FastQCFastQC Report
Fri 17 Jun 2016
SRR1525927_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525927_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4314
Sequences flagged as poor quality0
Sequence length40
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATCAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT2114.8910523875753364No Hit
TGATCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC400.927213722763097No Hit
TGATCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG330.764951321279555No Hit
TGATCAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT230.5331478905887808No Hit
TGATCAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCC190.440426518312471No Hit
TGATCAGGGTACCTGGTTGATACTGCCAGTAGCATATGCT150.34770514603616137No Hit
TGATCAGGGTAACTGGTTGATCCTGCCAGTAGCATATGCT130.3013444598980065No Hit
TGATCAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG120.27816411682892905No Hit
TGATCAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.27816411682892905No Hit
TGATCAGGGTACCTGGTTGATCCTGCCAGTACCATATGCT90.2086230876216968No Hit
TGATCAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGA90.2086230876216968No Hit
TGATCAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCA90.2086230876216968No Hit
TGATCAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGC80.18544274455261936No Hit
TGATCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAG80.18544274455261936No Hit
TGATCAGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT70.16226240148354196No Hit
TGATCAGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAAC60.13908205841446453No Hit
TGATCAGGGAGTGAAACTGCGAATGGCTCATTAAATCAGT60.13908205841446453No Hit
TGATCAGGGGGGTGACGGGGAATCAGGGTTCGATTCCGGA60.13908205841446453No Hit
TGATCAGGGGGGTTGGAGAGATGGCTCAGTGGTTAAGAGC50.11590171534538712No Hit
TGATCAGGGCTCCTACTTGGATAACTGTGGTAATTCTAGA50.11590171534538712No Hit
TGATCAGGGCGGGGAGGTAGTGACGAAAAATAACAATACA50.11590171534538712No Hit
TGATCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGA50.11590171534538712No Hit
TGATCAGGGAACCTGAGTTTGGAAACTTCAGATAAGATTG50.11590171534538712No Hit
TGATCAGGGGGGGATGGTGAGATGGCTCAGTGGGTAAGAG50.11590171534538712No Hit
TGATCAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.11590171534538712No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGT251.6183403E-434.317656
TCAGGGG1850.034.317654
AGGGGCT200.002378254434.3176466
GTACCTG551.4551915E-1134.3176469
TCAGGGC301.094789E-534.3176464
GGGTACC551.4551915E-1134.3176467
AGGGTAC600.034.3176466
GGGGGGG301.094789E-534.3176467
CAGGGGT200.002378254434.3176465
CAGGGGG1050.034.3176465
CAGGGGC200.002378254434.3176465
CAGGGGA404.908179E-834.3176465
CAGGGAT301.094789E-534.3176465
GGTACCT551.4551915E-1134.3176468
GTTGATC551.6370905E-1133.91860616
TGGTTGA551.6370905E-1133.91860614
GGTTGAT551.6370905E-1133.91860615
CCAGTAG502.4374458E-1033.91860626
AGTAGCA502.4374458E-1033.91860628
CCTGCCA601.8189894E-1233.91860622
Produced by FastQC (version 0.11.5)