FastQCFastQC Report
Fri 17 Jun 2016
SRR1525930_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525930_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100719
Sequences flagged as poor quality0
Sequence length56
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATCAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG44274.395397094887757No Hit
TGATCAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG11131.1050546570160544No Hit
TGATCAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG5830.5788381536750762No Hit
TGATCAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG4610.4577090717739454No Hit
TGATCAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT4160.4130303120563151No Hit
TGATCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC3440.3415442965081067No Hit
TGATCAGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT3370.33459426721869756No Hit
TGATCAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG3190.31672276333164545No Hit
TGATCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC3170.31473704067752856No Hit
TGATCAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC2950.29289409148224266No Hit
TGATCAGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC2320.23034382787756033No Hit
TGATCAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCGTCTTCTGCTTGGATC2220.22041521460697583No Hit
TGATCAGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC2130.2114794626634498No Hit
TGATCAGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA1850.1836793455058132No Hit
TGATCAGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC1850.1836793455058132No Hit
TGATCAGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT1440.14297203109641676No Hit
TGATCAGGGGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCAT1430.14197916976935832No Hit
TGATCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC1410.13999344711524142No Hit
TGATCAGGGAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTG1230.12212194322818931No Hit
TGATCAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT1220.12112908190113086No Hit
TGATCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC1200.11914335924701398No Hit
TGATCAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC1180.11715763659289706No Hit
TGATCAGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC1070.10623616199525412No Hit
TGATCAGGGATCAGATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGG1050.10425043934113722No Hit
TGATCAGGGAACTGTGGTAATTCTAGAGCTAATACATGCCCACGGGCGCTGACCCC1010.10027899403290343No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTCA1550.050.5808941
CGCTCCG150.00803312950.58088741
CGATTGA150.008081431550.5042940
GTTTCAA150.00822763650.2758842
CCTAGCA150.00824400250.2506345
CATCTGG150.00824400250.2506343
TAGGTAC150.00826039250.22540334
CACGATT252.0252099E-550.20020347
TAGCAAG150.00827680550.20020347
AGATTAC301.0205094E-650.1247549
GATTACG252.0494008E-550.0996550
AGCCTCG204.1553949E-450.024511
GAGGTGT150.00840899349.99959
GGTCTCA150.00840899349.99959
GTGGCGC150.00855957349.77562320
TCCGTTG150.00855957349.77562317
AGGGATT1600.049.6767656
GGATATG150.00862713849.676768
GGAACGA150.00862713849.676768
GGGCAAC355.498805E-849.676767
Produced by FastQC (version 0.11.5)