##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1525929_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4330
Sequences flagged as poor quality	0
Sequence length	40
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.922401847575056	30.0	29.0	33.0	4.0	35.0
2	21.720785219399538	23.0	16.0	29.0	4.0	33.0
3	17.824018475750577	18.0	11.0	24.0	4.0	30.0
4	23.236027713625866	26.0	18.0	30.0	4.0	34.0
5	17.95958429561201	15.0	13.0	26.0	4.0	33.0
6	20.879676674364894	23.0	13.0	29.0	4.0	33.0
7	23.763972286374134	27.0	20.0	31.0	4.0	34.0
8	22.393533487297923	26.0	15.0	30.0	4.0	33.0
9	23.110854503464203	27.0	18.0	31.0	4.0	34.0
10	16.880600461893764	16.0	8.0	25.0	4.0	31.0
11	24.419399538106237	29.0	14.0	35.0	4.0	37.0
12	23.963972286374133	29.0	13.0	35.0	4.0	37.0
13	24.246420323325633	29.0	15.0	34.0	4.0	37.0
14	25.11524249422633	30.0	16.0	35.0	4.0	38.0
15	22.385219399538105	27.0	9.0	33.0	4.0	36.0
16	21.035796766743648	25.0	4.0	32.0	4.0	36.0
17	21.522863741339492	26.0	4.0	33.0	4.0	36.0
18	20.99353348729792	25.0	4.0	32.0	4.0	36.0
19	18.081986143187066	18.0	4.0	30.0	4.0	34.0
20	20.20323325635104	24.0	4.0	33.0	4.0	36.0
21	17.333025404157045	17.0	4.0	30.0	4.0	35.0
22	17.10415704387991	15.0	4.0	30.0	4.0	34.0
23	16.897921478060045	16.0	4.0	29.0	4.0	34.0
24	16.669745958429562	15.0	4.0	30.0	4.0	34.0
25	16.051963048498845	12.0	4.0	29.0	4.0	34.0
26	14.737644341801385	4.0	4.0	28.0	4.0	34.0
27	14.386836027713626	4.0	4.0	27.0	4.0	34.0
28	14.118937644341802	4.0	4.0	27.0	4.0	33.0
29	13.287066974595843	4.0	4.0	25.0	4.0	33.0
30	12.6729792147806	4.0	4.0	24.0	4.0	32.0
31	12.458198614318707	4.0	4.0	25.0	4.0	33.0
32	11.677136258660507	4.0	4.0	22.0	4.0	31.0
33	11.542263279445727	4.0	4.0	22.0	4.0	31.0
34	11.650346420323325	4.0	4.0	22.0	4.0	32.0
35	10.85473441108545	4.0	4.0	19.0	4.0	31.0
36	11.203233256351039	4.0	4.0	19.0	4.0	33.0
37	10.921709006928406	4.0	4.0	17.0	4.0	33.0
38	10.828637413394919	4.0	4.0	17.0	4.0	32.0
39	10.421016166281754	4.0	4.0	15.0	4.0	32.0
40	10.681062355658199	4.0	4.0	17.0	4.0	32.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	-23.158460317460314
1	2	-17.080841269841272
1	3	-13.18225
1	4	-18.24957142857143
1	5	-13.027837301587304
1	6	-16.009035714285712
1	7	-18.863809523809522
1	8	-17.536694444444446
1	9	-18.220341269841274
1	10	-12.646134920634921
1	11	-19.476384920634924
1	12	-18.38520634920635
1	13	-19.2543253968254
1	14	-20.048031746031747
1	15	-17.301210317460324
1	16	-16.11232142857143
1	17	-16.953980158730165
1	18	-15.999888888888893
1	19	-12.656603174603177
1	20	-15.08181746031746
1	21	-12.189301587301589
1	22	-11.706107142857139
1	23	-11.762182539682538
1	24	-11.348793650793652
1	25	-10.402404761904767
1	26	-8.811345238095237
1	27	-9.05890873015873
1	28	-8.616023809523814
1	29	-8.25764682539682
1	30	-7.788182539682541
1	31	-7.705873015873021
1	32	-6.5954126984127
1	33	-6.703956349206351
1	34	-6.596039682539683
1	35	-6.135242063492065
1	36	-6.18870238095238
1	37	-6.2642023809523835
1	38	-6.011238095238097
1	39	-5.492007936507939
1	40	-6.199547619047621
6	1	-0.28346031746031386
6	2	-0.08084126984127238
6	3	4.06775
6	4	-5.4995714285714286
6	5	-4.152837301587304
6	6	3.8659642857142877
6	7	3.261190476190478
6	8	-0.28669444444444636
6	9	4.154658730158726
6	10	2.353865079365079
6	11	0.1486150793650758
6	12	0.11479365079365067
6	13	5.995674603174599
6	14	3.7019682539682535
6	15	5.323789682539676
6	16	-1.7373214285714305
6	17	0.6710198412698354
6	18	5.5001111111111065
6	19	3.843396825396823
6	20	7.29318253968254
6	21	12.310698412698411
6	22	10.543892857142861
6	23	12.487817460317462
6	24	14.151206349206348
6	25	13.972595238095233
6	26	14.688654761904763
6	27	13.94109126984127
6	28	12.008976190476186
6	29	15.36735317460318
6	30	10.461817460317459
6	31	10.04412698412698
6	32	9.0295873015873
6	33	11.54604365079365
6	34	9.528960317460317
6	35	7.489757936507935
6	36	12.18629761904762
6	37	15.360797619047617
6	38	14.738761904761903
6	39	8.007992063492061
6	40	6.550452380952379
7	1	0.8415396825396861
7	2	4.808047619047617
7	3	7.595527777777779
7	4	7.083761904761904
7	5	-0.027837301587304353
7	6	6.32429761904762
7	7	-0.30825396825396467
7	8	1.1299722222222215
7	9	2.8907698412698366
7	10	8.687198412698411
7	11	8.745837301587297
7	12	10.725904761904765
7	13	9.96789682539682
7	14	7.507523809523811
7	15	3.4765674603174546
7	16	8.665456349206348
7	17	9.823797619047614
7	18	7.666777777777774
7	19	11.232285714285712
7	20	8.695960317460319
7	21	12.58847619047619
7	22	15.18278174603175
7	23	14.348928571428573
7	24	14.98453968253968
7	25	10.486484126984122
7	26	6.188654761904763
7	27	9.829980158730159
7	28	5.828420634920629
7	29	10.964575396825401
7	30	5.878484126984127
7	31	3.8496825396825347
7	32	0.29347619047618956
7	33	-0.037289682539684676
7	34	0.1817380952380958
7	35	0.30920238095237984
7	36	-0.0775912698412693
7	37	1.6246865079365058
7	38	0.3220952380952369
7	39	0.17465873015872724
7	40	-0.7551031746031764
8	1	1.0637619047619076
8	2	2.4747142857142848
8	3	2.484416666666668
8	4	-4.805126984126986
8	5	-3.138948412698415
8	6	5.990964285714288
8	7	0.2473015873015889
8	8	1.0188611111111108
8	9	0.8907698412698366
8	10	-5.979468253968255
8	11	-0.8097182539682564
8	12	0.9481269841269828
8	13	-1.476547619047622
8	14	-4.0480317460317465
8	15	-0.9678769841269919
8	16	2.109900793650791
8	17	-7.398424603174609
8	18	2.888999999999996
8	19	5.232285714285712
8	20	4.8070714285714295
8	21	8.921809523809522
8	22	4.293892857142861
8	23	8.460039682539684
8	24	10.206761904761905
8	25	-2.0690714285714336
8	26	5.410876984126984
8	27	3.3855357142857123
8	28	3.7173095238095186
8	29	3.186797619047624
8	30	2.767373015873014
8	31	3.071904761904758
8	32	3.737920634920634
8	33	3.8515992063492046
8	34	5.515071428571428
8	35	2.864757936507935
8	36	0.36685317460317535
8	37	2.0691309523809505
8	38	1.2109841269841244
8	39	1.6191031746031719
8	40	2.5782301587301575
9	1	1.3415396825396861
9	2	6.544158730158728
9	3	0.0677500000000002
9	4	7.1254285714285714
9	5	-2.2778373015873044
9	6	1.2409642857142877
9	7	8.011190476190478
9	8	8.213305555555554
9	9	7.154658730158726
9	10	9.103865079365079
9	11	5.648615079365076
9	12	4.739793650793651
9	13	4.870674603174599
9	14	-0.29803174603174654
9	15	5.573789682539676
9	16	10.88767857142857
9	17	11.671019841269835
9	18	13.500111111111107
9	19	10.218396825396823
9	20	13.79318253968254
9	21	17.56069841269841
9	22	12.043892857142861
9	23	17.487817460317462
9	24	19.651206349206348
9	25	15.472595238095233
9	26	17.438654761904765
9	27	15.56609126984127
9	28	14.383976190476186
9	29	11.74235317460318
9	30	15.086817460317459
9	31	9.16912698412698
9	32	12.4045873015873
9	33	8.17104365079365
9	34	12.403960317460317
9	35	12.489757936507935
9	36	14.31129761904762
9	37	13.985797619047617
9	38	17.7387619047619
9	39	9.507992063492061
9	40	11.300452380952379
10	1	2.841539682539686
10	2	-0.08084126984127238
10	3	-7.18225
10	4	6.7504285714285714
10	5	-2.0278373015873044
10	6	7.990964285714288
10	7	10.136190476190478
10	8	6.463305555555554
10	9	8.779658730158726
10	10	7.353865079365079
10	11	14.523615079365076
10	12	15.61479365079365
10	13	14.7456746031746
10	14	13.951968253968253
10	15	-4.301210317460324
10	16	4.8876785714285695
10	17	16.046019841269835
10	18	16.000111111111107
10	19	20.343396825396823
10	20	13.91818253968254
10	21	19.81069841269841
10	22	21.29389285714286
10	23	17.237817460317462
10	24	21.651206349206348
10	25	22.59759523809523
10	26	14.188654761904763
10	27	12.94109126984127
10	28	-1.6160238095238135
10	29	11.74235317460318
10	30	-1.7881825396825413
10	31	5.294126984126979
10	32	2.4045873015873003
10	33	18.29604365079365
10	34	18.403960317460317
10	35	7.864757936507935
10	36	7.81129761904762
10	37	20.735797619047617
10	38	23.9887619047619
10	39	24.50799206349206
10	40	26.80045238095238
11	1	2.5558253968254014
11	2	4.919158730158728
11	3	4.9606071428571425
11	4	-0.6781428571428556
11	5	3.2578769841269803
11	6	6.705250000000003
11	7	8.27904761904762
11	8	10.463305555555554
11	9	8.636801587301584
11	10	7.925293650793652
11	11	11.80932936507936
11	12	13.18622222222222
11	13	11.602817460317453
11	14	11.809111111111108
11	15	12.270218253968245
11	16	10.744821428571427
11	17	14.046019841269835
11	18	16.000111111111107
11	19	10.629111111111108
11	20	16.203896825396825
11	21	18.667841269841265
11	22	15.008178571428576
11	23	16.523531746031747
11	24	11.365492063492063
11	25	9.45473809523809
11	26	8.188654761904763
11	27	-0.4874801587301594
11	28	-0.4731666666666712
11	29	-0.971932539682534
11	30	0.3546746031746011
11	31	-3.277301587301592
11	32	-2.166841269841271
11	33	-2.1325277777777796
11	34	-2.0246111111111116
11	35	-1.706670634920636
11	36	-6.18870238095238
11	37	-6.2642023809523835
11	38	-6.011238095238097
11	39	-5.492007936507939
11	40	-6.199547619047621
12	1	-0.03346031746031386
12	2	1.2941587301587276
12	3	1.5677500000000002
12	4	0.7504285714285714
12	5	-2.5278373015873044
12	6	3.2409642857142877
12	7	4.386190476190478
12	8	7.338305555555554
12	9	4.904658730158726
12	10	5.228865079365079
12	11	13.273615079365076
12	12	13.73979365079365
12	13	13.2456746031746
12	14	12.326968253968253
12	15	11.573789682539676
12	16	15.63767857142857
12	17	14.921019841269835
12	18	12.500111111111107
12	19	5.843396825396823
12	20	13.79318253968254
12	21	12.560698412698411
12	22	11.793892857142861
12	23	11.862817460317462
12	24	15.776206349206348
12	25	17.47259523809523
12	26	5.313654761904763
12	27	5.9410912698412695
12	28	8.133976190476186
12	29	5.2423531746031795
12	30	5.836817460317459
12	31	3.1691269841269794
12	32	2.2795873015873003
12	33	0.5460436507936492
12	34	3.7789603174603172
12	35	1.4897579365079352
12	36	3.81129761904762
12	37	7.2357976190476165
12	38	1.988761904761903
12	39	5.007992063492061
12	40	2.300452380952379
13	1	-4.033460317460314
13	2	-10.330841269841272
13	3	-6.43225
13	4	-10.499571428571429
13	5	3.2221626984126956
13	6	-7.384035714285712
13	7	-4.988809523809522
13	8	-5.536694444444446
13	9	-6.095341269841274
13	10	-6.521134920634921
13	11	0.1486150793650758
13	12	1.3647936507936507
13	13	-0.004325396825400674
13	14	0.20196825396825346
13	15	1.698789682539676
13	16	0.7626785714285695
13	17	1.7960198412698354
13	18	1.7501111111111065
13	19	4.343396825396823
13	20	0.5431825396825403
13	21	3.560698412698411
13	22	2.793892857142861
13	23	0.8628174603174621
13	24	-0.09879365079365243
13	25	1.0975952380952325
13	26	1.688654761904763
13	27	0.8160912698412695
13	28	1.8839761904761865
13	29	-3.3826468253968205
13	30	-1.1631825396825413
13	31	-4.455873015873021
13	32	-6.5954126984127
13	33	-6.703956349206351
13	34	-6.596039682539683
13	35	-6.135242063492065
13	36	-6.18870238095238
13	37	-6.2642023809523835
13	38	-6.011238095238097
13	39	-5.492007936507939
13	40	-6.199547619047621
15	1	7.741539682539685
15	2	9.919158730158728
15	3	9.917750000000002
15	4	8.350428571428573
15	5	15.072162698412697
15	6	9.490964285714288
15	7	10.136190476190478
15	8	11.863305555555552
15	9	11.579658730158723
15	10	11.45386507936508
15	11	8.823615079365073
15	12	8.914793650793651
15	13	9.445674603174602
15	14	11.451968253968253
15	15	7.098789682539675
15	16	5.587678571428569
15	17	7.846019841269836
15	18	2.5001111111111065
15	19	5.843396825396823
15	20	7.018182539682542
15	21	9.810698412698411
15	22	8.99389285714286
15	23	12.337817460317464
15	24	11.951206349206348
15	25	11.597595238095233
15	26	17.188654761904765
15	27	13.141091269841269
15	28	9.783976190476185
15	29	12.442353174603179
15	30	7.5118174603174594
15	31	7.194126984126978
15	32	9.304587301587299
15	33	9.89604365079365
15	34	9.503960317460319
15	35	8.064757936507934
15	36	8.81129761904762
15	37	5.7357976190476165
15	38	5.888761904761903
15	39	7.807992063492062
15	40	9.500452380952378
16	1	1.008206349206354
16	2	4.25249206349206
16	3	1.984416666666668
16	4	6.2504285714285714
16	5	0.47216269841269565
16	6	0.6576309523809556
16	7	4.46952380952381
16	8	6.1299722222222215
16	9	5.112992063492058
16	10	5.020531746031747
16	11	10.023615079365076
16	12	11.448126984126986
16	13	9.579007936507935
16	14	8.78530158730159
16	15	10.032123015873008
16	16	8.054345238095237
16	17	8.046019841269835
16	18	6.833444444444439
16	19	3.510063492063491
16	20	5.751515873015872
16	21	-0.02263492063492123
16	22	-3.206107142857139
16	23	0.9044841269841299
16	24	2.3178730158730154
16	25	-5.069071428571434
16	26	-5.811345238095237
16	27	-5.392242063492064
16	28	-7.6160238095238135
16	29	-7.2576468253968205
16	30	-3.4548492063492073
16	31	-3.2058730158730206
16	32	-2.0954126984126997
16	33	-2.2039563492063508
16	34	-4.76270634920635
16	35	-1.6352420634920648
16	36	-1.68870238095238
16	37	-1.7642023809523835
16	38	-6.011238095238097
16	39	-5.492007936507939
16	40	-6.199547619047621
17	1	4.041539682539685
17	2	0.8191587301587262
17	3	-0.8822499999999991
17	4	6.2504285714285714
17	5	-0.32783730158730506
17	6	3.7909642857142885
17	7	4.8361904761904775
17	8	3.663305555555553
17	9	6.479658730158725
17	10	1.1538650793650795
17	11	8.023615079365076
17	12	8.414793650793651
17	13	8.0456746031746
17	14	9.25196825396825
17	15	7.398789682539675
17	16	6.68767857142857
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110	40	-6.199547619047621
111	1	-0.6584603174603139
111	2	2.9191587301587276
111	3	-4.68225
111	4	-1.7495714285714286
111	5	4.972162698412696
111	6	-0.00903571428571226
111	7	8.136190476190478
111	8	-1.0366944444444464
111	9	-1.7203412698412741
111	10	5.353865079365079
111	11	1.5236150793650758
111	12	-10.88520634920635
111	13	-5.754325396825401
111	14	-9.048031746031747
111	15	-8.801210317460324
111	16	-9.11232142857143
111	17	-7.453980158730165
111	18	-5.4998888888888935
111	19	-1.6566031746031769
111	20	-2.0818174603174597
111	21	-0.18930158730158908
111	22	1.2938928571428612
111	23	-1.262182539682538
111	24	2.6512063492063476
111	25	1.5975952380952325
111	26	-0.31134523809523706
111	27	-3.0589087301587305
111	28	3.8839761904761865
111	29	-4.2576468253968205
111	30	0.21181746031745874
111	31	-2.2058730158730206
111	32	1.9045873015873003
111	33	-0.20395634920635075
111	34	-3.5960396825396828
111	35	0.8647579365079352
111	36	7.31129761904762
111	37	-1.2642023809523835
111	38	7.488761904761903
111	39	-5.492007936507939
111	40	-6.199547619047621
112	1	7.841539682539686
112	2	9.919158730158728
112	3	6.31775
112	4	2.2504285714285714
112	5	-0.5278373015873044
112	6	6.490964285714288
112	7	8.136190476190478
112	8	-0.5366944444444464
112	9	1.7796587301587259
112	10	16.35386507936508
112	11	0.5236150793650758
112	12	5.114793650793651
112	13	-3.7543253968254007
112	14	6.4519682539682535
112	15	-9.301210317460324
112	16	-3.1123214285714305
112	17	0.04601984126983538
112	18	-9.499888888888893
112	19	-9.156603174603177
112	20	-8.58181746031746
112	21	-1.689301587301589
112	22	-6.206107142857139
112	23	-1.762182539682538
112	24	-5.848793650793652
112	25	5.0975952380952325
112	26	-4.311345238095237
112	27	-6.0589087301587305
112	28	-4.1160238095238135
112	29	2.7423531746031795
112	30	4.211817460317459
112	31	-4.705873015873021
112	32	5.9045873015873
112	33	1.2960436507936492
112	34	-2.0960396825396828
112	35	6.864757936507935
112	36	9.31129761904762
112	37	6.2357976190476165
112	38	9.488761904761903
112	39	7.007992063492061
112	40	7.800452380952379
113	1	2.841539682539686
113	2	-17.080841269841272
113	3	-13.18225
113	4	-18.24957142857143
113	5	-13.027837301587304
113	6	-16.009035714285712
113	7	-18.863809523809522
113	8	-17.536694444444446
113	9	-18.220341269841274
113	10	-12.646134920634921
113	11	-19.476384920634924
113	12	-18.38520634920635
113	13	-19.2543253968254
113	14	-20.048031746031747
113	15	-17.301210317460324
113	16	-16.11232142857143
113	17	-16.953980158730165
113	18	-15.999888888888893
113	19	-12.656603174603177
113	20	-15.08181746031746
113	21	-12.189301587301589
113	22	-11.706107142857139
113	23	-11.762182539682538
113	24	-11.348793650793652
113	25	-10.402404761904767
113	26	-8.811345238095237
113	27	-9.05890873015873
113	28	-8.616023809523814
113	29	-8.25764682539682
113	30	-7.788182539682541
113	31	-7.705873015873021
113	32	-6.5954126984127
113	33	-6.703956349206351
113	34	-6.596039682539683
113	35	-6.135242063492065
113	36	-6.18870238095238
113	37	-6.2642023809523835
113	38	-6.011238095238097
113	39	-5.492007936507939
113	40	-6.199547619047621
114	1	-1.6584603174603139
114	2	-1.5808412698412724
114	3	5.31775
114	4	1.2504285714285714
114	5	-4.027837301587304
114	6	3.4909642857142877
114	7	1.6361904761904782
114	8	4.963305555555554
114	9	2.279658730158726
114	10	-4.146134920634921
114	11	2.023615079365076
114	12	4.114793650793651
114	13	5.745674603174599
114	14	6.4519682539682535
114	15	2.198789682539676
114	16	-4.1123214285714305
114	17	-3.9539801587301646
114	18	7.0001111111111065
114	19	-3.156603174603177
114	20	-0.5818174603174597
114	21	-6.689301587301589
114	22	-3.706107142857139
114	23	1.737817460317462
114	24	-11.348793650793652
114	25	-10.402404761904767
114	26	-8.811345238095237
114	27	-9.05890873015873
114	28	-8.616023809523814
114	29	-8.25764682539682
114	30	-7.788182539682541
114	31	-7.705873015873021
114	32	-6.5954126984127
114	33	-6.703956349206351
114	34	-6.596039682539683
114	35	-6.135242063492065
114	36	-6.18870238095238
114	37	-6.2642023809523835
114	38	-6.011238095238097
114	39	-5.492007936507939
114	40	-6.199547619047621
117	1	-0.6584603174603139
117	2	4.919158730158728
117	3	6.31775
117	4	-7.2495714285714286
117	5	-6.027837301587304
117	6	-4.009035714285712
117	7	1.1361904761904782
117	8	-7.036694444444446
117	9	1.2796587301587259
117	10	6.853865079365079
117	11	6.023615079365076
117	12	-7.385206349206349
117	13	0.7456746031745993
117	14	3.9519682539682535
117	15	9.198789682539676
117	16	13.88767857142857
117	17	14.546019841269835
117	18	13.000111111111107
117	19	18.843396825396823
117	20	15.41818253968254
117	21	13.310698412698411
117	22	18.79389285714286
117	23	18.737817460317462
117	24	12.151206349206348
117	25	17.59759523809523
117	26	4.688654761904763
117	27	4.9410912698412695
117	28	4.8839761904761865
117	29	2.7423531746031795
117	30	4.211817460317459
117	31	4.294126984126979
117	32	3.9045873015873003
117	33	7.796043650793649
117	34	-6.596039682539683
117	35	-6.135242063492065
117	36	-6.18870238095238
117	37	-6.2642023809523835
117	38	-6.011238095238097
117	39	-5.492007936507939
117	40	-6.199547619047621
118	1	-8.658460317460314
118	2	-5.580841269841272
118	3	-10.18225
118	4	-5.2495714285714286
118	5	-2.5278373015873044
118	6	-12.509035714285712
118	7	-4.363809523809522
118	8	-3.0366944444444464
118	9	-3.720341269841274
118	10	-7.146134920634921
118	11	-2.476384920634924
118	12	-1.3852063492063493
118	13	-2.2543253968254007
118	14	-6.5480317460317465
118	15	-0.301210317460324
118	16	-1.1123214285714305
118	17	-1.9539801587301646
118	18	-1.9998888888888935
118	19	-3.656603174603177
118	20	-1.0818174603174597
118	21	4.310698412698411
118	22	0.7938928571428612
118	23	4.237817460317462
118	24	-11.348793650793652
118	25	-10.402404761904767
118	26	-8.811345238095237
118	27	-9.05890873015873
118	28	-8.616023809523814
118	29	-8.25764682539682
118	30	-7.788182539682541
118	31	-7.705873015873021
118	32	-6.5954126984127
118	33	-6.703956349206351
118	34	-6.596039682539683
118	35	-6.135242063492065
118	36	-6.18870238095238
118	37	-6.2642023809523835
118	38	-6.011238095238097
118	39	-5.492007936507939
118	40	-6.199547619047621
120	1	7.841539682539686
120	2	13.919158730158728
120	3	9.81775
120	4	12.750428571428571
120	5	-4.027837301587304
120	6	2.9909642857142877
120	7	-3.863809523809522
120	8	11.463305555555554
120	9	-18.220341269841274
120	10	-12.646134920634921
120	11	-19.476384920634924
120	12	-18.38520634920635
120	13	-19.2543253968254
120	14	-20.048031746031747
120	15	-17.301210317460324
120	16	-16.11232142857143
120	17	-16.953980158730165
120	18	-15.999888888888893
120	19	-12.656603174603177
120	20	-15.08181746031746
120	21	-12.189301587301589
120	22	-11.706107142857139
120	23	-11.762182539682538
120	24	-11.348793650793652
120	25	-10.402404761904767
120	26	-8.811345238095237
120	27	-9.05890873015873
120	28	-8.616023809523814
120	29	-8.25764682539682
120	30	-7.788182539682541
120	31	-7.705873015873021
120	32	-6.5954126984127
120	33	-6.703956349206351
120	34	-6.596039682539683
120	35	-6.135242063492065
120	36	-6.18870238095238
120	37	-6.2642023809523835
120	38	-6.011238095238097
120	39	-5.492007936507939
120	40	-6.199547619047621
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
4	705.0
5	12.0
6	60.0
7	35.0
8	31.0
9	59.0
10	69.0
11	120.0
12	113.0
13	167.0
14	162.0
15	174.0
16	180.0
17	175.0
18	188.0
19	184.0
20	157.0
21	178.0
22	211.0
23	194.0
24	222.0
25	206.0
26	192.0
27	158.0
28	145.0
29	89.0
30	67.0
31	41.0
32	22.0
33	7.0
34	6.0
35	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	100.0	0.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	0.0	100.0	0.0
5	0.0	0.0	0.0	100.0
6	0.0	100.0	0.0	0.0
7	99.35275080906149	0.53166897827092	0.09246417013407304	0.02311604253351826
8	97.85120147874306	0.7624768946395564	0.3927911275415897	0.9935304990757856
9	96.88005546568061	1.7333025190663278	0.5546568061012249	0.8319852091518374
10	31.64703164703165	45.899745899745895	9.494109494109495	12.959112959112959
11	22.540415704387993	27.89838337182448	33.25635103926097	16.304849884526558
12	48.775981524249424	15.173210161662817	18.40646651270208	17.644341801385682
13	23.90300230946882	31.224018475750576	24.133949191685915	20.73903002309469
14	38.31408775981524	22.702078521939953	20.785219399538107	18.198614318706696
15	21.755196304849886	37.99076212471132	20.669745958429562	19.584295612009235
16	21.20092378752887	19.053117782909933	40.438799076212476	19.30715935334873
17	38.86836027713626	19.838337182448036	20.0	21.293302540415702
18	36.69745958429561	21.570438799076214	20.854503464203233	20.87759815242494
19	19.939717134245306	20.843960120565733	13.702759100394157	45.51356364479481
20	21.454965357967666	19.907621247113163	21.801385681293304	36.83602771362587
21	22.07852193995381	18.752886836027713	37.06697459584296	22.10161662817552
22	20.27713625866051	19.006928406466514	29.769053117782914	30.94688221709007
23	24.43418013856813	32.77136258660508	22.79445727482679	20.0
24	17.25970425138632	34.542513863216264	21.626617375231053	26.571164510166355
25	20.74468085106383	34.505087881591116	20.4209065679926	24.32932469935245
26	23.808416647291327	23.134154847709834	28.179493141129967	24.87793536386887
27	31.9011319011319	20.62832062832063	24.347424347424347	23.123123123123122
28	20.836220836220836	31.762531762531765	25.271425271425272	22.12982212982213
29	18.783252371038632	31.182049502660192	25.931066389081657	24.103631737219526
30	17.083333333333332	19.282407407407405	43.00925925925926	20.625
31	15.25462962962963	21.48148148148148	45.6712962962963	17.59259259259259
32	30.831408775981522	16.02771362586605	33.23325635103926	19.907621247113163
33	15.05773672055427	30.27713625866051	34.45727482678984	20.207852193995382
34	14.295612009237876	20.96997690531178	36.489607390300236	28.244803695150118
35	26.512702078521936	19.145496535796767	36.05080831408776	18.290993071593533
36	25.86605080831409	20.531177829099306	36.581986143187066	17.020785219399535
37	23.596024959556274	19.320545412526	38.96464062861105	18.11878899930668
38	15.334872979214781	19.81524249422633	46.65127020785219	18.198614318706696
39	14.157043879907622	29.399538106235568	35.01154734411085	21.43187066974596
40	11.339491916859123	23.48729792147806	34.89607390300231	30.27713625866051
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	1.5
12	3.0
13	3.0
14	7.0
15	11.0
16	16.0
17	21.0
18	21.0
19	23.5
20	26.0
21	29.5
22	33.0
23	33.0
24	42.0
25	51.0
26	60.0
27	69.0
28	69.0
29	90.0
30	111.0
31	105.5
32	100.0
33	100.0
34	153.5
35	207.0
36	221.0
37	235.0
38	235.0
39	247.5
40	260.0
41	281.5
42	303.0
43	303.0
44	349.5
45	396.0
46	420.0
47	444.0
48	444.0
49	378.0
50	312.0
51	342.0
52	372.0
53	372.0
54	350.5
55	329.0
56	313.0
57	297.0
58	297.0
59	263.5
60	230.0
61	191.5
62	153.0
63	153.0
64	145.0
65	137.0
66	117.5
67	98.0
68	98.0
69	79.0
70	60.0
71	50.0
72	40.0
73	40.0
74	31.0
75	22.0
76	13.5
77	5.0
78	5.0
79	3.5
80	2.0
81	2.0
82	2.0
83	2.0
84	1.5
85	1.0
86	0.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.09237875288683603
8	0.046189376443418015
9	0.06928406466512702
10	0.023094688221709007
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.39260969976905313
20	0.0
21	0.0
22	0.0
23	0.0
24	0.046189376443418015
25	0.13856812933025403
26	0.6697459584295612
27	0.023094688221709007
28	0.023094688221709007
29	0.16166281755196305
30	0.23094688221709006
31	0.23094688221709006
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.06928406466512702
38	0.0
39	0.0
40	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
40	4330.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.80600461893765
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.95002630194634	83.37182448036951
2	3.340347185691741	5.866050808314087
3	0.6575486586007364	1.7321016166281753
4	0.3945291951604419	1.3856812933025404
5	0.15781167806417673	0.6928406466512702
6	0.1315097317201473	0.6928406466512702
7	0.052603892688058915	0.3233256351039261
8	0.0	0.0
9	0.052603892688058915	0.41570438799076215
>10	0.2630194634402946	5.519630484988452
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TGATCAGGGATGAGATGGACTTAAATGAATTGACGGGTAC	34	0.7852193995381063	No Hit
TGATCAGGGATGAGATGGCCTTAAATGAATTGACGGGTAC	32	0.7390300230946882	No Hit
TGATCAGGGATGAGATGGACTTAAAGGAATTGACGGGTAC	30	0.6928406466512702	No Hit
TGATCAGGGATGAGATGGACTCAAAGGAATTGACGGGTAC	30	0.6928406466512702	No Hit
TGATCAGGGATGAGATGGCCTTAAAGGAATTGACGGGTAC	27	0.6235565819861432	No Hit
TGATCAGGGAGTGCAGTGCTTGATCAGGGATGAGATGGAC	22	0.5080831408775982	No Hit
TGATCAGGGGAGTGCAGTGCTTGATCAGGGATGAGATGGA	19	0.4387990762124711	No Hit
TGATCAGGGATGAGATGGCCTCAAATGAATTGACGGGTAC	18	0.41570438799076215	No Hit
TGATCAGGGATGAGATGGACTCAAATGAATTGACGGGTAC	16	0.3695150115473441	No Hit
TGATCAGGGATGAGATGGACTTACAGGAATTGACGGGTAC	11	0.2540415704387991	No Hit
TGATCAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT	9	0.20785219399538107	No Hit
TGATCAGGGATGAGATGGCCTCAAAGGAATTGACGGGTAC	9	0.20785219399538107	No Hit
TGATCAGGGCTCGTCTTAATCAGCAACATCTTTTGATACA	7	0.16166281755196305	No Hit
TGATCAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT	7	0.16166281755196305	No Hit
TGATCAGGGATGAGATGGACTTACATGAATTGACGGGTAC	6	0.13856812933025403	No Hit
TGATCAGGGGGAAATCCTGTATTCCAAAAAGCAAGGTGGA	6	0.13856812933025403	No Hit
TGATCAGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6	0.13856812933025403	No Hit
TGATCAGGGATGAGATGGACTCACAGGAATTGACGGGTAC	6	0.13856812933025403	No Hit
TGATCAGGGGTGCAGTGCTTGATCAGGGATGAGATGGACT	6	0.13856812933025403	No Hit
TGATCAGGGATGAGATGGACTTAAATGAATTGACCGGTAC	5	0.11547344110854503	No Hit
TGATCAGGGATGAGATGGACTTAAATGACTTGACGGGTAC	5	0.11547344110854503	No Hit
TGATCAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG	5	0.11547344110854503	No Hit
TGATCAGGGATGAGATGGACTTAAAGGAATTGACCGGTAC	5	0.11547344110854503	No Hit
TGATCAGGGTAGAGATGTGGAGGAATACCGGGTACGCACA	5	0.11547344110854503	No Hit
TGATCAGGGGGGGTTGGGGATTTAGCTCCGTGGTAGAGCG	5	0.11547344110854503	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.023094688221709007	0.0	0.0	0.0	0.0
15	0.023094688221709007	0.0	0.0	0.0	0.0
16	0.023094688221709007	0.0	0.0	0.0	0.0
17	0.023094688221709007	0.0	0.0	0.0	0.0
18	0.023094688221709007	0.0	0.0	0.0	0.0
19	0.023094688221709007	0.0	0.0	0.0	0.0
20	0.046189376443418015	0.0	0.0	0.0	0.0
21	0.046189376443418015	0.0	0.0	0.0	0.0
22	0.046189376443418015	0.0	0.0	0.0	0.0
23	0.046189376443418015	0.0	0.0	0.0	0.0
24	0.046189376443418015	0.0	0.0	0.0	0.0
25	0.046189376443418015	0.0	0.0	0.0	0.0
26	0.06928406466512702	0.0	0.0	0.0	0.0
27	0.06928406466512702	0.0	0.0	0.0	0.0
28	0.06928406466512702	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGACTCA	25	1.6183403E-4	34.31765	17
GGCCTTA	25	1.6183403E-4	34.31765	17
TGGACTC	25	1.6183403E-4	34.31765	16
GATGGCC	40	4.908179E-8	34.317646	14
GATGGAC	35	7.3577394E-7	34.317646	14
GCCTTAA	20	0.0023782544	34.317646	18
ATGGCCT	40	4.908179E-8	34.317646	15
ATGGACT	35	7.3577394E-7	34.317646	15
AGATGGC	40	4.908179E-8	34.317646	13
TGGCCTT	30	1.094789E-5	34.317646	16
TCAGGGC	30	1.18965545E-5	33.918606	4
GACGGGT	50	2.4374458E-10	33.918606	32
ATGAATT	45	3.6634447E-9	33.918606	25
AGGGAAG	35	8.095103E-7	33.918606	6
CAGGGGA	35	8.095103E-7	33.918606	5
CAGGGAC	30	1.18965545E-5	33.918606	5
CAGGGAA	45	3.6634447E-9	33.918606	5
ACGGGTA	50	2.4374458E-10	33.918606	33
TTGACGG	60	1.8189894E-12	33.918606	30
GAATTGA	55	1.6370905E-11	33.918606	27
>>END_MODULE