##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1525934_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20273
Sequences flagged as poor quality	0
Sequence length	56
%GC	54
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.61658363340403	39.0	37.0	39.0	33.0	40.0
2	35.959354806886004	38.0	35.0	39.0	30.0	40.0
3	35.877916440586	38.0	35.0	39.0	29.0	40.0
4	35.617027573620085	38.0	35.0	39.0	28.0	40.0
5	35.29206333547082	38.0	35.0	39.0	27.0	40.0
6	32.95619789868298	38.0	33.0	39.0	13.0	40.0
7	35.660829674937105	38.0	35.0	39.0	29.0	40.0
8	35.60923395649386	38.0	35.0	39.0	29.0	40.0
9	34.52197504069452	38.0	35.0	39.0	25.0	40.0
10	33.78429438168993	37.0	32.0	39.0	22.0	40.0
11	33.68208947861688	38.0	32.0	39.0	23.0	40.0
12	33.1589799240369	38.0	31.0	39.0	20.0	40.0
13	32.584965224683074	36.0	30.0	39.0	18.0	40.0
14	33.21693878557688	38.0	32.0	39.0	19.0	40.0
15	31.764662358802347	36.0	29.0	39.0	15.0	40.0
16	32.37636264982982	36.0	30.0	38.0	19.0	40.0
17	31.406353277758594	35.0	28.0	38.0	15.0	40.0
18	30.903023726138212	35.0	27.0	38.0	14.0	40.0
19	32.20120357125241	36.0	29.0	38.0	19.0	40.0
20	32.61258817145958	36.0	30.0	38.0	21.0	40.0
21	32.998224239135794	37.0	31.0	39.0	20.0	40.0
22	32.09189562472254	36.0	29.0	39.0	16.0	40.0
23	32.91550337887831	37.0	31.0	39.0	19.0	40.0
24	32.14955852611848	36.0	30.0	38.0	17.0	40.0
25	32.62378533024219	36.0	31.0	38.0	19.0	40.0
26	32.547279633009424	36.0	31.0	39.0	18.0	40.0
27	32.76066689685789	37.0	31.0	39.0	19.0	40.0
28	33.03018793469146	37.0	32.0	39.0	20.0	40.0
29	32.26868248409214	36.0	30.0	38.0	17.0	40.0
30	32.27682138805307	36.0	30.0	38.0	17.0	40.0
31	32.79637942090465	36.0	32.0	39.0	18.0	40.0
32	32.8028905440734	37.0	32.0	38.0	18.0	40.0
33	32.504908005721894	36.0	31.0	38.0	18.0	39.0
34	32.137078873378385	36.0	31.0	38.0	16.0	39.0
35	32.460020717210085	36.0	32.0	38.0	17.0	40.0
36	31.78533024219405	36.0	31.0	38.0	10.0	39.0
37	31.640408424998768	36.0	30.0	38.0	4.0	39.0
38	30.985054012726287	35.0	30.0	38.0	4.0	39.0
39	29.66867261875401	35.0	27.0	38.0	4.0	39.0
40	27.765698219306465	33.0	19.0	38.0	4.0	39.0
41	25.64682089478617	31.0	14.0	37.0	4.0	39.0
42	26.040201252897944	31.0	17.0	36.0	4.0	39.0
43	26.57292951215903	32.0	18.0	37.0	4.0	39.0
44	25.71883786316776	31.0	14.0	37.0	4.0	39.0
45	26.490652592117595	32.0	18.0	37.0	4.0	39.0
46	24.186356237360034	29.0	10.0	36.0	4.0	39.0
47	23.53771025501899	28.0	7.0	35.0	4.0	38.0
48	23.906180634341244	28.0	9.0	35.0	4.0	38.0
49	22.993143589996546	27.0	4.0	35.0	4.0	38.0
50	23.772012035712525	29.0	4.0	35.0	4.0	38.0
51	24.051151778227197	30.0	4.0	36.0	4.0	39.0
52	23.771666748877816	30.0	4.0	36.0	4.0	39.0
53	23.70734474424111	30.0	4.0	36.0	4.0	38.0
54	23.116460316677355	29.0	4.0	35.0	4.0	38.0
55	22.98880284121738	29.0	4.0	36.0	4.0	38.0
56	22.17136092339565	28.0	4.0	35.0	4.0	38.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	2.5661730717169604
1	2	4.019443442439176
1	3	4.5594983099797375
1	4	4.933534896354644
1	5	5.138531894401957
1	6	7.495177188176989
1	7	4.597228467765504
1	8	4.485806685677588
1	9	5.054934057361983
1	10	6.087807224358425
1	11	-0.7420097272469341
1	12	-6.896023673196602
1	13	-17.8748038275409
1	14	-6.638686155932387
1	15	0.8093690205583535
1	16	-3.0734733827420264
1	17	-5.986325812300141
1	18	-5.058873779783472
1	19	0.1233999258513343
1	20	-2.1245906874395217
1	21	4.939116842390675
1	22	6.156170362390284
1	23	5.41966231385662
1	24	5.426941211495244
1	25	5.1920844172603005
1	26	5.423018517448384
1	27	4.2681451555976615
1	28	4.731496234950107
1	29	5.885117490191682
1	30	4.919387147802954
1	31	2.5252870892144443
1	32	2.9151141190922374
1	33	2.778841791092507
1	34	2.4491762410772715
1	35	3.3590504706812716
1	36	2.626003118609944
1	37	2.5960013007955354
1	38	4.046619708935054
1	39	2.7612150633099617
1	40	-12.777631400613492
1	41	-1.9330076328520391
1	42	-4.0358382106063395
1	43	-8.51865820240727
1	44	-15.28275590729905
1	45	-2.3667847051213364
1	46	-5.34499508682079
1	47	-9.682635388867636
1	48	-4.897061482065784
1	49	-13.352124508635253
1	50	-3.94524429465892
1	51	2.7411274394715015
1	52	2.32598610619608
1	53	2.5507784507884814
1	54	3.5743609821483666
1	55	3.197930267018137
1	56	6.3920968201998605
2	1	3.8659566215005157
2	2	4.945850368846102
2	3	4.856035106516529
2	4	5.502798965618709
2	5	5.431821937692
2	6	7.376129569129368
2	7	4.912163532700568
2	8	4.84727854714945
2	9	5.615540117968045
2	10	5.984993371544572
2	11	4.933314948077744
2	12	0.44704991987699216
2	13	1.4639407612036877
2	14	5.771487004240775
2	15	6.2552564664458
2	16	3.881072071803427
2	17	6.273414447440121
2	18	4.356710635800944
2	19	6.0000233024747125
2	20	3.6297383168894832
2	21	6.60253675581059
2	22	8.313096769316694
2	23	7.037627681821988
2	24	7.488629523183551
2	25	7.194248919424805
2	26	7.222802067231939
2	27	7.192387579840084
2	28	6.221755975209845
2	29	7.474944330018523
2	30	6.350123078538889
2	31	4.967927781855138
2	32	5.284161738139858
2	33	5.298322310573031
2	34	4.818223860124892
2	35	4.808184669815471
2	36	6.511284503891332
2	37	7.417429872224105
2	38	8.128870791186138
2	39	7.750392552487451
2	40	6.201805828823737
2	41	10.544265094420687
2	42	8.990135815367687
2	43	7.382856949107882
2	44	-2.3087299332730744
2	45	8.777154688818054
2	46	8.38444214261644
2	47	6.661520455288208
2	48	7.249042414038115
2	49	-0.35320675971750504
2	50	8.646747047332422
2	51	11.280088478432539
2	52	11.557587837797811
2	53	11.561600961610992
2	54	11.69990210768949
2	55	10.859185678273548
2	56	13.164824092927134
3	1	2.8280778336217267
3	2	3.817062490058227
3	3	4.277752278233706
3	4	4.4295666423863835
3	5	4.618690624560685
3	6	6.4872406802404825
3	7	4.275799896336935
3	8	4.215965415836315
3	9	5.221600724028647
3	10	5.909235795786994
3	11	4.781799796562588
3	12	4.707150929977999
3	13	5.1331326803956046
3	14	5.412901145654914
3	15	-1.1311071699178363
3	16	6.315415506146859
3	17	6.700182124207796
3	18	7.861761140851449
3	19	5.659114211565619
3	20	4.41509185224302
3	21	5.923243826517663
3	22	5.775217981437905
3	23	6.042678186872493
3	24	6.672972957526984
3	25	5.5452590204349015
3	26	3.93492327935315
3	27	3.6292562667087758
3	28	5.044988298442171
3	29	3.3057524108266065
3	30	4.4233554017712144
3	31	3.7356045495318995
3	32	3.6730506270287435
3	33	3.374079886330602
3	34	3.4015571934582205
3	35	4.795558407189208
3	36	5.657749150355976
3	37	5.7507632055574405
3	38	5.606143518458865
3	39	6.300897602992503
3	40	7.519987647005557
3	41	11.261436811592404
3	42	8.86892369415557
3	43	9.925786242037173
3	44	9.701371076827936
3	45	9.994326405989778
3	46	10.619290627464924
3	47	12.52768207144982
3	48	11.150557565553267
3	49	10.846288189777447
3	50	10.411898562483938
3	51	10.237159185503248
3	52	9.282335312545285
3	53	10.261095911105944
3	54	8.391821299608683
3	55	7.995549314637184
3	56	7.058763486866528
4	1	3.744744500288391
4	2	4.733729156724891
4	3	4.510391167122592
4	4	4.946927753497498
4	5	5.299246180116242
4	6	7.959462902462704
4	7	4.807049896336935
4	8	4.3652709713918725
4	9	5.679934057361983
4	10	5.877985795786994
4	11	5.3963831298959235
4	12	6.380762041089113
4	13	6.035910458173383
4	14	6.030956701210471
4	15	5.545976163415496
4	16	6.832776617257974
4	17	7.6967099019855745
4	18	8.30273336307367
4	19	6.534114211565619
4	20	5.647730741131905
4	21	7.180188270962105
4	22	7.254384648104569
4	23	6.44198374242805
4	24	4.877834068638098
4	25	7.312620131546016
4	26	5.195339946019814
4	27	3.5493951555976615
4	28	4.142210520664392
4	29	5.527974633048828
4	30	5.1872442906601
4	31	5.409215660643014
4	32	4.107078404806522
4	33	4.0511632196639376
4	34	4.547390526791556
4	35	4.010836184966987
4	36	4.92511026146709
4	37	5.792429872224105
4	38	4.5332268517922
4	39	6.971036491881389
4	40	1.9991543136722214
4	41	9.03574236714796
4	42	9.035590360822233
4	43	8.29384179759273
4	44	10.489565521272379
4	45	5.539465294878664
4	46	9.963040627464924
4	47	10.045043182560935
4	48	9.598474232219932
4	49	10.19698263422189
4	50	10.380648562483938
4	51	9.75452029661436
4	52	9.160807534767507
4	53	9.340957022217054
4	54	9.065432410719794
4	55	10.443465981303852
4	56	8.48584682019986
5	1	3.432244500288391
5	2	4.483729156724891
5	3	3.9791411671225916
5	4	5.009427753497498
5	5	4.861746180116242
5	6	6.959462902462704
5	7	4.775799896336935
5	8	4.3965209713918725
5	9	5.461184057361983
5	10	5.409235795786994
5	11	5.4901331298959235
5	12	6.287012041089113
5	13	5.567160458173383
5	14	5.874706701210471
5	15	6.264726163415496
5	16	6.239026617257974
5	17	6.9779599019855745
5	18	9.05273336307367
5	19	8.065364211565619
5	20	5.647730741131905
5	21	6.680188270962105
5	22	7.535634648104569
5	23	6.56698374242805
5	24	7.127834068638098
5	25	7.375120131546016
5	26	5.132839946019814
5	27	4.9868951555976615
5	28	5.673460520664392
5	29	5.840474633048828
5	30	5.4372442906601
5	31	4.534215660643014
5	32	5.482078404806522
5	33	5.1136632196639376
5	34	5.203640526791556
5	35	5.104586184966987
5	36	5.61261026146709
5	37	6.636179872224105
5	38	6.5644768517922
5	39	7.783536491881389
5	40	8.842904313672221
5	41	10.75449236714796
5	42	9.785590360822233
5	43	10.88759179759273
5	44	11.458315521272379
5	45	10.539465294878664
5	46	12.713040627464924
5	47	12.763793182560935
5	48	12.004724232219932
5	49	13.16573263422189
5	50	12.786898562483938
5	51	11.75452029661436
5	52	12.410807534767507
5	53	12.872207022217054
5	54	12.690432410719794
5	55	11.943465981303852
5	56	12.23584682019986
6	1	3.9060348228690387
6	2	4.5724388341442435
6	3	4.94889923163872
6	4	5.54874226962653
6	5	5.674246180116242
6	6	8.419140321817544
6	7	5.308057960853063
6	8	5.303779035908001
6	9	6.006546960587791
6	10	7.118913215141834
6	11	6.3731976460249555
6	12	6.729552363669761
6	13	7.386716909786287
6	14	6.8878115399201505
6	15	7.726419711802592
6	16	7.6523330688708775
6	17	8.308605063275895
6	18	8.148499492105927
6	19	7.102662598662395
6	20	6.666883966938354
6	21	5.956397948381458
6	22	7.0628523900400495
6	23	6.162749871460306
6	24	6.808277617025194
6	25	6.63217658315892
6	26	5.327396397632718
6	27	4.5100806394686295
6	28	5.283339552922456
6	29	5.61668431046818
6	30	6.6590184842084845
6	31	3.1047801767720458
6	32	3.660505824161362
6	33	4.04612289708329
6	34	3.871987300985108
6	35	4.484626507547635
6	36	5.333376390499346
6	37	5.256139549643457
6	38	5.447541367921232
6	39	6.431721975752357
6	40	4.493105926575446
6	41	10.23634720585764
6	42	8.954945199531913
6	43	10.199083733076602
6	44	10.920009069659475
6	45	10.28644110133028
6	46	11.869290627464924
6	47	13.098470601915775
6	48	11.841417780607028
6	49	12.75041005357673
6	50	13.12964049796781
6	51	12.872463845001455
6	52	11.48036398638041
6	53	12.39640057060415
6	54	13.164222733300441
6	55	13.61382888452966
6	56	12.021129078264376
7	1	3.193020362357359
7	2	4.319936053276614
7	3	4.571813580915695
7	4	4.669988098325085
7	5	5.225970318047274
7	6	7.812911178324775
7	7	3.51717920668176
7	8	4.443934764495317
7	9	4.89976164356888
7	10	5.374753037166304
7	11	6.218581405757995
7	12	6.568262041089113
7	13	6.4712552857595895
7	14	5.330525666727709
7	15	7.607398577208599
7	16	7.047216272430386
7	17	7.693477143364884
7	18	8.704673018246083
7	19	8.184976280531135
7	20	6.295360051476731
7	21	6.6694124088931375
7	22	7.433263958449395
7	23	6.408578570014257
7	24	5.137532344500169
7	25	6.4753356487873965
7	26	4.480900290847401
7	27	4.2164210176666295
7	28	5.18100362411267
7	29	6.467629805462622
7	30	5.3208649803152746
7	31	3.226026005470601
7	32	4.606000818599625
7	33	3.241895978284628
7	34	4.795235354377763
7	35	5.392301702208368
7	36	5.441274054570535
7	37	5.555360906706866
7	38	7.5687871966197875
7	39	5.241510629812421
7	40	8.341826727465325
7	41	10.058371677492786
7	42	10.259728291856717
7	43	9.16237628035135
7	44	11.111332762651688
7	45	10.141835984533838
7	46	13.455497524016646
7	47	14.354310423940245
7	48	13.343086301185448
7	49	14.14418091008396
7	50	12.851553734897731
7	51	9.822408227648843
7	52	9.668350638215784
7	53	9.93255184980326
7	54	10.38224275554738
7	55	10.610491843372817
7	56	10.374855440889515
8	1	1.3601291156730042
8	2	2.8491137721095043
8	3	3.3397180901995114
8	4	3.944523907343651
8	5	5.020400026270089
8	6	7.132539825539624
8	7	3.045030665567701
8	8	4.425367125238026
8	9	4.478010980438903
8	10	4.640005026556228
8	11	3.5382100529728433
8	12	4.760569733396807
8	13	5.50706430432723
8	14	5.045379778133551
8	15	6.413764624953956
8	16	5.77268046341182
8	17	7.612575286600961
8	18	6.660906439996751
8	19	6.524498826950232
8	20	5.900134587285752
8	21	5.963842117115952
8	22	5.768807725027649
8	23	5.367464511658817
8	24	5.726391760945791
8	25	4.367908593084476
8	26	4.178513022942894
8	27	3.921991309443815
8	28	4.5364412898951585
8	29	2.893359248433441
8	30	3.1007058291216403
8	31	4.385177199104554
8	32	4.412366866344982
8	33	4.245874758125478
8	34	3.369505911406943
8	35	2.1310284926592935
8	36	3.2303987230055498
8	37	4.186660641454871
8	38	4.997169159484507
8	39	6.497478799573695
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116	19	4.486037288488699
116	20	4.6309038180549855
116	21	4.963842117115952
116	22	5.422653878873803
116	23	3.52131066550497
116	24	5.611007145561178
116	25	2.6371393623152493
116	26	2.2554360998659675
116	27	1.8066066940592016
116	28	2.5364412898951585
116	29	2.393359248433441
116	30	4.0622442906601
116	31	3.423638737566094
116	32	2.7200591740372886
116	33	3.899720911971631
116	34	2.1387366806377095
116	35	1.9387208003516
116	36	5.153475646082477
116	37	5.225122179916411
116	38	4.72793839025374
116	39	4.343632645727542
116	40	6.2251158521337615
116	41	6.355453905609501
116	42	4.38174420697608
116	43	0.15441872066965345
116	44	-1.315722940266081
116	45	4.72936914103251
116	46	2.753906012080307
116	47	-3.611206817439065
116	48	0.22107038606608498
116	49	0.9253480188372727
116	50	1.7388216394070142
116	51	4.867501065845129
116	52	5.216095996305967
116	53	5.083745483755514
116	54	4.195240103027487
116	55	4.239139058226929
116	56	5.3920968201998605
117	1	2.9026392371304937
117	2	3.838992314619631
117	3	4.127167482912064
117	4	4.395940911392238
117	5	4.726877759063612
117	6	7.604199744567964
117	7	3.960010422652722
117	8	4.429415708233975
117	9	4.502302478414613
117	10	4.777656848418577
117	11	0.1151331298959235
117	12	-5.221211643121414
117	13	-0.786457962879247
117	14	-0.5841748777368991
117	15	-3.100405415531874
117	16	-11.678736540636763
117	17	-2.008882203277583
117	18	-0.7581219000842232
117	19	0.9617457905129889
117	20	-1.577598206236516
117	21	4.380846165698948
117	22	5.560305700736151
117	23	4.20349690032279
117	24	4.40250512126968
117	25	5.248475394703913
117	26	4.101589946019814
117	27	2.557618839808189
117	28	3.819842099611762
117	29	4.304290422522513
117	30	4.035928501186415
117	31	3.0046103974851164
117	32	3.3192494574381044
117	33	2.6810974301902526
117	34	2.4322589478441863
117	35	4.691757237598569
117	36	4.479386577256562
117	37	5.89111408275042
117	38	5.610529483371145
117	39	6.742418070828759
117	40	9.897180629461694
117	41	3.869623946095331
117	42	1.8513798345064423
117	43	-6.610763465565164
117	44	-4.012079215569727
117	45	2.962162663299715
117	46	-2.078077793587706
117	47	-2.3480489227022225
117	48	-0.6169862940958595
117	49	-3.538214734199162
117	50	1.42505645722078
117	51	6.150901875561729
117	52	6.254557534767507
117	53	5.832733338006527
117	54	5.213458726509266
117	55	5.635900191830167
117	56	8.628938925463018
118	1	2.692112921341021
118	2	3.312676525145946
118	3	3.3376937987015367
118	4	4.132783016655395
118	5	4.621614601168872
118	6	7.341041849831122
118	7	3.9073788437053523
118	8	3.95573149770766
118	9	4.607565636309353
118	10	4.935551585260679
118	11	1.0098699720011837
118	12	-5.484369537858257
118	13	-7.154879015510826
118	14	-6.952595930368478
118	15	-8.52145804711082
118	16	-9.310315488005184
118	17	0.20164411251188952
118	18	-5.758121900084223
118	19	-1.6698331568554323
118	20	-3.8933876799207248
118	21	2.275583007804208
118	22	3.4550425428414115
118	23	1.8877074266385776
118	24	3.507768279164413
118	25	3.6695280262828582
118	26	2.5752741565461292
118	27	3.2418293661239765
118	28	3.293526310138077
118	29	3.356922001469883
118	30	2.61487586960747
118	31	1.4782946080114314
118	32	1.3192494574381044
118	33	0.6284658512428862
118	34	1.1691010531073474
118	35	-0.09771644661196177
118	36	3.4267549983091925
118	37	3.4174298722241048
118	38	3.926318957055358
118	39	4.847681228723491
118	40	6.844549050514324
118	41	7.711729209253225
118	42	6.851379834506442
118	43	2.599762850224309
118	44	1.8826576265355364
118	45	7.593741610668136
118	46	1.6061327327280814
118	47	-0.9269962911232739
118	48	1.9619610743251954
118	49	-1.6434778920939017
118	50	2.951372246694465
118	51	7.571954507140674
118	52	7.570347008451716
118	53	8.253785969585476
118	54	8.10819556861453
118	55	9.372742297093325
118	56	12.20788629388407
119	1	2.3947445002883896
119	2	2.1337291567248897
119	3	2.266641167122593
119	4	3.590677753497495
119	5	3.5742461801162406
119	6	5.909462902462707
119	7	3.375799896336936
119	8	3.0715209713918696
119	9	3.60493405736198
119	10	4.859235795786997
119	11	-1.4348668701040772
119	12	-8.031737958910888
119	13	-3.589089541826617
119	14	-6.631543298789531
119	15	-5.7477738365845035
119	16	-5.773473382742026
119	17	1.2279599019855745
119	18	-5.266016636926331
119	19	-0.340885788434381
119	20	-1.7460192588680954
119	21	3.467688270962107
119	22	5.191884648104569
119	23	3.1482337424280473
119	24	2.334084068638095
119	25	3.206370131546013
119	26	4.801589946019817
119	27	5.218145155597664
119	28	5.217210520664395
119	29	5.9279746330488265
119	30	5.362244290660097
119	31	4.096715660643014
119	32	3.400828404806525
119	33	3.2574132196639347
119	34	5.334890526791558
119	35	5.2233361849669855
119	36	6.118860261467091
119	37	7.467429872224102
119	38	7.4894768517921975
119	39	9.339786491881387
119	40	10.636654313672224
119	41	8.56699236714796
119	42	8.185590360822232
119	43	-3.01865820240727
119	44	-0.30418447872762044
119	45	5.133215294878664
119	46	-0.3307093725350754
119	47	-5.861206817439065
119	48	-4.28277576778007
119	49	-4.259267365778111
119	50	1.511898562483939
119	51	6.448270296614361
119	52	7.204557534767506
119	53	7.072207022217054
119	54	5.752932410719794
119	55	7.462215981303853
119	56	11.042096820199859
120	1	2.2901990457429378
120	2	2.097365520361258
120	3	2.5984593489407715
120	4	2.927041389861131
120	5	2.674246180116242
120	6	7.209462902462704
120	7	3.6394362599733014
120	8	3.2260664259373257
120	9	4.28220678463471
120	10	4.0455994321506275
120	11	-1.06668505192226
120	12	-4.70446523163816
120	13	-5.384544087281164
120	14	-6.031543298789529
120	15	-0.45686474567541424
120	16	-5.43710974637839
120	17	0.7279599019855745
120	18	-2.097834818744513
120	19	-0.090885788434381
120	20	-0.4914738043226414
120	21	2.708597361871199
120	22	4.373702829922749
120	23	3.59823374242805
120	24	4.897720432274465
120	25	3.9063701315460158
120	26	5.283408127837994
120	27	3.040872428324935
120	28	3.4944832479371186
120	29	5.3688837239579215
120	30	3.3349715633873735
120	31	3.0739883879157404
120	32	2.450828404806522
120	33	2.798322310573031
120	34	1.530345072246103
120	35	1.4142452758760804
120	36	3.6779511705579964
120	37	2.871975326769558
120	38	4.325840488155833
120	39	5.280695582790482
120	40	5.232108859126768
120	41	-4.137553087397492
120	42	-1.7825914573595831
120	43	-5.814112747861817
120	44	-13.76327538781853
120	45	-3.434966523303153
120	46	-7.630709372535076
120	47	-7.474843181075428
120	48	-6.02368485868916
120	49	-13.186540093050837
120	50	-5.3381014375160625
120	51	-2.0380933397492775
120	52	-3.6090788288688564
120	53	-1.4687020686920356
120	54	-1.3607039529165696
120	55	-1.0650567459688745
120	56	0.07391500201804391
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	615.0
5	52.0
6	21.0
7	53.0
8	47.0
9	51.0
10	44.0
11	47.0
12	63.0
13	54.0
14	78.0
15	75.0
16	99.0
17	130.0
18	166.0
19	235.0
20	278.0
21	387.0
22	418.0
23	544.0
24	585.0
25	603.0
26	690.0
27	703.0
28	798.0
29	822.0
30	854.0
31	960.0
32	1091.0
33	1236.0
34	1343.0
35	1413.0
36	1573.0
37	1559.0
38	1461.0
39	1125.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	100.0	0.0	0.0	0.0
3	0.0	0.0	0.0	100.0
4	100.0	0.0	0.0	0.0
5	100.0	0.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	99.48206974794061	0.4094115325802792	0.06412469787401964	0.04439402160509051
8	99.55112711488187	0.28116213683223995	0.0986533813446456	0.0690573669412519
9	98.02693237310709	0.6609776550091254	0.9322744537069008	0.3798155181768855
10	49.96793765106299	21.368322399250232	21.698811226754795	6.964928722931979
11	30.676268929117544	20.42618260740887	17.520840526809057	31.376707936664527
12	28.367779805652837	26.75479702066788	19.015439254180436	25.861983919498844
13	21.042766240812906	15.429388842302568	22.961574507966258	40.566270408918264
14	22.473240270310267	32.48655847679179	22.74453706900804	22.2956641838899
15	36.36769299419902	18.037582428479347	21.156229857702414	24.438494719619218
16	28.194245669446776	31.83141686818339	20.584316241425256	19.39002122094458
17	28.073810933491217	18.39846062759029	35.14900335504243	18.37872508387606
18	24.45617323533764	18.699748433877573	20.746806096778965	36.09727223400582
19	25.878231695283205	18.43299782909019	20.80619696072627	34.882573514900336
20	25.295076300064203	20.24791347720875	34.618993530544714	19.838016692182332
21	25.097420214077836	18.50244167118828	23.444976076555022	32.95516203817886
22	22.271000838553743	18.00917476446505	37.97168647955409	21.748137917427123
23	22.07369407586445	20.702412075173875	35.431361909929464	21.792531939032212
24	21.976124704025256	21.527229676400946	22.849250197316493	33.6473954222573
25	24.231509350175163	21.971678097399714	33.260966102531206	20.535846449893917
26	38.29829236995361	22.62363044122002	18.892508143322477	20.185569045503897
27	23.615635179153095	34.02921725397295	18.38416740696871	23.970980159905242
28	28.84767166535122	29.898382004735595	19.430741910023677	21.823204419889503
29	32.43109750074088	28.60812012249333	19.529783660970068	19.430998715795713
30	29.576074618763265	21.521985885604302	28.7864580762967	20.115481419335733
31	32.00631443934685	28.21765083123674	20.778451975728874	18.997582753687535
32	32.629397542803574	27.098238515813883	21.52267232446835	18.749691616914195
33	32.63391755122192	26.70945445568995	20.794865465317205	19.86176252777092
34	23.72789250074103	21.81108586108092	36.859006027072425	17.602015611105625
35	22.22990321943512	23.32115346632431	36.450720916452696	17.99822239778787
36	27.65495459928938	23.44551914725622	29.14528227398342	19.754243979470985
37	31.149079057824302	21.179201027109773	28.77882573700064	18.89289417806528
38	32.12133188420117	22.897934986661397	26.07449856733524	18.906234561802194
39	30.502025891886554	23.337286293111966	20.06621207629212	26.09447573870936
40	25.380256486728303	18.297047420220697	25.66358484938861	30.65911124366239
41	21.05132037867464	16.731439960139515	23.95615346287992	38.261086198305925
42	21.765781798408227	27.91042562657571	22.388649957981116	27.93514261703495
43	17.81703033001831	26.757706199594278	28.03423878086191	27.391024689525505
44	20.156613966397384	27.060514447142786	27.660207166575805	25.12266441988403
45	28.028656126482215	21.516798418972332	29.11067193675889	21.34387351778656
46	20.252475247524753	27.93564356435644	24.0	27.811881188118814
47	18.44872049196588	24.7768299940488	22.84268994247173	33.93175957151359
48	26.549153211869847	24.006320051350414	24.248259517108576	25.19626721967116
49	20.08707698396992	26.335840094993074	22.625173164456758	30.95190975658025
50	20.9171319859663	28.62084301032762	24.37614270889954	26.085882294806545
51	20.597694324872595	27.697788333085942	24.86269853050319	26.84181881153827
52	21.58035184819134	33.02035975489227	25.1433089543388	20.255979442577583
53	19.364090392127775	33.254215497206154	26.726994016713647	20.65470009395243
54	20.622433328385533	23.838503785067537	29.90450744644006	25.634555440106872
55	20.236446379105658	22.858132172536607	30.797388207360505	26.10803324099723
56	19.69255103553952	23.528248727200832	31.827393603875244	24.95180663338441
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	1.0
8	2.0
9	3.0
10	2.5
11	2.0
12	7.5
13	20.0
14	27.0
15	38.0
16	49.0
17	50.5
18	52.0
19	49.5
20	47.0
21	47.0
22	49.5
23	52.0
24	56.0
25	60.0
26	70.0
27	80.0
28	91.0
29	96.5
30	91.0
31	116.5
32	142.0
33	146.0
34	150.0
35	163.0
36	176.0
37	210.0
38	273.0
39	302.0
40	303.0
41	304.0
42	368.0
43	432.0
44	505.5
45	579.0
46	740.5
47	1246.5
48	1591.0
49	1686.5
50	1782.0
51	1549.5
52	1317.0
53	1256.0
54	1211.0
55	1227.0
56	1219.5
57	1212.0
58	1239.5
59	1267.0
60	1274.0
61	1281.0
62	1286.0
63	1255.0
64	1219.0
65	1039.0
66	859.0
67	789.0
68	719.0
69	595.0
70	471.0
71	423.5
72	296.0
73	216.0
74	188.0
75	160.0
76	124.0
77	88.0
78	71.0
79	48.0
80	42.0
81	42.0
82	42.0
83	23.5
84	5.0
85	2.5
86	0.0
87	1.0
88	1.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.5129975829921571
16	0.0493266906723228
17	0.0246633453361614
18	0.0
19	0.0246633453361614
20	0.1183840576135747
21	0.0
22	0.0
23	0.0
24	0.004932669067232279
25	0.029596014403393676
26	0.05425935973955508
27	0.05425935973955508
28	0.004932669067232279
29	0.13318206481527153
30	0.0493266906723228
31	0.009865338134464559
32	0.029596014403393676
33	0.08878804321018102
34	0.15291274108420067
35	0.10358605041187786
36	0.04439402160509051
37	0.10851871947911015
38	0.15291274108420067
39	0.1726434173531298
40	0.7645637054210034
41	1.0013318206481527
42	0.2170374389582203
43	0.30582548216840133
44	0.4735362304542988
45	0.16277807921866522
46	0.36008484190795637
47	0.5376609283283185
48	0.0986533813446456
49	0.30089281310116905
50	0.17757608642036204
51	0.30582548216840133
52	0.18250875548759432
53	0.24663345336161396
54	0.30582548216840133
55	0.28116213683223995
56	0.20717210082375573
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
56	20273.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.98549795294234
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.86084756483238	70.078429438169
2	6.2745098039215685	9.786415429388843
3	1.6951296647691336	3.9658659300547523
4	0.6957621758380772	2.1703743895822027
5	0.41745730550284627	1.6277807921866523
6	0.3225806451612903	1.5093967345730774
7	0.11385199240986717	0.6215163024712672
8	0.1454775458570525	0.9076111083707394
9	0.050600885515496526	0.3551521728407241
>10	0.39215686274509803	4.8685443693582595
>50	0.012650221378874131	0.6363143096729641
>100	0.018975332068311195	3.472599023331525
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TGCGGAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT	329	1.6228481231194198	No Hit
TGCGGAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC	272	1.34168598628718	No Hit
TGCGGAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC	103	0.5080649139249248	No Hit
TGCGGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	74	0.36501751097518864	No Hit
TGCGGAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT	55	0.27129679869777534	No Hit
TGCGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	50	0.24663345336161396	No Hit
TGCGGAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA	50	0.24663345336161396	No Hit
TGCGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	39	0.19237409362205887	No Hit
TGCGGAGGGTCACAGATCCTCTGAAGGAGATTGGGCGGATTGAACAAAAACTCATC	27	0.13318206481527153	No Hit
TGCGGAGGGAGCCTGCCATCCCATTTGGATGTATCAATGCCACTGAGTTGTCTGTC	27	0.13318206481527153	No Hit
TGCGGAGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT	25	0.12331672668080698	No Hit
TGCGGAGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA	24	0.1183840576135747	No Hit
TGCGGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG	24	0.1183840576135747	No Hit
TGCGGAGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT	24	0.1183840576135747	No Hit
TGCGGAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGACCAAAAACTC	23	0.11345138854634243	No Hit
TGCGGAGGGTCACAGATCCTCTCCTGAAGGAGATTGGGCGGATTGAACAAAAACTC	22	0.10851871947911015	No Hit
TGCGGAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCTAATCCGCCCAATCT	22	0.10851871947911015	No Hit
TGCGGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT	21	0.10358605041187786	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.009865338134464559	0.0	0.0	0.0	0.0
11	0.014798007201696838	0.0	0.0	0.0	0.0
12	0.014798007201696838	0.0	0.0	0.0	0.0
13	0.014798007201696838	0.0	0.0	0.0	0.0
14	0.05919202880678735	0.0	0.0	0.0	0.0
15	0.05919202880678735	0.0	0.0	0.0	0.0
16	0.06412469787401963	0.0	0.0	0.0	0.0
17	0.0690573669412519	0.0	0.0	0.0	0.0
18	0.0690573669412519	0.0	0.0	0.0	0.0
19	0.0739900360084842	0.0	0.0	0.0	0.0
20	0.0739900360084842	0.0	0.0	0.0	0.0
21	0.07892270507571647	0.0	0.0	0.0	0.0
22	0.07892270507571647	0.0	0.0	0.0	0.0
23	0.07892270507571647	0.0	0.0	0.0	0.0
24	0.08385537414294875	0.0	0.0	0.0	0.0
25	0.08878804321018102	0.0	0.0	0.0	0.0
26	0.09372071227741331	0.0	0.0	0.0	0.0
27	0.10358605041187786	0.0	0.0	0.0	0.0
28	0.11345138854634243	0.0	0.0	0.0	0.0
29	0.12331672668080698	0.0	0.0	0.0	0.0
30	0.12824939574803926	0.0	0.0	0.0	0.0
31	0.15291274108420067	0.0	0.0	0.0	0.0
32	0.15784541015143294	0.0	0.0	0.0	0.0
33	0.15784541015143294	0.0	0.0	0.0	0.0
34	0.15784541015143294	0.0	0.0	0.0	0.0
35	0.15784541015143294	0.0	0.0	0.0	0.0
36	0.15784541015143294	0.0	0.0	0.0	0.0
37	0.16277807921866522	0.0	0.0	0.0	0.0
38	0.16277807921866522	0.0	0.0	0.0	0.0
39	0.17264341735312977	0.0	0.0	0.0	0.0
40	0.17264341735312977	0.0	0.0	0.0	0.0
41	0.17264341735312977	0.0	0.0	0.0	0.0
42	0.18250875548759435	0.0	0.0	0.0	0.0
43	0.20223943175652345	0.0	0.0	0.0	0.0
44	0.22197010802545258	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATTGAA	20	3.8286168E-4	50.65995	41
AAAGATT	20	3.8286168E-4	50.65995	47
GACACAA	15	0.007890476	50.659946	45
ACACAAA	15	0.007890476	50.659946	46
TAATCCG	15	0.007969633	50.53266	42
CATCCGC	15	0.008049379	50.406013	43
GCGCATT	20	4.0237757E-4	50.154617	38
CGCATTG	20	4.0237757E-4	50.154617	39
GCATTGA	20	4.0237757E-4	50.154617	40
CAACAAA	15	0.008210652	50.154613	40
ACAACAA	15	0.008210652	50.154613	39
TTTTGTC	30	9.928517E-7	50.02985	35
TTGTCTA	15	0.008292182	50.02985	37
TGGTTGA	25	2.007398E-5	50.02985	14
GGTTGAT	25	2.007398E-5	50.02985	15
GCCCAAG	15	0.008292182	50.02985	48
GGCGCAT	20	4.073779E-4	50.02985	37
TAGCATA	20	4.073779E-4	50.02985	30
AAGATTA	20	4.073779E-4	50.02985	48
AGCATAT	20	4.073779E-4	50.02985	31
>>END_MODULE