##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1525937_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3448
Sequences flagged as poor quality	0
Sequence length	40
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.310034802784223	28.0	21.0	31.0	4.0	33.0
2	13.277262180974478	11.0	11.0	15.0	4.0	23.0
3	18.842227378190255	19.0	11.0	28.0	4.0	33.0
4	21.666183294663572	24.0	14.0	30.0	4.0	33.0
5	21.188515081206496	23.0	14.0	29.0	4.0	33.0
6	22.780742459396752	26.0	16.0	31.0	4.0	34.0
7	21.163863109048723	24.0	11.0	30.0	4.0	33.0
8	22.233758700696054	26.0	15.0	31.0	4.0	33.0
9	21.121519721577727	24.0	12.0	30.0	4.0	33.0
10	18.411252900232018	19.0	10.0	27.0	4.0	31.0
11	23.15052204176334	27.0	13.0	33.0	4.0	36.0
12	23.183584686774942	27.0	13.0	33.0	4.0	37.0
13	23.9411252900232	28.0	14.0	34.0	4.0	37.0
14	24.369489559164734	29.0	15.0	35.0	4.0	37.0
15	22.87587006960557	27.0	11.0	34.0	4.0	37.0
16	20.339327146171694	23.0	4.0	32.0	4.0	35.0
17	20.88834106728538	25.0	4.0	32.0	4.0	35.0
18	21.100348027842227	26.0	4.0	33.0	4.0	36.0
19	17.912122969837586	18.0	4.0	30.0	4.0	34.0
20	19.66792343387471	23.0	4.0	32.0	4.0	35.0
21	17.49332946635731	17.0	4.0	30.0	4.0	35.0
22	17.38022041763341	17.0	4.0	30.0	4.0	34.0
23	17.45707656612529	17.0	4.0	30.0	4.0	35.0
24	16.910962877030162	16.0	4.0	30.0	4.0	33.0
25	16.15893271461717	12.0	4.0	29.0	4.0	33.0
26	14.273781902552205	4.0	4.0	27.0	4.0	33.0
27	14.078886310904872	4.0	4.0	27.0	4.0	33.0
28	13.85324825986079	4.0	4.0	27.0	4.0	33.0
29	13.361658932714617	4.0	4.0	26.0	4.0	33.0
30	12.831206496519721	4.0	4.0	25.0	4.0	33.0
31	12.564385150812065	4.0	4.0	25.0	4.0	33.0
32	12.212296983758701	4.0	4.0	24.0	4.0	32.0
33	11.473897911832946	4.0	4.0	20.0	4.0	31.0
34	10.794373549883991	4.0	4.0	17.0	4.0	31.0
35	10.475638051044083	4.0	4.0	16.0	4.0	31.0
36	10.452436194895592	4.0	4.0	15.0	4.0	32.0
37	10.329176334106728	4.0	4.0	15.0	4.0	32.0
38	9.805394431554525	4.0	4.0	15.0	4.0	31.0
39	9.52639211136891	4.0	4.0	13.0	4.0	31.0
40	9.39385150812065	4.0	4.0	4.0	4.0	31.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	8.633877551020404
1	2	20.0668820861678
1	3	-1.2083446712018144
1	4	11.395317460317461
1	5	10.44418367346939
1	6	10.963843537414967
1	7	11.461213151927439
1	8	7.126859410430853
1	9	2.7570975056689377
1	10	10.255045351473921
1	11	3.807018140589573
1	12	1.4645578231292475
1	13	-0.5830272108843566
1	14	3.9304988662131493
1	15	7.372437641723359
1	16	1.8635714285714222
1	17	8.75013605442177
1	18	5.823412698412703
1	19	-3.695226757369616
1	20	8.935408163265308
1	21	10.445566893424035
1	22	2.1163265306122447
1	23	3.3363605442176905
1	24	6.458117913832199
1	25	10.540442176870753
1	26	11.485419501133789
1	27	5.055374149659862
1	28	14.38039682539682
1	29	12.284659863945578
1	30	6.569909297052153
1	31	11.097131519274377
1	32	11.12104308390023
1	33	6.068253968253966
1	34	7.594342403628117
1	35	6.10484126984127
1	36	8.580283446712018
1	37	7.893401360544214
1	38	6.149331065759636
1	39	10.959659863945578
1	40	9.554965986394555
5	1	1.8481632653061197
5	2	1.4954535147392303
5	3	-6.92263038548753
5	4	-1.3189682539682543
5	5	-0.9843877551020377
5	6	5.892414965986397
5	7	-13.181643990929704
5	8	-14.087426303854862
5	9	-12.385759637188205
5	10	-2.3878117913832213
5	11	-8.764410430839
5	12	-11.749727891156468
5	13	-12.797312925170072
5	14	-12.283786848072566
5	15	-11.413276643990926
5	16	0.14928571428570692
5	17	-1.2498639455782303
5	18	-2.3194444444444393
5	19	2.3762018140589554
5	20	-6.350306122448977
5	21	0.3027097505668923
5	22	-1.16938775510204
5	23	0.33636054421769046
5	24	-3.827596371882086
5	25	1.6832993197278956
5	26	-6.086009070294784
5	27	-6.0874829931972805
5	28	4.38039682539682
5	29	4.784659863945578
5	30	5.069909297052153
5	31	-8.902868480725623
5	32	-8.021814058956913
5	33	-7.2174603174603185
5	34	-6.262800453514741
5	35	-6.752301587301588
5	36	-5.991145124716555
5	37	-5.963741496598644
5	38	-5.5649546485260775
5	39	-5.183197278911564
5	40	-4.873605442176872
6	1	2.681496598639452
6	2	4.828786848072562
6	3	1.0773696145124703
6	4	2.6810317460317457
6	5	2.68227891156463
6	6	3.059081632653065
6	7	-0.6816439909297038
6	8	-2.25409297052153
6	9	4.280907029478463
6	10	2.1121882086167787
6	11	9.902256235827668
6	12	8.750272108843532
6	13	15.036020408163264
6	14	9.54954648526077
6	15	8.086723356009074
6	16	13.149285714285707
6	17	8.083469387755102
6	18	10.68055555555556
6	19	10.876201814058955
6	20	14.649693877551023
6	21	6.302709750566892
6	22	14.83061224489796
6	23	5.003027210884358
6	24	5.505736961451246
6	25	11.683299319727896
6	26	11.580657596371884
6	27	8.579183673469387
6	28	13.047063492063488
6	29	17.951326530612246
6	30	11.23657596371882
6	31	10.763798185941045
6	32	5.311519274376419
6	33	10.782539682539682
6	34	-0.5961337868480747
6	35	-0.08563492063492184
6	36	2.6755215419501113
6	37	2.7029251700680224
6	38	-5.5649546485260775
6	39	-5.183197278911564
6	40	-4.873605442176872
7	1	11.148163265306117
7	2	11.69545351473923
7	3	12.877369614512471
7	4	13.681031746031746
7	5	14.215612244897965
7	6	14.5924149659864
7	7	9.918356009070298
7	8	6.812573696145137
7	9	3.6142403628117954
7	10	16.31218820861678
7	11	9.035589569161
7	12	4.550272108843533
7	13	9.902687074829931
7	14	5.016213151927435
7	15	2.6867233560090753
7	16	2.7492857142857083
7	17	6.950136054421769
7	18	0.6805555555555607
7	19	9.676201814058956
7	20	5.449693877551024
7	21	10.702709750566891
7	22	11.430612244897961
7	23	10.53636054421769
7	24	8.372403628117913
7	25	8.083299319727894
7	26	4.113990929705215
7	27	5.312517006802718
7	28	7.180396825396821
7	29	8.484659863945577
7	30	9.369909297052153
7	31	-2.5028684807256223
7	32	1.5781859410430865
7	33	-2.4174603174603178
7	34	-3.662800453514741
7	35	-5.552301587301588
7	36	-3.7911451247165546
7	37	-0.9637414965986437
7	38	-0.7649546485260768
7	39	-0.1831972789115639
7	40	0.7263945578231272
8	1	-0.6518367346938803
8	2	0.6954535147392296
8	3	8.477369614512469
8	4	5.481031746031746
8	5	2.2156122448979616
8	6	0.9924149659863986
8	7	-4.481643990929705
8	8	-8.387426303854863
8	9	-8.385759637188205
8	10	-3.8878117913832213
8	11	6.035589569161001
8	12	11.350272108843534
8	13	3.1026870748299267
8	14	7.6162131519274325
8	15	-3.5132766439909275
8	16	-2.2507142857142917
8	17	5.55013605442177
8	18	8.280555555555562
8	19	11.076201814058955
8	20	0.44969387755102375
8	21	8.302709750566892
8	22	8.03061224489796
8	23	11.33636054421769
8	24	6.3724036281179135
8	25	-1.3167006802721044
8	26	6.313990929705215
8	27	-1.6874829931972801
8	28	6.180396825396821
8	29	-0.9153401360544215
8	30	0.7699092970521519
8	31	1.697131519274377
8	32	2.7781859410430876
8	33	-1.817460317460318
8	34	-2.8628004535147404
8	35	-1.3523015873015876
8	36	-0.5911451247165544
8	37	-4.163741496598644
8	38	-2.3649546485260773
8	39	0.21680272108843646
8	40	0.5263945578231279
9	1	9.47316326530612
9	2	8.62045351473923
9	3	14.07736961451247
9	4	7.181031746031746
9	5	10.640612244897962
9	6	10.267414965986397
9	7	12.193356009070296
9	8	9.662573696145138
9	9	11.614240362811795
9	10	14.237188208616779
9	11	8.985589569161
9	12	7.500272108843532
9	13	7.702687074829928
9	14	3.591213151927434
9	15	7.086723356009074
9	16	9.899285714285707
9	17	9.75013605442177
9	18	11.68055555555556
9	19	9.251201814058955
9	20	13.524693877551023
9	21	13.927709750566892
9	22	5.33061224489796
9	23	16.83636054421769
9	24	13.922403628117914
9	25	15.308299319727896
9	26	3.288990929705216
9	27	2.7875170068027195
9	28	0.6303968253968204
9	29	-2.3403401360544223
9	30	1.3199092970521527
9	31	1.3471315192743774
9	32	1.6031859410430869
9	33	1.5325396825396815
9	34	4.112199546485259
9	35	3.747698412698412
9	36	4.258854875283445
9	37	6.036258503401356
9	38	1.5600453514739225
9	39	3.191802721088436
9	40	3.2513945578231276
10	1	6.633877551020404
10	2	-0.21883219954648325
10	3	-4.494058956916101
10	4	1.823888888888888
10	5	8.44418367346939
10	6	9.106700680272112
10	7	10.175498866213154
10	8	10.412573696145138
10	9	10.185668934240368
10	10	6.826473922902494
10	11	11.092732426303854
10	12	10.893129251700678
10	13	9.131258503401355
10	14	7.787641723356007
10	15	5.9438662131519315
10	16	6.149285714285707
10	17	7.035850340136054
10	18	5.966269841269845
10	19	8.733344671201813
10	20	3.07826530612245
10	21	2.3027097505668923
10	22	5.116326530612245
10	23	3.479217687074833
10	24	-0.39902494331065697
10	25	0.11187074829932442
10	26	0.4854195011337872
10	27	-1.6589115646258517
10	28	0.9518253968253916
10	29	-1.2867687074829934
10	30	2.4270521541950103
10	31	-6.902868480725623
10	32	-5.307528344671199
10	33	-4.78888888888889
10	34	-3.6913718820861696
10	35	-4.323730158730159
10	36	-3.4197165532879836
10	37	-2.106598639455787
10	38	-1.9935260770975063
10	39	-1.754625850340135
10	40	-1.0164625850340157
11	1	9.514829931972784
11	2	5.99545351473923
11	3	6.910702947845802
11	4	4.3476984126984135
11	5	4.8489455782312945
11	6	7.559081632653065
11	7	7.485022675736964
11	8	6.912573696145138
11	9	6.280907029478463
11	10	12.44552154195011
11	11	10.235589569161
11	12	9.083605442176864
11	13	7.202687074829928
11	14	12.382879818594098
11	15	8.086723356009074
11	16	4.149285714285707
11	17	11.25013605442177
11	18	7.513888888888893
11	19	13.709535147392288
11	20	13.649693877551023
11	21	11.302709750566892
11	22	9.33061224489796
11	23	11.503027210884358
11	24	12.005736961451246
11	25	13.683299319727896
11	26	7.247324263038548
11	27	3.4125170068027195
11	28	6.2137301587301526
11	29	0.9513265306122438
11	30	0.9032426303854866
11	31	-3.7362018140589566
11	32	-3.688480725623579
11	33	-3.2174603174603185
11	34	-2.596133786848074
11	35	-1.918968253968254
11	36	-3.6578117913832218
11	37	-4.963741496598644
11	38	-3.5649546485260775
11	39	-2.849863945578231
11	40	-0.5402721088435385
12	1	-5.366122448979596
12	2	-0.3616893424036274
12	3	1.2202267573696126
12	4	4.681031746031746
12	5	6.444183673469389
12	6	1.6781292517006818
12	7	-0.1102154195011309
12	8	3.841145124716565
12	9	0.7570975056689377
12	10	1.9693310657596363
12	11	10.235589569161
12	12	8.035986394557817
12	13	7.416972789115643
12	14	6.501927437641719
12	15	12.22958049886622
12	16	9.72071428571428
12	17	14.75013605442177
12	18	12.251984126984134
12	19	12.447630385487528
12	20	16.221122448979592
12	21	14.15985260770975
12	22	10.259183673469387
12	23	13.622074829931975
12	24	9.88668934240363
12	25	13.254727891156469
12	26	6.199705215419501
12	27	7.341088435374147
12	28	12.237539682539678
12	29	5.42751700680272
12	30	9.141337868480726
12	31	4.66856009070295
12	32	4.263900226757372
12	33	4.925396825396824
12	34	-0.834229024943312
12	35	3.676269841269841
12	36	-0.7054308390022683
12	37	-1.9637414965986437
12	38	-2.4220975056689342
12	39	-1.183197278911564
12	40	-3.3021768707483012
13	1	5.919591836734693
13	2	2.6383106575963726
13	3	3.2202267573696126
13	4	6.823888888888888
13	5	4.729897959183678
13	6	6.106700680272112
13	7	0.6040702947845809
13	8	0.12685941043085336
13	9	1.1856689342403683
13	10	4.540759637188206
13	11	10.092732426303858
13	12	9.321700680272105
13	13	8.559829931972786
13	14	10.787641723356003
13	15	9.229580498866216
13	16	11.149285714285707
13	17	10.892993197278912
13	18	7.966269841269845
13	19	9.876201814058955
13	20	12.50683673469388
13	21	11.588424036281177
13	22	10.973469387755102
13	23	10.479217687074833
13	24	15.172403628117914
13	25	13.683299319727896
13	26	3.1997052154195007
13	27	6.769659863945577
13	28	7.38039682539682
13	29	7.713231292517005
13	30	8.42705215419501
13	31	9.954274376417235
13	32	5.692471655328802
13	33	5.3539682539682545
13	34	6.165770975056686
13	35	6.390555555555554
13	36	6.1517120181405875
13	37	4.75054421768707
13	38	4.29218820861678
13	39	4.388231292517007
13	40	1.840680272108841
14	1	-3.6518367346938803
14	2	4.49545351473923
14	3	-0.9226303854875297
14	4	1.6810317460317457
14	5	13.015612244897962
14	6	13.392414965986397
14	7	13.318356009070296
14	8	14.412573696145138
14	9	-4.385759637188205
14	10	4.112188208616779
14	11	9.235589569161
14	12	-8.249727891156468
14	13	-9.297312925170072
14	14	12.216213151927434
14	15	-3.913276643990926
14	16	14.149285714285707
14	17	10.75013605442177
14	18	-2.3194444444444393
14	19	16.876201814058955
14	20	15.649693877551023
14	21	17.30270975056689
14	22	2.83061224489796
14	23	17.33636054421769
14	24	19.172403628117912
14	25	-10.316700680272104
14	26	-9.086009070294784
14	27	-9.08748299319728
14	28	-8.61960317460318
14	29	-8.715340136054422
14	30	-8.430090702947847
14	31	-8.902868480725623
14	32	-8.021814058956913
14	33	-7.2174603174603185
14	34	-6.262800453514741
14	35	-6.752301587301588
14	36	-5.991145124716555
14	37	-5.963741496598644
14	38	-5.5649546485260775
14	39	-5.183197278911564
14	40	-4.873605442176872
15	1	10.84816326530612
15	2	6.99545351473923
15	3	7.07736961451247
15	4	12.431031746031746
15	5	12.015612244897962
15	6	11.142414965986397
15	7	11.818356009070296
15	8	11.912573696145138
15	9	7.114240362811795
15	10	15.112188208616779
15	11	8.985589569161
15	12	3.2502721088435322
15	13	5.702687074829928
15	14	6.216213151927434
15	15	8.836723356009074
15	16	5.399285714285707
15	17	7.75013605442177
15	18	8.18055555555556
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102	25	-10.316700680272104
102	26	-9.086009070294784
102	27	-9.08748299319728
102	28	-8.61960317460318
102	29	-8.715340136054422
102	30	-8.430090702947847
102	31	-8.902868480725623
102	32	-8.021814058956913
102	33	-7.2174603174603185
102	34	-6.262800453514741
102	35	-6.752301587301588
102	36	-5.991145124716555
102	37	-5.963741496598644
102	38	-5.5649546485260775
102	39	-5.183197278911564
102	40	-4.873605442176872
103	1	0.6814965986394519
103	2	-0.1712131519274358
103	3	4.410702947845802
103	4	-7.318968253968254
103	5	-4.317721088435372
103	6	0.39241496598639714
103	7	-6.681643990929704
103	8	-9.254092970521528
103	9	1.6142403628117954
103	10	-0.8878117913832213
103	11	-6.431077097505666
103	12	-0.5830612244897999
103	13	-9.297312925170072
103	14	-3.117120181405898
103	15	-0.9132766439909261
103	16	-3.850714285714293
103	17	2.7501360544217697
103	18	3.013888888888893
103	19	-3.1237981859410446
103	20	-6.016972789115643
103	21	-9.030623582766442
103	22	-5.836054421768706
103	23	-3.6636394557823095
103	24	-9.494263038548752
103	25	-1.9833673469387705
103	26	-0.086009070294784
103	27	-0.4208163265306144
103	28	0.3803968253968204
103	29	-0.38200680272108833
103	30	-1.7634240362811813
103	31	0.09713151927437735
103	32	0.9781859410430869
103	33	1.7825396825396815
103	34	2.737199546485259
103	35	-3.752301587301588
103	36	-2.324478458049888
103	37	4.36959183673469
103	38	-1.8982879818594105
103	39	-1.516530612244897
103	40	-1.2069387755102055
104	1	2.7481632653061183
104	2	0.8954535147392306
104	3	3.0773696145124703
104	4	5.081031746031744
104	5	-0.5843877551020391
104	6	2.5924149659863964
104	7	-0.8816439909297031
104	8	-0.9874263038548605
104	9	-2.585759637188204
104	10	3.9121882086167794
104	11	5.235589569161
104	12	7.150272108843531
104	13	1.702687074829928
104	14	2.6162131519274325
104	15	-3.5132766439909275
104	16	2.349285714285706
104	17	-4.049863945578231
104	18	-3.11944444444444
104	19	4.076201814058955
104	20	-1.7503061224489773
104	21	-6.297290249433107
104	22	-9.96938775510204
104	23	-5.46363945578231
104	24	-6.427596371882085
104	25	-4.116700680272105
104	26	-9.086009070294784
104	27	-9.08748299319728
104	28	-8.61960317460318
104	29	-8.715340136054422
104	30	-8.430090702947847
104	31	-8.902868480725623
104	32	-8.021814058956913
104	33	-7.2174603174603185
104	34	-6.262800453514741
104	35	-6.752301587301588
104	36	-5.991145124716555
104	37	-5.963741496598644
104	38	-5.5649546485260775
104	39	-5.183197278911564
104	40	-4.873605442176872
105	1	7.014829931972788
105	2	-2.5045464852607697
105	3	-1.9226303854875297
105	4	-6.652301587301588
105	5	-6.317721088435372
105	6	-0.940918367346935
105	7	-6.681643990929704
105	8	-10.254092970521528
105	9	-2.3857596371882046
105	10	-1.8878117913832213
105	11	-1.4310770975056677
105	12	-3.9163945578231356
105	13	-0.2973129251700719
105	14	-8.783786848072566
105	15	-6.913276643990926
105	16	-3.184047619047627
105	17	-9.916530612244896
105	18	0.013888888888892836
105	19	-6.790464852607711
105	20	-4.016972789115643
105	21	2.3027097505668923
105	22	7.83061224489796
105	23	2.0030272108843583
105	24	3.172403628117914
105	25	2.3499659863945634
105	26	1.24732426303855
105	27	0.5791836734693856
105	28	-1.9529365079365135
105	29	1.6179931972789117
105	30	-1.0967573696145134
105	31	1.4304648526077113
105	32	2.311519274376421
105	33	-2.8841269841269845
105	34	2.737199546485259
105	35	-2.418968253968254
105	36	-1.6578117913832209
105	37	-1.6304081632653098
105	38	-1.2316213151927435
105	39	-0.84986394557823
105	40	-0.5402721088435385
106	1	-2.9018367346938803
106	2	-3.2545464852607697
106	3	-0.6726303854875297
106	4	0.18103174603174566
106	5	-2.2343877551020377
106	6	-1.3575850340136029
106	7	-8.181643990929704
106	8	-3.837426303854862
106	9	-3.6357596371882046
106	10	-4.637811791383221
106	11	-8.514410430839
106	12	-3.7497278911564678
106	13	-0.04731292517007191
106	14	-0.783786848072566
106	15	-6.413276643990926
106	16	-4.850714285714293
106	17	-4.49986394557823
106	18	-0.06944444444443931
106	19	-4.873798185941045
106	20	-6.850306122448977
106	21	-9.947290249433108
106	22	-7.41938775510204
106	23	-7.9136394557823095
106	24	-4.577596371882086
106	25	-8.566700680272104
106	26	-7.336009070294784
106	27	-3.0874829931972805
106	28	-1.8696031746031796
106	29	-2.2153401360544223
106	30	-3.6800907029478473
106	31	-2.9028684807256226
106	32	-0.7718140589569131
106	33	-7.2174603174603185
106	34	-6.262800453514741
106	35	-6.752301587301588
106	36	-5.991145124716555
106	37	-5.963741496598644
106	38	-5.5649546485260775
106	39	-5.183197278911564
106	40	-4.873605442176872
107	1	1.3481632653061197
107	2	-0.5045464852607697
107	3	4.244036281179138
107	4	2.1810317460317457
107	5	-1.1510544217687055
107	6	0.22574829931972928
107	7	0.485022675736964
107	8	-5.754092970521528
107	9	1.9475736961451275
107	10	4.612188208616779
107	11	2.402256235827668
107	12	1.5836054421768644
107	13	-0.46397959183673976
107	14	5.716213151927434
107	15	-1.9132766439909261
107	16	-0.18404761904762523
107	17	-0.9165306122448982
107	18	-5.319444444444439
107	19	-7.790464852607711
107	20	-8.516972789115643
107	21	-1.3639569160997738
107	22	-0.16938775510203996
107	23	-1.4969727891156435
107	24	-1.4942630385487519
107	25	2.0166326530612277
107	26	5.247324263038548
107	27	4.579183673469387
107	28	6.047063492063488
107	29	4.451326530612246
107	30	5.069909297052153
107	31	8.263798185941045
107	32	8.811519274376419
107	33	9.282539682539682
107	34	7.403866213151927
107	35	7.747698412698412
107	36	9.342188208616777
107	37	4.036258503401356
107	38	7.60171201814059
107	39	4.816802721088436
107	40	5.126394557823128
108	1	3.3481632653061197
108	2	0.49545351473923027
108	3	-0.9226303854875297
108	4	-3.3189682539682543
108	5	7.515612244897962
108	6	-5.107585034013603
108	7	-3.181643990929704
108	8	0.41257369614513806
108	9	-1.3857596371882046
108	10	-6.387811791383221
108	11	3.235589569161
108	12	8.750272108843532
108	13	7.202687074829928
108	14	-0.783786848072566
108	15	-0.4132766439909261
108	16	3.149285714285707
108	17	12.75013605442177
108	18	13.18055555555556
108	19	-5.623798185941045
108	20	-2.350306122448977
108	21	4.802709750566892
108	22	3.33061224489796
108	23	2.8363605442176905
108	24	4.172403628117914
108	25	6.183299319727896
108	26	5.413990929705216
108	27	6.4125170068027195
108	28	7.38039682539682
108	29	8.284659863945578
108	30	7.069909297052153
108	31	7.097131519274377
108	32	7.478185941043087
108	33	-7.2174603174603185
108	34	-6.262800453514741
108	35	-6.752301587301588
108	36	-5.991145124716555
108	37	-5.963741496598644
108	38	-5.5649546485260775
108	39	-5.183197278911564
108	40	-4.873605442176872
109	1	0.548163265306119
109	2	-2.90454648526077
109	3	-1.3226303854875283
109	4	2.0810317460317442
109	5	-0.9843877551020377
109	6	-0.007585034013601444
109	7	1.5183560090702954
109	8	1.8125736961451366
109	9	2.414240362811796
109	10	3.1121882086167787
109	11	0.43558956916099945
109	12	2.9502721088435315
109	13	3.702687074829928
109	14	5.016213151927435
109	15	1.0867233560090739
109	16	-0.6507142857142938
109	17	2.350136054421771
109	18	5.680555555555561
109	19	0.8762018140589554
109	20	6.449693877551024
109	21	-1.4972902494331084
109	22	4.4306122448979615
109	23	1.9363605442176919
109	24	1.5724036281179128
109	25	3.283299319727897
109	26	7.713990929705217
109	27	6.112517006802719
109	28	6.58039682539682
109	29	5.884659863945579
109	30	4.769909297052152
109	31	12.897131519274378
109	32	8.578185941043088
109	33	13.582539682539682
109	34	5.9371995464852585
109	35	15.64769841269841
109	36	2.2088548752834445
109	37	5.436258503401357
109	38	4.835045351473923
109	39	9.416802721088438
109	40	3.9263945578231283
110	1	4.34816326530612
110	2	-1.5045464852607697
110	3	-1.9226303854875297
110	4	5.681031746031746
110	5	-3.9843877551020377
110	6	4.892414965986397
110	7	-1.6816439909297038
110	8	8.912573696145138
110	9	3.1142403628117954
110	10	7.112188208616779
110	11	-1.2644104308389998
110	12	13.750272108843532
110	13	-7.797312925170072
110	14	-11.783786848072566
110	15	-1.4132766439909261
110	16	-14.850714285714293
110	17	-14.24986394557823
110	18	-15.31944444444444
110	19	-12.123798185941045
110	20	-13.350306122448977
110	21	-11.697290249433108
110	22	-11.16938775510204
110	23	-11.66363945578231
110	24	-11.827596371882086
110	25	-10.316700680272104
110	26	-9.086009070294784
110	27	-9.08748299319728
110	28	-8.61960317460318
110	29	-8.715340136054422
110	30	-8.430090702947847
110	31	-8.902868480725623
110	32	-8.021814058956913
110	33	-7.2174603174603185
110	34	-6.262800453514741
110	35	-6.752301587301588
110	36	-5.991145124716555
110	37	-5.963741496598644
110	38	-5.5649546485260775
110	39	-5.183197278911564
110	40	-4.873605442176872
111	1	8.34816326530612
111	2	4.162120181405898
111	3	4.07736961451247
111	4	7.681031746031746
111	5	9.348945578231294
111	6	4.392414965986397
111	7	10.651689342403628
111	8	3.412573696145138
111	9	5.9475736961451275
111	10	8.778854875283447
111	11	-0.4310770975056677
111	12	4.083605442176864
111	13	7.702687074829928
111	14	10.216213151927434
111	15	-2.579943310657594
111	16	2.149285714285707
111	17	2.083469387755102
111	18	-6.652777777777773
111	19	-8.790464852607712
111	20	-9.350306122448977
111	21	-7.363956916099774
111	22	-6.836054421768706
111	23	-2.3303061224489756
111	24	-8.827596371882086
111	25	-8.316700680272104
111	26	-6.086009070294784
111	27	-6.0874829931972805
111	28	-5.61960317460318
111	29	0.28465986394557774
111	30	-5.430090702947847
111	31	-5.902868480725623
111	32	0.9781859410430869
111	33	1.7825396825396815
111	34	2.737199546485259
111	35	1.581031746031746
111	36	3.008854875283445
111	37	3.0362585034013563
111	38	2.4350453514739225
111	39	2.816802721088436
111	40	3.1263945578231276
112	1	-7.65183673469388
112	2	-5.00454648526077
112	3	-10.42263038548753
112	4	-3.3189682539682543
112	5	-8.484387755102038
112	6	-9.607585034013603
112	7	-3.181643990929704
112	8	-8.087426303854862
112	9	-3.3857596371882046
112	10	-7.887811791383221
112	11	-3.264410430839
112	12	-15.249727891156468
112	13	-13.297312925170072
112	14	-11.783786848072566
112	15	-12.413276643990926
112	16	-11.850714285714293
112	17	-1.7498639455782303
112	18	-1.8194444444444393
112	19	1.3762018140589554
112	20	-0.850306122448977
112	21	1.3027097505668923
112	22	-11.16938775510204
112	23	-11.66363945578231
112	24	-11.827596371882086
112	25	-10.316700680272104
112	26	-9.086009070294784
112	27	-9.08748299319728
112	28	-8.61960317460318
112	29	-8.715340136054422
112	30	-8.430090702947847
112	31	-8.902868480725623
112	32	-8.021814058956913
112	33	-7.2174603174603185
112	34	-6.262800453514741
112	35	-6.752301587301588
112	36	-5.991145124716555
112	37	-5.963741496598644
112	38	-5.5649546485260775
112	39	-5.183197278911564
112	40	-4.873605442176872
115	1	-3.6518367346938803
115	2	2.4954535147392303
115	3	-2.9226303854875297
115	4	-1.3189682539682543
115	5	-5.984387755102038
115	6	11.392414965986397
115	7	-6.681643990929704
115	8	12.412573696145138
115	9	-15.385759637188205
115	10	-12.887811791383221
115	11	-17.764410430839
115	12	-17.249727891156468
115	13	-18.297312925170072
115	14	-18.783786848072566
115	15	-16.913276643990926
115	16	-14.850714285714293
115	17	-14.24986394557823
115	18	-15.31944444444444
115	19	-12.123798185941045
115	20	-13.350306122448977
115	21	-11.697290249433108
115	22	-11.16938775510204
115	23	-11.66363945578231
115	24	-11.827596371882086
115	25	-10.316700680272104
115	26	-9.086009070294784
115	27	-9.08748299319728
115	28	-8.61960317460318
115	29	-8.715340136054422
115	30	-8.430090702947847
115	31	-8.902868480725623
115	32	-8.021814058956913
115	33	-7.2174603174603185
115	34	-6.262800453514741
115	35	-6.752301587301588
115	36	-5.991145124716555
115	37	-5.963741496598644
115	38	-5.5649546485260775
115	39	-5.183197278911564
115	40	-4.873605442176872
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
4	540.0
5	19.0
6	23.0
7	28.0
8	32.0
9	44.0
10	83.0
11	120.0
12	121.0
13	119.0
14	128.0
15	139.0
16	161.0
17	149.0
18	152.0
19	161.0
20	161.0
21	166.0
22	198.0
23	177.0
24	159.0
25	156.0
26	117.0
27	112.0
28	73.0
29	51.0
30	31.0
31	15.0
32	7.0
33	3.0
34	2.0
35	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	0.0	0.0	100.0
3	0.0	0.0	0.0	100.0
4	0.0	0.0	100.0	0.0
5	100.0	0.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	99.53555878084181	0.2612481857764877	0.029027576197387515	0.1741654571843251
8	98.43342036553526	0.4641717435451117	0.17406440382941687	0.9283434870902234
9	97.58931164681964	1.1617775196049955	0.6099331977926228	0.6389776357827476
10	32.36658932714617	39.646171693735496	11.687935034802784	16.299303944315547
11	24.013921113689097	34.077726218097446	22.36078886310905	19.54756380510441
12	38.352190310414855	17.087322309254425	21.35190020307514	23.208587177255584
13	27.204176334106727	20.33062645011601	25.5800464037123	26.885150812064968
14	22.679814385150813	28.42227378190255	27.610208816705335	21.2877030162413
15	24.738979118329464	26.62412993039443	26.59512761020882	22.041763341067284
16	23.75290023201856	22.0707656612529	28.48027842227378	25.696055684454755
17	26.479118329466356	26.740139211136892	20.272621809744777	26.50812064965197
18	27.030162412993043	27.001160092807424	21.72273781902552	24.245939675174014
19	19.737991266375545	18.020378457059678	15.749636098981076	46.49199417758369
20	24.042923433874712	28.016241299303946	20.446635730858468	27.494199535962878
21	22.940835266821345	22.2737819025522	23.54988399071926	31.235498839907194
22	23.179576443284013	19.843342036553523	21.78706121264868	35.190020307513784
23	26.740139211136892	21.983758700696054	25.319025522041766	25.95707656612529
24	19.721577726218097	24.564965197215777	25.116009280742457	30.59744779582367
25	22.364217252396166	25.588149869300032	25.29770548939878	26.749927388905025
26	20.955989507432236	23.141941125036432	29.87467210725736	26.027397260273972
27	18.445475638051047	19.54756380510441	33.03364269141532	28.973317865429234
28	16.1589788221642	20.74267478967218	36.0313315926893	27.067014795474325
29	15.26407428903076	19.326755658734765	36.9413813116657	28.46778874056878
30	15.529753265602322	17.503628447024674	40.696661828737305	26.2699564586357
31	13.957545798197149	21.634195987205583	44.54783367257924	19.86042454201803
32	17.546403712296986	14.907192575406034	44.3445475638051	23.201856148491878
33	12.325986078886311	16.61832946635731	48.085846867749424	22.96983758700696
34	12.064965197215777	15.980278422273782	52.37819025522042	19.576566125290025
35	13.138051044083527	17.40139211136891	48.75290023201856	20.707656612529
36	9.338747099767982	17.28538283062645	49.47795823665893	23.897911832946637
37	8.932714617169374	15.690255220417635	50.55104408352668	24.825986078886313
38	10.440835266821345	17.633410672853827	49.129930394431554	22.795823665893273
39	12.15197215777262	16.87935034802784	49.593967517401396	21.374709976798144
40	10.382830626450117	21.46171693735499	48.98491879350348	19.170533642691414
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	3.5
17	7.0
18	7.0
19	9.5
20	12.0
21	22.0
22	32.0
23	32.0
24	42.0
25	52.0
26	62.5
27	73.0
28	73.0
29	71.0
30	69.0
31	86.5
32	104.0
33	104.0
34	129.0
35	154.0
36	172.0
37	190.0
38	190.0
39	225.5
40	261.0
41	267.0
42	273.0
43	273.0
44	261.0
45	249.0
46	247.5
47	246.0
48	246.0
49	229.0
50	212.0
51	197.5
52	183.0
53	183.0
54	180.0
55	177.0
56	182.5
57	188.0
58	188.0
59	192.5
60	197.0
61	191.0
62	185.0
63	185.0
64	179.5
65	174.0
66	157.0
67	140.0
68	140.0
69	129.0
70	118.0
71	99.0
72	80.0
73	80.0
74	59.0
75	38.0
76	27.5
77	17.0
78	17.0
79	14.5
80	12.0
81	7.5
82	3.0
83	3.0
84	2.5
85	2.0
86	1.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.08700696055684454
8	0.029002320185614848
9	0.14501160092807425
10	0.0
11	0.0
12	0.029002320185614848
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.37703016241299303
20	0.0
21	0.0
22	0.029002320185614848
23	0.0
24	0.0
25	0.14501160092807425
26	0.4930394431554524
27	0.0
28	0.029002320185614848
29	0.058004640371229696
30	0.08700696055684454
31	0.2610208816705336
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
40	3448.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.18561484918794
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.67885435709933	92.02436194895591
2	2.46800731261426	4.698375870069606
3	0.4875076173065204	1.3921113689095126
4	0.1218769043266301	0.46403712296983757
5	0.09140767824497258	0.43503480278422274
6	0.09140767824497258	0.5220417633410672
7	0.0	0.0
8	0.06093845216331505	0.46403712296983757
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCTGAGGGCTCGTCTTACTCAGCAACATCTTTTGATACA	8	0.23201856148491878	No Hit
ACCTGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT	8	0.23201856148491878	No Hit
ACCTGAGGGCTCGTCTTAATCAGCAACATCTTTTGATACA	6	0.17401392111368907	No Hit
ACCTGAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT	6	0.17401392111368907	No Hit
ACCTGAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT	6	0.17401392111368907	No Hit
ACCTGAGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAA	5	0.14501160092807425	No Hit
ACCTGAGGGAAGTGTGTGGGTTGTTAGACTATTTCTCCCT	5	0.14501160092807425	No Hit
ACCTGAGGGGCCGAATAGCCTCTCCACCCAAGCGGCCCGA	5	0.14501160092807425	No Hit
ACCTGAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT	4	0.11600928074245939	No Hit
ACCTGAGGGGCCGAATAGCCTCTCCACCCCAGCGGCCGGA	4	0.11600928074245939	No Hit
ACCTGAGGGAGTCGACTTCTTTTTTTTTTTTTTTTTTTTT	4	0.11600928074245939	No Hit
ACCTGAGGGACTCTTTCAGGTCACTCTGGGGCTGCCTCTT	4	0.11600928074245939	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.029002320185614848	0.0	0.0	0.0	0.0
26	0.029002320185614848	0.0	0.0	0.0	0.0
27	0.029002320185614848	0.0	0.0	0.0	0.0
28	0.029002320185614848	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGGAAG	50	1.7826096E-10	34.298508	6
GGAAGCA	20	0.002311027	34.298508	8
TGAGGGT	35	6.751561E-7	34.298508	4
TGAGGGG	100	0.0	34.298508	4
GGGAAGT	20	0.002311027	34.298508	7
GGGAAGC	25	1.5483066E-4	34.298508	7
GAGGGGT	30	1.0276313E-5	34.298508	5
GAGGGGC	30	1.0276313E-5	34.298508	5
GAGGGGA	30	1.0276313E-5	34.298508	5
GAGGGCT	25	1.5483066E-4	34.298508	5
GAGGGCA	20	0.002311027	34.298508	5
GAGGGAT	30	1.0276313E-5	34.298508	5
GAGGGAC	25	1.5483066E-4	34.298508	5
GAGGGAA	55	1.0913936E-11	34.298508	5
CTGAGGG	335	0.0	33.274673	3
TGAGGGA	130	0.0	32.979332	4
ACCTGAG	350	0.0	32.82857	1
CCTGAGG	345	0.0	32.81458	2
TGAGGGC	70	0.0	31.848616	4
GAGGGCC	25	0.0068960926	27.438807	5
>>END_MODULE