##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1525931_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1211 Sequences flagged as poor quality 0 Sequence length 40 %GC 50 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 15.805945499587118 4.0 4.0 30.0 4.0 33.0 2 9.635012386457474 4.0 4.0 13.0 4.0 26.0 3 8.310487200660612 4.0 4.0 10.0 4.0 20.0 4 8.711808422791082 4.0 4.0 10.0 4.0 24.0 5 8.605284888521883 4.0 4.0 10.0 4.0 24.0 6 8.489677952105698 4.0 4.0 9.0 4.0 24.0 7 9.053674649050372 4.0 4.0 9.0 4.0 27.0 8 9.046242774566474 4.0 4.0 9.0 4.0 27.0 9 8.218001651527663 4.0 4.0 4.0 4.0 23.0 10 7.616019818331957 4.0 4.0 4.0 4.0 21.0 11 8.90916597853014 4.0 4.0 4.0 4.0 28.0 12 9.006606110652353 4.0 4.0 4.0 4.0 28.0 13 8.566473988439306 4.0 4.0 4.0 4.0 26.0 14 8.77869529314616 4.0 4.0 4.0 4.0 28.0 15 8.524360033030554 4.0 4.0 4.0 4.0 27.0 16 7.6061106523534265 4.0 4.0 4.0 4.0 23.0 17 7.254335260115607 4.0 4.0 4.0 4.0 20.0 18 7.193228736581338 4.0 4.0 4.0 4.0 21.0 19 6.910817506193228 4.0 4.0 4.0 4.0 19.0 20 7.233691164327002 4.0 4.0 4.0 4.0 22.0 21 6.576383154417837 4.0 4.0 4.0 4.0 17.0 22 6.364987613542527 4.0 4.0 4.0 4.0 15.0 23 6.477291494632535 4.0 4.0 4.0 4.0 16.0 24 6.338563170933113 4.0 4.0 4.0 4.0 15.0 25 6.518579686209744 4.0 4.0 4.0 4.0 17.0 26 6.654830718414534 4.0 4.0 4.0 4.0 17.0 27 6.942196531791907 4.0 4.0 4.0 4.0 20.0 28 6.908340214698597 4.0 4.0 4.0 4.0 20.0 29 6.42279108175062 4.0 4.0 4.0 4.0 14.0 30 6.582163501238646 4.0 4.0 4.0 4.0 16.0 31 6.990916597853014 4.0 4.0 4.0 4.0 20.0 32 6.982658959537572 4.0 4.0 4.0 4.0 21.0 33 6.851362510322048 4.0 4.0 4.0 4.0 18.0 34 6.928158546655657 4.0 4.0 4.0 4.0 20.0 35 6.985962014863749 4.0 4.0 4.0 4.0 21.0 36 7.0520231213872835 4.0 4.0 4.0 4.0 22.0 37 7.092485549132948 4.0 4.0 4.0 4.0 21.0 38 6.815854665565648 4.0 4.0 4.0 4.0 17.0 39 6.757225433526012 4.0 4.0 4.0 4.0 16.0 40 6.838976052848885 4.0 4.0 4.0 4.0 16.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2 1 6.326388888888889 2 2 -4.064814814814816 2 3 -2.2430555555555545 2 4 -3.962962962962963 2 5 -2.5486111111111107 2 6 -0.36574074074074225 2 7 2.3240740740740744 2 8 0.05092592592592737 2 9 -4.6967592592592595 2 10 -1.5740740740740735 2 11 -3.0277777777777786 2 12 0.9699074074074083 2 13 2.2638888888888875 2 14 -2.0092592592592604 2 15 2.1296296296296298 2 16 -1.0601851851851851 2 17 4.025462962962963 2 18 2.8611111111111107 2 19 1.0671296296296298 2 20 3.291666666666667 2 21 1.1782407407407405 2 22 -0.06944444444444464 2 23 3.914351851851851 2 24 -0.14351851851851816 2 25 5.465277777777777 2 26 5.224537037037036 2 27 2.662037037037037 2 28 -2.018518518518518 2 29 2.960648148148148 2 30 0.956018518518519 2 31 4.773148148148149 2 32 5.912037037037037 2 33 4.789351851851852 2 34 5.368055555555555 2 35 5.548611111111111 2 36 7.159722222222223 2 37 7.560185185185185 2 38 8.07175925925926 2 39 7.298611111111112 2 40 7.722222222222221 3 1 -11.67361111111111 3 2 -5.398148148148149 3 3 -4.5763888888888875 3 4 -4.62962962962963 3 5 -4.548611111111111 3 6 -5.032407407407408 3 7 -5.342592592592592 3 8 -5.2824074074074066 3 9 -4.6967592592592595 3 10 -3.9074074074074066 3 11 -6.027777777777779 3 12 -6.030092592592592 3 13 -5.402777777777779 3 14 -5.675925925925927 3 15 -5.537037037037036 3 16 -3.060185185185185 3 17 -3.307870370370371 3 18 -2.8055555555555554 3 19 -2.2662037037037033 3 20 -2.708333333333333 3 21 -2.1550925925925926 3 22 -2.4027777777777777 3 23 -2.085648148148149 3 24 -2.143518518518518 3 25 -2.2013888888888893 3 26 -2.4421296296296298 3 27 -2.6712962962962967 3 28 -3.018518518518518 3 29 -2.372685185185186 3 30 -2.377314814814814 3 31 -3.226851851851851 3 32 -3.087962962962963 3 33 -3.210648148148148 3 34 -3.2986111111111107 3 35 -3.4513888888888893 3 36 -3.1736111111111107 3 37 -2.773148148148149 3 38 -2.5949074074074074 3 39 -3.0347222222222223 3 40 -2.9444444444444446 5 1 13.993055555555557 5 2 8.268518518518519 5 3 1.4236111111111125 5 4 0.03703703703703631 5 5 -0.2152777777777768 5 6 -2.6990740740740753 5 7 -1.0092592592592577 5 8 -0.6157407407407405 5 9 0.9699074074074066 5 10 1.7592592592592595 5 11 -1.6944444444444446 5 12 0.9699074074074083 5 13 3.5972222222222214 5 14 -5.675925925925927 5 15 -5.537037037037036 5 16 -3.060185185185185 5 17 -3.307870370370371 5 18 -2.8055555555555554 5 19 -2.2662037037037033 5 20 -2.708333333333333 5 21 -2.1550925925925926 5 22 -2.4027777777777777 5 23 -2.085648148148149 5 24 -2.143518518518518 5 25 -2.2013888888888893 5 26 -2.4421296296296298 5 27 -2.6712962962962967 5 28 -3.018518518518518 5 29 -2.372685185185186 5 30 -2.377314814814814 5 31 -3.226851851851851 5 32 -3.087962962962963 5 33 -3.210648148148148 5 34 -3.2986111111111107 5 35 -3.4513888888888893 5 36 -3.1736111111111107 5 37 -2.773148148148149 5 38 -2.5949074074074074 5 39 -3.0347222222222223 5 40 -2.9444444444444446 8 1 1.3263888888888893 8 2 -0.8981481481481488 8 3 0.9236111111111125 8 4 1.8703703703703702 8 5 2.9513888888888893 8 6 6.967592592592592 8 7 4.157407407407408 8 8 4.2175925925925934 8 9 6.8032407407407405 8 10 4.5925925925925934 8 11 -2.5277777777777786 8 12 -3.0300925925925917 8 13 -2.4027777777777786 8 14 -0.17592592592592737 8 15 5.462962962962964 8 16 7.939814814814815 8 17 -0.8078703703703711 8 18 1.1944444444444446 8 19 3.7337962962962967 8 20 0.791666666666667 8 21 7.844907407407407 8 22 7.097222222222222 8 23 7.414351851851851 8 24 -1.1435185185185182 8 25 5.798611111111111 8 26 9.05787037037037 8 27 2.8287037037037033 8 28 3.981481481481482 8 29 8.127314814814813 8 30 1.122685185185186 8 31 12.273148148148149 8 32 5.912037037037037 8 33 11.289351851851851 8 34 11.70138888888889 8 35 11.04861111111111 8 36 11.82638888888889 8 37 5.726851851851851 8 38 2.4050925925925926 8 39 12.465277777777779 8 40 11.555555555555555 9 1 -3.0069444444444446 9 2 -5.398148148148149 9 3 -4.5763888888888875 9 4 -4.62962962962963 9 5 -4.548611111111111 9 6 -5.032407407407408 9 7 -5.342592592592592 9 8 -5.2824074074074066 9 9 -4.6967592592592595 9 10 -3.9074074074074066 9 11 -6.027777777777779 9 12 -6.030092592592592 9 13 -5.402777777777779 9 14 -5.675925925925927 9 15 -5.537037037037036 9 16 -3.060185185185185 9 17 -3.307870370370371 9 18 -2.8055555555555554 9 19 -2.2662037037037033 9 20 -2.708333333333333 9 21 -2.1550925925925926 9 22 -2.4027777777777777 9 23 -2.085648148148149 9 24 -2.143518518518518 9 25 -2.2013888888888893 9 26 -2.4421296296296298 9 27 -2.6712962962962967 9 28 -3.018518518518518 9 29 -2.372685185185186 9 30 -2.377314814814814 9 31 -3.226851851851851 9 32 -3.087962962962963 9 33 -3.210648148148148 9 34 -3.2986111111111107 9 35 -3.4513888888888893 9 36 -3.1736111111111107 9 37 -2.773148148148149 9 38 -2.5949074074074074 9 39 -3.0347222222222223 9 40 -2.9444444444444446 13 1 13.32638888888889 13 2 3.601851851851851 13 3 -0.5763888888888875 13 4 1.3703703703703702 13 5 5.451388888888889 13 6 7.967592592592592 13 7 8.657407407407408 13 8 8.717592592592593 13 9 8.30324074074074 13 10 8.092592592592593 13 11 26.97222222222222 13 12 26.96990740740741 13 13 22.59722222222222 13 14 19.324074074074073 13 15 23.462962962962962 13 16 16.939814814814817 13 17 23.69212962962963 13 18 18.194444444444443 13 19 19.733796296296298 13 20 17.291666666666668 13 21 17.84490740740741 13 22 7.597222222222222 13 23 12.914351851851851 13 24 17.85648148148148 13 25 8.79861111111111 13 26 14.55787037037037 13 27 7.328703703703703 13 28 19.98148148148148 13 29 8.627314814814813 13 30 8.622685185185187 13 31 23.77314814814815 13 32 23.912037037037038 13 33 28.78935185185185 13 34 28.70138888888889 13 35 24.54861111111111 13 36 26.82638888888889 13 37 19.22685185185185 13 38 25.40509259259259 13 39 19.96527777777778 13 40 11.055555555555555 15 1 20.32638888888889 15 2 26.60185185185185 15 3 27.423611111111114 15 4 27.37037037037037 15 5 27.45138888888889 15 6 23.96759259259259 15 7 25.65740740740741 15 8 25.717592592592595 15 9 21.30324074074074 15 10 15.092592592592593 15 11 -0.027777777777778567 15 12 8.969907407407408 15 13 18.59722222222222 15 14 9.324074074074073 15 15 23.462962962962962 15 16 -3.060185185185185 15 17 -3.307870370370371 15 18 -2.8055555555555554 15 19 -2.2662037037037033 15 20 -2.708333333333333 15 21 -2.1550925925925926 15 22 -2.4027777777777777 15 23 -2.085648148148149 15 24 -2.143518518518518 15 25 -2.2013888888888893 15 26 -2.4421296296296298 15 27 -2.6712962962962967 15 28 -3.018518518518518 15 29 -2.372685185185186 15 30 -2.377314814814814 15 31 -3.226851851851851 15 32 -3.087962962962963 15 33 -3.210648148148148 15 34 -3.2986111111111107 15 35 -3.4513888888888893 15 36 -3.1736111111111107 15 37 -2.773148148148149 15 38 -2.5949074074074074 15 39 -3.0347222222222223 15 40 -2.9444444444444446 16 1 15.82638888888889 16 2 7.101851851851851 16 3 4.9236111111111125 16 4 14.37037037037037 16 5 15.95138888888889 16 6 15.467592592592592 16 7 13.157407407407408 16 8 20.217592592592595 16 9 14.30324074074074 16 10 11.092592592592593 16 11 19.97222222222222 16 12 18.46990740740741 16 13 22.09722222222222 16 14 25.324074074074073 16 15 9.462962962962964 16 16 6.939814814814815 16 17 8.19212962962963 16 18 1.1944444444444446 16 19 1.2337962962962967 16 20 9.791666666666668 16 21 3.8449074074074074 16 22 3.0972222222222223 16 23 2.914351851851851 16 24 6.356481481481482 16 25 8.29861111111111 16 26 12.05787037037037 16 27 9.328703703703702 16 28 9.481481481481481 16 29 10.627314814814813 16 30 13.122685185185187 16 31 8.773148148148149 16 32 9.412037037037038 16 33 12.789351851851851 16 34 6.201388888888889 16 35 11.04861111111111 16 36 -3.1736111111111107 16 37 -2.773148148148149 16 38 -2.5949074074074074 16 39 -3.0347222222222223 16 40 -2.9444444444444446 18 1 15.32638888888889 18 2 6.268518518518517 18 3 5.4236111111111125 18 4 11.703703703703702 18 5 15.45138888888889 18 6 16.96759259259259 18 7 16.65740740740741 18 8 17.38425925925926 18 9 16.30324074074074 18 10 14.425925925925926 18 11 16.97222222222222 18 12 19.30324074074074 18 13 12.597222222222221 18 14 14.657407407407405 18 15 22.129629629629633 18 16 2.273148148148149 18 17 1.6921296296296289 18 18 0.5277777777777777 18 19 1.7337962962962967 18 20 4.291666666666667 18 21 2.5115740740740735 18 22 3.5972222222222223 18 23 6.914351851851851 18 24 2.1898148148148158 18 25 4.465277777777777 18 26 0.2245370370370372 18 27 6.328703703703703 18 28 6.648148148148148 18 29 0.627314814814814 18 30 2.95601851851852 18 31 5.773148148148149 18 32 3.5787037037037033 18 33 3.789351851851852 18 34 3.3680555555555554 18 35 6.215277777777777 18 36 2.8263888888888893 18 37 2.226851851851851 18 38 4.071759259259259 18 39 1.9652777777777777 18 40 6.388888888888889 19 1 1.3263888888888893 19 2 -3.398148148148149 19 3 -0.07638888888888751 19 4 3.3703703703703702 19 5 2.9513888888888893 19 6 9.467592592592592 19 7 6.657407407407408 19 8 6.2175925925925934 19 9 5.3032407407407405 19 10 6.0925925925925934 19 11 9.472222222222221 19 12 9.469907407407408 19 13 10.097222222222221 19 14 -1.1759259259259274 19 15 9.962962962962964 19 16 10.439814814814815 19 17 10.19212962962963 19 18 3.1944444444444446 19 19 10.733796296296298 19 20 11.791666666666668 19 21 -2.1550925925925926 19 22 -2.4027777777777777 19 23 -2.085648148148149 19 24 -2.143518518518518 19 25 -2.2013888888888893 19 26 -2.4421296296296298 19 27 -2.6712962962962967 19 28 -3.018518518518518 19 29 -2.372685185185186 19 30 -2.377314814814814 19 31 -3.226851851851851 19 32 -3.087962962962963 19 33 -3.210648148148148 19 34 -3.2986111111111107 19 35 -3.4513888888888893 19 36 -3.1736111111111107 19 37 -2.773148148148149 19 38 -2.5949074074074074 19 39 -3.0347222222222223 19 40 -2.9444444444444446 20 1 -11.67361111111111 20 2 -5.398148148148149 20 3 -4.5763888888888875 20 4 -4.62962962962963 20 5 -4.548611111111111 20 6 -5.032407407407408 20 7 -5.342592592592592 20 8 -5.2824074074074066 20 9 -4.6967592592592595 20 10 -3.9074074074074066 20 11 -6.027777777777779 20 12 -6.030092592592592 20 13 -5.402777777777779 20 14 -5.675925925925927 20 15 -5.537037037037036 20 16 -3.060185185185185 20 17 -3.307870370370371 20 18 -2.8055555555555554 20 19 -2.2662037037037033 20 20 -2.708333333333333 20 21 -2.1550925925925926 20 22 -2.4027777777777777 20 23 -2.085648148148149 20 24 -2.143518518518518 20 25 -2.2013888888888893 20 26 -2.4421296296296298 20 27 -2.6712962962962967 20 28 -3.018518518518518 20 29 -2.372685185185186 20 30 -2.377314814814814 20 31 -3.226851851851851 20 32 -3.087962962962963 20 33 -3.210648148148148 20 34 -3.2986111111111107 20 35 -3.4513888888888893 20 36 -3.1736111111111107 20 37 -2.773148148148149 20 38 -2.5949074074074074 20 39 -3.0347222222222223 20 40 -2.9444444444444446 23 1 14.32638888888889 23 2 -5.398148148148149 23 3 -4.5763888888888875 23 4 -4.62962962962963 23 5 -4.548611111111111 23 6 -5.032407407407408 23 7 -5.342592592592592 23 8 -5.2824074074074066 23 9 -4.6967592592592595 23 10 -3.9074074074074066 23 11 -6.027777777777779 23 12 -6.030092592592592 23 13 -5.402777777777779 23 14 -5.675925925925927 23 15 -5.537037037037036 23 16 -3.060185185185185 23 17 -3.307870370370371 23 18 -2.8055555555555554 23 19 -2.2662037037037033 23 20 -2.708333333333333 23 21 -2.1550925925925926 23 22 -2.4027777777777777 23 23 -2.085648148148149 23 24 -2.143518518518518 23 25 -2.2013888888888893 23 26 -2.4421296296296298 23 27 -2.6712962962962967 23 28 -3.018518518518518 23 29 -2.372685185185186 23 30 -2.377314814814814 23 31 -3.226851851851851 23 32 -3.087962962962963 23 33 -3.210648148148148 23 34 -3.2986111111111107 23 35 -3.4513888888888893 23 36 -3.1736111111111107 23 37 -2.773148148148149 23 38 -2.5949074074074074 23 39 -3.0347222222222223 23 40 -2.9444444444444446 26 1 13.32638888888889 26 2 7.601851851851851 26 3 7.4236111111111125 26 4 -2.6296296296296298 26 5 9.45138888888889 26 6 9.967592592592592 26 7 12.657407407407408 26 8 12.717592592592593 26 9 13.30324074074074 26 10 9.092592592592593 26 11 24.97222222222222 26 12 24.96990740740741 26 13 25.59722222222222 26 14 25.324074074074073 26 15 19.462962962962962 26 16 9.939814814814815 26 17 9.69212962962963 26 18 10.194444444444445 26 19 12.733796296296298 26 20 14.291666666666668 26 21 -1.1550925925925926 26 22 6.597222222222222 26 23 0.9143518518518512 26 24 3.856481481481482 26 25 3.7986111111111107 26 26 8.55787037037037 26 27 11.328703703703702 26 28 20.98148148148148 26 29 1.627314814814814 26 30 11.622685185185187 26 31 21.77314814814815 26 32 14.912037037037038 26 33 27.78935185185185 26 34 15.70138888888889 26 35 21.54861111111111 26 36 27.82638888888889 26 37 13.226851851851851 26 38 5.405092592592593 26 39 25.96527777777778 26 40 26.055555555555557 29 1 11.32638888888889 29 2 19.60185185185185 29 3 9.423611111111112 29 4 11.37037037037037 29 5 15.45138888888889 29 6 6.967592592592592 29 7 10.657407407407408 29 8 4.7175925925925934 29 9 3.3032407407407405 29 10 11.092592592592593 29 11 17.97222222222222 29 12 17.96990740740741 29 13 7.597222222222221 29 14 20.324074074074073 29 15 23.462962962962962 29 16 -3.060185185185185 29 17 -3.307870370370371 29 18 -2.8055555555555554 29 19 -2.2662037037037033 29 20 -2.708333333333333 29 21 -2.1550925925925926 29 22 -2.4027777777777777 29 23 -2.085648148148149 29 24 -2.143518518518518 29 25 -2.2013888888888893 29 26 -2.4421296296296298 29 27 -2.6712962962962967 29 28 -3.018518518518518 29 29 -2.372685185185186 29 30 -2.377314814814814 29 31 -3.226851851851851 29 32 -3.087962962962963 29 33 -3.210648148148148 29 34 -3.2986111111111107 29 35 -3.4513888888888893 29 36 -3.1736111111111107 29 37 -2.773148148148149 29 38 -2.5949074074074074 29 39 -3.0347222222222223 29 40 -2.9444444444444446 33 1 1.3263888888888893 33 2 -5.398148148148149 33 3 -4.5763888888888875 33 4 -4.62962962962963 33 5 -4.548611111111111 33 6 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8.19212962962963 109 18 10.694444444444445 109 19 -2.2662037037037033 109 20 -2.708333333333333 109 21 -2.1550925925925926 109 22 -2.4027777777777777 109 23 -2.085648148148149 109 24 -2.143518518518518 109 25 -2.2013888888888893 109 26 -2.4421296296296298 109 27 -2.6712962962962967 109 28 -3.018518518518518 109 29 -2.372685185185186 109 30 -2.377314814814814 109 31 -3.226851851851851 109 32 -3.087962962962963 109 33 -3.210648148148148 109 34 -3.2986111111111107 109 35 -3.4513888888888893 109 36 -3.1736111111111107 109 37 -2.773148148148149 109 38 -2.5949074074074074 109 39 -3.0347222222222223 109 40 -2.9444444444444446 110 1 -11.67361111111111 110 2 -5.398148148148149 110 3 -4.5763888888888875 110 4 -4.62962962962963 110 5 -4.548611111111111 110 6 -5.032407407407408 110 7 -5.342592592592592 110 8 -5.2824074074074066 110 9 -4.6967592592592595 110 10 -3.9074074074074066 110 11 -6.027777777777779 110 12 -6.030092592592592 110 13 -5.402777777777779 110 14 -5.675925925925927 110 15 -5.537037037037036 110 16 -3.060185185185185 110 17 -3.307870370370371 110 18 -2.8055555555555554 110 19 -2.2662037037037033 110 20 -2.708333333333333 110 21 -2.1550925925925926 110 22 -2.4027777777777777 110 23 -2.085648148148149 110 24 -2.143518518518518 110 25 -2.2013888888888893 110 26 -2.4421296296296298 110 27 -2.6712962962962967 110 28 -3.018518518518518 110 29 -2.372685185185186 110 30 -2.377314814814814 110 31 -3.226851851851851 110 32 -3.087962962962963 110 33 -3.210648148148148 110 34 -3.2986111111111107 110 35 -3.4513888888888893 110 36 -3.1736111111111107 110 37 -2.773148148148149 110 38 -2.5949074074074074 110 39 -3.0347222222222223 110 40 -2.9444444444444446 111 1 14.32638888888889 111 2 12.601851851851851 111 3 12.423611111111112 111 4 5.37037037037037 111 5 -2.5486111111111107 111 6 10.967592592592592 111 7 -2.3425925925925917 111 8 -3.2824074074074066 111 9 6.3032407407407405 111 10 7.0925925925925934 111 11 22.97222222222222 111 12 23.96990740740741 111 13 19.59722222222222 111 14 23.324074074074073 111 15 24.462962962962962 111 16 12.939814814814815 111 17 15.69212962962963 111 18 24.194444444444443 111 19 -2.2662037037037033 111 20 -2.708333333333333 111 21 -2.1550925925925926 111 22 -2.4027777777777777 111 23 -2.085648148148149 111 24 -2.143518518518518 111 25 -2.2013888888888893 111 26 -2.4421296296296298 111 27 -2.6712962962962967 111 28 -3.018518518518518 111 29 -2.372685185185186 111 30 -2.377314814814814 111 31 -3.226851851851851 111 32 -3.087962962962963 111 33 -3.210648148148148 111 34 -3.2986111111111107 111 35 -3.4513888888888893 111 36 -3.1736111111111107 111 37 -2.773148148148149 111 38 -2.5949074074074074 111 39 -3.0347222222222223 111 40 -2.9444444444444446 113 1 -11.67361111111111 113 2 -5.398148148148149 113 3 -4.5763888888888875 113 4 -4.62962962962963 113 5 -4.548611111111111 113 6 -5.032407407407408 113 7 -5.342592592592592 113 8 -5.2824074074074066 113 9 -4.6967592592592595 113 10 -3.9074074074074066 113 11 -6.027777777777779 113 12 -6.030092592592592 113 13 -5.402777777777779 113 14 -5.675925925925927 113 15 -5.537037037037036 113 16 -3.060185185185185 113 17 -3.307870370370371 113 18 -2.8055555555555554 113 19 -2.2662037037037033 113 20 -2.708333333333333 113 21 -2.1550925925925926 113 22 -2.4027777777777777 113 23 -2.085648148148149 113 24 -2.143518518518518 113 25 -2.2013888888888893 113 26 -2.4421296296296298 113 27 -2.6712962962962967 113 28 -3.018518518518518 113 29 -2.372685185185186 113 30 -2.377314814814814 113 31 -3.226851851851851 113 32 -3.087962962962963 113 33 -3.210648148148148 113 34 -3.2986111111111107 113 35 -3.4513888888888893 113 36 -3.1736111111111107 113 37 -2.773148148148149 113 38 -2.5949074074074074 113 39 -3.0347222222222223 113 40 -2.9444444444444446 114 1 -11.67361111111111 114 2 -5.398148148148149 114 3 -4.5763888888888875 114 4 -4.62962962962963 114 5 -4.548611111111111 114 6 -5.032407407407408 114 7 -5.342592592592592 114 8 -5.2824074074074066 114 9 -4.6967592592592595 114 10 -3.9074074074074066 114 11 -6.027777777777779 114 12 -6.030092592592592 114 13 -5.402777777777779 114 14 -5.675925925925927 114 15 -5.537037037037036 114 16 -3.060185185185185 114 17 -3.307870370370371 114 18 -2.8055555555555554 114 19 -2.2662037037037033 114 20 -2.708333333333333 114 21 -2.1550925925925926 114 22 -2.4027777777777777 114 23 -2.085648148148149 114 24 -2.143518518518518 114 25 -2.2013888888888893 114 26 -2.4421296296296298 114 27 -2.6712962962962967 114 28 -3.018518518518518 114 29 -2.372685185185186 114 30 -2.377314814814814 114 31 -3.226851851851851 114 32 -3.087962962962963 114 33 -3.210648148148148 114 34 -3.2986111111111107 114 35 -3.4513888888888893 114 36 -3.1736111111111107 114 37 -2.773148148148149 114 38 -2.5949074074074074 114 39 -3.0347222222222223 114 40 -2.9444444444444446 116 1 14.32638888888889 116 2 -5.398148148148149 116 3 -4.5763888888888875 116 4 -4.62962962962963 116 5 -4.548611111111111 116 6 -5.032407407407408 116 7 -5.342592592592592 116 8 -5.2824074074074066 116 9 -4.6967592592592595 116 10 -3.9074074074074066 116 11 -6.027777777777779 116 12 -6.030092592592592 116 13 -5.402777777777779 116 14 -5.675925925925927 116 15 -5.537037037037036 116 16 -3.060185185185185 116 17 -3.307870370370371 116 18 -2.8055555555555554 116 19 -2.2662037037037033 116 20 -2.708333333333333 116 21 -2.1550925925925926 116 22 -2.4027777777777777 116 23 -2.085648148148149 116 24 -2.143518518518518 116 25 -2.2013888888888893 116 26 -2.4421296296296298 116 27 -2.6712962962962967 116 28 -3.018518518518518 116 29 -2.372685185185186 116 30 -2.377314814814814 116 31 -3.226851851851851 116 32 -3.087962962962963 116 33 -3.210648148148148 116 34 -3.2986111111111107 116 35 -3.4513888888888893 116 36 -3.1736111111111107 116 37 -2.773148148148149 116 38 -2.5949074074074074 116 39 -3.0347222222222223 116 40 -2.9444444444444446 119 1 4.326388888888889 119 2 0.6018518518518512 119 3 10.923611111111112 119 4 8.37037037037037 119 5 8.45138888888889 119 6 9.467592592592592 119 7 1.1574074074074083 119 8 10.717592592592593 119 9 8.80324074074074 119 10 0.5925925925925934 119 11 7.472222222222221 119 12 7.469907407407408 119 13 7.597222222222221 119 14 7.824074074074073 119 15 -5.537037037037036 119 16 -3.060185185185185 119 17 -3.307870370370371 119 18 -2.8055555555555554 119 19 -2.2662037037037033 119 20 -2.708333333333333 119 21 -2.1550925925925926 119 22 -2.4027777777777777 119 23 -2.085648148148149 119 24 -2.143518518518518 119 25 -2.2013888888888893 119 26 -2.4421296296296298 119 27 -2.6712962962962967 119 28 -3.018518518518518 119 29 -2.372685185185186 119 30 -2.377314814814814 119 31 -3.226851851851851 119 32 -3.087962962962963 119 33 -3.210648148148148 119 34 -3.2986111111111107 119 35 -3.4513888888888893 119 36 -3.1736111111111107 119 37 -2.773148148148149 119 38 -2.5949074074074074 119 39 -3.0347222222222223 119 40 -2.9444444444444446 >>END_MODULE >>Per sequence quality scores fail #Quality Count 4 812.0 5 36.0 6 19.0 7 26.0 8 13.0 9 21.0 10 21.0 11 33.0 12 18.0 13 24.0 14 11.0 15 8.0 16 4.0 17 4.0 18 7.0 19 19.0 20 21.0 21 28.0 22 30.0 23 18.0 24 12.0 25 9.0 26 9.0 27 2.0 28 0.0 29 3.0 30 2.0 31 0.0 32 0.0 33 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 100.0 0.0 0.0 0.0 3 0.0 0.0 0.0 100.0 4 100.0 0.0 0.0 0.0 5 100.0 0.0 0.0 0.0 6 0.0 100.0 0.0 0.0 7 98.51116625310173 0.5789909015715468 0.3308519437551696 0.5789909015715468 8 97.93388429752066 1.322314049586777 0.3305785123966942 0.4132231404958678 9 97.10024855012428 1.0770505385252693 1.5741507870753937 0.24855012427506215 10 49.5458298926507 23.451692815854667 16.928158546655656 10.074318744838976 11 29.91735537190083 22.975206611570247 23.140495867768596 23.96694214876033 12 29.975227085053675 29.232039636663913 23.121387283236995 17.671345995045417 13 22.625928984310487 26.67217175887696 27.41535920726672 23.286540049545827 14 18.74483897605285 33.360858794384804 28.65400495458299 19.240297274979355 15 31.157024793388427 27.603305785123965 25.123966942148762 16.115702479338843 16 28.57142857142857 37.24194880264244 21.80016515276631 12.386457473162675 17 25.84640792733278 26.34186622625929 34.02146985962015 13.790255986787777 18 23.699421965317917 27.41535920726672 27.250206440957886 21.635012386457472 19 22.625928984310487 29.149463253509495 25.516102394715112 22.708505367464905 20 23.534269199009085 25.26837324525186 32.782824112303885 18.414533443435175 21 23.121387283236995 25.43352601156069 27.250206440957886 24.194880264244425 22 19.983484723369116 22.378199834847234 36.82906688687035 20.809248554913296 23 22.46077621800165 27.580511973575554 34.02146985962015 15.937241948802642 24 20.974401321222132 29.72749793559042 27.002477291494632 22.295623451692816 25 23.038810900082577 29.39719240297275 31.957060280759702 15.606936416184972 26 29.03225806451613 27.626137303556657 25.144747725392886 18.196856906534325 27 19.768403639371382 38.13068651778329 22.8287841191067 19.272125723738625 28 23.553719008264462 32.8099173553719 23.884297520661157 19.75206611570248 29 23.427152317880797 33.77483443708609 26.40728476821192 16.39072847682119 30 23.71900826446281 27.024793388429753 31.074380165289256 18.181818181818183 31 26.011560693641616 31.131296449215522 24.44260941370768 18.414533443435175 32 26.40728476821192 30.4635761589404 25.496688741721858 17.632450331125828 33 26.446280991735538 30.0 27.603305785123965 15.950413223140497 34 20.561519405450042 25.84640792733278 38.97605284888522 14.616019818331957 35 19.240297274979355 29.31461601981833 35.672997522708506 15.772089182493806 36 21.074380165289256 28.677685950413224 32.06611570247934 18.181818181818183 37 23.801652892561982 29.83471074380165 29.75206611570248 16.611570247933884 38 24.132231404958677 30.909090909090907 26.28099173553719 18.677685950413224 39 24.77291494632535 30.14037985136251 22.54335260115607 22.54335260115607 40 22.31404958677686 28.92561983471074 30.66115702479339 18.09917355371901 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 1.5 15 3.0 16 3.0 17 3.0 18 3.0 19 9.5 20 16.0 21 15.0 22 14.0 23 14.0 24 13.0 25 12.0 26 11.5 27 11.0 28 11.0 29 18.0 30 25.0 31 23.0 32 21.0 33 21.0 34 25.0 35 29.0 36 32.0 37 35.0 38 35.0 39 47.0 40 59.0 41 63.0 42 67.0 43 67.0 44 75.5 45 84.0 46 90.0 47 96.0 48 96.0 49 97.0 50 98.0 51 99.0 52 100.0 53 100.0 54 111.0 55 122.0 56 109.5 57 97.0 58 97.0 59 89.0 60 81.0 61 92.5 62 104.0 63 104.0 64 87.0 65 70.0 66 53.5 67 37.0 68 37.0 69 23.5 70 10.0 71 9.5 72 9.0 73 9.0 74 6.5 75 4.0 76 2.5 77 1.0 78 1.0 79 1.5 80 2.0 81 1.5 82 1.0 83 1.0 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.16515276630883566 8 0.08257638315441783 9 0.3303055326176713 10 0.0 11 0.08257638315441783 12 0.0 13 0.0 14 0.0 15 0.08257638315441783 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.16515276630883566 27 0.16515276630883566 28 0.08257638315441783 29 0.2477291494632535 30 0.08257638315441783 31 0.0 32 0.2477291494632535 33 0.08257638315441783 34 0.0 35 0.0 36 0.08257638315441783 37 0.08257638315441783 38 0.08257638315441783 39 0.0 40 0.08257638315441783 >>END_MODULE >>Sequence Length Distribution pass #Length Count 40 1211.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 90.09083402146986 #Duplication Level Percentage of deduplicated Percentage of total 1 94.68377635197068 85.30140379851362 2 3.4830430797433545 6.275805119735756 3 1.008249312557287 2.725020644095789 4 0.18331805682859761 0.6606110652353426 5 0.0 0.0 6 0.36663611365719523 1.981833195706028 7 0.0 0.0 8 0.09165902841429881 0.6606110652353426 9 0.09165902841429881 0.7431874483897605 >10 0.09165902841429881 1.6515276630883566 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TGCGGAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT 20 1.6515276630883566 No Hit TGCGGAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG 9 0.7431874483897605 No Hit TGCGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC 8 0.6606110652353426 No Hit TGCGGAGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG 6 0.495458298926507 No Hit TGCGGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6 0.495458298926507 No Hit TGCGGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGA 6 0.495458298926507 No Hit TGCGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG 6 0.495458298926507 No Hit TGCGGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT 4 0.3303055326176713 No Hit TGCGGAGGGGAGCCGTCTGTTAGCATACCTAAGGTTATCA 4 0.3303055326176713 No Hit TGCGGAGGGGGGAGCTGGTGAGATGGCTCAGTGGGTAAGA 3 0.2477291494632535 No Hit TGCGGAGGGGTTTGTTTGTTTGTTTGTTTAATTTAATAAT 3 0.2477291494632535 No Hit TGCGGAGGGTAAAAGATAATCTTCTGAGATGAGTTTTTGT 3 0.2477291494632535 No Hit TGCGGAGGGGGTCTAAAATAACAAAAACAAACAAATCCCA 3 0.2477291494632535 No Hit TGCGGAGGGATTTACAGTTGGTGGTCTAACCGGAATTGTT 3 0.2477291494632535 No Hit TGCGGAGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT 3 0.2477291494632535 No Hit TGCGGAGGGGTTGTATGTATTGATGTACAGCCTTGAGTGT 3 0.2477291494632535 No Hit TGCGGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT 3 0.2477291494632535 No Hit TGCGGAGGGGCAATAATGTCTATTGGCTTTCTAGGCTTTA 3 0.2477291494632535 No Hit TGCGGAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGC 3 0.2477291494632535 No Hit TGCGGAGGGGTGACCTGAGTATACAAGTACAATTCTCCAA 3 0.2477291494632535 No Hit TGCGGAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGA 2 0.16515276630883566 No Hit TGCGGAGGGTCACAGACCCTCTTCTGAGATGAGTTTTTGT 2 0.16515276630883566 No Hit TGCGGAGGGGTCTCTTCTGTTTAACAGCTTGCAATTGTAA 2 0.16515276630883566 No Hit TGCGGAGGGTCAAAGATCCTCTGAAGGAGATTGGGCGGAT 2 0.16515276630883566 No Hit TGCGGAGGGGCTGGATTCAGGAGTTTACCCTCCTGGTTCT 2 0.16515276630883566 No Hit TGCGGAGGGCCCAAGATACAATTTGCAAAACACAAGAAAA 2 0.16515276630883566 No Hit TGCGGAGGGGTTGCATATAGACCAATTTCTGCATCAGTGA 2 0.16515276630883566 No Hit TGCGGAGGGGTGTTCTTTATTAATATCCTAACACTCCTCG 2 0.16515276630883566 No Hit TGCGGAGGGGCCAAGATTTTAACTTTCTGCATAAACCTAA 2 0.16515276630883566 No Hit TGCGGAGGGGCTTTCCGGTGGAGGAGTCTGGAGACGACGA 2 0.16515276630883566 No Hit TGCGGAGGGATTTAAAGTTGGTGGTCTAACCGGAATTGTT 2 0.16515276630883566 No Hit TGCGGAGGGGATCCTTTTCTGAGATGAGTTTTTGTTCAAT 2 0.16515276630883566 No Hit TGCGGAGGGGATTGGGCGGATTGAACAAAAACTCATCTCA 2 0.16515276630883566 No Hit TGCGGAGGGATCAATGGGAGCAGTGTTTGCTATCATAGCA 2 0.16515276630883566 No Hit TGCGGAGGGAGGTTTTGGTTTTTTGGTTTTTGTTTTGTTT 2 0.16515276630883566 No Hit TGCGGAGGGAACTTTTTTCTGCTGAAGGTCAAGGATGGGA 2 0.16515276630883566 No Hit TGCGGAGGGGTGATACCTAAAACAGGGTTTTACTGTAAGC 2 0.16515276630883566 No Hit TGCGGAGGGGGATTTGTTCACTGATTCCCATTATTTTCAG 2 0.16515276630883566 No Hit TGCGGAGGGATTCAAAAAAAAAAAAAAAAAAAAAAAAAAA 2 0.16515276630883566 No Hit TGCGGAGGGAGGAGAAGGCAAAACTGTGAATCTCAGCAGC 2 0.16515276630883566 No Hit TGCGGAGGGCAGTCTCTTGTCTCAGGCTCTCTGCCTTGTT 2 0.16515276630883566 No Hit TGCGGAGGGAGTGAAATTTAGCTCATTGAAATGTAAGTAT 2 0.16515276630883566 No Hit TGCGGAGGGAGAGCTAGTGAGGCTGGCTTTTTCCTCCAGG 2 0.16515276630883566 No Hit TGCGGAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGC 2 0.16515276630883566 No Hit TGCGGAGGGGGTAGAAAGTTTTTTCATAGAAGATGTATAA 2 0.16515276630883566 No Hit TGCGGAGGGACCTTTTAAGTTAGTGTCTTTTTGATATTCA 2 0.16515276630883566 No Hit TGCGGAGGGTAACAGATCCTCTTCTGAGATGAGTTTTTGT 2 0.16515276630883566 No Hit TGCGGAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2 0.16515276630883566 No Hit TGCGGAGGGAGTTATGGTTCCTTTGGTCGCTCGCTTCTCT 2 0.16515276630883566 No Hit TGCGGAGGGCATGGCAGTAAGCTTTGATGTTTGGTGGGAG 2 0.16515276630883566 No Hit TGCGGAGGGATTTCTAGCTTGTTGAAACCCAAAACTAATC 2 0.16515276630883566 No Hit TGCGGAGGGCTGCTAGTTGCTGATCAGCCAGTCAGTTCAT 2 0.16515276630883566 No Hit TGCGGAGGGGAGGTAGGAGGTCCTGGGTGTCTTTGGACGT 2 0.16515276630883566 No Hit TGCGGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2 0.16515276630883566 No Hit TGCGGAGGGGAATAGAAAATATATTCTGTTGAGTATTTAA 2 0.16515276630883566 No Hit TGCGGAGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACT 2 0.16515276630883566 No Hit TGCGGAGGGGCTAATGCCAGTTTAAAACTCCCAGGTTAAG 2 0.16515276630883566 No Hit TGCGGAGGGACTGTCAGATGGCCATATGTGCATTGTTCCT 2 0.16515276630883566 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.0 0.0 17 0.0 0.0 0.0 0.0 0.0 18 0.0 0.0 0.0 0.0 0.0 19 0.0 0.0 0.0 0.0 0.0 20 0.0 0.0 0.0 0.0 0.0 21 0.0 0.0 0.0 0.0 0.0 22 0.0 0.0 0.0 0.0 0.0 23 0.0 0.0 0.0 0.0 0.0 24 0.0 0.0 0.0 0.0 0.0 25 0.0 0.0 0.0 0.0 0.0 26 0.0 0.0 0.0 0.0 0.0 27 0.0 0.0 0.0 0.0 0.0 28 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGAGGG 110 0.0 34.0 3 AGGGTCA 30 5.9052636E-6 34.0 6 GCGGAGG 120 0.0 34.0 2 GGAGGGA 25 1.0617067E-4 34.0 4 TGCGGAG 120 0.0 34.0 1 ATCCTCT 20 0.0018237206 33.999996 16 GATCCTC 20 0.0018237206 33.999996 15 GGAGGGT 35 3.1267882E-7 33.999996 4 GAGGGTC 35 3.1267882E-7 33.999996 5 AGATCCT 20 0.0018237206 33.999996 14 GGAGGGG 50 2.0991138E-9 30.599998 4 >>END_MODULE