FastQCFastQC Report
Fri 17 Jun 2016
SRR1525929_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525929_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4330
Sequences flagged as poor quality0
Sequence length40
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATCAGGGATGAGATGGACTTAAATGAATTGACGGGTAC340.7852193995381063No Hit
TGATCAGGGATGAGATGGCCTTAAATGAATTGACGGGTAC320.7390300230946882No Hit
TGATCAGGGATGAGATGGACTTAAAGGAATTGACGGGTAC300.6928406466512702No Hit
TGATCAGGGATGAGATGGACTCAAAGGAATTGACGGGTAC300.6928406466512702No Hit
TGATCAGGGATGAGATGGCCTTAAAGGAATTGACGGGTAC270.6235565819861432No Hit
TGATCAGGGAGTGCAGTGCTTGATCAGGGATGAGATGGAC220.5080831408775982No Hit
TGATCAGGGGAGTGCAGTGCTTGATCAGGGATGAGATGGA190.4387990762124711No Hit
TGATCAGGGATGAGATGGCCTCAAATGAATTGACGGGTAC180.41570438799076215No Hit
TGATCAGGGATGAGATGGACTCAAATGAATTGACGGGTAC160.3695150115473441No Hit
TGATCAGGGATGAGATGGACTTACAGGAATTGACGGGTAC110.2540415704387991No Hit
TGATCAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT90.20785219399538107No Hit
TGATCAGGGATGAGATGGCCTCAAAGGAATTGACGGGTAC90.20785219399538107No Hit
TGATCAGGGCTCGTCTTAATCAGCAACATCTTTTGATACA70.16166281755196305No Hit
TGATCAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT70.16166281755196305No Hit
TGATCAGGGATGAGATGGACTTACATGAATTGACGGGTAC60.13856812933025403No Hit
TGATCAGGGGGAAATCCTGTATTCCAAAAAGCAAGGTGGA60.13856812933025403No Hit
TGATCAGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.13856812933025403No Hit
TGATCAGGGATGAGATGGACTCACAGGAATTGACGGGTAC60.13856812933025403No Hit
TGATCAGGGGTGCAGTGCTTGATCAGGGATGAGATGGACT60.13856812933025403No Hit
TGATCAGGGATGAGATGGACTTAAATGAATTGACCGGTAC50.11547344110854503No Hit
TGATCAGGGATGAGATGGACTTAAATGACTTGACGGGTAC50.11547344110854503No Hit
TGATCAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG50.11547344110854503No Hit
TGATCAGGGATGAGATGGACTTAAAGGAATTGACCGGTAC50.11547344110854503No Hit
TGATCAGGGTAGAGATGTGGAGGAATACCGGGTACGCACA50.11547344110854503No Hit
TGATCAGGGGGGGTTGGGGATTTAGCTCCGTGGTAGAGCG50.11547344110854503No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACTCA251.6183403E-434.3176517
GGCCTTA251.6183403E-434.3176517
TGGACTC251.6183403E-434.3176516
GATGGCC404.908179E-834.31764614
GATGGAC357.3577394E-734.31764614
GCCTTAA200.002378254434.31764618
ATGGCCT404.908179E-834.31764615
ATGGACT357.3577394E-734.31764615
AGATGGC404.908179E-834.31764613
TGGCCTT301.094789E-534.31764616
TCAGGGC301.18965545E-533.9186064
GACGGGT502.4374458E-1033.91860632
ATGAATT453.6634447E-933.91860625
AGGGAAG358.095103E-733.9186066
CAGGGGA358.095103E-733.9186065
CAGGGAC301.18965545E-533.9186065
CAGGGAA453.6634447E-933.9186065
ACGGGTA502.4374458E-1033.91860633
TTGACGG601.8189894E-1233.91860630
GAATTGA551.6370905E-1133.91860627
Produced by FastQC (version 0.11.5)