FastQCFastQC Report
Fri 17 Jun 2016
SRR1525933_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525933_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3246
Sequences flagged as poor quality0
Sequence length40
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCGGAGGGATGAGATGGACTTAAAGGAATTGACGGGTAC411.2630930375847198No Hit
TGCGGAGGGATGAGATGGACTTAAATGAATTGACGGGTAC351.0782501540357363No Hit
TGCGGAGGGATGAGATGGCCTTAAAGGAATTGACGGGTAC240.7393715341959335No Hit
TGCGGAGGGATGAGATGGACTCAAAGGAATTGACGGGTAC210.6469500924214419No Hit
TGCGGAGGGATGAGATGGCCTTAAATGAATTGACGGGTAC190.5853357979051139No Hit
TGCGGAGGGATGAGATGGACTCAAATGAATTGACGGGTAC140.43130006161429446No Hit
TGCGGAGGGATGAGATGGCCTCAAAGGAATTGACGGGTAC120.36968576709796674No Hit
TGCGGAGGGATGAGATGGCCTTAAAGGAATTGACCGGTAC70.21565003080714723No Hit
TGCGGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT70.21565003080714723No Hit
TGCGGAGGGATGAGATGGACTTACAGGAATTGACGGGTAC60.18484288354898337No Hit
TGCGGAGGGATGAGATGGCCTCAAATGAATTGACGGGTAC60.18484288354898337No Hit
TGCGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC60.18484288354898337No Hit
TGCGGAGGGCTCGTCTTAATCAGCAACATCTTTTGATACA50.15403573629081946No Hit
TGCGGAGGGATGAGCTGGACTTAAAGGAATTGACGGGTAC50.15403573629081946No Hit
TGCGGAGGGATGAGATGGACTCAAATGAATTGACCGGTAC50.15403573629081946No Hit
TGCGGAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT50.15403573629081946No Hit
TGCGGAGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT50.15403573629081946No Hit
TGCGGAGGGATGAGATGGCCTTCAATGAATTGACGGGTAC50.15403573629081946No Hit
TGCGGAGGGATGAGATGGCCTTACATGAATTGACGGGTAC50.15403573629081946No Hit
TGCGGAGGGATGAGATGGCCTCCAAGGAATTGACGGGTAC40.12322858903265559No Hit
TGCGGAGGGTCAGTCGATTTTTTTTTTTTTTTTTTTTTTT40.12322858903265559No Hit
TGCGGAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.12322858903265559No Hit
TGCGGAGGGATGAGATGGCCTCAAATGAATTGACCGGTAC40.12322858903265559No Hit
TGCGGAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.12322858903265559No Hit
TGCGGAGGGATGAGATGGACTTAAAGGACTTGACGGGTAC40.12322858903265559No Hit
TGCGGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.12322858903265559No Hit
TGCGGAGGGTGCGGAGGGATGAGATGGACTTAAATGAATT40.12322858903265559No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG301.0989528E-533.8906256
GGACTCA301.0989528E-533.89062517
ACTCAAA251.6437449E-433.89062519
TGGACTC301.0989528E-533.89062516
GGAGGGT200.00243375233.8906254
GGAGGGC200.00243375233.8906254
GAGGGGT200.00243375233.8906255
GAGGGGG501.9463187E-1033.8906255
GAGGGGA453.0613592E-933.8906255
GAGGGAG200.00243375233.8906255
GACTCAA251.6437449E-433.89062518
GGAGGGG1400.032.6802444
TGCGGAG3500.030.9857121
GCGGAGG3450.030.9436132
CGGAGGG3350.030.8556443
GAGGGAA353.159495E-529.0491075
GGAGGGA1550.028.4243954
CGGGTAC304.7970266E-428.24218834
GGATGAG503.3795004E-727.11258
GACGGGT250.00726095127.112532
Produced by FastQC (version 0.11.5)