FastQCFastQC Report
Fri 17 Jun 2016
SRR1525925_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525925_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2994
Sequences flagged as poor quality0
Sequence length40
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTCCGGGGTGGGAGTCGGCTCTCTTCTTCTGTGTGTTC80.26720106880427524No Hit
GCGTCCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT70.23380093520374082No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCCGAGTCGACTTTT60.2004008016032064No Hit
GCGTCCGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.2004008016032064No Hit
GCGTCCGGGTCGACTTTTCTTTTTTTTTTTTTTTTTTTTT50.16700066800267202No Hit
GCGTCCGGGCTCGTCTTAATCAGCAACATCTTTTGATACA40.13360053440213762No Hit
GCGTCCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT40.13360053440213762No Hit
GCGTCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG40.13360053440213762No Hit
GCGTCCGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT40.13360053440213762No Hit
GCGTCCGGGGGGGTTGGGGATTTAGCTCCGTGGTAGAGCG30.1002004008016032No Hit
GCGTCCGGGGGGGTTGGGGATTTAGCTCCGTGGTAGAGCC30.1002004008016032No Hit
GCGTCCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT30.1002004008016032No Hit
GCGTCCGGGGATGACCCTTGGCTTTATGTGTGGAGTTGGG30.1002004008016032No Hit
GCGTCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCC30.1002004008016032No Hit
GCGTCCGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT30.1002004008016032No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGGG600.033.5762755
CCGGGGA301.138591E-533.5762755
CGGGGGG404.902904E-833.576276
CGGGGAG251.7017999E-433.576276
TCCGGGT357.522758E-733.576274
TCCGGGG1050.033.576274
TCCGGGC551.2732926E-1133.576274
CGGGAAG200.002514073933.576276
CCGGGCT251.7017999E-433.576275
CCGGGAG200.002514073933.576275
GCGTCCG3100.031.951611
CGTCCGG3050.031.9249782
GTCCGGG2850.031.80913
TCCGGGA900.027.9802274
CCGGGAC250.007500197326.8610155
CCGGGAA350.001223949423.9830515
GGGGGGG500.00971201816.7881367
Produced by FastQC (version 0.11.5)