FastQCFastQC Report
Fri 17 Jun 2016
SRR1525922_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525922_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53946
Sequences flagged as poor quality0
Sequence length56
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC4100.760019278537797No Hit
GCCTAGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT3800.7044081118155192No Hit
GCCTAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG2890.5357209060912764No Hit
GCCTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2830.5245986727468209No Hit
GCCTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA1550.2873243613984355No Hit
GCCTAGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC1500.2780558336113892No Hit
GCCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC1060.1964927890853817No Hit
GCCTAGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA1010.18722426129833536No Hit
GCCTAGGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT930.1723946168390613No Hit
GCCTAGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT930.1723946168390613No Hit
GCCTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC920.17054091128165202No Hit
GCCTAGGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT740.1371742112482853No Hit
GCCTAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG700.12975938901864828No Hit
GCCTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAAA690.12790568346123904No Hit
GCCTAGGGGACTAATCCTAGCCCTAGCCCTACACAAATATCATTATACTATTATAT570.10566121677232788No Hit
GCCTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA570.10566121677232788No Hit
GCCTAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC560.10380751121491863No Hit
GCCTAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT540.10010010010010009No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAATC309.692649E-750.4350340
TATAATT150.00810475150.4350338
TGTCCAA309.692649E-750.4350338
GCGCATT354.821777E-850.43502438
CAGGTAT150.00816533350.3402344
GTCAGGT150.00816533350.3402342
ACCAACA204.0112468E-450.34022542
ATCCTAG150.00825683750.19869214
TTACCGA150.00825683750.19869249
TATTATA204.067466E-450.1986949
AGAGCGC150.00831826250.10477435
TGGCTAC204.1052917E-450.1047735
TAGAGCG150.00834910250.05794534
ACTAATC204.1243093E-450.05794510
GACTCAC150.00834910250.05794510
ATGGGCC150.00838002750.01120850
GGACAAT150.00838002750.0112089
TACCGAA150.00838002750.01120850
GGCTTAC150.00847331249.8715127
AACCACC150.00850457749.82511517
Produced by FastQC (version 0.11.5)