGCCTAACGAGCTTGGTGATAGCT 2 0.11129660545353368 No Hit CATCCCGGGAGCTATAACTCTTATGGGTCCTCAGTGGTGG 2 0.11129660545353368 No Hit CATCCCGGGGGGGTTGGGGATTTAGCTCAGTGGTACAGCC 2 0.11129660545353368 No Hit CATCCCGGGGGGGCTGGTCAGATGGCTCAGTGGGTAAGAG 2 0.11129660545353368 No Hit CATCCCGGGGGGCTGGTGAGATGGCTCAGTGGGTCCGCGC 2 0.11129660545353368 No Hit CATCCCGGGCACAGCTTTACTCTTTCAGGTCACTCTGGGG 2 0.11129660545353368 No Hit CATCCCGGGGGGGTTGGGGATTTAGCTCAGTGGTCAAGTG 2 0.11129660545353368 No Hit CATCCCGGGGGGCTGGTGCGATGGCTCCGTGGGTAAGCGC 2 0.11129660545353368 No Hit CATCCCGGGGGGGTTGGGGATTTAGCTCAGTGGTCGAGCG 2 0.11129660545353368 No Hit CATCCCGGGGTCTCACACATGCTCTATATAAATGGGAAAT 2 0.11129660545353368 No Hit CATCCCGGGGGGGCTGGTGAGATGGCTCCGTGGGTACGCG 2 0.11129660545353368 No Hit CATCCCGGGGGGGTTGGGGATTTCGCTCAGTGGTAGACCG 2 0.11129660545353368 No Hit CATCCCGGGGGGGCTGGTGAGATGGCTCCGTGGGTACGAG 2 0.11129660545353368 No Hit CATCCCGGGGCAATAAAGCAGCAGCGGCCTTAGGTGCTTG 2 0.11129660545353368 No Hit CATCCCGGGCTCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2 0.11129660545353368 No Hit CATCCCGGGGGGGCTGGTGAGATGGCCCAGTGGGTAAGAG 2 0.11129660545353368 No Hit CATCCCGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2 0.11129660545353368 No Hit CATCCCGGGGGGCTGGTGAGATGGCTCCGTGGGTACGAGC 2 0.11129660545353368 No Hit CATCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGCG 2 0.11129660545353368 No Hit CATCCCGGGGGGGTTGGGGATTTAGCTCCGTGGTCGCGCG 2 0.11129660545353368 No Hit CATCCCGGGGGGCTGGTGCGATGGCTCCGTGGGTACGCGC 2 0.11129660545353368 No Hit CATCCCGGGGGGCAGGTGGACGTCAGCTTCAGCTCTTGCG 2 0.11129660545353368 No Hit CATCCCGGGTACGAGAGTTTTTTTTTTTTTTTTTTTTTTT 2 0.11129660545353368 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.0 0.0 17 0.0 0.0 0.0 0.0 0.0 18 0.0 0.0 0.0 0.0 0.0 19 0.0 0.0 0.0 0.0 0.0 20 0.0 0.0 0.0 0.0 0.0 21 0.0 0.0 0.0 0.0 0.0 22 0.0 0.0 0.0 0.0 0.0 23 0.0 0.0 0.0 0.0 0.0 24 0.0 0.0 0.0 0.0 0.0 25 0.0 0.0 0.0 0.0 0.0 26 0.0 0.0 0.0 0.0 0.0 27 0.0 0.0 0.0 0.0 0.0 28 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCCCGG 175 0.0 34.0 2 CGGGGGG 45 1.549779E-9 34.0 6 CCCGGGA 30 8.064844E-6 34.0 4 CGGGGAG 20 0.0020983247 34.0 6 CATCCCG 175 0.0 34.0 1 CTGGTGC 20 0.0020983247 34.0 13 CCGGGGC 20 0.0020983247 34.0 5 CCGGGGA 25 1.318037E-4 34.0 5 TCCCGGG 170 0.0 34.0 3 TGGTGCG 20 0.0020983247 34.0 14 GGTGCGA 20 0.0020983247 34.0 15 CCCGGGG 125 0.0 33.999996 4 CCGGGGG 70 0.0 31.571428 5 GCTGGTG 35 9.517446E-4 24.285713 12 GGCTGGT 35 9.517446E-4 24.285713 11 GGGGGGC 35 9.517446E-4 24.285713 7 GGGGGCT 35 9.517446E-4 24.285713 8 GGGGCTG 40 0.0020767325 21.25 9 GGGCTGG 40 0.0020767325 21.25 10 >>END_MODULE