FastQCFastQC Report
Fri 17 Jun 2016
SRR1525938_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525938_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84547
Sequences flagged as poor quality0
Sequence length56
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTGAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT8901.0526689297077365No Hit
ACCTGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG7550.892994429134091No Hit
ACCTGAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC7150.8456834660011591No Hit
ACCTGAGGGAGATTGGGCGGATTGAACAAAAACTCATCTCAGAAGAGGATCTGTGA6150.7274060581688292No Hit
ACCTGAAGGAGATTGGGCGGATTGAACAAAAACTCATCTCAGAAGAGGATCTGTGA4670.5523554945769809No Hit
ACCTGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC3700.4376264089796208No Hit
ACCTGAGGGAGATTGGGCGGATTGAACAAAAACTCATCTCCGAAGAGGATCTGTGA3450.4080570570215383No Hit
ACCTGAGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT3370.3985948643949519No Hit
ACCTGAGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC2750.32526287153890737No Hit
ACCTGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC2740.3240800974605841No Hit
ACCTGAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC2490.29451074550250156No Hit
ACCTGAGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT2350.2779519084059754No Hit
ACCTGAAGGAGATTGGGCGGATTGAACAAAAACTCATCTCCGAAGAGGATCTGTGA2240.26494139354441903No Hit
ACCTGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG1940.2294581711947201No Hit
ACCTGAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT1780.21053378594154729No Hit
ACCTGAGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC1760.20816823778490068No Hit
ACCTGAGGGGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCAT1460.17268501543520173No Hit
ACCTGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC1440.17031946727855513No Hit
ACCTGAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA1260.14902953386873571No Hit
ACCTGAGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA1240.14666398571208913No Hit
ACCTGAGGGATCAGATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGG1230.14548121163376582No Hit
ACCTGAGGGAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTG1100.13010514861556294No Hit
ACCTGAGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATG1100.13010514861556294No Hit
ACCTGAGGGGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGC1020.12064295598897654No Hit
ACCTGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG1010.11946018191065323No Hit
ACCTGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG1010.11946018191065323No Hit
ACCTGAGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC990.11709463375400664No Hit
ACCTGAGGGAACTGTGGTAATTCTAGAGCTAATACATGCCCACGGGCGCTGACCCC910.10763244112742025No Hit
ACCTGAGGGACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTT890.10526689297077364No Hit
ACCTGAGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGG890.10526689297077364No Hit
ACCTGAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCCGATTGAACAAAAACTC860.10171857073580375No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCCGC203.900272E-450.66084741
AGCCGAC150.00797863550.66084341
CCAATCC251.915882E-550.66084341
GTTTAAT203.935219E-450.56945439
CTACGAA150.00803583450.5694538
TACGAAG150.00803583450.5694539
ACTCGCC150.00803583450.5694539
TACTCGC150.00803583450.5694538
TCTACGA150.00805496850.5390637
TTGTTCG150.00805496850.5390637
GCGACCA150.00813184350.41786636
ATCTACG203.9940162E-450.41786636
TATGCTC251.971236E-550.41786635
TTAAGCG150.00813184350.41786636
ATGCCCA150.00813184350.41786636
ATATGCA150.00818985750.32734734
TAATCCG150.00822870350.26718542
GAAGACG354.996582E-850.2671842
AGACGAT402.5229383E-950.2371644
CCCCTAC252.0136156E-550.23715645
Produced by FastQC (version 0.11.5)