FastQCFastQC Report
Fri 17 Jun 2016
SRR1525931_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525931_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1211
Sequences flagged as poor quality0
Sequence length40
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCGGAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT201.6515276630883566No Hit
TGCGGAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG90.7431874483897605No Hit
TGCGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC80.6606110652353426No Hit
TGCGGAGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG60.495458298926507No Hit
TGCGGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.495458298926507No Hit
TGCGGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGA60.495458298926507No Hit
TGCGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG60.495458298926507No Hit
TGCGGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT40.3303055326176713No Hit
TGCGGAGGGGAGCCGTCTGTTAGCATACCTAAGGTTATCA40.3303055326176713No Hit
TGCGGAGGGGGGAGCTGGTGAGATGGCTCAGTGGGTAAGA30.2477291494632535No Hit
TGCGGAGGGGTTTGTTTGTTTGTTTGTTTAATTTAATAAT30.2477291494632535No Hit
TGCGGAGGGTAAAAGATAATCTTCTGAGATGAGTTTTTGT30.2477291494632535No Hit
TGCGGAGGGGGTCTAAAATAACAAAAACAAACAAATCCCA30.2477291494632535No Hit
TGCGGAGGGATTTACAGTTGGTGGTCTAACCGGAATTGTT30.2477291494632535No Hit
TGCGGAGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT30.2477291494632535No Hit
TGCGGAGGGGTTGTATGTATTGATGTACAGCCTTGAGTGT30.2477291494632535No Hit
TGCGGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT30.2477291494632535No Hit
TGCGGAGGGGCAATAATGTCTATTGGCTTTCTAGGCTTTA30.2477291494632535No Hit
TGCGGAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGC30.2477291494632535No Hit
TGCGGAGGGGTGACCTGAGTATACAAGTACAATTCTCCAA30.2477291494632535No Hit
TGCGGAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGA20.16515276630883566No Hit
TGCGGAGGGTCACAGACCCTCTTCTGAGATGAGTTTTTGT20.16515276630883566No Hit
TGCGGAGGGGTCTCTTCTGTTTAACAGCTTGCAATTGTAA20.16515276630883566No Hit
TGCGGAGGGTCAAAGATCCTCTGAAGGAGATTGGGCGGAT20.16515276630883566No Hit
TGCGGAGGGGCTGGATTCAGGAGTTTACCCTCCTGGTTCT20.16515276630883566No Hit
TGCGGAGGGCCCAAGATACAATTTGCAAAACACAAGAAAA20.16515276630883566No Hit
TGCGGAGGGGTTGCATATAGACCAATTTCTGCATCAGTGA20.16515276630883566No Hit
TGCGGAGGGGTGTTCTTTATTAATATCCTAACACTCCTCG20.16515276630883566No Hit
TGCGGAGGGGCCAAGATTTTAACTTTCTGCATAAACCTAA20.16515276630883566No Hit
TGCGGAGGGGCTTTCCGGTGGAGGAGTCTGGAGACGACGA20.16515276630883566No Hit
TGCGGAGGGATTTAAAGTTGGTGGTCTAACCGGAATTGTT20.16515276630883566No Hit
TGCGGAGGGGATCCTTTTCTGAGATGAGTTTTTGTTCAAT20.16515276630883566No Hit
TGCGGAGGGGATTGGGCGGATTGAACAAAAACTCATCTCA20.16515276630883566No Hit
TGCGGAGGGATCAATGGGAGCAGTGTTTGCTATCATAGCA20.16515276630883566No Hit
TGCGGAGGGAGGTTTTGGTTTTTTGGTTTTTGTTTTGTTT20.16515276630883566No Hit
TGCGGAGGGAACTTTTTTCTGCTGAAGGTCAAGGATGGGA20.16515276630883566No Hit
TGCGGAGGGGTGATACCTAAAACAGGGTTTTACTGTAAGC20.16515276630883566No Hit
TGCGGAGGGGGATTTGTTCACTGATTCCCATTATTTTCAG20.16515276630883566No Hit
TGCGGAGGGATTCAAAAAAAAAAAAAAAAAAAAAAAAAAA20.16515276630883566No Hit
TGCGGAGGGAGGAGAAGGCAAAACTGTGAATCTCAGCAGC20.16515276630883566No Hit
TGCGGAGGGCAGTCTCTTGTCTCAGGCTCTCTGCCTTGTT20.16515276630883566No Hit
TGCGGAGGGAGTGAAATTTAGCTCATTGAAATGTAAGTAT20.16515276630883566No Hit
TGCGGAGGGAGAGCTAGTGAGGCTGGCTTTTTCCTCCAGG20.16515276630883566No Hit
TGCGGAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGC20.16515276630883566No Hit
TGCGGAGGGGGTAGAAAGTTTTTTCATAGAAGATGTATAA20.16515276630883566No Hit
TGCGGAGGGACCTTTTAAGTTAGTGTCTTTTTGATATTCA20.16515276630883566No Hit
TGCGGAGGGTAACAGATCCTCTTCTGAGATGAGTTTTTGT20.16515276630883566No Hit
TGCGGAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.16515276630883566No Hit
TGCGGAGGGAGTTATGGTTCCTTTGGTCGCTCGCTTCTCT20.16515276630883566No Hit
TGCGGAGGGCATGGCAGTAAGCTTTGATGTTTGGTGGGAG20.16515276630883566No Hit
TGCGGAGGGATTTCTAGCTTGTTGAAACCCAAAACTAATC20.16515276630883566No Hit
TGCGGAGGGCTGCTAGTTGCTGATCAGCCAGTCAGTTCAT20.16515276630883566No Hit
TGCGGAGGGGAGGTAGGAGGTCCTGGGTGTCTTTGGACGT20.16515276630883566No Hit
TGCGGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.16515276630883566No Hit
TGCGGAGGGGAATAGAAAATATATTCTGTTGAGTATTTAA20.16515276630883566No Hit
TGCGGAGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACT20.16515276630883566No Hit
TGCGGAGGGGCTAATGCCAGTTTAAAACTCCCAGGTTAAG20.16515276630883566No Hit
TGCGGAGGGACTGTCAGATGGCCATATGTGCATTGTTCCT20.16515276630883566No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGGG1100.034.03
AGGGTCA305.9052636E-634.06
GCGGAGG1200.034.02
GGAGGGA251.0617067E-434.04
TGCGGAG1200.034.01
ATCCTCT200.001823720633.99999616
GATCCTC200.001823720633.99999615
GGAGGGT353.1267882E-733.9999964
GAGGGTC353.1267882E-733.9999965
AGATCCT200.001823720633.99999614
GGAGGGG502.0991138E-930.5999984
Produced by FastQC (version 0.11.5)