FastQCFastQC Report
Fri 17 Jun 2016
SRR1525934_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525934_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20273
Sequences flagged as poor quality0
Sequence length56
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCGGAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT3291.6228481231194198No Hit
TGCGGAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC2721.34168598628718No Hit
TGCGGAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC1030.5080649139249248No Hit
TGCGGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG740.36501751097518864No Hit
TGCGGAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT550.27129679869777534No Hit
TGCGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC500.24663345336161396No Hit
TGCGGAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA500.24663345336161396No Hit
TGCGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC390.19237409362205887No Hit
TGCGGAGGGTCACAGATCCTCTGAAGGAGATTGGGCGGATTGAACAAAAACTCATC270.13318206481527153No Hit
TGCGGAGGGAGCCTGCCATCCCATTTGGATGTATCAATGCCACTGAGTTGTCTGTC270.13318206481527153No Hit
TGCGGAGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT250.12331672668080698No Hit
TGCGGAGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA240.1183840576135747No Hit
TGCGGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG240.1183840576135747No Hit
TGCGGAGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT240.1183840576135747No Hit
TGCGGAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGACCAAAAACTC230.11345138854634243No Hit
TGCGGAGGGTCACAGATCCTCTCCTGAAGGAGATTGGGCGGATTGAACAAAAACTC220.10851871947911015No Hit
TGCGGAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCTAATCCGCCCAATCT220.10851871947911015No Hit
TGCGGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT210.10358605041187786No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTGAA203.8286168E-450.6599541
AAAGATT203.8286168E-450.6599547
GACACAA150.00789047650.65994645
ACACAAA150.00789047650.65994646
TAATCCG150.00796963350.5326642
CATCCGC150.00804937950.40601343
GCGCATT204.0237757E-450.15461738
CGCATTG204.0237757E-450.15461739
GCATTGA204.0237757E-450.15461740
CAACAAA150.00821065250.15461340
ACAACAA150.00821065250.15461339
TTTTGTC309.928517E-750.0298535
TTGTCTA150.00829218250.0298537
TGGTTGA252.007398E-550.0298514
GGTTGAT252.007398E-550.0298515
GCCCAAG150.00829218250.0298548
GGCGCAT204.073779E-450.0298537
TAGCATA204.073779E-450.0298530
AAGATTA204.073779E-450.0298548
AGCATAT204.073779E-450.0298531
Produced by FastQC (version 0.11.5)