SRR1525926_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1525926_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	55362
Sequences flagged as poor quality	0
Sequence length	56
%GC	55

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GCGTCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	2795	5.048589285069181	No Hit
GCGTCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG	713	1.2878869983020844	No Hit
GCGTCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG	386	0.697229146345869	No Hit
GCGTCCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT	364	0.6574906975904049	No Hit
GCGTCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG	350	0.6322025938369278	No Hit
GCGTCCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC	348	0.6285900075864311	No Hit
GCGTCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG	226	0.4082222463061305	No Hit
GCGTCCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC	127	0.22939922690654238	No Hit
GCGTCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	114	0.20591741627831364	No Hit
GCGTCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	112	0.2023048300278169	No Hit
GCGTCCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC	111	0.20049853690256852	No Hit
GCGTCCGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATG	97	0.17521043314909143	No Hit
GCGTCCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA	96	0.17340414002384308	No Hit
GCGTCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCGTCTTCTGCTTGGATC	96	0.17340414002384308	No Hit
GCGTCCGGGGCCTGGAGGCCCATCTGGTACAGCTGGCAGATGCCTGGGACTGTAAG	90	0.16256638127235287	No Hit
GCGTCCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT	80	0.14450345001986922	No Hit
GCGTCCGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT	73	0.13185939814313066	No Hit
GCGTCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA	66	0.11921534626639212	No Hit
GCGTCCGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC	66	0.11921534626639212	No Hit
GCGTCCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	61	0.11018388064015028	No Hit
GCGTCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG	61	0.11018388064015028	No Hit
GCGTCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	59	0.10657129438965354	No Hit
GCGTCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTACG	57	0.10295870813915682	No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	57	0.10295870813915682	No Hit
GCGTCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCCGTCTCAAAGATTAAG	56	0.10115241501390845	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCAATCC	20	3.9040158E-4	50.619484	41
ACGCCTG	15	0.008018794	50.573	40
GCGCATT	15	0.008018794	50.573	38
CATGGGT	15	0.008018794	50.573	40
CGCATTG	15	0.008018794	50.573	39
TGCCCGT	25	1.9372983E-5	50.526608	37
GGCGCAT	15	0.008047978	50.526604	37
TGCTCGT	75	0.0	50.526604	37
ATGCCCG	20	3.9575202E-4	50.48029	36
TATGCTC	85	0.0	50.387924	35
TATGCTG	15	0.008136008	50.38792	35
TATGCCC	30	9.760606E-7	50.38792	35
ATTGCTT	20	4.0116056E-4	50.341866	34
ATATGCC	100	0.0	50.341866	34
CAAGATT	45	1.2551027E-10	50.204193	47
CAAAGAC	15	0.008254493	50.204193	47
TCCACGA	25	2.0231373E-5	50.15847	45
ACGATTA	35	5.03951E-8	50.15847	48
AGCAAGC	15	0.008284314	50.15847	48
GCCCAAA	20	4.0846315E-4	50.158466	48

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content