FastQCFastQC Report
Fri 17 Jun 2016
SRR1525937_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525937_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3448
Sequences flagged as poor quality0
Sequence length40
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTGAGGGCTCGTCTTACTCAGCAACATCTTTTGATACA80.23201856148491878No Hit
ACCTGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT80.23201856148491878No Hit
ACCTGAGGGCTCGTCTTAATCAGCAACATCTTTTGATACA60.17401392111368907No Hit
ACCTGAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT60.17401392111368907No Hit
ACCTGAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT60.17401392111368907No Hit
ACCTGAGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAA50.14501160092807425No Hit
ACCTGAGGGAAGTGTGTGGGTTGTTAGACTATTTCTCCCT50.14501160092807425No Hit
ACCTGAGGGGCCGAATAGCCTCTCCACCCAAGCGGCCCGA50.14501160092807425No Hit
ACCTGAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT40.11600928074245939No Hit
ACCTGAGGGGCCGAATAGCCTCTCCACCCCAGCGGCCGGA40.11600928074245939No Hit
ACCTGAGGGAGTCGACTTCTTTTTTTTTTTTTTTTTTTTT40.11600928074245939No Hit
ACCTGAGGGACTCTTTCAGGTCACTCTGGGGCTGCCTCTT40.11600928074245939No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGAAG501.7826096E-1034.2985086
GGAAGCA200.00231102734.2985088
TGAGGGT356.751561E-734.2985084
TGAGGGG1000.034.2985084
GGGAAGT200.00231102734.2985087
GGGAAGC251.5483066E-434.2985087
GAGGGGT301.0276313E-534.2985085
GAGGGGC301.0276313E-534.2985085
GAGGGGA301.0276313E-534.2985085
GAGGGCT251.5483066E-434.2985085
GAGGGCA200.00231102734.2985085
GAGGGAT301.0276313E-534.2985085
GAGGGAC251.5483066E-434.2985085
GAGGGAA551.0913936E-1134.2985085
CTGAGGG3350.033.2746733
TGAGGGA1300.032.9793324
ACCTGAG3500.032.828571
CCTGAGG3450.032.814582
TGAGGGC700.031.8486164
GAGGGCC250.006896092627.4388075
Produced by FastQC (version 0.11.5)