##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1525933_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3246 Sequences flagged as poor quality 0 Sequence length 40 %GC 51 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.610289587184226 35.0 31.0 38.0 12.0 38.0 2 26.924214417744917 32.0 20.0 36.0 4.0 38.0 3 25.03203943314849 29.0 17.0 33.0 4.0 36.0 4 26.15403573629082 31.0 17.0 36.0 4.0 38.0 5 26.690388170055453 32.0 18.0 36.0 4.0 38.0 6 24.621072088724585 29.0 16.0 35.0 4.0 38.0 7 28.092729513247072 33.0 25.0 36.0 4.0 38.0 8 27.040357362908196 32.0 22.0 36.0 4.0 38.0 9 25.016019716574245 29.0 22.0 32.0 4.0 35.0 10 20.44947627849661 22.0 10.0 30.0 4.0 35.0 11 24.49260628465804 29.0 15.0 34.0 4.0 36.0 12 24.497535428219347 29.0 15.0 35.0 4.0 37.0 13 24.773567467652494 29.0 18.0 34.0 4.0 37.0 14 25.8120764017252 31.0 19.0 35.0 4.0 38.0 15 22.694701170671596 27.0 11.0 33.0 4.0 36.0 16 21.976894639556377 26.0 4.0 33.0 4.0 36.0 17 22.40973505853358 27.0 4.0 33.0 4.0 36.0 18 22.01139864448552 26.0 4.0 33.0 4.0 36.0 19 18.717498459642638 19.0 4.0 30.0 4.0 34.0 20 21.092729513247072 26.0 4.0 33.0 4.0 36.0 21 18.149414664202094 19.0 4.0 30.0 4.0 35.0 22 18.261860751694392 19.0 4.0 31.0 4.0 35.0 23 17.54282193468885 18.0 4.0 30.0 4.0 35.0 24 17.647874306839185 18.0 4.0 30.0 4.0 35.0 25 16.955945779420826 16.0 4.0 30.0 4.0 35.0 26 15.407578558225508 4.0 4.0 29.0 4.0 35.0 27 14.97935921133703 4.0 4.0 29.0 4.0 35.0 28 14.104744300677757 4.0 4.0 27.0 4.0 33.0 29 13.876463339494762 4.0 4.0 27.0 4.0 33.0 30 12.862600123228589 4.0 4.0 24.0 4.0 32.0 31 12.448243992606285 4.0 4.0 25.0 4.0 33.0 32 11.528650646950092 4.0 4.0 22.0 4.0 31.0 33 11.143561306223043 4.0 4.0 20.0 4.0 31.0 34 11.377387553912508 4.0 4.0 22.0 4.0 32.0 35 10.373690696241528 4.0 4.0 16.0 4.0 30.0 36 10.632162661737523 4.0 4.0 16.0 4.0 32.0 37 10.329944547134936 4.0 4.0 14.0 4.0 32.0 38 10.492606284658041 4.0 4.0 15.0 4.0 32.0 39 9.916820702402957 4.0 4.0 10.0 4.0 31.0 40 10.222427603203943 4.0 4.0 4.0 4.0 32.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -21.819570538495768 1 2 -22.525634178905207 1 3 -20.53812861593235 1 4 -21.757476635514006 1 5 -22.107988429016473 1 6 -19.600155763239876 1 7 -23.23031820204717 1 8 -21.686203827325322 1 9 -20.268268802848244 1 10 -15.76985981308411 1 11 -18.997018246550958 1 12 -18.599955496217177 1 13 -19.84164441477526 1 14 -20.428282153983087 1 15 -17.579684023141972 1 16 -16.26515353805073 1 17 -17.261415220293728 1 18 -16.408566978193146 1 19 -13.33872941700044 1 20 -16.40427236315087 1 21 -13.775433911882505 1 22 -13.795004450378283 1 23 -13.158700489541612 1 24 -13.447841566533157 1 25 -13.18896306186026 1 26 -11.914085447263018 1 27 -11.533233199821984 1 28 -10.404327992879395 1 29 -10.608867378727194 1 30 -9.686381842456612 1 31 -8.904862038273253 1 32 -7.480574098798396 1 33 -8.116633288829549 1 34 -7.914185580774367 1 35 -6.215520694259013 1 36 -6.108911882510016 1 37 -5.786159323542503 1 38 -6.1064975522919465 1 39 -6.022107254116602 1 40 -5.978526924788607 2 1 -8.019570538495767 2 2 -4.525634178905207 2 3 -13.53812861593235 2 4 -10.757476635514006 2 5 -2.1079884290164728 2 6 -18.600155763239876 2 7 -3.230318202047169 2 8 1.313796172674678 2 9 -0.26826880284824384 2 10 -1.7698598130841106 2 11 2.002981753449042 2 12 -12.599955496217177 2 13 -14.84164441477526 2 14 0.571717846016913 2 15 -14.579684023141972 2 16 -0.26515353805072905 2 17 0.7385847797062723 2 18 1.5914330218068535 2 19 -4.33872941700044 2 20 3.59572763684913 2 21 15.224566088117495 2 22 13.204995549621717 2 23 13.841299510458388 2 24 -7.447841566533157 2 25 10.81103693813974 2 26 -5.914085447263018 2 27 12.466766800178016 2 28 10.595672007120605 2 29 8.391132621272806 2 30 7.313618157543388 2 31 14.095137961726747 2 32 15.519425901201604 2 33 20.883366711170453 2 34 -0.9141855807743671 2 35 19.784479305740987 2 36 -6.108911882510016 2 37 -5.786159323542503 2 38 -6.1064975522919465 2 39 -6.022107254116602 2 40 -5.978526924788607 5 1 -6.019570538495767 5 2 -10.525634178905207 5 3 0.21187138406764916 5 4 -15.507476635514006 5 5 -8.107988429016473 5 6 -10.350155763239876 5 7 -4.730318202047169 5 8 -7.936203827325322 5 9 -14.268268802848244 5 10 -13.01985981308411 5 11 -8.247018246550958 5 12 -0.34995549621717714 5 13 -4.34164441477526 5 14 -1.178282153983087 5 15 -9.329684023141972 5 16 1.734846461949271 5 17 3.4885847797062723 5 18 3.5914330218068535 5 19 3.9112705829995598 5 20 3.09572763684913 5 21 0.2245660881174949 5 22 7.204995549621717 5 23 9.591299510458388 5 24 9.052158433466843 5 25 2.0610369381397398 5 26 4.835914552736982 5 27 2.216766800178016 5 28 5.345672007120605 5 29 3.6411326212728063 5 30 -3.686381842456612 5 31 5.595137961726747 5 32 -1.480574098798396 5 33 -1.366633288829549 5 34 -1.914185580774367 5 35 0.5344793057409873 5 36 0.6410881174899838 5 37 0.9638406764574974 5 38 -1.1064975522919465 5 39 -0.022107254116601638 5 40 0.771473075211393 6 1 3.1470961281708973 6 2 3.1410324877614606 6 3 5.128538050734317 6 4 -2.7574766355140063 6 5 3.8920115709835272 6 6 5.399844236760124 6 7 2.6030151312861634 6 8 3.480462839341346 6 9 2.5650645304850883 6 10 2.7301401869158894 6 11 -2.163684913217626 6 12 4.400044503782823 6 13 -0.6749777481085921 6 14 1.571717846016913 6 15 8.586982643524696 6 16 -4.265153538050729 6 17 -2.09474855362706 6 18 0.4247663551401857 6 19 5.827937249666228 6 20 2.09572763684913 6 21 7.057899421450827 6 22 3.871662216288385 6 23 5.67463284379172 6 24 5.885491766800175 6 25 10.644370271473072 6 26 7.75258121940365 6 27 6.633433466844684 6 28 11.762338673787273 6 29 8.557799287939474 6 30 5.980284824210056 6 31 8.42847129506008 6 32 6.519425901201604 6 33 7.383366711170451 6 34 3.585814419225633 6 35 -0.38218736092567873 6 36 -3.608911882510016 6 37 -2.7861593235425026 6 38 -2.1064975522919465 6 39 -1.8554405874499356 6 40 -1.311860258121941 7 1 5.980429461504233 7 2 10.807699154428128 7 3 7.461871384067649 7 4 8.409190031152658 7 5 10.725344904316863 7 6 11.899844236760124 7 7 6.603015131286167 7 8 5.480462839341346 7 9 5.565064530485088 7 10 9.896806853582557 7 11 7.836315086782374 7 12 4.733377837116155 7 13 10.15835558522474 7 14 3.238384512683581 7 15 10.086982643524696 7 16 1.568179795282603 7 17 6.40525144637294 7 18 2.9247663551401857 7 19 6.494603916332892 7 20 5.09572763684913 7 21 11.224566088117495 7 22 11.03832888295505 7 23 7.17463284379172 7 24 9.218825100133511 7 25 4.477703604806408 7 26 -1.0807521139296838 7 27 -1.033233199821984 7 28 -0.23766132621272895 7 29 -0.27553404539385973 7 30 1.146951490876722 7 31 -2.7381953716065865 7 32 -0.8139074321317299 7 33 -1.616633288829549 7 34 1.085814419225633 7 35 0.11781263907432127 7 36 -0.9422452158433501 7 37 -1.1194926568758365 7 38 -0.9398308856252804 7 39 1.9778927458833984 7 40 1.688139741878059 8 1 4.147096128170897 8 2 6.141032487761457 8 3 2.961871384067649 8 4 -0.0908099688473385 8 5 0.39201157098352724 8 6 6.566510903426792 8 7 3.6030151312861634 8 8 5.813796172674678 8 9 2.5650645304850883 8 10 -4.7698598130841106 8 11 6.66964842011571 8 12 1.5667111704494907 8 13 2.825022251891408 8 14 -2.428282153983087 8 15 -7.413017356475306 8 16 -1.5984868713840612 8 17 -7.761415220293728 8 18 -5.9085669781931465 8 19 -3.0053960836671063 8 20 -3.237605696484202 8 21 -0.7754339118825051 8 22 -0.7950044503782827 8 23 5.67463284379172 8 24 3.8854917668001754 8 25 0.8110369381397398 8 26 -1.0807521139296838 8 27 -5.19989986648865 8 28 -3.070994659546061 8 29 -5.942200712060528 8 30 -2.353048509123278 8 31 -3.0715287049399187 8 32 -2.480574098798396 8 33 -0.11663328882954893 8 34 -2.080852247441033 8 35 1.4511459724076534 8 36 2.391088117489984 8 37 -0.7861593235425026 8 38 2.5601691143747196 8 39 -1.5221072541166016 8 40 -1.478526924788607 9 1 5.980429461504233 9 2 9.974365821094793 9 3 9.795204717400985 9 4 9.909190031152658 9 5 9.058678237650192 9 6 1.3998442367601243 9 7 9.103015131286167 9 8 10.980462839341342 9 9 9.231731197151756 9 10 10.73014018691589 9 11 0.6696484201157098 9 12 4.900044503782823 9 13 2.491688918558072 9 14 0.571717846016913 9 15 3.75364931019136 9 16 5.234846461949271 9 17 6.238584779706272 9 18 11.258099688473521 9 19 1.3279372496662276 9 20 9.262394303515798 9 21 15.224566088117495 9 22 8.53832888295505 9 23 12.341299510458388 9 24 13.218825100133511 9 25 15.644370271473075 9 26 3.4192478860703144 9 27 6.633433466844684 9 28 0.26233867378727105 9 29 -0.4422007120605276 9 30 0.9802848242100541 9 31 -0.0715287049399187 9 32 2.852759234534938 9 33 -0.9499666221628829 9 34 4.085814419225633 9 35 -0.38218736092567873 9 36 1.8910881174899838 9 37 1.5471740097908313 9 38 2.0601691143747196 9 39 1.4778927458833984 9 40 1.688139741878059 10 1 1.605429461504233 10 2 2.9743658210947928 10 3 1.5868713840676492 10 4 4.492523364485994 10 5 5.392011570983527 10 6 8.399844236760124 10 7 4.894681797952831 10 8 6.813796172674678 10 9 5.731731197151756 10 10 0.35514018691588944 10 11 2.752981753449042 10 12 4.650044503782823 10 13 1.03335558522474 10 14 3.071717846016913 10 15 3.170315976858028 10 16 6.984846461949271 10 17 6.488584779706272 10 18 7.8414330218068535 10 19 8.78627058299956 10 20 5.59572763684913 10 21 6.474566088117495 10 22 5.829995549621717 10 23 8.591299510458388 10 24 6.052158433466843 10 25 6.06103693813974 10 26 6.835914552736982 10 27 4.841766800178016 10 28 5.220672007120605 10 29 4.266132621272806 10 30 5.563618157543388 10 31 4.720137961726747 10 32 -1.980574098798396 10 33 -3.241633288829549 10 34 -1.539185580774367 10 35 -0.09052069425901266 10 36 -1.2339118825100162 10 37 1.9638406764574974 10 38 -0.8564975522919465 10 39 1.3528927458833984 10 40 -0.728526924788607 11 1 2.5804294615042345 11 2 2.674365821094792 11 3 2.461871384067649 11 4 3.2425233644859937 11 5 2.8920115709835272 11 6 4.999844236760126 11 7 0.3696817979528326 11 8 4.513796172674677 11 9 1.3317311971517576 11 10 5.430140186915889 11 11 3.4029817534490405 11 12 -6.399955496217178 11 13 2.5583555852247386 11 14 0.9717178460169116 11 15 0.8203159768580264 11 16 3.734846461949271 11 17 4.938584779706272 11 18 4.391433021806854 11 19 8.46127058299956 11 20 4.395727636849131 11 21 7.424566088117494 11 22 10.804995549621719 11 23 11.641299510458389 11 24 6.952158433466842 11 25 11.61103693813974 11 26 10.885914552736983 11 27 8.066766800178018 11 28 9.395672007120606 11 29 6.191132621272807 11 30 11.313618157543388 11 31 9.295137961726747 11 32 13.919425901201603 11 33 12.483366711170452 11 34 12.085814419225633 11 35 7.184479305740986 11 36 7.891088117489984 11 37 7.213840676457497 11 38 2.6935024477080542 11 39 8.5778927458834 11 40 3.4214730752113933 12 1 4.837572318647087 12 2 7.7600801068090774 12 3 2.033299955496222 12 4 7.956809078771709 12 5 10.034868713840673 12 6 11.11412995104584 12 7 7.4839675122385465 12 8 10.599510458388963 12 9 9.01744548286604 12 10 12.087283044058747 12 11 14.288696039163327 12 12 12.542901646639969 12 13 12.58692701379617 12 14 10.286003560302628 12 15 14.277458834000882 12 16 13.449132176234986 12 17 14.738584779706272 12 18 12.448575878949708 12 19 9.946984868713844 12 20 11.452870493991988 12 21 17.653137516688926 12 22 16.490709835336002 12 23 16.984156653315534 12 24 17.980729862038274 12 25 17.81103693813974 12 26 17.51448598130841 12 27 16.60962394303516 12 28 18.595672007120605 12 29 18.391132621272806 12 30 18.885046728971957 12 31 4.809423676012463 12 32 3.6622830440587464 12 33 6.740509568313309 12 34 7.085814419225633 12 35 3.498765020026701 12 36 6.1768024032042685 12 37 7.642412105028924 12 38 4.322073876279482 12 39 6.977892745883398 12 40 7.45004450378282 13 1 -7.733856252781482 13 2 -16.525634178905207 13 3 -14.53812861593235 13 4 -15.900333778371149 13 5 -15.536559857587902 13 6 -14.31444147752559 13 7 -13.658889630618598 13 8 -12.829060970182464 13 9 -12.268268802848244 13 10 -11.341288384512682 13 11 -10.854161103693816 13 12 -8.885669781931464 13 13 -10.698787271918118 13 14 -9.57113929684023 13 15 -8.43682688028483 13 16 -4.693724966622158 13 17 -5.6899866488651565 13 18 -4.551424121050289 13 19 -6.910157988429011 13 20 -4.547129506008012 13 21 -6.346862483311076 13 22 -5.795004450378283 13 23 -5.444414775255899 13 24 -5.304984423676014 13 25 -4.903248776145974 13 26 -8.056942590120162 13 27 -8.104661771250555 13 28 -8.975756564307966 13 29 -6.751724521584337 13 30 -7.114953271028041 13 31 -5.476290609701824 13 32 -6.19485981308411 13 33 -5.68806186025812 13 34 -4.485614152202938 13 35 -3.358377837116156 13 36 -2.966054739652873 13 37 -3.6433021806853594 13 38 -2.6779261237205176 13 39 -5.307821539830887 13 40 -2.2642412105028926 14 1 0.48042946150423305 14 2 1.4743658210947928 14 3 -5.538128615932351 14 4 -0.7574766355140063 14 5 0.39201157098352724 14 6 1.8998442367601243 14 7 6.269681797952831 14 8 7.313796172674678 14 9 4.231731197151756 14 10 4.2301401869158894 14 11 3.002981753449042 14 12 7.400044503782823 14 13 7.65835558522474 14 14 3.071717846016913 14 15 5.420315976858028 14 16 7.234846461949271 14 17 15.238584779706272 14 18 16.591433021806854 14 19 19.66127058299956 14 20 16.09572763684913 14 21 18.724566088117495 14 22 17.204995549621717 14 23 4.841299510458388 14 24 4.552158433466843 14 25 4.81103693813974 14 26 6.085914552736982 14 27 6.466766800178016 14 28 5.095672007120605 14 29 7.391132621272806 14 30 8.313618157543388 14 31 4.595137961726747 14 32 5.519425901201604 14 33 5.383366711170451 14 34 5.585814419225633 14 35 7.284479305740987 14 36 -6.108911882510016 14 37 -5.786159323542503 14 38 -6.1064975522919465 14 39 -6.022107254116602 14 40 -5.978526924788607 15 1 6.647096128170897 15 2 10.474365821094793 15 3 12.628538050734313 15 4 10.07585669781933 15 5 10.058678237650192 15 6 9.399844236760124 15 7 9.269681797952831 15 8 11.147129506008014 15 9 8.565064530485092 15 10 8.896806853582557 15 11 11.002981753449042 15 12 2.0667111704494907 15 13 8.491688918558076 15 14 10.905051179350249 15 15 12.753649310191363 15 16 9.568179795282603 15 17 12.405251446372937 15 18 8.758099688473521 15 19 11.66127058299956 15 20 11.929060970182466 15 21 12.224566088117495 15 22 11.871662216288385 15 23 13.17463284379172 15 24 15.718825100133508 15 25 9.31103693813974 15 26 11.25258121940365 15 27 8.633433466844684 15 28 7.429005340453937 15 29 4.7244659546061385 15 30 -0.853048509123278 15 31 1.7618046283934135 15 32 3.519425901201604 15 33 2.050033377837117 15 34 4.919147752558965 15 35 -2.5488540275923457 15 36 -0.10891188251001616 15 37 0.047174009790831306 15 38 -2.6064975522919465 15 39 0.8112260792167323 15 40 3.021473075211393 16 1 5.480429461504233 16 2 9.641032487761457 16 3 7.128538050734317 16 4 5.075856697819326 16 5 10.392011570983527 16 6 1.5665109034267921 16 7 6.769681797952831 16 8 9.813796172674678 16 9 5.898397863818424 16 10 3.0634735202492216 16 11 9.836315086782378 16 12 5.733377837116155 16 13 5.15835558522474 16 14 8.738384512683577 16 15 7.25364931019136 16 16 6.234846461949271 16 17 10.071918113039604 16 18 12.42476635514019 16 19 4.66127058299956 16 20 8.59572763684913 16 21 8.057899421450827 16 22 10.53832888295505 16 23 7.841299510458388 16 24 14.052158433466843 16 25 10.644370271473072 16 26 15.085914552736982 16 27 14.800100133511348 16 28 11.262338673787273 16 29 12.224465954606138 16 30 16.813618157543388 16 31 4.9284712950600795 16 32 5.519425901201604 16 33 5.550033377837119 16 34 7.419147752558965 16 35 2.1178126390743213 16 36 2.891088117489984 16 37 0.21384067645749738 16 38 2.0601691143747196 16 39 -3.5221072541166016 16 40 -1.478526924788607 17 1 5.647096128170897 17 2 6.974365821094793 17 3 6.461871384067649 17 4 6.242523364485994 17 5 7.0586782376501915 17 6 9.899844236760124 17 7 8.603015131286167 17 8 8.813796172674678 17 9 8.39839786381842 17 10 6.896806853582557 17 11 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3.5859145527369822 108 27 2.966766800178016 108 28 5.095672007120605 108 29 2.8911326212728063 108 30 4.813618157543388 108 31 0.09513796172674738 108 32 -4.480574098798396 108 33 -0.6166332888295489 108 34 2.085814419225633 108 35 -0.21552069425901266 108 36 2.391088117489984 108 37 1.2138406764574974 108 38 -1.1064975522919465 108 39 0.9778927458833984 108 40 7.021473075211393 109 1 3.180429461504236 109 2 3.674365821094792 109 3 5.861871384067648 109 4 4.442523364485993 109 5 3.692011570983528 109 6 0.5998442367601236 109 7 4.969681797952834 109 8 0.9137961726746795 109 9 5.531731197151757 109 10 -0.16985981308410913 109 11 3.2029817534490412 109 12 1.2000445037828236 109 13 0.9583555852247407 109 14 5.171717846016914 109 15 -0.5796840231419722 109 16 6.334846461949272 109 17 6.538584779706273 109 18 5.991433021806852 109 19 9.46127058299956 109 20 3.59572763684913 109 21 -2.1754339118825055 109 22 0.2049955496217173 109 23 -4.3587004895416115 109 24 -0.44784156653315677 109 25 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110 24 -13.447841566533157 110 25 -13.18896306186026 110 26 -11.914085447263018 110 27 -11.533233199821984 110 28 -10.404327992879395 110 29 -10.608867378727194 110 30 -9.686381842456612 110 31 -8.904862038273253 110 32 -7.480574098798396 110 33 -8.116633288829549 110 34 -7.914185580774367 110 35 -6.215520694259013 110 36 -6.108911882510016 110 37 -5.786159323542503 110 38 -6.1064975522919465 110 39 -6.022107254116602 110 40 -5.978526924788607 111 1 5.313762794837569 111 2 9.807699154428128 111 3 11.128538050734313 111 4 8.242523364485994 111 5 10.225344904316863 111 6 -1.6001557632398757 111 7 6.769681797952831 111 8 -5.35287049399199 111 9 5.731731197151756 111 10 -4.436526479750777 111 11 -0.997018246550958 111 12 5.400044503782823 111 13 -10.508311081441926 111 14 -6.761615487316419 111 15 -10.579684023141972 111 16 -2.598486871384061 111 17 0.4052514463729402 111 18 0.5914330218068535 111 19 -2.0053960836671063 111 20 -0.07093902981753786 111 21 2.224566088117495 111 22 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0.59572763684913 112 21 1.724566088117495 112 22 1.7049955496217173 112 23 -0.6587004895416122 112 24 2.0521584334668432 112 25 2.3110369381397398 112 26 -7.414085447263018 112 27 -1.533233199821984 112 28 -5.904327992879395 112 29 -0.6088673787271937 112 30 -1.186381842456612 112 31 4.595137961726747 112 32 1.519425901201604 112 33 3.883366711170451 112 34 4.085814419225633 112 35 5.784479305740987 112 36 7.891088117489984 112 37 4.213840676457497 112 38 3.8935024477080535 112 39 7.977892745883398 112 40 4.021473075211393 114 1 -2.019570538495767 114 2 2.4743658210947928 114 3 -15.03812861593235 114 4 -1.7574766355140063 114 5 -2.6079884290164728 114 6 -3.1001557632398757 114 7 -6.730318202047169 114 8 4.313796172674678 114 9 -13.268268802848244 114 10 -2.2698598130841106 114 11 10.002981753449042 114 12 -3.599955496217177 114 13 3.15835558522474 114 14 9.071717846016913 114 15 1.9203159768580278 114 16 6.234846461949271 114 17 -7.261415220293728 114 18 -5.4085669781931465 114 19 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115 18 10.591433021806854 115 19 7.66127058299956 115 20 10.59572763684913 115 21 13.224566088117495 115 22 13.204995549621717 115 23 13.841299510458388 115 24 5.552158433466843 115 25 12.81103693813974 115 26 6.085914552736982 115 27 7.466766800178016 115 28 11.595672007120605 115 29 13.391132621272806 115 30 21.313618157543388 115 31 -8.904862038273253 115 32 -7.480574098798396 115 33 -8.116633288829549 115 34 -7.914185580774367 115 35 -6.215520694259013 115 36 -6.108911882510016 115 37 -5.786159323542503 115 38 -6.1064975522919465 115 39 -6.022107254116602 115 40 -5.978526924788607 118 1 0.980429461504233 118 2 2.4743658210947928 118 3 -14.53812861593235 118 4 2.2425233644859937 118 5 -0.10798842901647276 118 6 4.399844236760124 118 7 -17.23031820204717 118 8 2.313796172674678 118 9 -12.268268802848244 118 10 1.2301401869158894 118 11 12.002981753449042 118 12 6.400044503782823 118 13 9.15835558522474 118 14 4.571717846016913 118 15 13.420315976858028 118 16 -1.265153538050729 118 17 6.738584779706272 118 18 -7.4085669781931465 118 19 -7.33872941700044 118 20 -9.40427236315087 118 21 -7.775433911882505 118 22 -4.795004450378283 118 23 4.841299510458388 118 24 8.552158433466843 118 25 11.81103693813974 118 26 19.08591455273698 118 27 19.466766800178014 118 28 10.595672007120605 118 29 18.391132621272806 118 30 24.313618157543388 118 31 17.09513796172675 118 32 -7.480574098798396 118 33 -8.116633288829549 118 34 -7.914185580774367 118 35 -6.215520694259013 118 36 -6.108911882510016 118 37 -5.786159323542503 118 38 -6.1064975522919465 118 39 -6.022107254116602 118 40 -5.978526924788607 119 1 -27.019570538495767 119 2 -22.525634178905207 119 3 -20.53812861593235 119 4 -21.757476635514006 119 5 -22.107988429016473 119 6 -19.600155763239876 119 7 -23.23031820204717 119 8 -21.686203827325322 119 9 -20.268268802848244 119 10 -15.76985981308411 119 11 -18.997018246550958 119 12 -18.599955496217177 119 13 -19.84164441477526 119 14 -20.428282153983087 119 15 -17.579684023141972 119 16 -16.26515353805073 119 17 -17.261415220293728 119 18 -16.408566978193146 119 19 -13.33872941700044 119 20 -16.40427236315087 119 21 -13.775433911882505 119 22 -13.795004450378283 119 23 -13.158700489541612 119 24 -13.447841566533157 119 25 -13.18896306186026 119 26 -11.914085447263018 119 27 -11.533233199821984 119 28 -10.404327992879395 119 29 -10.608867378727194 119 30 -9.686381842456612 119 31 -8.904862038273253 119 32 -7.480574098798396 119 33 -8.116633288829549 119 34 -7.914185580774367 119 35 -6.215520694259013 119 36 -6.108911882510016 119 37 -5.786159323542503 119 38 -6.1064975522919465 119 39 -6.022107254116602 119 40 -5.978526924788607 >>END_MODULE >>Per sequence quality scores fail #Quality Count 4 436.0 5 21.0 6 8.0 7 20.0 8 17.0 9 37.0 10 57.0 11 61.0 12 75.0 13 92.0 14 106.0 15 113.0 16 135.0 17 135.0 18 152.0 19 127.0 20 143.0 21 157.0 22 163.0 23 174.0 24 128.0 25 160.0 26 163.0 27 140.0 28 120.0 29 105.0 30 81.0 31 63.0 32 30.0 33 13.0 34 13.0 35 0.0 36 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 100.0 0.0 0.0 0.0 3 0.0 0.0 0.0 100.0 4 100.0 0.0 0.0 0.0 5 100.0 0.0 0.0 0.0 6 0.0 100.0 0.0 0.0 7 99.07578558225508 0.7085643869377696 0.06161429451632779 0.15403573629081946 8 98.24345146379045 0.4930662557781202 0.4930662557781202 0.7704160246533128 9 97.74899784150477 1.5417823003391922 0.37002775208140615 0.33919210607462225 10 35.890326555760936 43.13000616142945 10.474430067775724 10.505237215033889 11 25.847196549599506 23.043746149106592 36.56808379544054 14.540973505853358 12 48.18237831176833 15.003080714725817 16.54343807763401 20.27110289587184 13 22.242760320394332 35.76709796672828 21.072088724584106 20.918052988293283 14 36.16759088108441 24.738139248305608 22.674060382008626 16.420209488601355 15 19.963031423290204 42.72951324707332 19.68576709796673 17.621688231669747 16 22.520024645717807 24.029574861367838 36.78373382624769 16.666666666666664 17 42.020948860135555 23.382624768946396 17.714109673444238 16.882316697473815 18 39.49476278496611 26.062846580406656 16.235366605052374 18.20702402957486 19 16.640938561284347 29.20654523000926 14.140166718122876 40.01234949058352 20 19.223659889094268 26.09365372766482 19.008009858287124 35.67467652495379 21 19.346888478126925 25.32347504621072 36.32162661737523 19.008009858287124 22 16.974738139248306 21.28773875539125 31.4849044978435 30.252618607516947 23 21.13370301910043 40.66543438077634 19.53173136167591 18.66913123844732 24 17.036352433764634 42.17498459642638 18.97720271102896 21.811460258780038 25 17.40203640851589 41.71551990126504 20.48750385683431 20.394939833384758 26 26.131432114073156 24.67451952882827 28.92126472411655 20.272783632982023 27 31.689272503082616 23.705302096177558 22.626387176325522 21.979038224414303 28 12.819722650231125 41.94144838212635 22.742681047765796 22.496147919876734 29 13.660191181005244 37.98951588035769 27.012025901942643 21.33826703669442 30 12.453760789149198 21.023427866831074 45.49938347718865 21.023427866831074 31 12.72390364422483 22.544780728844966 47.4366893143916 17.294626312538604 32 30.939907550077038 21.078582434514637 30.32357473035439 17.65793528505393 33 12.665639445300462 36.64098613251156 31.710323574730353 18.983050847457626 34 10.012322858903264 22.36598890942699 34.62723351817621 32.99445471349353 35 23.57473035439137 22.681047765793526 33.220338983050844 20.523882896764253 36 25.916795069337446 23.08166409861325 33.312788906009246 17.688751926040062 37 25.061728395061728 22.067901234567902 34.32098765432099 18.549382716049383 38 10.474430067775724 23.937153419593347 47.65865680837955 17.929759704251385 39 11.398644485520641 36.383240911891555 34.13431916204559 18.083795440542204 40 10.474430067775724 24.245224892174985 32.74799753542822 32.53234750462107 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 1.0 12 2.0 13 2.0 14 1.0 15 0.0 16 4.0 17 8.0 18 8.0 19 11.5 20 15.0 21 24.5 22 34.0 23 34.0 24 43.0 25 52.0 26 67.0 27 82.0 28 82.0 29 83.0 30 84.0 31 89.0 32 94.0 33 94.0 34 98.0 35 102.0 36 101.5 37 101.0 38 101.0 39 113.0 40 125.0 41 145.0 42 165.0 43 165.0 44 172.5 45 180.0 46 204.5 47 229.0 48 229.0 49 264.0 50 299.0 51 323.0 52 347.0 53 347.0 54 290.5 55 234.0 56 201.0 57 168.0 58 168.0 59 168.0 60 168.0 61 167.5 62 167.0 63 167.0 64 163.5 65 160.0 66 164.5 67 169.0 68 169.0 69 140.5 70 112.0 71 84.5 72 57.0 73 57.0 74 54.5 75 52.0 76 36.0 77 20.0 78 20.0 79 18.0 80 16.0 81 9.0 82 2.0 83 2.0 84 2.0 85 2.0 86 1.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.030807147258163897 9 0.09242144177449169 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.21565003080714723 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.15403573629081946 26 0.6161429451632778 27 0.06161429451632779 28 0.030807147258163897 29 0.09242144177449169 30 0.06161429451632779 31 0.24645717806531117 32 0.030807147258163897 33 0.030807147258163897 34 0.0 35 0.030807147258163897 36 0.030807147258163897 37 0.18484288354898337 38 0.0 39 0.0 40 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 40 3246.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.15588416512631 #Duplication Level Percentage of deduplicated Percentage of total 1 95.74982722874914 85.36660505237215 2 2.6952315134761573 4.805914972273567 3 0.6219765031098825 1.6635859519408502 4 0.27643400138217 0.9858287122612447 5 0.24187975120939878 1.0782501540357363 6 0.10366275051831375 0.5545286506469501 7 0.0691085003455425 0.43130006161429446 8 0.0 0.0 9 0.0 0.0 >10 0.24187975120939878 5.113986444855207 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TGCGGAGGGATGAGATGGACTTAAAGGAATTGACGGGTAC 41 1.2630930375847198 No Hit TGCGGAGGGATGAGATGGACTTAAATGAATTGACGGGTAC 35 1.0782501540357363 No Hit TGCGGAGGGATGAGATGGCCTTAAAGGAATTGACGGGTAC 24 0.7393715341959335 No Hit TGCGGAGGGATGAGATGGACTCAAAGGAATTGACGGGTAC 21 0.6469500924214419 No Hit TGCGGAGGGATGAGATGGCCTTAAATGAATTGACGGGTAC 19 0.5853357979051139 No Hit TGCGGAGGGATGAGATGGACTCAAATGAATTGACGGGTAC 14 0.43130006161429446 No Hit TGCGGAGGGATGAGATGGCCTCAAAGGAATTGACGGGTAC 12 0.36968576709796674 No Hit TGCGGAGGGATGAGATGGCCTTAAAGGAATTGACCGGTAC 7 0.21565003080714723 No Hit TGCGGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT 7 0.21565003080714723 No Hit TGCGGAGGGATGAGATGGACTTACAGGAATTGACGGGTAC 6 0.18484288354898337 No Hit TGCGGAGGGATGAGATGGCCTCAAATGAATTGACGGGTAC 6 0.18484288354898337 No Hit TGCGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC 6 0.18484288354898337 No Hit TGCGGAGGGCTCGTCTTAATCAGCAACATCTTTTGATACA 5 0.15403573629081946 No Hit TGCGGAGGGATGAGCTGGACTTAAAGGAATTGACGGGTAC 5 0.15403573629081946 No Hit TGCGGAGGGATGAGATGGACTCAAATGAATTGACCGGTAC 5 0.15403573629081946 No Hit TGCGGAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT 5 0.15403573629081946 No Hit TGCGGAGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.15403573629081946 No Hit TGCGGAGGGATGAGATGGCCTTCAATGAATTGACGGGTAC 5 0.15403573629081946 No Hit TGCGGAGGGATGAGATGGCCTTACATGAATTGACGGGTAC 5 0.15403573629081946 No Hit TGCGGAGGGATGAGATGGCCTCCAAGGAATTGACGGGTAC 4 0.12322858903265559 No Hit TGCGGAGGGTCAGTCGATTTTTTTTTTTTTTTTTTTTTTT 4 0.12322858903265559 No Hit TGCGGAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4 0.12322858903265559 No Hit TGCGGAGGGATGAGATGGCCTCAAATGAATTGACCGGTAC 4 0.12322858903265559 No Hit TGCGGAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4 0.12322858903265559 No Hit TGCGGAGGGATGAGATGGACTTAAAGGACTTGACGGGTAC 4 0.12322858903265559 No Hit TGCGGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4 0.12322858903265559 No Hit TGCGGAGGGTGCGGAGGGATGAGATGGACTTAAATGAATT 4 0.12322858903265559 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.0 0.0 17 0.0 0.0 0.0 0.0 0.0 18 0.0 0.0 0.0 0.0 0.0 19 0.0 0.0 0.0 0.0 0.0 20 0.0 0.0 0.0 0.0 0.0 21 0.0 0.0 0.0 0.0 0.0 22 0.0 0.0 0.0 0.0 0.0 23 0.0 0.0 0.0 0.0 0.0 24 0.0 0.0 0.0 0.0 0.0 25 0.0 0.0 0.0 0.0 0.0 26 0.0 0.0 0.0 0.0 0.0 27 0.0 0.0 0.0 0.0 0.0 28 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGGGGG 30 1.0989528E-5 33.890625 6 GGACTCA 30 1.0989528E-5 33.890625 17 ACTCAAA 25 1.6437449E-4 33.890625 19 TGGACTC 30 1.0989528E-5 33.890625 16 GGAGGGT 20 0.002433752 33.890625 4 GGAGGGC 20 0.002433752 33.890625 4 GAGGGGT 20 0.002433752 33.890625 5 GAGGGGG 50 1.9463187E-10 33.890625 5 GAGGGGA 45 3.0613592E-9 33.890625 5 GAGGGAG 20 0.002433752 33.890625 5 GACTCAA 25 1.6437449E-4 33.890625 18 GGAGGGG 140 0.0 32.680244 4 TGCGGAG 350 0.0 30.985712 1 GCGGAGG 345 0.0 30.943613 2 CGGAGGG 335 0.0 30.855644 3 GAGGGAA 35 3.159495E-5 29.049107 5 GGAGGGA 155 0.0 28.424395 4 CGGGTAC 30 4.7970266E-4 28.242188 34 GGATGAG 50 3.3795004E-7 27.1125 8 GACGGGT 25 0.007260951 27.1125 32 >>END_MODULE