FastQCFastQC Report
Fri 17 Jun 2016
SRR1525924_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525924_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1133
Sequences flagged as poor quality0
Sequence length40
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTCCGGGGGGCTGGTGAGATGGCCCAGTGGGTAAGAGC30.264783759929391No Hit
GCGTCCGGGTGGACGTCGTTTTTTTTTTTTTTTTTTTTTT30.264783759929391No Hit
GCGTCCGGGGCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.264783759929391No Hit
GCGTCCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT20.176522506619594No Hit
GCGTCCGGGTGTCTGCCTGAAAAAAAAAAAAAAAAAAAAA20.176522506619594No Hit
GCGTCCGGGACGCCGAGTCGACTTTTTTTTTTTTTTTTTT20.176522506619594No Hit
GCGTCCGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT20.176522506619594No Hit
GCGTCCGGGAGTCTTCTTTTTTTTTTTTTTTTTTTTTTTT20.176522506619594No Hit
GCGTCCGGGGGCCTTTTTTTTTTTTTTTTTTTTTTTTTTT20.176522506619594No Hit
GCGTCCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT20.176522506619594No Hit
GCGTCCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT20.176522506619594No Hit
GCGTCCGGGGAGTCGATTTTTTTTTTTTTTTTTTTTTTTT20.176522506619594No Hit
GCGTCCGGGGTCCCCTTTTTTTTTTTTTTTTTTTTTTTTT20.176522506619594No Hit
GCGTCCGGGGCGTCGCCTTTTTTTTTTTTTTTTTTTTTTT20.176522506619594No Hit
GCGTCCGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.176522506619594No Hit
GCGTCCGGGCGTCGCCTTTTTTTTTTTTTTTTTTTTTTTT20.176522506619594No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGGGC200.001807715333.7727284
TCCGGGA401.396802E-833.7727284
GTCCGGG1000.033.7727243
TCCGGGG305.626476E-633.7727244
GCGTCCG1050.033.7727241
CCGGGAG305.626476E-633.7727245
CGTCCGG1050.033.7727242
Produced by FastQC (version 0.11.5)