##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1525938_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 84547 Sequences flagged as poor quality 0 Sequence length 56 %GC 52 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.163885176292474 38.0 36.0 39.0 31.0 40.0 2 35.00497947886974 39.0 35.0 40.0 24.0 40.0 3 34.89405892580458 39.0 35.0 40.0 24.0 40.0 4 34.90466840928714 39.0 35.0 40.0 23.0 40.0 5 34.16955066412765 38.0 33.0 39.0 22.0 40.0 6 34.473488119034386 38.0 35.0 39.0 20.0 40.0 7 34.743586407560294 38.0 35.0 39.0 25.0 40.0 8 34.77417294522573 38.0 35.0 39.0 25.0 40.0 9 34.89111381834956 38.0 35.0 39.0 25.0 40.0 10 33.91378759743102 38.0 33.0 39.0 21.0 40.0 11 33.29013448141271 38.0 32.0 39.0 20.0 40.0 12 32.59738370373875 37.0 31.0 39.0 16.0 40.0 13 32.05423019149112 37.0 29.0 39.0 14.0 40.0 14 32.86722178196742 38.0 31.0 39.0 17.0 40.0 15 31.55078240505281 36.0 29.0 39.0 11.0 40.0 16 32.08339740026258 37.0 29.0 39.0 15.0 40.0 17 31.176907518894815 36.0 27.0 39.0 13.0 40.0 18 30.489656640685062 35.0 26.0 39.0 11.0 40.0 19 31.94167741019788 36.0 29.0 39.0 16.0 40.0 20 32.15973363927756 36.0 29.0 39.0 18.0 40.0 21 32.34240126793381 37.0 30.0 39.0 16.0 40.0 22 31.62665736217725 36.0 29.0 39.0 13.0 40.0 23 32.435438276934725 38.0 30.0 39.0 16.0 40.0 24 31.8254107182987 36.0 29.0 39.0 14.0 40.0 25 32.2023135060972 36.0 30.0 39.0 16.0 40.0 26 32.653293434421094 37.0 32.0 39.0 16.0 40.0 27 32.72644801116539 38.0 32.0 39.0 17.0 40.0 28 32.856292949483716 38.0 32.0 39.0 17.0 40.0 29 32.40384638130271 37.0 31.0 39.0 14.0 40.0 30 32.106047523862465 36.0 31.0 39.0 13.0 40.0 31 32.6655588016133 37.0 32.0 39.0 13.0 40.0 32 32.54769536470839 37.0 32.0 39.0 11.0 40.0 33 32.07501153204726 36.0 31.0 39.0 10.0 40.0 34 32.15036606857724 36.0 31.0 39.0 10.0 40.0 35 32.03182845044768 36.0 31.0 39.0 4.0 40.0 36 31.368493264101623 36.0 30.0 38.0 4.0 40.0 37 31.11560433841532 36.0 30.0 38.0 4.0 40.0 38 30.450010053579664 35.0 29.0 38.0 4.0 40.0 39 29.738843483506216 35.0 27.0 38.0 4.0 40.0 40 27.352703230156006 33.0 18.0 38.0 4.0 39.0 41 25.72642435568382 31.0 13.0 37.0 4.0 39.0 42 26.37722213679965 31.0 18.0 37.0 4.0 39.0 43 26.452245496587697 32.0 18.0 37.0 4.0 39.0 44 25.689628254107184 31.0 14.0 37.0 4.0 39.0 45 26.465622671413534 32.0 18.0 37.0 4.0 39.0 46 24.524749547588915 29.0 11.0 36.0 4.0 39.0 47 23.41527197889931 28.0 7.0 35.0 4.0 39.0 48 24.105266892970775 29.0 9.0 35.0 4.0 39.0 49 23.056725844796386 27.0 4.0 35.0 4.0 39.0 50 23.907483411593553 29.0 4.0 35.0 4.0 39.0 51 23.95397826061244 30.0 4.0 36.0 4.0 39.0 52 23.90476303121341 30.0 4.0 36.0 4.0 39.0 53 23.652110660342768 29.0 4.0 36.0 4.0 39.0 54 23.413072019113628 29.0 4.0 35.0 4.0 39.0 55 23.056903260908133 29.0 4.0 35.0 4.0 39.0 56 22.648952653553646 29.0 4.0 36.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 4.032283594404014 1 2 5.557200151298247 1 3 5.691557683162777 1 4 5.768413838350121 1 5 6.576391031251855 1 6 6.127028999887461 1 7 5.803216182862144 1 8 5.6180819859986215 1 9 5.457471636973615 1 10 6.235408549980566 1 11 0.7871573090312829 1 12 -5.516427000445585 1 13 -16.233289785963013 1 14 -5.095254864718395 1 15 2.4545329619819114 1 16 -0.632338014906459 1 17 -4.125646404836946 1 18 -3.747750768834795 1 19 0.3113185511652006 1 20 -0.5921584602730796 1 21 6.678367188602532 1 22 7.474631447811522 1 23 7.0484517829795195 1 24 7.217651636486362 1 25 6.106678755954611 1 26 6.2825198139233684 1 27 6.135878512089906 1 28 6.435852353474562 1 29 7.0346564535156055 1 30 6.877595418164262 1 31 5.13676662528847 1 32 5.68298799065203 1 33 5.351145268254804 1 34 4.262358940061919 1 35 4.647854796217846 1 36 3.61995527585227 1 37 4.236728628893747 1 38 4.317124271324179 1 39 4.013766630719385 1 40 -10.261551060894632 1 41 -1.4556054642785234 1 42 -3.3552143547921958 1 43 -8.830599772131364 1 44 -14.768960719016988 1 45 -3.8313536408239095 1 46 -6.42178676238653 1 47 -9.904569102361533 1 48 -5.825537975316379 1 49 -12.480035332003093 1 50 -5.2252328920743345 1 51 0.7208658889048323 1 52 0.3171604833416559 1 53 0.30767496471930755 1 54 0.5730225851278661 1 55 0.37352094988306916 1 56 2.3277222925692165 2 1 4.281229730235395 2 2 6.115279776590988 2 3 6.141674779181514 2 4 6.176259271605389 2 5 6.656367612048111 2 6 6.579956400355844 2 7 5.990452716819995 2 8 5.991735381783165 2 9 6.020000910978297 2 10 6.273698948107029 2 11 5.518304850014889 2 12 0.5626128122007863 2 13 2.4011364435451874 2 14 6.035775580246479 2 15 6.973385420998305 2 16 4.714266200550217 2 17 7.416625257926519 2 18 4.068642673788158 2 19 6.5325129305563046 2 20 5.230675263380316 2 21 7.070287563309794 2 22 7.966903110574986 2 23 6.707702368459614 2 24 8.186621191521493 2 25 6.443095617781303 2 26 5.946337144134144 2 27 5.9324827275465815 2 28 6.39568841904833 2 29 6.597419919557755 2 30 6.86260712776614 2 31 5.925525407489875 2 32 5.758514922736339 2 33 5.515899366615457 2 34 5.150180954113441 2 35 6.037081962494195 2 36 6.7012199128546115 2 37 6.708859776434725 2 38 7.139957994977575 2 39 6.613298246644444 2 40 4.191844723648693 2 41 9.137017486541147 2 42 8.090687284552065 2 43 7.613428330913134 2 44 -3.816970086698486 2 45 7.683166265499274 2 46 6.3435528160678025 2 47 3.114751740729801 2 48 6.624227832646149 2 49 -0.4714873226354115 2 50 5.959427529471334 2 51 8.297563781176493 2 52 8.680275237440014 2 53 8.648775667295421 2 54 8.986371531263696 2 55 8.587572472131313 2 56 9.674911988119568 3 1 4.472616284857239 3 2 6.279355406843088 3 3 6.176128560694117 3 4 6.205250868244043 3 5 7.08977097339265 3 6 6.557477408759201 3 7 6.168814061357807 3 8 5.949928659094091 3 9 5.865379062238802 3 10 6.668026679199468 3 11 6.999187202956062 3 12 6.782150627326839 3 13 7.995254090604011 3 14 7.3160276810868154 3 15 2.5513265974688935 3 16 7.329812419037612 3 17 8.252969795741649 3 18 9.349525026729332 3 19 8.217176796102517 3 20 7.560927364220657 3 21 7.926800168351807 3 22 9.257659413095997 3 23 8.366315813837765 3 24 8.79166320832822 3 25 7.183221668201476 3 26 7.373648068503886 3 27 7.1421465930928 3 28 7.161864889636565 3 29 7.360235045608174 3 30 7.712817211799752 3 31 6.140021205809202 3 32 6.098851057190124 3 33 6.385017013674286 3 34 5.933164147390752 3 35 6.383510533922763 3 36 7.618026635543686 3 37 8.151506835258257 3 38 8.565798331112031 3 39 9.082836061770497 3 40 11.033651446337768 3 41 13.435546898305851 3 42 12.037746108081478 3 43 12.198932532593805 3 44 12.424626551956973 3 45 11.821611643650535 3 46 13.137880547160243 3 47 13.829037455015516 3 48 13.447967328444467 3 49 14.430403433667106 3 50 13.472452739555365 3 51 13.313320083697505 3 52 12.500863472734132 3 53 12.980288272337436 3 54 13.26263203546538 3 55 13.713202724232154 3 56 12.286256525934697 4 1 4.321188322367895 4 2 6.272215594396371 4 3 6.499231714999318 4 4 6.281124696036031 4 5 6.761233036478753 4 6 6.884097187105322 4 7 5.962398886592247 4 8 5.949395837269705 4 9 5.878593043483477 4 10 6.381048844587358 4 11 7.309609197840974 4 12 7.692529996465797 4 13 8.19389006673359 4 14 7.728218644428672 4 15 8.67121150795483 4 16 8.285694771978786 4 17 9.111345754820931 4 18 9.85559919552729 4 19 8.543796574448638 4 20 7.97674151596831 4 21 7.803931455649337 4 22 8.668145346599832 4 23 7.9489031966169605 4 24 8.104323054875533 4 25 7.735331642626029 4 26 7.095301947446767 4 27 6.814780864192546 4 28 6.490615955280216 4 29 6.708807083118831 4 30 7.219957024246469 4 31 6.585566815357371 4 32 6.59650664116284 4 33 6.552855888354593 4 34 6.271506005873274 4 35 6.385322128125662 4 36 6.725443515339087 4 37 7.401613399623134 4 38 7.922256131623538 4 39 7.826548764242784 4 40 2.559546587002732 4 41 11.76962618219332 4 42 10.745759748320182 4 43 10.695415908552885 4 44 11.75135289466797 4 45 10.40718282864627 4 46 12.600902712548134 4 47 13.818167889798126 4 48 12.689548743619238 4 49 12.733792180470179 4 50 12.08541096632434 4 51 13.515472683868005 4 52 13.052014367874797 4 53 13.040390574127716 4 54 13.307282504348585 4 55 13.432085929688249 4 56 12.559487557477745 5 1 4.253410181363215 5 2 6.016031656290238 5 3 6.0567123731664765 5 4 6.260093858071549 5 5 6.969525506784951 5 6 6.2822120394535865 5 7 5.937445198022999 5 8 5.894366960730537 5 9 5.668121211730181 5 10 6.208661354122068 5 11 7.459658233473537 5 12 7.583289503930107 5 13 7.937978548808342 5 14 7.2947981366374535 5 15 9.0263929397953 5 16 8.315769959948714 5 17 9.4959485661972 5 18 9.900221621156575 5 19 8.746422704992394 5 20 7.7934572182675375 5 21 8.53156575879851 5 22 8.359384313582506 5 23 7.365597105301717 5 24 8.286245251671868 5 25 7.519411407254992 5 26 6.321901053908579 5 27 6.621580471907489 5 28 6.398319997995703 5 29 6.26508909249009 5 30 7.273133443555615 5 31 5.405224655610173 5 32 6.151372065593485 5 33 6.1914632763898965 5 34 6.402812533060807 5 35 5.880690985050581 5 36 7.089941717365889 5 37 7.596829701246758 5 38 7.928303859639232 5 39 8.711042607546698 5 40 10.500867280039675 5 41 12.76897237375919 5 42 11.789183525153568 5 43 11.967563669258997 5 44 12.840924650143617 5 45 11.722264009860176 5 46 12.928515222082844 5 47 12.93618031215837 5 48 12.21858873490179 5 49 12.807835985635268 5 50 11.638750837742009 5 51 11.423503630800553 5 52 11.496440650973852 5 53 11.55591852443828 5 54 11.411183561338888 5 55 11.583061193935823 5 56 10.642957100901523 6 1 4.34298451088781 6 2 6.050487875578611 6 3 6.352135971532469 6 4 6.290938686678501 6 5 7.15293679090075 6 6 6.606053138263601 6 7 5.8277418056838854 6 8 5.806864740613314 6 9 5.922813059459742 6 10 6.035847429546845 6 11 6.784446582298354 6 12 6.897539696340271 6 13 7.618459278190851 6 14 7.217834072934892 6 15 8.051731877691218 6 16 8.191206583002408 6 17 8.953351917094125 6 18 9.725335587173987 6 19 8.273626541354954 6 20 7.590686511974244 6 21 7.874055842274924 6 22 7.813472289427626 6 23 7.316813729539476 6 24 8.006924903557493 6 25 7.144951635779059 6 26 6.998474376980038 6 27 6.561785314723185 6 28 5.881121489914474 6 29 6.187296185024575 6 30 7.394440648576037 6 31 5.650384800065805 6 32 5.633936745008555 6 33 5.706056846930423 6 34 5.416547658275416 6 35 5.87465226620623 6 36 6.33687795559927 6 37 7.125395209505594 6 38 7.318866881366777 6 39 8.009248752831173 6 40 5.4542744199366595 6 41 11.04213559677737 6 42 9.839506182189858 6 43 10.456285473770276 6 44 11.18134262421265 6 45 10.706338368986337 6 46 11.739953266011561 6 47 12.598722494385598 6 48 11.577714896763137 6 49 12.482337199299348 6 50 12.034286922047265 6 51 11.596495164753549 6 52 11.652322481534505 6 53 11.418685678544012 6 54 11.22540415218608 6 55 10.965750199915341 6 56 9.383292190594261 7 1 4.35601964620178 7 2 6.101834398439728 7 3 6.260162174139495 7 4 6.222057590933119 7 5 6.983678536417859 7 6 6.262309341532312 7 7 5.728688010937638 7 8 5.850138743127701 7 9 6.025042927785023 7 10 6.122858611972571 7 11 6.699817455056905 7 12 7.186562392032716 7 13 8.33559022505779 7 14 7.4084646558767275 7 15 8.546074496628556 7 16 8.126030906432565 7 17 8.850658871371898 7 18 9.21822250572093 7 19 8.451420493581512 7 20 7.831935767581999 7 21 7.709363193561895 7 22 8.106398908894317 7 23 7.014425057535242 7 24 7.839982536059306 7 25 6.757801500134249 7 26 6.6425556315290955 7 27 6.100129786370115 7 28 6.345688419048333 7 29 6.29826025569221 7 30 5.995380237009833 7 31 5.727206079758783 7 32 4.974901477358188 7 33 4.81463886241378 7 34 5.14850028184453 7 35 5.21019120619167 7 36 6.651640080921837 7 37 6.913061457106995 7 38 7.178193289095223 7 39 8.310777238241084 7 40 7.732180858102478 7 41 11.071051099986526 7 42 10.625981402199123 7 43 11.103344297299689 7 44 10.951937476326723 7 45 10.898292315919441 7 46 12.204057017748475 7 47 12.978197118881063 7 48 11.759942118360438 7 49 13.162546290809967 7 50 12.237158621908307 7 51 11.07277386521011 7 52 11.86220801054926 7 53 12.177767263934076 7 54 11.738472371599833 7 55 12.08505146372795 7 56 11.508945601564946 8 1 3.7598592308161116 8 2 5.514350624442322 8 3 5.706018565476519 8 4 5.733227912720366 8 5 6.591385033650898 8 6 6.484486017080581 8 7 5.222914970013953 8 8 5.478204603618238 8 9 5.358444581129284 8 10 5.82997072511051 8 11 6.698792654892937 8 12 7.06174173206141 8 13 7.778372215902909 8 14 6.614927728910821 8 15 8.27053989254302 8 16 7.4808167231982985 8 17 8.212618289285409 8 18 8.658886576227182 8 19 7.4612585751556075 8 20 6.634217655947101 8 21 6.993574439035694 8 22 7.495822971202163 8 23 6.983544412594341 8 24 7.418038148548209 8 25 6.839785513251691 8 26 6.171830756213122 8 27 5.35065926645483 8 28 5.2725176873410184 8 29 6.797884495632086 8 30 6.790830124281818 8 31 4.772912167071755 8 32 5.453346513909395 8 33 5.323622943851234 8 34 4.811272707888119 8 35 5.162982078638212 8 36 6.354994593458564 8 37 6.173087418711152 8 38 7.332118273723221 8 39 7.33803692260263 8 40 5.214725095309554 8 41 11.18213943776066 8 42 10.268736065039867 8 43 10.212383034745887 8 44 11.00126452421905 8 45 9.933340481527154 8 46 12.41161321095747 8 47 13.046517129812266 8 48 12.73903619501549 8 49 13.339836719176436 8 50 11.856001280923131 8 51 11.444776324730498 8 52 11.046535400041641 8 53 11.086696689362785 8 54 10.481725770520374 8 55 10.864924388507617 8 56 10.163587946307722 9 1 4.240622306163402 9 2 5.483558741720344 9 3 5.871821010902551 9 4 6.031096166993464 9 5 6.99545647594012 9 6 5.6139271540116376 9 7 5.441915034030345 9 8 5.365919858723544 9 9 5.662970539774697 9 10 5.799626957105904 9 11 6.949800913007014 9 12 7.330610562481997 9 13 8.311372664017629 9 14 6.918621474509692 9 15 8.350944476116418 9 16 7.427427055443356 9 17 8.559651129692554 9 18 9.300138736780283 9 19 7.257878509858887 9 20 6.811158952919126 9 21 7.189016472196183 9 22 8.24654315556936 9 23 6.867994831901683 9 24 7.345507580722842 9 25 6.2315658090073995 9 26 6.431545243121764 9 27 6.412179015510585 9 28 6.077971883615259 9 29 6.281784374000953 9 30 6.110976081646903 9 31 5.70550291030202 9 32 5.42921233555974 9 33 5.643064720946171 9 34 4.786626398432897 9 35 5.677801872505437 9 36 6.573098428040211 9 37 6.629332217379609 9 38 7.011780267193547 9 39 7.316335367004395 9 40 4.477896467180752 9 41 9.52245055740729 9 42 10.288325079827658 9 43 9.495655552510431 9 44 11.307326876181154 9 45 9.816574589458781 9 46 11.487984762074554 9 47 12.614470525881657 9 48 11.152518046369433 9 49 12.159502553630052 9 50 11.333499520472458 9 51 10.265786503336223 9 52 11.053897284684112 9 53 11.017110875394408 9 54 10.626978955335687 9 55 10.485435239285419 9 56 9.643134710279298 10 1 4.357420206425871 10 2 6.1283750146862275 10 3 6.120246207752942 10 4 6.257211652557771 10 5 6.795653326333824 10 6 6.560908781308225 10 7 5.567833669200944 10 8 5.738163953211739 10 9 5.768810434787824 10 10 5.849889424297505 10 11 7.176638183348221 10 12 7.8149937645817396 10 13 7.884469776878515 10 14 7.290537485008379 10 15 8.948385420998306 10 16 8.14402810531212 10 17 9.264244305545567 10 18 9.335309340454828 10 19 8.603941501984874 10 20 8.018770501475554 10 21 7.64052565854789 10 22 8.339522158194033 10 23 7.639845225602471 10 24 7.423525953426253 10 25 5.970476570162262 10 26 6.117765715562715 10 27 6.228911298975152 10 28 5.8123550857150015 10 29 6.278372300510139 10 30 6.322130937289948 10 31 5.649334931299396 10 32 5.603753017974434 10 33 5.949232699948794 10 34 6.120419049351536 10 35 5.825177200589433 10 36 6.646458008092708 10 37 6.567193109768059 10 38 6.7613865664061485 10 39 7.640679199025396 10 40 10.287082818886791 10 41 10.828684153207817 10 42 9.9990206178854 10 43 10.789618807103611 10 44 10.910410865682469 10 45 10.026023408356416 10 46 10.711409958924946 10 47 11.612370788348848 10 48 11.025418308836624 10 49 11.605893629745541 10 50 10.191570386614192 10 51 11.153516162128874 10 52 11.171941904106681 10 53 10.702347095866848 10 54 10.418514388406553 10 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18 4.291898487741648 115 19 2.8934763857057995 115 20 2.740475927832147 115 21 6.6930450383928495 115 22 7.627700452767673 115 23 6.483449876765256 115 24 6.365192620092923 115 25 6.395088973263036 115 26 5.167765715562712 115 27 5.088795019905387 115 28 4.9968512097460085 115 29 5.248914936169054 115 30 5.868254968297698 115 31 4.62685431114436 115 32 4.623132862935677 115 33 4.5356668084759235 115 34 4.7554965687313775 115 35 4.67130123159718 115 36 5.332116922821392 115 37 5.8856039624812375 115 38 6.161386566406147 115 39 6.480407881195941 115 40 9.448323128964308 115 41 8.40562213770394 115 42 7.227896586877648 115 43 5.677215706328415 115 44 -0.5545116149376881 115 45 7.074860617658743 115 46 0.5631541449714597 115 47 -7.479102079868204 115 48 0.7449919522474779 115 49 -4.182284664828103 115 50 1.103779688939774 115 51 1.5409192629040689 115 52 2.204693842091178 115 53 1.1622308167970807 115 54 1.4597934581739942 115 55 2.362655528609718 115 56 4.499662818684353 116 1 4.149086873092536 116 2 5.623246809558019 116 3 5.826015438522177 116 4 5.575160370506488 116 5 6.51680717248767 116 6 6.517319037718476 116 7 5.7434746948419715 116 8 5.509317799365583 116 9 5.2386822296596165 116 10 5.95309455250263 116 11 4.572151003861041 116 12 3.598327097915071 116 13 5.768444135852878 116 14 5.584127228598128 116 15 -2.6862299636170768 116 16 4.718387079671093 116 17 6.653987895289156 116 18 6.028258058403544 116 19 5.715479963523332 116 20 5.1367192194242755 116 21 5.94885899188122 116 22 7.110034978706853 116 23 6.064845225602468 116 24 6.720961850862157 116 25 6.394194518880205 116 26 6.071611869408866 116 27 5.534680529744389 116 28 4.912996111356026 116 29 5.921321018458858 116 30 4.888156578315588 116 31 4.661514418478884 116 32 4.667855582077003 116 33 5.17743782815392 116 34 4.7883677673002545 116 35 4.65274130315353 116 36 5.760560572195274 116 37 6.197321314896268 116 38 7.142155797175381 116 39 7.3605509938971885 116 40 8.810800767604736 116 41 7.035094409618072 116 42 5.381071899936682 116 43 0.9082085506933524 116 44 2.0841288144004153 116 45 4.264484946817959 116 46 -0.9328208103058238 116 47 -6.536347160369099 116 48 0.0690080524263692 116 49 -4.097311498459586 116 50 -0.43535269030888557 116 51 3.0406956493083612 116 52 3.5206598528246325 116 53 3.2042701727899257 116 54 3.343514388406554 116 55 3.2007592853181244 116 56 5.168043856251437 117 1 2.8802512566541765 117 2 4.259311087745587 117 3 4.744218810492669 117 4 4.454243616028087 117 5 5.585036887977662 117 6 5.190132525600458 117 7 3.8919204271918133 117 8 4.0159036792391305 117 9 3.893582124285537 117 10 4.065186227950473 117 11 -0.109092410259084 117 12 -7.812631806194517 117 13 -10.33311013179728 117 14 -2.688001327777009 117 15 -5.793052935166077 117 16 -9.303346323911626 117 17 1.3165274105684013 117 18 -2.9231381481296523 117 19 0.3429825978752845 117 20 -0.3232386309445374 117 21 5.626941183662044 117 22 5.985298413901798 117 23 4.585393170807947 117 24 5.878891250229909 117 25 5.44319346513943 117 26 5.1472177703572335 117 27 5.005966093495704 117 28 4.688154172472988 117 29 5.322664537953063 117 30 5.013683448705471 117 31 3.999106620797111 117 32 3.6014699129516075 117 33 4.459050051546967 117 34 4.162314026520487 117 35 4.707140670909066 117 36 5.468147505809604 117 37 6.354065255886784 117 38 5.202482456817105 117 39 6.885313902221743 117 40 9.251923001535193 117 41 6.658250363253476 117 42 4.897536599620558 117 43 -3.1190569919831503 117 44 -3.6202968968746134 117 45 4.691776833013954 117 46 -1.187562643814779 117 47 -3.2360310381351702 117 48 -1.3418191340857497 117 49 -2.649471667058112 117 50 -1.0279501613310131 117 51 4.460479632448511 117 52 4.783699894974262 117 53 4.442415589017536 117 54 4.891459593886005 117 55 4.473678147278083 117 56 7.1391977024052835 118 1 3.37543822444389 118 2 5.0029245642357765 118 3 5.429705667212403 118 4 5.264869310215424 118 5 5.988220893901389 118 6 5.959106979506419 118 7 5.047788624155899 118 8 4.967218007265792 118 9 4.817684308661697 118 10 4.812502036910114 118 11 -0.0546681229580841 118 12 -6.6854566858687114 118 13 -5.352917610508868 118 14 -5.769822875351981 118 15 -9.703641606028722 118 16 -9.917007930723916 118 17 -0.19476470346344144 118 18 -5.771897866752383 118 19 -1.6514639034205345 118 20 -0.9100583273532727 118 21 5.529940072962301 118 22 6.310918554590426 118 23 5.645926306683549 118 24 6.527354782255085 118 25 5.940972065657753 118 26 6.316414364211358 118 27 6.013370758434615 118 28 5.48082355418347 118 29 5.438732660870496 118 30 6.218977784136797 118 31 5.409695291659755 118 32 5.2478971621185835 118 33 5.236169636885734 118 34 5.481455085387569 118 35 5.940942966355198 118 36 6.800061611696314 118 37 6.841292208867163 118 38 7.539764944784526 118 39 7.493607126953325 118 40 10.306902638706607 118 41 7.91944991897358 118 42 6.8096062034709846 118 43 1.9562854737702757 118 44 0.11739284766444769 118 45 6.88818557051858 118 46 -0.14331977080478353 118 47 0.0790374550155164 118 48 -0.3964285380102197 118 49 -1.3519892531373436 118 50 2.388192008235812 118 51 5.991579225191938 118 52 6.030275237440016 118 53 5.51923898775874 118 54 5.816487361379526 118 55 6.208035792594632 118 56 8.747305810513392 119 1 3.232947632586203 119 2 4.7297463227031 119 3 5.229739878639016 119 4 5.149405745384769 119 5 5.8510330731692655 119 6 5.372723127299782 119 7 4.932496116458331 119 8 4.438480408907935 119 9 4.770814654197103 119 10 5.26529026817936 119 11 1.399317508242735 119 12 -6.1105336615786 119 13 -2.672281277973802 119 14 -6.856719898957863 119 15 -10.03737407267258 119 16 -11.613039405236403 119 17 -0.8228864961421962 119 18 -5.70604087051564 119 19 -0.7017547005467719 119 20 -0.8871366715202242 119 21 4.967846333653373 119 22 5.739416672961969 119 23 4.761047757248036 119 24 4.320888822624571 119 25 4.544632688305718 119 26 6.680423943410812 119 27 6.4526454761903445 119 28 6.6621441152508645 119 29 6.48132588700803 119 30 6.838375663028344 119 31 6.15482016336691 119 32 5.6143015411811845 119 33 5.636785442564829 119 34 5.474954914330436 119 35 5.856611799745547 119 36 7.169031847755157 119 37 6.788606611877764 119 38 7.477842262608679 119 39 8.08888595008025 119 40 10.421681975004933 119 41 7.252840271351275 119 42 6.466636651640673 119 43 -1.8348537667360532 119 44 -0.4110026364272379 119 45 6.972858851394392 119 46 -0.3439697879104955 119 47 -2.237418241187015 119 48 -2.3829150244967074 119 49 -1.331975568566694 119 50 1.5134058296521644 119 51 6.521343166348284 119 52 6.663186629845079 119 53 6.760258488271912 119 54 5.773894135241996 119 55 7.60095402728502 119 56 9.063248335316676 120 1 3.4740868730925385 120 2 4.786708348019559 120 3 5.132746207752945 120 4 5.053044985891098 120 5 5.7456533263338265 120 6 5.894242114641553 120 7 4.926167002534278 120 8 5.225663953211736 120 9 4.864643768121155 120 10 5.154056090964172 120 11 0.7933048500148878 120 12 -3.4141729020849283 120 13 -6.373863556454815 120 14 -5.480295848324953 120 15 1.6358854209983065 120 16 -10.001805228021215 120 17 0.2059109722122372 120 18 -3.631357326211841 120 19 -0.2960584980151282 120 20 -0.43539616519111135 120 21 3.8863589918812202 120 22 6.006188824860697 120 23 5.089845225602467 120 24 5.652692620092925 120 25 5.253809903495593 120 26 5.855265715562712 120 27 5.753911298975158 120 28 5.483188419048336 120 29 6.049205633843474 120 30 5.967964270623284 120 31 5.132668264632731 120 32 4.762086351307772 120 33 5.515899366615457 120 34 5.153752382684868 120 35 5.446010533922763 120 36 5.804791341426039 120 37 4.933859776434726 120 38 5.98638656640615 120 39 6.299012532358727 120 40 7.282916152220121 120 41 -8.33798251345885 120 42 -5.3468127154479355 120 43 -7.856214526229724 120 44 -14.556255800984202 120 45 -5.29897659164358 120 46 -9.413590041075054 120 47 -7.608462544984484 120 48 -5.807915024496708 120 49 -13.548273036921126 120 50 -4.8834296133858075 120 51 -0.013150504537790653 120 52 0.05527523744001428 120 53 0.752347095866849 120 54 0.14351438840655462 120 55 1.0911439007027397 120 56 3.676697702405285 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 4419.0 5 158.0 6 309.0 7 278.0 8 358.0 9 234.0 10 190.0 11 186.0 12 196.0 13 244.0 14 281.0 15 379.0 16 462.0 17 578.0 18 672.0 19 894.0 20 1049.0 21 1308.0 22 1558.0 23 1729.0 24 2004.0 25 2296.0 26 2520.0 27 2529.0 28 2574.0 29 2946.0 30 3182.0 31 3812.0 32 4399.0 33 4857.0 34 5457.0 35 5870.0 36 6261.0 37 6957.0 38 6854.0 39 6544.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 0.0 0.0 100.0 3 0.0 0.0 0.0 100.0 4 0.0 0.0 100.0 0.0 5 100.0 0.0 0.0 0.0 6 0.0 100.0 0.0 0.0 7 95.9288916224112 3.988314192106166 0.049676511289578576 0.03311767419305238 8 99.63215726164145 0.256661974996156 0.06741812246442806 0.043762640897962085 9 98.70841070647096 0.430529764509681 0.6197736170414089 0.24128591197795307 10 43.824145150034894 34.11711828923557 14.557583356003171 7.501153204726366 11 39.13562870356133 21.244987994843108 18.233645191431986 21.385738110163576 12 28.391308976072484 28.47765148379008 18.34719150295102 24.783848037186416 13 22.004328953126663 12.163648621476812 29.749133617987628 36.0828888074089 14 21.008433179178446 26.235111831289103 31.732645747335802 21.02380924219665 15 40.47203316347742 17.119813752390456 20.532373587998432 21.8757794961337 16 37.50251452508017 24.29327053923251 20.371794720088985 17.832420215598336 17 33.944964982017794 16.412313079689568 30.712899867499527 18.929822070793108 18 22.423030976853113 17.445917655268666 23.532473062320367 36.59857830555786 19 32.55040704278682 20.739066641423705 18.75354979174555 27.956976524043924 20 31.07879247249429 22.64410151947606 27.018960882077764 19.25814512595189 21 22.040072859744992 28.010787027180466 23.033614836893527 26.91552527618101 22 18.99180337563722 17.850426390055237 39.84292760239867 23.31484263190888 23 21.105420653600955 19.27921747666978 36.3773995529114 23.237962316817864 24 28.6812627893498 19.692937321836226 22.90905219828963 28.716747690524347 25 23.963853141559426 29.142221801665407 25.934424678274038 20.959500378501136 26 30.907046920300573 27.955742263771377 19.35270102360807 21.78450979231998 27 19.25175462475293 26.993407581872624 19.39023091216816 34.364606881206285 28 24.426650265532782 34.18098808946503 20.171029131725547 21.22133251327664 29 30.417012202345695 31.560241677526356 17.96232673853809 20.06041938158986 30 28.02565862664805 26.843326153336335 26.579402087722205 18.55161313229342 31 29.3041852982232 32.90865213996735 20.66624080251733 17.120921759292116 32 31.99654564597603 30.064709988051717 19.62948504099088 18.309259324981365 33 29.527260669792998 23.613282175169342 20.39813367438776 26.461323480649902 34 22.643991467172317 22.347712728134628 37.025361460061625 17.98293434463143 35 20.556917646780136 23.589051155408686 31.972426535905907 23.88160466190527 36 25.664690639309928 31.210582987232733 25.16417592559724 17.960550447860093 37 27.119708132951125 21.933856103859185 32.600506976854376 18.345928786335318 38 28.546708701732758 23.7371702183137 23.073458648398795 24.642662431554744 39 27.428618798924692 21.674305136130553 25.79078883480774 25.10628723013702 40 21.58871371669807 17.889426860972645 24.337539055978247 36.18432036635104 41 18.718417374475862 19.694417565614213 21.43138730602444 40.15577775388549 42 27.226366536605035 26.36890854967445 21.04982269713825 25.354902216582264 43 17.8206435397055 27.150072321153345 27.02321390463093 28.006070234510233 44 21.014699247227565 29.077913134335446 23.315760727600864 26.591626890836125 45 31.237257598027597 21.215210279266035 26.20193447442037 21.345597648286 46 19.95990938096763 33.15067193300834 22.24792133698656 24.64149734903747 47 21.95950762796445 23.41737560001901 22.963499833658098 31.659616938358443 48 28.520876727892446 22.878006059458436 24.127769361863283 24.47334785078584 49 19.686570172007066 29.075239754851406 22.520952618039995 28.717237455101536 50 21.51312281533266 24.5535872978257 28.957876651460396 24.975413235381243 51 19.50397531743206 24.384715794470154 27.428503619318857 28.682805268778928 52 19.62466233827781 27.599402871901805 31.37529027060329 21.4006445192171 53 24.882606839633826 25.658113171749754 27.263672152919415 22.195607835697007 54 20.460148878668626 22.191930207197384 33.430136076999666 23.917784837134324 55 23.562414005788302 23.40347298002562 28.451629738577594 24.58248327560848 56 20.99753402570304 25.55128752311851 28.77957983591786 24.67159861526059 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 1.0 5 2.0 6 2.5 7 3.0 8 2.0 9 1.0 10 12.0 11 23.0 12 31.5 13 56.5 14 73.0 15 88.0 16 103.0 17 101.5 18 100.0 19 117.0 20 134.0 21 138.5 22 166.5 23 190.0 24 201.5 25 213.0 26 256.0 27 299.0 28 342.5 29 445.5 30 505.0 31 569.0 32 633.0 33 705.5 34 778.0 35 922.5 36 1067.0 37 1145.0 38 1326.0 39 1429.0 40 1578.0 41 1727.0 42 2138.0 43 2549.0 44 3700.5 45 4852.0 46 6494.0 47 7986.5 48 7837.0 49 6738.0 50 5639.0 51 5267.5 52 4896.0 53 4927.5 54 5291.5 55 5624.0 56 5213.5 57 4803.0 58 4953.0 59 5103.0 60 5191.5 61 5280.0 62 4930.5 63 3870.5 64 3160.0 65 2832.0 66 2504.0 67 2021.5 68 1539.0 69 1379.0 70 1219.0 71 1029.0 72 769.0 73 699.0 74 518.5 75 338.0 76 271.5 77 205.0 78 190.5 79 289.0 80 402.0 81 258.0 82 114.0 83 67.0 84 20.0 85 10.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.4234331200397412 16 0.04494541497628538 17 0.02247270748814269 18 0.0 19 0.041397092741315485 20 0.13010514861556294 21 0.0011827740783232994 22 0.0 23 0.0 24 0.0047310963132931975 25 0.003548322234969898 26 0.049676511289578576 27 0.06623534838610476 28 0.0 29 0.16204004873029204 30 0.06268702615113486 31 0.015376063018202893 32 0.01892438525317279 33 0.12182573006729984 34 0.19752327107999101 35 0.13956734124214934 36 0.04021431866299219 37 0.14784675979041242 38 0.2046199155499308 39 0.12537405230226972 40 0.8196624362780465 41 0.991164677634925 42 0.26967248985771225 43 0.2377375897429832 44 0.3844015754550723 45 0.21644765633316382 46 0.28150023064094526 47 0.45300247199782373 48 0.06032147799448827 49 0.22472707488142687 50 0.17978165990514153 51 0.32762841969555395 52 0.16913669320023184 53 0.25429642683950937 54 0.21644765633316382 55 0.28268300471926855 56 0.2353720415863366 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 84547.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.00241285911977 #Duplication Level Percentage of deduplicated Percentage of total 1 88.74839113646641 60.35104734644635 2 6.56068459317494 8.922847646870972 3 1.8419313319650747 3.7576732468331224 4 0.8435662851775837 2.2945817119472007 5 0.5061397711065502 1.7209362839604008 6 0.26089678923018056 1.0644966704909695 7 0.22263192680975408 1.0597655741776761 8 0.17219188089191917 0.9367570700320531 9 0.13044839461509028 0.798372502868227 >10 0.6087591748704213 7.512980945509598 >50 0.05913660555884093 2.7262942505352052 >100 0.03826486242042648 5.335493867316403 >500 0.006957247712804815 3.5187528830118158 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ACCTGAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT 890 1.0526689297077365 No Hit ACCTGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 755 0.892994429134091 No Hit ACCTGAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC 715 0.8456834660011591 No Hit ACCTGAGGGAGATTGGGCGGATTGAACAAAAACTCATCTCAGAAGAGGATCTGTGA 615 0.7274060581688292 No Hit ACCTGAAGGAGATTGGGCGGATTGAACAAAAACTCATCTCAGAAGAGGATCTGTGA 467 0.5523554945769809 No Hit ACCTGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 370 0.4376264089796208 No Hit ACCTGAGGGAGATTGGGCGGATTGAACAAAAACTCATCTCCGAAGAGGATCTGTGA 345 0.4080570570215383 No Hit ACCTGAGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT 337 0.3985948643949519 No Hit ACCTGAGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC 275 0.32526287153890737 No Hit ACCTGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 274 0.3240800974605841 No Hit ACCTGAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC 249 0.29451074550250156 No Hit ACCTGAGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT 235 0.2779519084059754 No Hit ACCTGAAGGAGATTGGGCGGATTGAACAAAAACTCATCTCCGAAGAGGATCTGTGA 224 0.26494139354441903 No Hit ACCTGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 194 0.2294581711947201 No Hit ACCTGAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT 178 0.21053378594154729 No Hit ACCTGAGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC 176 0.20816823778490068 No Hit ACCTGAGGGGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCAT 146 0.17268501543520173 No Hit ACCTGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC 144 0.17031946727855513 No Hit ACCTGAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA 126 0.14902953386873571 No Hit ACCTGAGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA 124 0.14666398571208913 No Hit ACCTGAGGGATCAGATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGG 123 0.14548121163376582 No Hit ACCTGAGGGAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTG 110 0.13010514861556294 No Hit ACCTGAGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATG 110 0.13010514861556294 No Hit ACCTGAGGGGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGC 102 0.12064295598897654 No Hit ACCTGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG 101 0.11946018191065323 No Hit ACCTGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG 101 0.11946018191065323 No Hit ACCTGAGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC 99 0.11709463375400664 No Hit ACCTGAGGGAACTGTGGTAATTCTAGAGCTAATACATGCCCACGGGCGCTGACCCC 91 0.10763244112742025 No Hit ACCTGAGGGACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTT 89 0.10526689297077364 No Hit ACCTGAGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGG 89 0.10526689297077364 No Hit ACCTGAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCCGATTGAACAAAAACTC 86 0.10171857073580375 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0011827740783232994 0.0 0.0 0.0 0.0 7 0.0011827740783232994 0.0 0.0 0.0 0.0 8 0.0011827740783232994 0.0 0.0 0.0 0.0 9 0.0011827740783232994 0.0 0.0 0.0 0.0 10 0.026021029723112588 0.0 0.0 0.0 0.0 11 0.029569351958082486 0.0 0.0 0.0 0.0 12 0.03193490011472908 0.0 0.0 0.0 0.0 13 0.033117674193052386 0.0 0.0 0.0 0.0 14 0.05440760760287178 0.0 0.0 0.0 0.0 15 0.05440760760287178 0.0 0.0 0.0 0.0 16 0.057955929837841674 0.0 0.0 0.0 0.0 17 0.05913870391616497 0.0 0.0 0.0 0.0 18 0.060321477994488275 0.0 0.0 0.0 0.0 19 0.06386980022945817 0.0 0.0 0.0 0.0 20 0.06623534838610477 0.0 0.0 0.0 0.0 21 0.07096644469939797 0.0 0.0 0.0 0.0 22 0.07096644469939797 0.0 0.0 0.0 0.0 23 0.07451476693436787 0.0 0.0 0.0 0.0 24 0.07924586324766106 0.0 0.0 0.0 0.0 25 0.09698747442251056 0.0 0.0 0.0 0.0 26 0.10999798928406684 0.0 0.0 0.0 0.0 27 0.12064295598897655 0.0 0.0 0.0 0.0 28 0.17150224135687842 0.0 0.0 0.0 0.0 29 0.2519308786828628 0.0 0.0 0.0 0.0 30 0.28741410103256176 0.0 0.0 0.0 0.0 31 0.303972938129088 0.0 0.0 0.0 0.0 32 0.34063893455711025 0.0 0.0 0.0 0.0 33 0.34537003087040347 0.0 0.0 0.0 0.0 34 0.34655280494872676 0.0 0.0 0.0 0.0 35 0.3512839012620199 0.0 0.0 0.0 0.0 36 0.3631116420452529 0.0 0.0 0.0 0.0 37 0.3642944161235762 0.0 0.0 0.0 0.0 38 0.36547719020189956 0.0 0.0 0.0 0.0 39 0.37257383467183935 0.0 0.0 0.0 0.0 40 0.37257383467183935 0.0 0.0 0.0 0.0 41 0.3761221569068092 0.0 0.0 0.0 0.0 42 0.38203602729842573 0.0 0.0 0.0 0.0 43 0.39386376808165874 0.0 0.0 0.0 0.0 44 0.41397092741315483 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CACCCGC 20 3.900272E-4 50.660847 41 AGCCGAC 15 0.007978635 50.660843 41 CCAATCC 25 1.915882E-5 50.660843 41 GTTTAAT 20 3.935219E-4 50.569454 39 CTACGAA 15 0.008035834 50.56945 38 TACGAAG 15 0.008035834 50.56945 39 ACTCGCC 15 0.008035834 50.56945 39 TACTCGC 15 0.008035834 50.56945 38 TCTACGA 15 0.008054968 50.53906 37 TTGTTCG 15 0.008054968 50.53906 37 GCGACCA 15 0.008131843 50.417866 36 ATCTACG 20 3.9940162E-4 50.417866 36 TATGCTC 25 1.971236E-5 50.417866 35 TTAAGCG 15 0.008131843 50.417866 36 ATGCCCA 15 0.008131843 50.417866 36 ATATGCA 15 0.008189857 50.327347 34 TAATCCG 15 0.008228703 50.267185 42 GAAGACG 35 4.996582E-8 50.26718 42 AGACGAT 40 2.5229383E-9 50.23716 44 CCCCTAC 25 2.0136156E-5 50.237156 45 >>END_MODULE