FastQCFastQC Report
Fri 17 Jun 2016
SRR1525935_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525935_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3938
Sequences flagged as poor quality0
Sequence length40
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTGAGGGAGATTGGGCGGATTGAACAAAAACTCATCTC691.7521584560690706No Hit
ACCTGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC591.4982224479431183No Hit
ACCTGAAGGAGATTGGGCGGATTGAACAAAAACTCATCTC501.2696800406297613No Hit
ACCTGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT431.0919248349415946No Hit
ACCTGAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT380.9649568308786186No Hit
ACCTGAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG320.8125952260030471No Hit
ACCTGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG280.7110208227526663No Hit
ACCTGAGGGCGATTGGGCGGATTGAACAAAAACTCATCTC130.33011681056373793No Hit
ACCTGAGGGAGATTGGGAGGATTGAACAAAAACTCATCTC90.22854240731335704No Hit
ACCTGAGGGGGGTGACGGGGAATCAGGGTTCGATTCCGGA80.20314880650076178No Hit
ACCTGAGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCAC70.17775520568816658No Hit
ACCTGAGGGGAACGTCTGCCCTATCAACTTTCGATGGTAG70.17775520568816658No Hit
ACCTGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGA60.15236160487557138No Hit
ACCTGAGGGAGATTGGGCGGATTGAACAAAAACTCATATC50.12696800406297612No Hit
ACCTGAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGC50.12696800406297612No Hit
ACCTGAGGGTCACAGATCCTCTTCTGAGATGCGTTTTTGT50.12696800406297612No Hit
ACCTGAGGGAGTGAAACTGCGAATGGCTCATTAAATCAGT50.12696800406297612No Hit
ACCTGACGGCGATTGGGCGGATTGAACAAAAACTCATCTC50.12696800406297612No Hit
ACCTGAGGGGGGATGGTGAGATGGCTCAGTGGGTAAGAGC50.12696800406297612No Hit
ACCTGAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCA50.12696800406297612No Hit
ACCTGAAGGAGATTGGGAGGATTGAACAAAAACTCATCTC50.12696800406297612No Hit
ACCTGAGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT50.12696800406297612No Hit
ACCTGAGGGGGGCTGGTGAGCTGGCTCAGTGGGTAAGAGC50.12696800406297612No Hit
ACCTGAGGGTACCTGGTTGATACTGCCAGTAGCATATGCT50.12696800406297612No Hit
ACCTGAGGGGGGGAGGTAGTGACGAAAAATAACAATACAG50.12696800406297612No Hit
ACCTGACGGAGATTGGGCGGATTGAACAAAAACTCATCTC50.12696800406297612No Hit
ACCTGAGGGCAGTGCTACCTGAGGGTCACAGATCCTCTTC40.10157440325038089No Hit
ACCTGAGGGTCTCTAATATTCACAGGAGAAAGTGGAGGAT40.10157440325038089No Hit
ACCTGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGA40.10157440325038089No Hit
ACCTGAGGGAGATTGGGCGGATTGAACAAAAACTCATCTT40.10157440325038089No Hit
ACCTGAGGGAGATTGGGCGGATTGAACAAAAACTCATCTG40.10157440325038089No Hit
ACCTGAGGGATCTGGGGTTATCTGGGTCTTCAGTACTCTG40.10157440325038089No Hit
ACCTGAGGGCTCTCCTACTTGGATAACTGTGGTAATTCTA40.10157440325038089No Hit
ACCTGAAGGAGATTGGGCGGATTGAACAAAAACTAATCTC40.10157440325038089No Hit
ACCTGAGGGAATGTTGAGTCTCACCATGGCAACTCTGTTT40.10157440325038089No Hit
ACCTGAGGGAGCATATGCTTGTCTCAAAGATTAAGCCATG40.10157440325038089No Hit
ACCTGAGGGGATTCCGGAGAGGGAGCCTGAGAAACGGCTA40.10157440325038089No Hit
ACCTGAGGGGTGCTACCTGAGGGTCACAGATCCTCTTCTG40.10157440325038089No Hit
ACCTGAGGGGTTGGTGGTGTCCATGCTGAAGTTCTAACTG40.10157440325038089No Hit
ACCTGAGGGACCCACTCCCGACCCGGGGAGGTAGTGACGA40.10157440325038089No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTCTT301.1411596E-534.017
AGGGGGC200.002463436534.06
ACAAAAA200.002463436534.026
AAACTCA357.680046E-734.030
AACAAAA200.002463436534.025
CTCTTCT301.1411596E-534.019
GGATTGA301.1411596E-534.019
CGGATTG301.1411596E-534.018
CCTGAAG251.682555E-434.02
GCGGATT357.680046E-734.017
AGGGATT200.002463436534.06
GATTGAA200.002463436534.020
AGGGACA200.002463436534.06
ACTCATC301.1411596E-534.032
ACCTGAA251.682555E-434.01
CCTCTTC301.1411596E-534.018
TCTGAAG251.682555E-434.023
ATTGAAC200.002463436534.021
TGAGGGC357.680046E-734.04
AAAACTC357.680046E-734.029
Produced by FastQC (version 0.11.5)