FastQCFastQC Report
Fri 17 Jun 2016
SRR1525923_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525923_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4218
Sequences flagged as poor quality0
Sequence length40
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT2425.737316263632053No Hit
GCGTCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA451.0668563300142246No Hit
GCGTCCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG380.9009009009009009No Hit
GCGTCCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT350.8297771455666193No Hit
GCGTCCGGGGCCTGGAGGCCCATCTGGTACAGCTGGCAGA260.6164058795637744No Hit
GCGTCCGGGTACCTGGTTGATACTGCCAGTAGCATATGCT220.5215742057847321No Hit
GCGTCCGGGTCCCTGGTTGATCCTGCCAGTAGCATATGCT220.5215742057847321No Hit
GCGTCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA210.4978662873399715No Hit
GCGTCCGGGTAACTGGTTGATCCTGCCAGTAGCATATGCT190.45045045045045046No Hit
GCGTCCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA170.40303461356092934No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTT100.2370791844476055No Hit
GCGTCCGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT90.21337126600284498No Hit
GCGTCCGGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.21337126600284498No Hit
GCGTCCGGGTAACTGGTTGATACTGCCAGTAGCATATGCT80.1896633475580844No Hit
GCGTCCGGGTACATGGTTGATCCTGCCAGTAGCATATGCT80.1896633475580844No Hit
GCGTCCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.16595542911332387No Hit
GCGTCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC70.16595542911332387No Hit
GCGTCCGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG70.16595542911332387No Hit
GCGTCCGGGCGTTTCTTTGGTTTTTACAGCAGCCTTAATT60.1422475106685633No Hit
GCGTCCGGGTCACAGATCCTCTGAAGGAGATTGGGCGGAT60.1422475106685633No Hit
GCGTCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAACG60.1422475106685633No Hit
GCGTCCGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.1422475106685633No Hit
GCGTCCGGGTACCTGGTTCATCCTGCCAGTAGCATATGCT60.1422475106685633No Hit
GCGTCCGGGGTGTTCTTTATTAATATCCTAACACTCCTCG50.11853959222380275No Hit
GCGTCCGGGGCCTGGAGGCCCCTCTGGTACAGCTGGCAGA50.11853959222380275No Hit
GCGTCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG50.11853959222380275No Hit
GCGTCCGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGG50.11853959222380275No Hit
GCGTCCGGGATGCCACAACTAGATACATCAACATGATTTA50.11853959222380275No Hit
GCGTCCGGGCAGGTCCTTGGGGTTCACTCTAGACTCAAGC50.11853959222380275No Hit
GCGTCCGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.11853959222380275No Hit
GCGTCCGGGTAAATGGTTGATCCTGCCAGTAGCATATGCT50.11853959222380275No Hit
GCGTCCGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.11853959222380275No Hit
GCGTCCGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT50.11853959222380275No Hit
GCGTCCGGGTACCTGGTTGATCCTGCCATTAGCATATGCT50.11853959222380275No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGGA200.002485876434.06
CGGGGAG301.1634544E-534.06
CGGGGAA251.7057777E-434.06
TCCGGGC601.8189894E-1234.04
TCCGGGA700.034.04
CGGGATT301.1634544E-534.06
CCGGGTA251.7057777E-434.05
CCGGGGG551.4551915E-1134.05
CCGGGCA200.002485876434.05
CCGGGAT453.5342964E-934.05
GCGTCCG4250.033.21
CGTCCGG4200.033.1904752
GTCCGGG4050.032.7407383
CCGGGGA750.031.7333345
TCCGGGG2150.031.6279074
TCCGGGT653.6379788E-1231.3846154
CCGGGGC451.4940997E-730.2222215
CCGGGTC402.2403983E-629.755
CCGGGGT402.2403983E-629.755
CGGGTCA304.975698E-428.3333326
Produced by FastQC (version 0.11.5)