##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1525926_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 55362 Sequences flagged as poor quality 0 Sequence length 56 %GC 55 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.54627722986886 39.0 38.0 40.0 35.0 40.0 2 37.55644666016401 39.0 38.0 40.0 35.0 40.0 3 37.58280047686139 39.0 38.0 39.0 35.0 40.0 4 37.69504353166432 39.0 38.0 39.0 35.0 40.0 5 37.49465337234926 39.0 38.0 39.0 35.0 40.0 6 37.189931722119866 39.0 38.0 39.0 33.0 40.0 7 36.480636537697336 38.0 36.0 39.0 31.0 40.0 8 36.533976373685924 38.0 36.0 39.0 31.0 40.0 9 36.610075503052634 39.0 37.0 39.0 31.0 40.0 10 36.307105957154725 39.0 36.0 39.0 31.0 40.0 11 34.955113615837575 38.0 35.0 39.0 26.0 40.0 12 34.17172428741736 38.0 33.0 39.0 23.0 40.0 13 33.65541346049637 38.0 32.0 39.0 20.0 40.0 14 34.115096997940825 38.0 33.0 39.0 23.0 40.0 15 32.94134966222319 38.0 31.0 39.0 18.0 40.0 16 32.684783786712906 37.0 30.0 39.0 19.0 40.0 17 31.74415664173982 36.0 28.0 39.0 15.0 40.0 18 31.290777067302482 36.0 27.0 39.0 15.0 40.0 19 32.1850908565442 36.0 29.0 38.0 17.0 40.0 20 32.68897438676348 37.0 30.0 39.0 19.0 40.0 21 33.01358332430187 38.0 31.0 39.0 18.0 40.0 22 32.3472959791915 37.0 30.0 39.0 17.0 40.0 23 33.06544199992775 38.0 31.0 39.0 19.0 40.0 24 32.38428886239659 37.0 30.0 39.0 17.0 40.0 25 32.81462013655576 37.0 31.0 39.0 18.0 40.0 26 33.16982767963585 38.0 32.0 39.0 18.0 40.0 27 33.487012752429465 38.0 33.0 39.0 20.0 40.0 28 33.18962465228857 38.0 32.0 39.0 19.0 40.0 29 32.818467540912536 37.0 31.0 39.0 17.0 40.0 30 32.51354719843936 37.0 31.0 39.0 17.0 40.0 31 33.12681984032369 38.0 33.0 39.0 17.0 40.0 32 33.078013800079475 38.0 33.0 39.0 18.0 40.0 33 32.81503558397457 37.0 32.0 39.0 17.0 40.0 34 32.62268342906687 37.0 32.0 39.0 17.0 40.0 35 32.65788808207796 37.0 32.0 39.0 17.0 40.0 36 32.17295256674253 36.0 31.0 38.0 11.0 40.0 37 31.896463278060764 36.0 31.0 38.0 10.0 40.0 38 31.22631046566237 36.0 30.0 38.0 4.0 40.0 39 30.154221307033705 35.0 27.0 38.0 4.0 40.0 40 28.41788591452621 34.0 21.0 38.0 4.0 39.0 41 26.31431306672447 31.0 16.0 38.0 4.0 39.0 42 27.215219825873344 32.0 20.0 38.0 4.0 39.0 43 27.357086087930348 33.0 20.0 38.0 4.0 39.0 44 26.415573859325892 32.0 17.0 38.0 4.0 39.0 45 27.18994978505112 33.0 20.0 38.0 4.0 39.0 46 25.3017954553665 30.0 15.0 36.0 4.0 39.0 47 24.472598533289982 29.0 10.0 36.0 4.0 39.0 48 25.11623496260973 30.0 13.0 36.0 4.0 39.0 49 24.123893645460786 29.0 9.0 36.0 4.0 39.0 50 24.66393916404754 30.0 10.0 36.0 4.0 39.0 51 24.938206712185252 31.0 4.0 36.0 4.0 39.0 52 24.79921245619739 31.0 4.0 36.0 4.0 39.0 53 24.547957082475342 30.0 4.0 36.0 4.0 39.0 54 24.31579422708717 30.0 4.0 36.0 4.0 39.0 55 24.068458509446913 30.0 4.0 36.0 4.0 39.0 56 23.012174415664173 29.0 4.0 36.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 1.9331068221275487 1 2 1.8817211335558994 1 3 1.5570544309543166 1 4 1.4637305985666984 1 5 2.112344707346999 1 6 2.6073458528443183 1 7 2.8342182433782384 1 8 3.2686959824491737 1 9 3.4009207023956876 1 10 3.4279148772942634 1 11 1.0266744428759864 1 12 -6.563437309335786 1 13 -19.73315324613872 1 14 -5.511952115214907 1 15 1.316751376054853 1 16 -0.9638649635741103 1 17 -4.487171377408945 1 18 -3.6723147193019976 1 19 0.2841418764611312 1 20 -0.19207210738761304 1 21 7.106868788442245 1 22 8.342159897062597 1 23 7.550446507731024 1 24 8.074373532886884 1 25 7.408526842786621 1 26 6.941024191619238 1 27 6.7010390010753085 1 28 6.971308816432998 1 29 6.806400591516017 1 30 7.461480030828849 1 31 6.005269480019383 1 32 5.321129998111033 1 33 6.394763368112326 1 34 5.409075819491978 1 35 6.578956543611955 1 36 4.963043727385379 1 37 5.215267081757904 1 38 5.444564270977757 1 39 5.46901972121762 1 40 -10.319424593569732 1 41 2.893723853758157 1 42 0.8324714764383643 1 43 -6.725651399994945 1 44 -15.315151324953996 1 45 0.17744058736764856 1 46 -5.886941267959525 1 47 -11.303525153814187 1 48 -4.9384642255869835 1 49 -14.727873259872183 1 50 -3.636949559683643 1 51 1.3005915024727877 1 52 1.9321503587885616 1 53 1.8074125286029457 1 54 1.5688669040696972 1 55 1.2958813546660402 1 56 6.130218703036487 2 1 1.5614140767505944 2 2 0.7793029258745321 2 3 1.094892268792158 2 4 0.6648685786520474 2 5 1.2715196717851214 2 6 2.226691514295247 2 7 2.709751671543252 2 8 2.74920807348758 2 9 3.0501383410870133 2 10 2.6136901262274037 2 11 2.944313134198893 2 12 -0.8430959153101831 2 13 -0.3779612119993203 2 14 3.8140791792374387 2 15 4.345912115741903 2 16 4.236846273979236 2 17 6.031178551467303 2 18 3.9113268169711155 2 19 5.903629785422726 2 20 5.110346100293754 2 21 6.420524549466425 2 22 7.294080238456626 2 23 5.8648135063085505 2 24 6.368968127481484 2 25 5.934273642217633 2 26 6.436899013809846 2 27 5.299332030947291 2 28 5.7186772374856325 2 29 6.090753365342472 2 30 6.215818579904241 2 31 4.363021969350822 2 32 4.813021890002929 2 33 5.296897080772357 2 34 4.244922192180457 2 35 4.715798648875111 2 36 5.332460512591641 2 37 4.86420022542789 2 38 5.521377926738779 2 39 5.392206065456598 2 40 3.673747525918177 2 41 8.025160553615908 2 42 6.444562514845192 2 43 5.897961686776039 2 44 -3.08001618981886 2 45 6.347853105148587 2 46 5.387880922652137 2 47 1.1666028689454144 2 48 4.9074817203589625 2 49 -1.2257395472121537 2 50 5.205724693516924 2 51 6.976836168191138 2 52 6.6179256077217055 2 53 7.16929019574377 2 54 7.024912423273111 2 55 6.933150202461206 2 56 7.857103482552844 3 1 1.8976713866921173 3 2 1.900940352775116 3 3 1.5615589354588195 3 4 1.118084952921052 3 5 2.1498822448845303 3 6 2.7091476546461237 3 7 2.7822662914262892 3 8 3.005933219686412 3 9 3.1600798615548484 3 10 2.7318187811981645 3 11 4.541389157590707 3 12 4.76509121919274 3 13 5.4641440511585735 3 14 4.888348185085391 3 15 1.0248594841629597 3 16 6.840939841230696 3 17 7.2323481421105775 3 18 7.850508103520827 3 19 7.313571305890559 3 20 6.955375340059831 3 21 7.314676596250056 3 22 7.7595773144800155 3 23 7.551948009232525 3 24 6.613412571925924 3 25 7.331349665609444 3 26 6.639822990418036 3 27 6.0806185806548925 3 28 6.060197705321887 3 29 7.1229171080325315 3 30 6.693011562360379 3 31 5.933197407947315 3 32 6.001910778891819 3 33 5.786955560304516 3 34 5.137904648320809 3 35 4.845623210278621 3 36 7.034214898556552 3 37 6.374726541217367 3 38 7.8745943010077895 3 39 8.083434135632032 3 40 10.176671502526364 3 41 12.410540670574974 3 42 10.980819824786714 3 43 11.416090341746795 3 44 11.242206032403363 3 45 11.39756070748777 3 46 12.067413086394826 3 47 12.505784155495121 3 48 11.563037275914521 3 49 11.753207821208896 3 50 11.240227617493531 3 51 10.720111021992306 3 52 10.558276484914686 3 53 11.3587638799543 3 54 10.920818856021647 3 55 10.412097570882256 3 56 11.018506991324774 4 1 1.870146134166859 4 2 1.6830110598458248 4 3 1.5198922687921552 4 4 1.0706102054463074 4 5 1.726902446904738 4 6 2.287935533434002 4 7 2.4453976045576056 4 8 2.8077008964540866 4 9 3.284827336302328 4 10 3.2964652458446295 4 11 5.092399258600807 4 12 5.784535663637186 4 13 5.848234960249481 4 14 5.8590552557924624 4 15 6.1294049387084115 4 16 7.529071154362011 4 17 8.457853192615627 4 18 9.001518204530928 4 19 7.790591507910765 4 20 7.5975470572315515 4 21 7.071242252815711 4 22 8.065132870035573 4 23 7.382756090040601 4 24 7.350786309299661 4 25 7.161905221165 4 26 6.911540162135207 4 27 6.4043559543922655 4 28 6.425854270978451 4 29 6.382260542375967 4 30 6.564981259330079 4 31 6.270318620068526 4 32 5.940294617275654 4 33 6.021298994647953 4 34 5.866692527108686 4 35 5.726683816339229 4 36 6.2667906561323115 4 37 6.2088174503082705 4 38 7.20439228080577 4 39 8.129646256844154 4 40 -0.04024768939282808 4 41 10.98831844835275 4 42 10.007840026806917 4 43 10.2421004427569 4 44 10.444983810181139 4 45 9.56902535395242 4 46 12.106301975283717 4 47 12.084066983777952 4 48 12.189299902177144 4 49 12.546389639390718 4 50 12.348308425574345 4 51 11.586525163406453 4 52 12.493882545520748 4 53 11.783763879954297 4 54 11.88950572470852 4 55 11.409824843609531 4 56 12.354113051930835 5 1 1.568552612745755 5 2 1.0473005060318172 5 3 1.1592600848841101 5 4 0.8793876349133853 5 5 1.490878413466909 5 6 1.8804120224622167 5 7 2.581500007901383 5 8 2.8545922235178267 5 9 3.1654438462291807 5 10 2.8184088195123422 5 11 4.130278046479596 5 12 3.696891985476263 5 13 4.926213016675813 5 14 4.596777303859341 5 15 5.901870978415829 5 16 6.770058615177053 5 17 7.12047074747456 5 18 7.999167107352246 5 19 6.554567474472936 5 20 6.125107140826117 5 21 6.180960121154268 5 22 6.533523674633276 5 23 5.484898200803407 5 24 6.444830196446997 5 25 6.292652347601781 5 26 5.218750193483171 5 27 4.456863791382858 5 28 4.350619161260585 5 29 6.010656571634065 5 30 5.470023056613254 5 31 4.824002005648463 5 32 4.419918441727063 5 33 4.63676398942329 5 34 5.065490855217359 5 35 4.222634704531497 5 36 5.197433289361147 5 37 5.467829989493229 5 38 5.397965948517367 5 39 7.090330687356172 5 40 8.19812744122368 5 41 11.430847183984937 5 42 10.021815993369088 5 43 10.41072635707247 5 44 11.29699530443401 5 45 9.08300132051459 5 46 10.436761745398659 5 47 11.07283396392424 5 48 10.300585168634829 5 49 10.950908970634188 5 50 10.524901946995449 5 51 9.092141673333305 5 52 9.546782232041124 5 53 9.411637443172687 5 54 8.95376904759253 5 55 9.466120559388006 5 56 9.246093198221327 6 1 1.9109803000010288 6 2 1.6711479229826836 6 3 1.6641230380229217 6 4 1.1685123033484004 6 5 2.074180169182455 6 6 2.764459009957477 6 7 2.9121808213408187 6 8 3.2666169803701735 6 9 3.473388774863764 6 10 2.689572138951526 6 11 4.9288128450143915 6 12 5.7611840152855365 6 13 6.624583611598137 6 14 6.072841469578677 6 15 6.8809034402069145 6 16 7.4093159096067644 6 17 8.876059985822419 6 18 9.53573398874671 6 19 8.060946153265409 6 20 7.172347257031753 6 21 7.207106388679843 6 22 7.556891111793814 6 23 7.617271574556092 6 24 7.1360010945144445 6 25 6.5781140123737885 6 26 6.063388313983356 6 27 6.044965345001657 6 28 5.224056069180254 6 29 6.612904898020318 6 30 6.090081159429975 6 31 5.737226711976614 6 32 5.828406505387541 6 33 5.321998295347253 6 34 5.362446772862928 6 35 6.348187312842722 6 36 7.147035411377065 6 37 6.235166101656926 6 38 7.198648025061509 6 39 8.583067835265737 6 40 4.111225837080699 6 41 11.850955810990115 6 42 11.264458408425298 6 43 10.87933820499466 6 44 10.836467326664657 6 45 10.963006372933435 6 46 12.367290986272732 6 47 12.281119930830897 6 48 11.683305896183139 6 49 11.91364738164846 6 50 12.764891842157759 6 51 10.740745942627232 6 52 11.199302125940328 6 53 11.906016627207041 6 54 11.276618611821405 6 55 10.874734933519623 6 56 12.356235929053714 7 1 1.987903376924102 7 2 1.9678512196859828 7 3 1.5432439171438048 7 4 1.5641166989527946 7 5 2.228026323028608 7 6 2.2150084605069296 7 7 2.5934995026594976 7 8 2.9259576397108304 7 9 2.879982181457173 7 10 3.403857853237241 7 11 5.115626031827581 7 12 5.574370828472354 7 13 6.130078117092641 7 14 5.9189953157325235 7 15 6.298485857789331 7 16 7.1895356898265455 7 17 8.271664381426813 7 18 8.304964757977483 7 19 7.258748351067606 7 20 6.875643960328453 7 21 6.800512982086442 7 22 7.216231771134471 7 23 6.606282563567078 7 24 6.762374720888072 7 25 6.534157968417745 7 26 5.843608094203137 7 27 5.176833476869788 7 28 5.311968157092341 7 29 4.8436741287895515 7 30 5.7713998407486535 7 31 5.506457481207388 7 32 5.2569779339589715 7 33 4.838481811830771 7 34 4.9668423772585335 7 35 5.161374126029539 7 36 6.938244202585853 7 37 6.762638629129452 7 38 7.319527145940633 7 39 7.462188714386613 7 40 6.792544518399378 7 41 11.521285481319786 7 42 10.616106760073649 7 43 10.758459084115543 7 44 10.254049744247073 7 45 11.061907471834537 7 46 10.663994282976024 7 47 11.89650454621551 7 48 11.078910291787533 7 49 11.96859243659351 7 50 11.094562171828088 7 51 10.88360308548437 7 52 10.748752675390875 7 53 10.27964300083342 7 54 9.56233289753569 7 55 10.621987680772367 7 56 10.158433731251513 8 1 1.5429930241190135 8 2 1.2661450311376896 8 3 0.8291027951079428 8 4 0.7069738418099405 8 5 1.3794144086272269 8 6 2.4793230932426127 8 7 2.0650148294379846 8 8 2.333418599803373 8 9 3.158033077929119 8 10 2.413690126227408 8 11 4.981155239462048 8 12 4.764798821531926 8 13 6.0246703669480475 8 14 5.571973916079543 8 15 6.411701589426116 8 16 7.234214695031863 8 17 7.810125919888353 8 18 8.587642606444799 8 19 7.114156101212199 8 20 6.5682408371358605 8 21 7.315261391571692 8 22 7.683553922667155 8 23 6.496392453676968 8 24 7.550547074849899 8 25 6.8000631159018425 8 26 6.6053200664414256 8 27 5.349332030947288 8 28 5.5370982901172106 8 29 5.85654283902668 8 30 6.076344895693712 8 31 4.336706179877137 8 32 4.83144294263451 8 33 5.6468970807723515 8 34 4.926501139548879 8 35 4.473693385717215 8 36 5.379828933644273 8 37 5.490516014901576 8 38 6.547693716212464 8 39 6.273785012825016 8 40 1.4711159469708086 8 41 9.896213185194853 8 42 9.291930935897824 8 43 8.861119581512881 8 44 10.214720652286399 8 45 9.616274157780168 8 46 11.040512501599505 8 47 12.17976076368226 8 48 11.244323825622121 8 49 12.076892031735213 8 50 10.879408904043238 8 51 11.334730905033243 8 52 11.352136134037494 8 53 11.908763879954297 8 54 11.20649137064153 8 55 11.459465991934888 8 56 9.791314008868635 9 1 1.134423523444255 9 2 1.630854882689647 9 3 1.805148679048564 9 4 1.2710764059125026 9 5 2.043044638046929 9 6 2.704019449517915 9 7 2.2455141546741544 9 8 3.1896939034470932 9 9 3.094267895742881 9 10 2.938656388035774 9 11 4.784124200325749 9 12 5.024920279021799 9 13 5.269272256286779 9 14 5.561852458589669 9 15 6.771013330316805 9 16 7.3964953967862535 9 17 8.246023355785791 9 18 8.15478160779433 9 19 7.19647728879654 9 20 7.122896707581198 9 21 6.679633861207318 9 22 7.263850818753522 9 23 6.829725787010304 9 24 6.848455306968663 9 25 6.70998214424192 9 26 5.991959742554787 9 27 5.259251059287365 9 28 5.407206252330436 9 29 6.301549586665011 9 30 5.467370536719354 9 31 5.394735869485775 9 32 5.6232783002593365 9 33 4.873280346629304 9 34 5.391750802166957 9 35 5.591777056432466 9 36 5.672676437018087 9 37 5.636265002755824 9 38 4.927585753999239 9 39 6.742408494606394 9 40 2.4390646649195276 9 41 8.744728704763006 9 42 8.932956576923466 9 43 9.187030512686967 9 44 10.050753040950372 9 45 8.186449596376661 9 46 11.0357891547709 9 47 11.304929454640423 9 48 10.80235351523076 9 49 11.314746282747357 9 50 10.433390010655927 9 51 9.956863158744444 9 52 10.442891869530072 9 53 10.735686956877377 9 54 11.247314582517376 9 55 10.537738596523287 9 56 9.867224940042725 10 1 1.9421158311365616 10 2 1.6052138570486179 10 3 1.1641230380229217 10 4 1.0403071751432762 10 5 1.8507369457392358 10 6 2.0245322700307398 10 7 2.604488513648512 10 8 2.8820015957547866 10 9 2.966062767537757 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1.4449178237199654 114 21 3.7610818250082225 114 22 4.981799536702237 114 23 4.084627747794613 114 24 2.8944583235599133 114 25 4.669258162341471 114 26 4.164659591725222 114 27 3.6008800185633874 114 28 2.9320931301584885 114 29 3.7314138400586785 114 30 3.6890899937329245 114 31 3.139079760888489 114 32 3.8404728703950823 114 33 3.4366287629188967 114 34 3.9241791581247263 114 35 4.010329092631562 114 36 4.222450192674199 114 37 4.851197129452661 114 38 5.069365542837851 114 39 6.7657870768085075 114 40 7.711312025402183 114 41 8.194200801293928 114 42 6.713499563348805 114 43 5.179489034557257 114 44 -1.5231534447208226 114 45 5.441351557160974 114 46 -1.5652666521672636 114 47 -2.4288563673806927 114 48 0.7623836811432767 114 49 -6.372282374869533 114 50 0.7689857874281749 114 51 2.6371044860445956 114 52 2.675923543738218 114 53 2.3744501544640997 114 54 2.847616241642566 114 55 3.2964112963724546 114 56 5.438114834462031 115 1 1.542744761954168 115 2 1.103036788833819 115 3 1.2345149103015913 115 4 0.7069738418099405 115 5 1.6572197710899843 115 6 2.138707403073795 115 7 2.698828136290018 115 8 2.87909883813505 115 9 2.746662670779166 115 10 2.6798271669424025 115 11 2.7214730150330553 115 12 -1.1565020722118717 115 13 0.5924459379510303 115 14 -5.096347831686096 115 15 -5.792121647912513 115 16 3.3556148936415973 115 17 4.810125919888353 115 18 5.621157998699022 115 19 4.286946567945069 115 20 4.662083935447676 115 21 7.091825443210222 115 22 7.315132870035573 115 23 6.005826415941122 115 24 7.168968127481481 115 25 6.352942957014058 115 26 5.7691730952398395 115 27 5.6852307399840285 115 28 5.40527108058394 115 29 5.560862600695003 115 30 5.901816593806917 115 31 5.099864074613979 115 32 5.665852078682171 115 33 5.53244822277831 115 34 5.533502132597537 115 35 5.522352770027052 115 36 5.112202319940199 115 37 5.0520850317834025 115 38 6.306879416311773 115 39 6.870855519279836 115 40 8.806021607348168 115 41 9.375110901182936 115 42 8.508912067973299 115 43 7.345230802962732 115 44 0.33885173470943997 115 45 8.475757772477287 115 46 5.224226503585605 115 47 -6.221679156873851 115 48 4.375406248660852 115 49 1.3720757457371988 115 50 5.852596590239866 115 51 4.9375114412795185 115 52 6.910477742776322 115 53 7.00782048372788 115 54 5.826898520591879 115 55 5.719644740693578 115 56 6.6763686265449 116 1 1.81892742533946 116 2 1.8449017054321217 116 3 1.329674877487804 116 4 0.866394131665011 116 5 1.7392542255608632 116 6 2.4816114227620645 116 7 2.7639088035035826 116 8 2.960039499879649 116 9 3.497277929187703 116 10 2.351142452695754 116 11 4.043321524740463 116 12 3.235622620158928 116 13 4.325013616375966 116 14 4.054048668177181 116 15 -0.7852854433732723 116 16 5.302292498235527 116 17 6.056502731482556 116 18 5.829252064873483 116 19 5.64352299671182 116 20 5.453442972910075 116 21 5.569265968230727 116 22 6.097741565687745 116 23 5.6268272362856635 116 24 6.139982620235106 116 25 5.540528409570797 116 26 4.879919608775527 116 27 5.1550147159205935 116 28 4.522033454114155 116 29 4.334994402718522 116 30 5.377069533374868 116 31 4.752037987657456 116 32 4.357949426234811 116 33 4.548308217309351 116 34 5.556745228030952 116 35 5.1441739349163065 116 36 6.242910550730468 116 37 6.095016396289825 116 38 7.0446426101865285 116 39 7.695028338530584 116 40 10.148652178854864 116 41 10.28541989762811 116 42 7.342655573578984 116 43 4.904979230635686 116 44 4.160563520326065 116 45 7.882101770289701 116 46 2.6896353086170492 116 47 -3.2285153614130913 116 48 1.5219744739821515 116 49 -1.0815747874867547 116 50 2.796266264836529 116 51 6.264555466436754 116 52 6.311899673320486 116 53 6.1587638799542965 116 54 5.153669097567544 116 55 6.309199020157621 116 56 8.244593947846514 117 1 0.9549363439570726 117 2 -0.010170758335995345 117 3 -0.008953885053998079 117 4 -0.1584107735746727 117 5 0.6840702790725643 117 6 1.0117117572102217 117 7 1.4121808213408187 117 8 1.7089246726778669 117 9 1.9404217418967278 117 10 2.252758952138336 117 11 0.18156009776164694 117 12 -6.975079720978197 117 13 -8.532009794995272 117 14 -1.7266090798718707 117 15 -8.594371285067812 117 16 -7.629145628854776 117 17 0.9062797660422 117 18 -2.967013264000542 117 19 0.5362208785401315 117 20 0.06520439988889493 117 21 4.141172322745778 117 22 4.4497482546509595 117 23 5.419469376753888 117 24 5.457429665943021 117 25 4.49844368270346 117 26 3.5688828194778672 117 27 5.528481828518139 117 28 4.144385739509918 117 29 4.115652150767573 117 30 5.018652588001405 117 31 2.3690948438447457 117 32 2.7130218900029277 117 33 1.7258444491934064 117 34 2.455853366269526 117 35 2.675110389765802 117 36 3.877804642146298 117 37 4.347803464294284 117 38 4.59425242066591 117 39 5.434716186914088 117 40 6.663423639278502 117 41 7.73831844835275 117 42 5.734238628205517 117 43 -3.8898925642361064 117 44 0.8584453486426789 117 45 6.545423955351019 117 46 0.2024558214375638 117 47 0.9651858648968314 117 48 0.5267124895897339 117 49 0.9301308981319742 117 50 2.600056677322595 117 51 5.944042645923933 117 52 6.853148279786481 117 53 5.54337926456968 117 54 6.894750479953274 117 55 5.473636032420718 117 56 7.597994170811955 118 1 0.5178734068941324 118 2 0.6261928780276378 118 3 0.3494377233376085 118 4 0.116064750900847 118 5 0.7382660832683712 118 6 1.674299169797635 118 7 2.149943059103059 118 8 2.569064532817727 118 9 2.148463699938688 118 10 2.569192518571903 118 11 1.7969447131462601 118 12 -3.3859188818173607 118 13 -4.2085832215686985 118 14 -10.072762926025717 118 15 -5.916049606746132 118 16 -7.255019754728899 118 17 0.17376228352471657 118 18 -6.793936340923619 118 19 -0.5389539466346918 118 20 -0.0047256700411750785 118 21 4.230333161906618 118 22 6.019678324581026 118 23 4.882756090040601 118 24 5.600786309299661 118 25 4.900541584801367 118 26 5.956994707589754 118 27 4.1543559543922655 118 28 3.971308816432998 118 29 5.007260542375967 118 30 4.644526713875532 118 31 4.713500438250346 118 32 5.031203708184748 118 33 4.8167535401025 118 34 4.866692527108686 118 35 4.078956543611955 118 36 5.369063383405038 118 37 4.947453813944637 118 38 4.7271195535330435 118 39 6.141009893207794 118 40 6.789297765152629 118 41 6.715591175625477 118 42 6.223749117716007 118 43 0.9580095336659902 118 44 -0.5663798261824979 118 45 5.125843535770599 118 46 -0.21187984289809947 118 47 0.9249760746870415 118 48 1.6892999021771473 118 49 -0.2604285424274657 118 50 2.143762971028888 118 51 5.461525163406449 118 52 6.232518909157111 118 53 5.817854789045207 118 54 5.934960270163064 118 55 6.648461207245894 118 56 10.251840324658108 119 1 1.0695668115287162 119 2 0.6761037514679273 119 3 -0.02667635865882545 119 4 0.29520913592758546 119 5 0.6531502187407412 119 6 1.1738535369990615 119 7 2.2123316509034154 119 8 2.1595279909282397 119 9 2.3378576393326256 119 10 2.7305115916556844 119 11 2.247925105303125 119 12 -8.904189826558891 119 13 -2.1515422233512282 119 14 -3.9502139064178756 119 15 -7.66337580995469 119 16 -9.201543818900024 119 17 -0.4643838840332144 119 18 -6.59785790955107 119 19 -0.26355287711597697 119 20 -0.26096452922121216 119 21 4.015983785792539 119 22 4.471995615133611 119 23 4.26109833602991 119 24 3.3650465588540293 119 25 4.061415025086568 119 26 5.399953709372287 119 27 5.208723155818291 119 28 4.794838228197701 119 29 5.809845212607698 119 30 4.6890899937329245 119 31 4.1194719177512376 119 32 3.5463552233362634 119 33 4.691530723703213 119 34 4.198688962046294 119 35 4.324054582827639 119 36 5.457744310321257 119 37 5.125706933374229 119 38 5.206620444798638 119 39 6.1187282532790945 119 40 7.417194378343357 119 41 6.605965507176279 119 42 5.713499563348805 119 43 -3.153844298776079 119 44 -0.18982011138749044 119 45 5.264880968925677 119 46 0.16022354391116878 119 47 -2.781797543851283 119 48 -5.433694750229272 119 49 -3.5487529631048282 119 50 -0.13297499688555092 119 51 5.048869191926947 119 52 5.577884328051944 119 53 5.178371723091551 119 54 4.98487114360335 119 55 5.472881884607748 119 56 8.889095226618892 120 1 1.1872138703522452 120 2 0.6368880651934177 120 3 0.4439118766352905 120 4 0.1579542339668052 120 5 1.0845227677603475 120 6 1.5660103997441581 120 7 2.486841454824983 120 8 2.237959363477259 120 9 2.3182497961953743 120 10 1.7305115916556844 120 11 -0.08540822803021086 120 12 -5.355170218715756 120 13 -6.465267713547309 120 14 -5.8521746907316015 120 15 0.43466340573158746 120 16 -6.946641858115711 120 17 0.49640042969227594 120 18 -4.891975556609893 120 19 -0.38119993593950596 120 20 0.24883939234741703 120 21 3.8787288838317515 120 22 5.393564242584592 120 23 4.790510100735794 120 24 4.365046558854029 120 25 4.335924829008135 120 26 4.321522336823268 120 27 4.365585900916329 120 28 4.206602934080056 120 29 5.025531487117497 120 30 5.1008546996152795 120 31 3.9430013295159405 120 32 3.1541983605911597 120 33 4.868001311938503 120 34 4.551630138516881 120 35 4.20640752400411 120 36 4.908724702478121 120 37 4.615903011805596 120 38 5.461522405582954 120 39 6.903041978769288 120 40 8.06425320187277 120 41 -5.51168155164725 120 42 -4.678657299396292 120 43 -9.232275671325098 120 44 -15.346682856485527 120 45 -4.107668050682165 120 46 -9.094678416873146 120 47 -6.840621073263048 120 48 -5.2180084757194685 120 49 -14.627184335653848 120 50 -3.8388573498267284 120 51 -0.5393661021906979 120 52 -0.46133135822256577 120 53 -0.19417729651629045 120 54 -0.15238375835743412 120 55 -0.27221615460793913 120 56 3.3400756187757565 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 251.0 5 71.0 6 345.0 7 204.0 8 330.0 9 527.0 10 314.0 11 191.0 12 268.0 13 129.0 14 197.0 15 201.0 16 232.0 17 320.0 18 352.0 19 462.0 20 495.0 21 734.0 22 960.0 23 1136.0 24 1413.0 25 1636.0 26 1798.0 27 1868.0 28 2066.0 29 2102.0 30 2349.0 31 2702.0 32 2956.0 33 3323.0 34 3640.0 35 3919.0 36 4226.0 37 4861.0 38 4731.0 39 4051.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 0.0 0.0 0.0 100.0 3 100.0 0.0 0.0 0.0 4 0.0 0.0 100.0 0.0 5 0.0 0.0 0.0 100.0 6 0.0 0.0 0.0 100.0 7 99.42559878617102 0.35583974567392795 0.11560276001589538 0.10295870813915682 8 99.33888477448023 0.48409530174671705 0.1065732194143892 0.07044670435866404 9 97.48740087787432 0.9808348837629379 0.9663842777406478 0.5653799606220986 10 44.66962898739207 22.318557855568802 24.42288934648315 8.588923810555977 11 28.620714569560345 34.31776308659369 16.155485712221378 20.90603663162458 12 25.50485892850692 20.635092662837327 18.037643148730176 35.82240525992558 13 20.346085762797586 15.541346049636934 20.644124128463567 43.46844405910191 14 20.373180159676313 25.36577435786279 32.46270004696362 21.798345435497275 15 38.82577132486389 18.192377495462793 19.773139745916517 23.208711433756807 16 35.94145283700759 24.981929887965304 19.615106613661005 19.4615106613661 17 23.34501698345017 19.561682445616825 38.61024788610248 18.48305268483053 18 21.718868537986342 19.598280408944763 32.29652107944077 26.38632997362812 19 34.239297395955695 21.396172542783308 18.774057140791875 25.590472920469125 20 21.28728982100886 34.83818477671307 24.064364491050444 19.810160911227626 21 20.393049384054045 21.782088797370037 32.843827896391026 24.981033922184892 22 20.508652144069938 20.90603663162458 25.000903146562624 33.58440807774286 23 19.820454463350313 23.04649398504389 24.054405548932483 33.07864600267331 24 19.888365035495585 22.85987824924583 30.961541935367332 26.290214779891258 25 32.89799309958634 21.570115067107427 23.947325638107625 21.58456619519861 26 27.859608537709423 22.289494135294863 17.463989445338058 32.38690788165766 27 21.316365082521376 27.189573202697083 16.950776405936477 34.543285308845064 28 23.575737870741666 38.42707994653372 18.02138651060294 19.97579567212167 29 38.92828714089298 25.329256820319852 16.978435487372458 18.76402055141472 30 25.25259828287393 22.926344328965207 33.00316312697695 18.817894261183913 31 24.889804176602357 38.53602138882867 18.74954837777296 17.82462605679601 32 39.26790489267905 25.53841150538412 17.774806677748067 17.41887692418877 33 24.547920433996385 26.122965641952984 18.216998191681736 31.112115732368895 34 20.692462147935096 36.20592970460013 25.945623270201335 17.155984877263435 35 21.024351412960883 24.394833013713498 38.276585736512644 16.304229836812965 36 23.301830535427097 36.509514085906865 20.918339688104233 19.27031569056181 37 23.782219408519488 23.782219408519488 35.09993669168852 17.335624491272497 38 36.368408667161454 25.29370236953098 20.411635863367305 17.926253099940265 39 24.763506791831716 23.215222385009135 18.242986597210916 33.778284225948234 40 19.091587899979967 19.634304030304687 30.415778834070917 30.858329235644426 41 18.058546556192283 16.82118480125561 27.727488411139905 37.392780231412196 42 30.69733743886977 25.667451548632492 21.02517659844231 22.610034414055423 43 16.813966966096782 22.89553752535497 34.43748188930745 25.8530136192408 44 18.125419485914342 24.76463438968201 23.302555916338637 33.807390208065016 45 21.861029158898802 23.12621042914804 34.952668826585096 20.060091585368063 46 18.461956521739133 26.570652173913047 21.952898550724637 33.01449275362319 47 17.008259961877098 33.065262775710266 20.557320504674596 29.36915675773804 48 22.091489092519293 34.70151276907228 21.63603173742522 21.57096640098321 49 17.71182297231226 35.213588541006466 20.799304637560436 26.27528384912084 50 29.39398874472975 26.279789370826773 22.821779490798544 21.504442393644936 51 19.111199681147866 37.23142142831262 22.7526359650712 20.904742925468316 52 19.81184984170059 28.43238353686115 32.82134780642244 18.93441881501583 53 18.725438707691193 27.112044767199695 34.1802640395516 19.98225248555751 54 19.81282357626444 35.81695087071431 23.99985518265088 20.37037037037037 55 18.31682271764131 34.59260734918594 24.856475360849014 22.23409457232374 56 30.546000941380935 23.469350809225535 25.134870922191244 20.849777327202286 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.5 9 1.0 10 3.0 11 5.0 12 11.0 13 89.0 14 161.0 15 247.5 16 334.0 17 416.0 18 498.0 19 498.0 20 498.0 21 449.5 22 356.0 23 311.0 24 303.0 25 295.0 26 267.5 27 240.0 28 229.0 29 234.5 30 251.0 31 280.0 32 309.0 33 329.0 34 349.0 35 344.5 36 340.0 37 384.5 38 561.0 39 693.0 40 717.5 41 742.0 42 785.5 43 829.0 44 911.0 45 993.0 46 1169.5 47 1733.5 48 2121.0 49 4154.5 50 6188.0 51 5659.5 52 5131.0 53 4133.0 54 2836.0 55 2537.0 56 2714.5 57 2892.0 58 2941.0 59 2990.0 60 2921.0 61 2852.0 62 3012.0 63 3184.5 64 3197.0 65 3110.5 66 3024.0 67 2716.0 68 2408.0 69 2200.0 70 1992.0 71 1662.5 72 1215.5 73 1098.0 74 937.5 75 777.0 76 683.0 77 589.0 78 456.5 79 248.0 80 172.0 81 127.5 82 83.0 83 72.0 84 61.0 85 41.0 86 21.0 87 12.5 88 2.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.5 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0018062931252483653 9 0.0018062931252483653 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.4732487988150717 16 0.039738448755464034 17 0.025288103753477113 18 0.0 19 0.04515732813120913 20 0.093927242512915 21 0.0 22 0.0 23 0.0 24 0.005418879375745096 25 0.005418879375745096 26 0.05599508688269932 27 0.0776706043856797 28 0.0 29 0.1553412087713594 30 0.06683284563418952 31 0.010837758751490192 32 0.025288103753477113 33 0.11199017376539865 34 0.14630974314511758 35 0.15895379502185614 36 0.0415447418807124 37 0.13908457064412413 38 0.21494888190455547 39 0.1354719843936274 40 0.8182507857375095 41 1.0259744951410714 42 0.27455655503775156 43 0.2637187962862613 44 0.4244788844333659 45 0.20411112315306526 46 0.29261948629023515 47 0.5003431956937971 48 0.05960767313319606 49 0.25107474440952277 50 0.17882301939958817 51 0.2962320725407319 52 0.15714750189660778 53 0.25829991691051624 54 0.21675517502980382 55 0.261912503161013 56 0.2239803475307973 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 55362.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.61926953506014 #Duplication Level Percentage of deduplicated Percentage of total 1 89.19687277896233 61.20624254904086 2 6.554528942588645 8.99533976373686 3 1.7478743846903053 3.598135905494744 4 0.7607465318908104 2.08807485278711 5 0.42643923240938164 1.4630974314511758 6 0.2474400484350733 1.0187493226400781 7 0.1605727973887178 0.771287164481052 8 0.1763668430335097 0.9681731151331239 9 0.10266129669114744 0.6340088869621763 >10 0.5554239385085157 7.026480257216142 >50 0.04211745505277843 2.032079765904411 >100 0.02369106846718787 3.861854701781005 >500 0.002632340940798652 1.2878869983020844 >1k 0.002632340940798652 5.048589285069181 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCGTCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 2795 5.048589285069181 No Hit GCGTCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 713 1.2878869983020844 No Hit GCGTCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG 386 0.697229146345869 No Hit GCGTCCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT 364 0.6574906975904049 No Hit GCGTCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG 350 0.6322025938369278 No Hit GCGTCCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC 348 0.6285900075864311 No Hit GCGTCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG 226 0.4082222463061305 No Hit GCGTCCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC 127 0.22939922690654238 No Hit GCGTCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 114 0.20591741627831364 No Hit GCGTCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 112 0.2023048300278169 No Hit GCGTCCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC 111 0.20049853690256852 No Hit GCGTCCGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATG 97 0.17521043314909143 No Hit GCGTCCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA 96 0.17340414002384308 No Hit GCGTCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCGTCTTCTGCTTGGATC 96 0.17340414002384308 No Hit GCGTCCGGGGCCTGGAGGCCCATCTGGTACAGCTGGCAGATGCCTGGGACTGTAAG 90 0.16256638127235287 No Hit GCGTCCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT 80 0.14450345001986922 No Hit GCGTCCGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT 73 0.13185939814313066 No Hit GCGTCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA 66 0.11921534626639212 No Hit GCGTCCGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC 66 0.11921534626639212 No Hit GCGTCCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 61 0.11018388064015028 No Hit GCGTCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG 61 0.11018388064015028 No Hit GCGTCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 59 0.10657129438965354 No Hit GCGTCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTACG 57 0.10295870813915682 No Hit GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 57 0.10295870813915682 No Hit GCGTCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCCGTCTCAAAGATTAAG 56 0.10115241501390845 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0036125862504967307 0.0 0.0 0.0 0.0 11 0.010837758751490192 0.0 0.0 0.0 0.0 12 0.012644051876738557 0.0 0.0 0.0 0.0 13 0.014450345001986923 0.0 0.0 0.0 0.0 14 0.03070698312922221 0.0 0.0 0.0 0.0 15 0.03431956937971894 0.0 0.0 0.0 0.0 16 0.036125862504967306 0.0 0.0 0.0 0.0 17 0.036125862504967306 0.0 0.0 0.0 0.0 18 0.036125862504967306 0.0 0.0 0.0 0.0 19 0.0415447418807124 0.0 0.0 0.0 0.0 20 0.04335103500596077 0.0 0.0 0.0 0.0 21 0.0469636212564575 0.0 0.0 0.0 0.0 22 0.0469636212564575 0.0 0.0 0.0 0.0 23 0.05418879375745096 0.0 0.0 0.0 0.0 24 0.05599508688269932 0.0 0.0 0.0 0.0 25 0.05960767313319606 0.0 0.0 0.0 0.0 26 0.06322025938369279 0.0 0.0 0.0 0.0 27 0.06683284563418952 0.0 0.0 0.0 0.0 28 0.07405801813518298 0.0 0.0 0.0 0.0 29 0.07947689751092807 0.0 0.0 0.0 0.0 30 0.08128319063617644 0.0 0.0 0.0 0.0 31 0.08670207001192154 0.0 0.0 0.0 0.0 32 0.0885083631371699 0.0 0.0 0.0 0.0 33 0.0885083631371699 0.0 0.0 0.0 0.0 34 0.093927242512915 0.0 0.0 0.0 0.0 35 0.09573353563816336 0.0 0.0 0.0 0.0 36 0.09753982876341173 0.0 0.0 0.0 0.0 37 0.09934612188866009 0.0 0.0 0.0 0.0 38 0.09934612188866009 0.0 0.0 0.0 0.0 39 0.10115241501390845 0.0 0.0 0.0 0.0 40 0.10295870813915682 0.0 0.0 0.0 0.0 41 0.10657129438965356 0.0 0.0 0.0 0.0 42 0.10837758751490192 0.0 0.0 0.0 0.0 43 0.11018388064015028 0.0 0.0 0.0 0.0 44 0.11921534626639212 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCAATCC 20 3.9040158E-4 50.619484 41 ACGCCTG 15 0.008018794 50.573 40 GCGCATT 15 0.008018794 50.573 38 CATGGGT 15 0.008018794 50.573 40 CGCATTG 15 0.008018794 50.573 39 TGCCCGT 25 1.9372983E-5 50.526608 37 GGCGCAT 15 0.008047978 50.526604 37 TGCTCGT 75 0.0 50.526604 37 ATGCCCG 20 3.9575202E-4 50.48029 36 TATGCTC 85 0.0 50.387924 35 TATGCTG 15 0.008136008 50.38792 35 TATGCCC 30 9.760606E-7 50.38792 35 ATTGCTT 20 4.0116056E-4 50.341866 34 ATATGCC 100 0.0 50.341866 34 CAAGATT 45 1.2551027E-10 50.204193 47 CAAAGAC 15 0.008254493 50.204193 47 TCCACGA 25 2.0231373E-5 50.15847 45 ACGATTA 35 5.03951E-8 50.15847 48 AGCAAGC 15 0.008284314 50.15847 48 GCCCAAA 20 4.0846315E-4 50.158466 48 >>END_MODULE