##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1525922_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 53946 Sequences flagged as poor quality 0 Sequence length 56 %GC 53 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.023579134690245 38.0 35.0 39.0 29.0 40.0 2 35.81551922292663 38.0 35.0 39.0 28.0 40.0 3 36.174081488896306 39.0 36.0 39.0 29.0 40.0 4 35.979868757646535 39.0 36.0 39.0 29.0 40.0 5 33.20003336670003 38.0 31.0 39.0 15.0 40.0 6 35.80734438141845 38.0 35.0 39.0 28.0 40.0 7 35.81225670114559 38.0 35.0 39.0 29.0 40.0 8 35.63797130463797 38.0 35.0 39.0 28.0 40.0 9 35.666129092055016 38.0 35.0 39.0 28.0 40.0 10 35.18084751418085 38.0 35.0 39.0 25.0 40.0 11 34.6674266859452 38.0 34.0 39.0 25.0 40.0 12 33.75032439847255 38.0 32.0 39.0 21.0 40.0 13 33.37098209320432 38.0 32.0 39.0 19.0 40.0 14 33.9923441960479 38.0 33.0 39.0 22.0 40.0 15 32.941867793719645 38.0 31.0 39.0 17.0 40.0 16 32.38662736810885 37.0 30.0 39.0 17.0 40.0 17 31.342954065176286 36.0 27.0 39.0 14.0 40.0 18 30.811885960034108 35.0 26.0 38.0 13.0 40.0 19 31.910558706855003 36.0 29.0 38.0 17.0 40.0 20 32.61908204500797 36.0 30.0 39.0 19.0 40.0 21 32.73614355095837 38.0 30.0 39.0 18.0 40.0 22 31.98748748748749 37.0 29.0 39.0 15.0 40.0 23 32.66314462610759 37.0 30.0 39.0 17.0 40.0 24 32.00661772883995 37.0 29.0 39.0 16.0 40.0 25 32.48098098098098 37.0 30.0 39.0 17.0 40.0 26 32.800411522633745 38.0 31.0 39.0 17.0 40.0 27 33.07818930041152 38.0 32.0 39.0 19.0 40.0 28 33.10132354576799 38.0 32.0 39.0 19.0 40.0 29 32.54621287954621 37.0 31.0 39.0 17.0 40.0 30 32.35819152485819 37.0 31.0 39.0 17.0 40.0 31 33.02224446668891 38.0 33.0 39.0 17.0 40.0 32 32.949264078893705 38.0 32.0 39.0 17.0 40.0 33 32.720646572498424 37.0 32.0 39.0 17.0 40.0 34 32.55388722055389 37.0 32.0 39.0 16.0 40.0 35 32.43449004560116 37.0 31.0 39.0 15.0 40.0 36 31.915508100693287 36.0 31.0 38.0 9.0 40.0 37 31.61101842583324 36.0 30.0 38.0 4.0 40.0 38 31.03668483298113 36.0 29.0 38.0 4.0 40.0 39 29.89343046750454 35.0 27.0 38.0 4.0 40.0 40 27.968802135468803 34.0 19.0 38.0 4.0 39.0 41 26.18661253846439 31.0 15.0 38.0 4.0 39.0 42 26.97034071108145 32.0 19.0 38.0 4.0 39.0 43 27.068179290401513 33.0 19.0 38.0 4.0 39.0 44 26.125199273347423 31.0 15.0 37.0 4.0 39.0 45 26.982352723093463 33.0 19.0 38.0 4.0 39.0 46 25.162217773328884 30.0 13.0 36.0 4.0 39.0 47 24.137897156415676 29.0 10.0 36.0 4.0 39.0 48 24.75722018314611 30.0 11.0 36.0 4.0 39.0 49 23.821247173099025 29.0 6.0 36.0 4.0 39.0 50 24.411263114966818 29.0 4.0 36.0 4.0 39.0 51 24.644162681199717 30.0 4.0 36.0 4.0 39.0 52 24.477681385088793 30.0 4.0 36.0 4.0 39.0 53 24.28552626700775 30.0 4.0 36.0 4.0 39.0 54 23.901290179067956 30.0 4.0 36.0 4.0 39.0 55 23.673265858451042 30.0 4.0 36.0 4.0 39.0 56 22.84693953212472 29.0 4.0 36.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 3.9674246792441963 1 2 4.051583687659651 1 3 3.5753954441853892 1 4 3.6994393993284262 1 5 7.391005383895713 1 6 3.864218321524227 1 7 4.190663559913233 1 8 4.466071385452722 1 9 4.309735801457343 1 10 4.665458452228151 1 11 -0.5358067714243049 1 12 -6.357530521645398 1 13 -17.63098287408192 1 14 -6.778001177772445 1 15 0.6641417735069766 1 16 -1.0722776276116903 1 17 -6.014050078875709 1 18 -4.045661614175074 1 19 0.5692908648977131 1 20 -0.15918936320617405 1 21 5.983847129819981 1 22 6.8599274723531956 1 23 6.211149735930757 1 24 6.592036174799173 1 25 6.311467873231038 1 26 6.028199399133669 1 27 5.164189720208064 1 28 5.166457066763417 1 29 5.307209325030929 1 30 6.226805614547587 1 31 4.262095909572363 1 32 3.680430072105807 1 33 4.387230841357152 1 34 4.309548013888371 1 35 4.677505691171785 1 36 3.632220972284241 1 37 3.5606497736754825 1 38 4.655213408846702 1 39 4.291774046633201 1 40 -9.510897666487175 1 41 -1.3798835079840792 1 42 -1.898253061124695 1 43 -9.729464771231815 1 44 -14.987140573511518 1 45 -2.769488424092426 1 46 -5.2463601335698336 1 47 -10.720756406364977 1 48 -5.713893314072312 1 49 -13.333992217311348 1 50 -3.965608175875804 1 51 2.306094201710099 1 52 1.880175647471308 1 53 1.7122463608459952 1 54 2.5279952659830265 1 55 1.391799839347712 1 56 4.501386580186928 2 1 4.743895267479488 2 2 4.286877805306709 2 3 4.0812777971265675 2 4 4.181792340504892 2 5 7.75571126624865 2 6 4.0877477332889285 2 7 4.731840030501466 2 8 5.101365503099778 2 9 4.83914756616322 2 10 5.077223158110499 2 11 3.864193228575701 2 12 0.18364594894283925 2 13 1.098428890623964 2 14 4.316116469286378 2 15 5.158259420565798 2 16 4.821840019447134 2 17 7.244773450536055 2 18 5.589632503471986 2 19 6.1692908648977145 2 20 5.47610475444089 2 21 7.0191412474670365 2 22 7.9775745311767245 2 23 6.893502677107232 2 24 7.709683233622702 2 25 6.5585266967604525 2 26 4.9811405756042575 2 27 5.375954426090416 2 28 5.119398243234006 2 29 5.107209325030933 2 30 6.226805614547587 2 31 3.744448850748828 2 32 3.8686653662234605 2 33 3.7401720178277387 2 34 4.0977833080060115 2 35 3.7833880441129644 2 36 5.173397442872481 2 37 5.243002714851951 2 38 5.878742820611411 2 39 6.350597576044962 2 40 4.806749392336357 2 41 9.208351786133566 2 42 7.725276350640009 2 43 8.152888169944653 2 44 -1.9636111617468117 2 45 7.030511575907575 2 46 6.777169278194872 2 47 2.832184770105613 2 48 6.8390478623982744 2 49 -1.2869333937819363 2 50 6.8461565300065494 2 51 10.141388319357162 2 52 10.386058000412486 2 53 9.912246360845995 2 54 10.13975997186538 2 55 9.87415278052418 2 56 10.87785716842222 3 1 4.372896635468543 3 2 4.339545383966083 3 3 4.026421435977454 3 4 4.17549959084689 3 5 7.889774193745229 3 6 3.95409520250918 3 7 4.513235379338681 3 8 4.370175352620983 3 9 4.784701601730937 3 10 4.536593883144704 3 11 4.247503762091426 3 12 4.154507782048171 3 13 5.284749000063087 3 14 4.852368179272695 3 15 0.32665887337017807 3 16 6.529227160350008 3 17 7.7769211933541165 3 18 8.309741942596471 3 19 7.207184161751339 3 20 6.1503865601864405 3 21 6.910386117508079 3 22 7.44925715771571 3 23 7.20348899721667 3 24 7.659751633075505 3 25 6.39929277063186 3 26 6.683329358093999 3 27 6.081015985597936 3 28 5.826238188514441 3 29 6.449616985769648 3 30 6.7103897458745365 3 31 5.54718482886101 3 32 5.72844648797448 3 33 4.198174753805851 3 34 5.715840763546371 3 35 5.791322380638267 3 36 5.833315363529113 3 37 5.8841791854401855 3 38 7.1472790723214 3 39 7.956479928986141 3 40 8.890470322568913 3 41 11.009582976284047 3 42 10.677123135865727 3 43 9.899946993474067 3 44 10.619288975052097 3 45 9.46566889464902 3 46 11.085514011437006 3 47 11.776918012239673 3 48 11.523726384970097 3 49 12.15205429431656 3 50 10.732339840540067 3 51 11.382427991039783 3 52 10.474566892341347 3 53 10.984749780818635 3 54 11.470676524532958 3 55 11.94309942895099 3 56 11.701933775809362 4 1 4.732130561597131 4 2 4.228054275894948 4 3 4.175395444185391 4 4 4.41708645815195 4 5 7.426299501542768 4 6 4.252453615641869 4 7 4.578898854030882 4 8 4.70136550309978 4 9 4.7685593308691026 4 10 4.159576099286973 4 11 4.581840287399224 4 12 5.465998890119309 4 13 5.439605361212202 4 14 5.492587057521675 4 15 5.993553538212858 4 16 7.480663548858896 4 17 8.633008744653704 4 18 9.29551485641316 4 19 7.851643806074183 4 20 7.440810636793827 4 21 7.866200070996452 4 22 8.236398060588488 4 23 7.422914441813109 4 24 7.592036174799173 4 25 6.770291402642805 4 26 6.2164346932513155 4 27 5.964189720208061 4 28 5.237045302057535 4 29 6.754268148560346 4 30 7.1326879674887635 4 31 5.33268414486648 4 32 4.680430072105807 4 33 5.904877900180679 4 34 5.791900955064836 4 35 5.183388044112963 4 36 4.985162148754835 4 37 5.07829683249901 4 38 5.596389879434941 4 39 6.774126987809673 4 40 0.5479258629245933 4 41 8.878940021427685 4 42 8.984099880051772 4 43 8.7175940522976 4 44 9.518741779429657 4 45 7.806982164142866 4 46 10.624228101724285 4 47 10.938067123046789 4 48 10.180224332986512 4 49 10.748360723865122 4 50 10.90498005941831 4 51 11.153153025239511 4 52 9.891940353353661 4 53 10.559305184375408 4 54 10.90446585421832 4 55 10.662388074641829 4 56 11.419033639010458 5 1 4.875049951575349 5 2 5.0755488292718525 5 3 4.561161602500569 5 4 4.4801220427561645 5 5 8.223394635892806 5 6 4.433136259069613 5 7 4.894802993464431 5 8 5.009136018713434 5 9 5.0456835138756375 5 10 5.397920325866494 5 11 5.6190225677187655 5 12 5.406158657730053 5 13 6.084921265351632 5 14 5.532528960208673 5 15 7.196167917297828 5 16 7.805282285242338 5 17 9.145281802024801 5 18 9.025217107684039 5 19 8.539225505420593 5 20 7.575451159669647 5 21 7.752619824082867 5 22 8.468206339455588 5 23 7.280721268247387 5 24 8.193343364341654 5 25 7.679223864516445 5 26 6.715054882067584 5 27 6.7890263215152515 5 28 6.598991562043015 5 29 7.011058272017134 5 30 6.5310902913812825 5 31 4.977273832593422 5 32 5.575273935577123 5 33 5.140752990957736 5 34 5.33714423756302 5 35 6.033642219857331 5 36 6.654733681071459 5 37 7.2483767163043815 5 38 7.83705799853443 5 39 7.97499844750466 5 40 10.40181112073142 5 41 12.015468706975977 5 42 11.92977889239745 5 43 11.424638351498757 5 44 12.758393195551665 5 45 11.096016296314254 5 46 12.986174361709764 5 47 13.523688038079467 5 48 12.844857593698201 5 49 13.169711486392355 5 50 12.761624939592604 5 51 12.389318602581561 5 52 11.960785669257799 5 53 12.41914541676466 5 54 11.857842760536403 5 55 13.322083063748583 5 56 11.908939230876832 6 1 4.1237272002525955 6 2 4.387718141441162 6 3 3.958868833541132 6 4 4.438234917535709 6 5 7.749408745240249 6 6 3.7364872290872526 6 7 3.9080305066919436 6 8 4.457107800018548 6 9 4.680604148796277 6 10 4.392629320575487 6 11 3.9717562537857845 6 12 5.308715976954041 6 13 5.2529106833410495 6 14 4.707573051919432 6 15 5.604898076027986 6 16 6.8833246132846675 6 17 8.414241237650902 6 18 8.185710934844533 6 19 7.082876299071383 6 20 6.333387667606154 6 21 7.22572388052026 6 22 7.161328032577284 6 23 7.064230968423754 6 24 7.570327491325781 6 25 6.304885240177818 6 26 5.583661584007622 6 27 5.308867591356524 6 28 4.300490680208799 6 29 5.316172910465102 6 30 4.898374241998567 6 31 3.3868858255387444 6 32 2.7026989796688383 6 33 3.5093596928977675 6 34 4.262629246381362 6 35 4.382407651956097 6 36 3.6168148098192567 6 37 4.074655375916375 6 38 3.830003324813088 6 39 4.599337071843287 6 40 0.4472255828125462 6 41 8.733281758122363 6 42 7.357909403861299 6 43 7.530899374426447 6 44 7.368181555340023 6 45 7.394517178148469 6 46 8.997197289399356 6 47 9.472100736492166 6 48 9.304734136908078 6 49 9.406483973164843 6 50 9.47458790255557 6 51 9.513657226920184 6 52 10.033536992009122 6 53 9.57611190706448 6 54 9.241440644134286 6 55 9.505945497611016 6 56 9.236540641811576 7 1 4.669338215743132 7 2 4.490988365193317 7 3 3.91784760577292 7 4 4.29121827955521 7 5 7.827291705653327 7 6 4.232184303097576 7 7 4.0041291871279725 7 8 4.929855935417287 7 9 4.470047637034945 7 10 4.534912739967339 7 11 4.780706339844301 7 12 5.55728166829082 7 13 5.917139025280235 7 14 5.072317744977376 7 15 6.4237448918627535 7 16 7.657134137094193 7 17 8.431166079876949 7 18 8.603381617575462 7 19 7.946186683466106 7 20 6.84988221723323 7 21 7.473428986659098 7 22 7.63866595569834 7 23 7.082446688446705 7 24 7.813155948009662 7 25 7.177662061749821 7 26 5.634436110685762 7 27 6.613658182291687 7 28 5.943778115664909 7 29 6.156677787114557 7 30 5.887330065291735 7 31 4.683357426227214 7 32 4.821890029582775 7 33 5.297082010740567 7 34 3.8892787013440717 7 35 4.670560262397867 7 36 5.781760306090053 7 37 5.5649020770064475 7 38 5.575270106224448 7 39 7.287805230190962 7 40 7.416387946553225 7 41 10.186523295701246 7 42 8.668437229449367 7 43 9.255368680221054 7 44 9.515198193320519 7 45 9.429944602130114 7 46 10.812888626175031 7 47 12.247918292430203 7 48 10.757545381888 7 49 11.670827059797087 7 50 10.588041717816612 7 51 10.85875189129196 7 52 10.732762465330982 7 53 10.689709153191846 7 54 11.490149766337385 7 55 11.767419966916808 7 56 10.676156247089832 8 1 4.903802388222523 8 2 4.539973780538908 8 3 4.468893896197777 8 4 4.7590369225482405 8 5 8.179859873059794 8 6 3.909419559914319 8 7 4.089734767343572 8 8 4.455854667186465 8 9 4.393636730249909 8 10 4.54363182684115 8 11 4.789425426718111 8 12 5.6909214907385035 8 13 5.740379355020252 8 14 4.907447738636222 8 15 5.719559730163326 8 16 7.380818347620508 8 17 8.338426701310052 8 18 8.308517952388396 8 19 7.151798604835793 8 20 5.977497943295376 8 21 6.521463228891189 8 22 7.568596203003349 8 23 6.183905153887412 8 24 7.126091902353348 8 25 6.73283010233321 8 26 6.027270606564009 8 27 6.10335380689537 8 28 4.920791432088496 8 29 5.764794464349816 8 30 6.486093540244177 8 31 4.806058757869575 8 32 4.74405236312748 8 33 5.799459943524333 8 34 4.750724484476603 8 35 5.59731993265785 8 36 5.452654408816752 8 37 4.8841791854401855 8 38 5.893913099249179 8 39 7.390690455301929 8 40 3.0067493923363564 8 41 11.535286770653688 8 42 10.176205143209671 8 43 10.05784173031617 8 44 11.784221655590652 8 45 9.388251513988067 8 46 12.150079494913143 8 47 13.145033067319236 8 48 12.458242908837903 8 49 11.663376203741283 8 50 11.228973867467847 8 51 11.068168505115672 8 52 11.103085864189577 8 53 11.58175100480884 8 54 10.861741396013986 8 55 11.540406650493221 8 56 9.972129614242654 9 1 4.594423170948566 9 2 4.20090495462798 9 3 4.363630738303037 9 4 4.422667151967943 9 5 8.003071748903249 9 6 4.196194188794209 9 7 4.470301568963009 9 8 4.603627946538694 9 9 4.784999753191883 9 10 4.570284998231166 9 11 4.431463213492741 9 12 5.4469943652324275 9 13 5.750163430593801 9 14 4.7670968614432425 9 15 6.614971336101242 9 16 6.567390547350602 9 17 8.445376768786435 9 18 9.019044268177868 9 19 7.05631952251461 9 20 6.643827228045716 9 21 7.268764173560552 9 22 7.580741951991204 9 23 6.231813386006177 9 24 7.223257894256182 9 25 6.556716742009321 9 26 6.612965613311651 9 27 6.2548382873272175 9 28 5.67686430658242 9 29 6.2337553280475255 9 30 5.75093834456267 9 31 5.151537840190759 9 32 4.822662349632203 9 33 5.19250987604795 9 34 4.545596279348398 9 35 4.375997395545841 9 36 5.323774516778965 9 37 5.473922775183777 9 38 5.831834826644592 9 39 7.1103260828322945 9 40 3.6093134949004586 9 41 8.101413626254228 9 42 8.866151162103058 9 43 8.733280326807403 9 44 9.58887752603599 9 45 8.838656372287666 9 46 11.64097018317225 9 47 11.566423080814513 9 48 11.054734136908081 9 49 12.119853936534806 9 50 10.50663918460685 9 51 11.640030853293808 9 52 11.202950911423041 9 53 10.262925093877666 9 54 11.701147603841246 9 55 11.893308134973655 9 56 10.363830019100956 10 1 3.7951557716811664 10 2 3.7162895700125915 10 3 3.477916452588751 10 4 3.1263301556309457 10 5 7.613694459525963 10 6 3.479344371944393 10 7 4.217554316215754 10 8 3.833298276209021 10 9 3.785366053558178 10 10 3.840248368194537 10 11 4.3003276823572065 10 12 4.377763596001664 10 13 5.350529730960098 10 14 4.533763528109908 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3.4412739927656233 115 8 3.1435408638101023 115 9 3.7983040589490145 115 10 3.734048907278094 115 11 1.4138047713059976 115 12 -0.6618590455077715 115 13 -2.406343584403782 115 14 -4.245481754908962 115 15 -6.661274430710559 115 16 2.392983193697969 115 17 2.462309521568244 115 18 4.481308419121266 115 19 3.145095526384953 115 20 3.2190570296906102 115 21 6.364313278543616 115 22 5.825299281454189 115 23 5.47685450840579 115 24 5.264622190337459 115 25 4.69459772894691 115 26 4.501451341420022 115 27 4.76596552486955 115 28 4.3227278769742945 115 29 4.441060601390529 115 30 5.154663550174668 115 31 4.553327874056269 115 32 2.866445610396596 115 33 3.56236957609633 115 34 3.118649012778487 115 35 3.2204579664215096 115 36 3.8697348457581633 115 37 4.572858430723205 115 38 4.614369901632497 115 39 4.701762947854064 115 40 6.402975807430696 115 41 7.3972530069992715 115 42 5.8105149743913955 115 43 5.267871521775955 115 44 -3.2115578876069684 115 45 4.737059855596808 115 46 1.521231431358025 115 47 -10.090567727119693 115 48 0.45724986017851776 115 49 -3.6660676890094592 115 50 1.0013174623039944 115 51 1.889668008591343 115 52 1.6770679893137057 115 53 1.2796159501911646 115 54 0.6551872748620511 115 55 1.2375268093810057 115 56 4.0125963471125665 116 1 4.536425612951007 116 2 4.37184512556815 116 3 3.903313277985575 116 4 3.926330155630943 116 5 7.158138903970407 116 6 3.5777570703570873 116 7 4.233427332088773 116 8 4.028536371447117 116 9 4.363143831335961 116 10 4.011676939623108 116 11 3.6384229204524487 116 12 3.328557246795313 116 13 4.042593223023587 116 14 4.227414321760698 116 15 -5.184784463654555 116 16 4.57776905772911 116 17 5.549161872571535 116 18 5.566663315796919 116 19 5.332876299071383 116 20 4.257990842209324 116 21 5.523342928139304 116 22 6.68513755638681 116 23 6.250738904931691 116 24 7.494930665928958 116 25 6.404091589384166 116 26 5.718582218928255 116 27 4.804899337388264 116 28 4.272712902431021 116 29 5.395537989830185 116 30 4.989644083268409 116 31 5.128949317602238 116 32 5.0638100907799455 116 33 4.826820010358084 116 34 5.250724484476603 116 35 5.433994953543401 116 36 6.196179889184339 116 37 6.288941090202091 116 38 7.3538128486226135 116 39 8.012035484541698 116 40 10.197225582812546 116 41 9.65391667875728 116 42 8.615845911797805 116 43 4.098359691886767 116 44 1.3245307616892283 116 45 7.279437813069105 116 46 3.4694195116215774 116 47 -4.031867517476087 116 48 2.7055277877017296 116 49 -1.9149445982637268 116 50 3.3277625057301705 116 51 7.331117544380501 116 52 6.791473499945631 116 53 7.449127780080353 116 54 7.693821596515239 116 55 6.882929624595143 116 56 9.78019143546237 117 1 4.1713462478716465 117 2 4.127400681123703 117 3 3.6969640716363728 117 4 3.8628380921388796 117 5 7.196234142065645 117 6 3.6825189751189953 117 7 4.265173363834805 117 8 4.485679228589973 117 9 4.201239069431196 117 10 4.4053277332739 117 11 0.8511213331508642 117 12 -4.522236403998338 117 13 -4.811375030944664 117 14 -0.09956980522342462 117 15 -4.47049874936884 117 16 -6.365088085128029 117 17 1.8983682217778863 117 18 -2.0142890651554666 117 19 1.8281143943094769 117 20 0.975451159669646 117 21 3.7328667376631195 117 22 4.986724857974107 117 23 3.3745484287412175 117 24 5.1711211421194285 117 25 4.975520160812742 117 26 5.636042536388572 117 27 5.300137432626364 117 28 4.8409668706849835 117 29 5.443157037449229 117 30 5.21504090866523 117 31 4.113076301729226 117 32 4.565397392367245 117 33 4.106185089723162 117 34 4.006280040032156 117 35 4.732407651956098 117 36 6.3930052860097355 117 37 6.917512518773517 117 38 7.728416023225787 117 39 8.478702151208367 117 40 9.940082725669686 117 41 9.247567472408075 117 42 7.717433213385107 117 43 -6.177830784303712 117 44 -0.3675327303742648 117 45 7.298485432116724 117 46 0.6503718925739577 117 47 -1.7874230730316434 117 48 0.3880674702414133 117 49 -1.2228811062002372 117 50 3.391254569222234 117 51 8.026355639618597 117 52 7.353378261850391 117 53 7.01420714515972 117 54 8.643027945721588 117 55 8.159120100785618 117 56 10.192889848160782 118 1 3.545329987709046 118 2 3.377265179768692 118 3 2.8514356163518144 118 4 3.2747622113800716 118 5 6.943659616320385 118 6 3.178995939888644 118 7 3.46842539635513 118 8 3.170232074118431 118 9 3.5226482835233384 118 10 2.973349413490702 118 11 -0.6832960110225841 118 12 -8.151504696681265 118 13 -7.951212429318648 118 14 -9.175992569451068 118 15 -9.530119345574803 118 16 -7.903841472661906 118 17 -0.6978377402817344 118 18 -7.017541097675789 118 19 -0.6938368252027161 118 20 -0.8771233660756117 118 21 2.861322022215962 118 22 4.810573096456491 118 23 4.0889115723726555 118 24 4.5375168060760736 118 25 3.571726122872363 118 26 5.296205138014592 118 27 5.035638787639911 118 28 4.05397500076629 118 29 5.432316929048145 118 30 5.010162859884744 118 31 4.584621017176378 118 32 4.151305251445841 118 33 5.00645609243319 118 34 4.73852936252538 118 35 5.0267165950455315 118 36 3.9995093510503814 118 37 4.237837722025549 118 38 4.241153150597061 118 39 5.529650660693456 118 40 7.494554270385134 118 41 6.626429261025962 118 42 5.9011730507834805 118 43 1.5462884568886999 118 44 0.26336157856882636 118 45 5.454040987672279 118 46 0.48993828823791574 118 47 -0.11587835758448861 118 48 1.7701812913796218 118 49 -0.7047239246284214 118 50 1.4969456261328027 118 51 6.773239108453286 118 52 7.241725145319226 118 53 5.586564868736954 118 54 6.622973745179582 118 55 7.193808447669088 118 56 9.50454296469195 119 1 3.563503110616743 119 2 3.3849170209929866 119 3 3.0891209343814694 119 4 3.228851164034303 119 5 6.069436756444727 119 6 3.1034340077987324 119 7 3.500467481481863 119 8 3.454306679570365 119 9 3.5097358014573388 119 10 3.053693746345793 119 11 0.3190951893600129 119 12 -4.3810599334101035 119 13 -1.662355423101527 119 14 -3.938785491497935 119 15 -6.496642540218513 119 16 -7.656591353101884 119 17 1.3232048230850744 119 18 -6.167230241626054 119 19 -0.3365914880434637 119 20 0.24865377404873001 119 21 3.999533404329785 119 22 4.914829433137509 119 23 3.4935026771072266 119 24 2.846938135583482 119 25 4.138918853623196 119 26 4.526238614819945 119 27 4.579875994717867 119 28 4.044888439312441 119 29 4.613091677972111 119 30 4.83464875180249 119 31 3.7405272821213806 119 32 3.6333712485763954 119 33 3.3440935864551946 119 34 3.015430366829545 119 35 3.661819416661981 119 36 3.220456266401893 119 37 3.599865459949992 119 38 4.619919291199647 119 39 3.7505975760449637 119 40 5.987141549199102 119 41 4.114234139074743 119 42 3.427237134953735 119 43 -5.3843667320161295 119 44 -3.5008660637075977 119 45 3.9638449092409083 119 46 -1.6267522904325773 119 47 -2.175658367149289 119 48 -5.435461941523293 119 49 -4.337913785938799 119 50 -0.7656081758758049 119 51 3.615898123278729 119 52 2.1546854513928757 119 53 2.2651875373165815 119 54 1.4221129130418504 119 55 3.3525841530732023 119 56 5.532759129206536 120 1 3.497587382623415 120 2 3.3482044636296138 120 3 2.9593754191540995 120 4 3.3427435294911234 120 5 6.200016647975811 120 6 3.1255449798471275 120 7 3.612691094331254 120 8 3.32539554064671 120 9 2.9865818590293074 120 10 3.646935298285719 120 11 -0.6604625911990212 120 12 -5.7264917231472765 120 13 -4.558892761441118 120 14 -5.310917322953923 120 15 -0.1891748723002813 120 16 -6.066270118224956 120 17 1.3073516733145283 120 18 -4.949040838205111 120 19 0.2366250326073498 120 20 0.03880813366491509 120 21 3.261944751847512 120 22 5.669939987997747 120 23 3.8927517384338906 120 24 4.266629416351112 120 25 3.8431324539569474 120 26 5.050226933551691 120 27 4.096354926716195 120 28 4.354442047989952 120 29 5.277672403879489 120 30 4.623551546963107 120 31 4.332934457757592 120 32 4.065160985747866 120 33 4.128407311945388 120 34 3.484767037668089 120 35 2.5721239640128353 120 36 3.3731471299813656 120 37 4.57566854714231 120 38 3.5260519570319353 120 39 5.520309716220183 120 40 4.857813222123589 120 41 -5.97087224390398 120 42 -3.160580971012056 120 43 -7.7489891767387 120 44 -15.727816418317525 120 45 -4.865107948497933 120 46 -7.407311322556065 120 47 -4.4313947042373165 120 48 -5.2360460049358934 120 49 -14.661651791779432 120 50 -5.048461742834501 120 51 -0.2781360861497255 120 52 -1.172390059662611 120 53 0.08371069125901087 120 54 -0.9120547965951964 120 55 0.10143688565559472 120 56 3.620785829248252 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 1230.0 5 66.0 6 177.0 7 130.0 8 220.0 9 302.0 10 244.0 11 210.0 12 188.0 13 157.0 14 211.0 15 241.0 16 275.0 17 335.0 18 398.0 19 525.0 20 682.0 21 919.0 22 1054.0 23 1198.0 24 1521.0 25 1643.0 26 1696.0 27 1754.0 28 1827.0 29 2064.0 30 2168.0 31 2537.0 32 2847.0 33 3122.0 34 3444.0 35 3632.0 36 3945.0 37 4544.0 38 4506.0 39 3931.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 0.0 0.0 0.0 100.0 3 0.0 0.0 0.0 100.0 4 0.0 0.0 100.0 0.0 5 0.0 100.0 0.0 0.0 6 100.0 0.0 0.0 0.0 7 99.52915878841804 0.3948392837281726 0.050050050050050046 0.025951877803729657 8 99.18993067141216 0.5023542060579098 0.15571126682237793 0.1520038557075594 9 95.88477366255144 2.150298446594743 1.22900678456234 0.7359211062914767 10 51.909316724131536 25.460645831016198 14.384755125495866 8.245282319356393 11 29.514699885070257 29.099469840210578 17.00960219478738 24.376228079931785 12 27.92051310569829 24.643161680198716 22.668965261557855 24.76735995254514 13 22.95258221184147 19.788306825343863 22.567011455900346 34.69209950691432 14 19.728988247506766 29.599970340711078 24.921217513810106 25.749823897972046 15 28.45654075990841 20.963568330323735 23.096971163691197 27.48291974607666 16 25.14423522864298 28.542806789722658 24.48752434839069 21.82543363324367 17 25.77457215433964 24.28197949307474 29.149129475460295 20.794318877125324 18 25.83880176472769 22.162903644385125 22.211099988877766 29.787194602009414 19 26.423246671364463 24.38526870155398 21.453473278196046 27.73801134888551 20 26.569662775375363 25.07748557005252 27.81499972160873 20.537851932963385 21 25.675675675675674 22.726430133837543 24.11114818522226 27.48674600526452 22 24.26315204092982 22.744967189411632 29.108738367997628 23.88314240166092 23 23.221369517665813 24.98980461943425 27.453379305231156 24.33544655766878 24 22.239317823709335 25.713226434331265 23.36824543516545 28.679210306793955 25 23.73280433089844 26.133709073380544 26.573102450962217 23.560384144758796 26 30.27599510331268 26.23808287272323 20.25818896761509 23.227733056349003 27 24.163713612497446 30.375331638805914 20.282380007050225 25.178574741646415 28 27.24121310989174 30.996959810173514 19.766795195017053 21.995031884917694 29 30.186402287326874 28.036463555010954 20.218335746908767 21.558798410753408 30 26.712417694519154 25.913011221366965 26.306222758045074 21.06834832606881 31 28.406415129322333 30.249374246778533 20.934458144062297 20.40975247983684 32 30.47299427066916 28.431572506628594 20.497654497246582 20.597778725455658 33 29.307944991926956 27.888202215912255 20.672568342519902 22.13128444964089 34 23.46233843411083 27.094785321646114 28.758728272173528 20.684147972069532 35 22.826591419607553 27.86678300258043 30.272708708485712 19.033916869326305 36 26.372505378737294 27.804362341419985 24.374953631575043 21.448178648267678 37 27.267495823278264 27.133840727677743 25.069612028958606 20.52905142008539 38 28.75264663274024 26.94364993870956 24.467887522751756 19.835815905798448 39 27.315932127872873 26.757138083392125 21.650020421044815 24.276909367690195 40 22.86605874878541 20.73772329770536 23.962926975110246 32.43329097839898 41 19.830702099368878 19.02166788396352 21.39071483416671 39.75691518250089 42 23.213190326598138 28.16513932003643 23.720653568042827 24.901016785322604 43 20.058366482025356 24.978623740659504 26.638536748578012 28.32447302873713 44 20.51892938242192 26.019059673156384 27.03904999441611 26.422960950005585 45 24.60103665446709 26.044550132832967 27.629256692737847 21.725156519962102 46 21.18828451882845 27.923756392375637 24.851696885169687 26.03626220362622 47 19.733606557377048 25.847615499254843 22.96199701937407 31.45678092399404 48 24.848361187883732 26.335998219287344 24.850216096900446 23.965424495928474 49 20.67120080276508 26.807150555617497 24.803954361318617 27.717694280298804 50 21.49622970914899 28.982950113294454 25.251662271089486 24.269157906467072 51 21.017604521034336 29.799416280928746 24.940047961630697 24.242931236406225 52 21.26885075403016 30.907807740881065 26.30376643637174 21.519575068717035 53 21.50597594750832 29.8221156527073 26.712392423651 21.959515976133385 54 20.967831855940236 27.275092453215887 28.118786121796656 23.63828956904722 55 20.96369416095031 25.6799211793356 28.59851653560872 24.757868124105368 56 22.06098218101414 26.271391144391387 28.688753042605768 22.9788736319887 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 1.0 7 2.0 8 3.0 9 4.0 10 42.0 11 80.0 12 263.5 13 452.5 14 458.0 15 385.0 16 312.0 17 266.5 18 221.0 19 223.0 20 225.0 21 216.0 22 230.0 23 253.0 24 255.0 25 257.0 26 256.5 27 256.0 28 299.5 29 349.0 30 355.0 31 400.5 32 446.0 33 475.5 34 505.0 35 569.5 36 634.0 37 793.0 38 973.0 39 994.0 40 1035.5 41 1077.0 42 1168.0 43 1259.0 44 1356.5 45 1454.0 46 1748.0 47 2650.0 48 3258.0 49 3443.5 50 3629.0 51 3232.5 52 2836.0 53 2832.5 54 2840.0 55 2851.0 56 2909.0 57 2967.0 58 2991.5 59 3016.0 60 3196.0 61 3376.0 62 3411.5 63 3509.0 64 3571.0 65 3178.5 66 2786.0 67 2439.0 68 2092.0 69 1805.0 70 1518.0 71 1292.0 72 932.5 73 799.0 74 643.0 75 487.0 76 405.0 77 323.0 78 248.5 79 126.5 80 79.0 81 58.0 82 37.0 83 26.0 84 15.0 85 9.5 86 4.0 87 2.5 88 1.0 89 1.0 90 0.5 91 0.0 92 0.5 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.4244985726467208 16 0.0760019278537797 17 0.024098172246320396 18 0.0 19 0.03707411114818522 20 0.12049086123160196 21 0.0 22 0.0 23 0.0 24 0.0018537055574092613 25 0.01482964445927409 26 0.05931857783709636 27 0.08712416119823527 28 0.0037074111148185226 29 0.15571126682237793 30 0.05746487227968709 31 0.020390761131501872 32 0.024098172246320396 33 0.11678345011678344 34 0.18166314462610758 35 0.14644273903533161 36 0.05561116672227783 37 0.14088162236310384 38 0.19278537797056317 39 0.14829644459274088 40 0.7970933896859822 41 1.0176843510176843 42 0.2762021280539799 43 0.2743484224965706 44 0.4078152226300374 45 0.22059096133170206 46 0.31698365031698367 47 0.49308567827086347 48 0.06487969450932414 49 0.24468913357802244 50 0.19278537797056317 51 0.28361695028361694 52 0.18907796685574463 53 0.2724947169391614 54 0.2502502502502503 55 0.28361695028361694 56 0.23542060579097618 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 53946.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.34215697178661 #Duplication Level Percentage of deduplicated Percentage of total 1 89.64599860987944 69.3341489637786 2 6.523979579608369 10.091573054536017 3 1.6921122642187763 3.926148370592815 4 0.7741533446779953 2.3949875801727654 5 0.41703616710207797 1.612723834946057 6 0.2181051218752247 1.0121232343454567 7 0.11744121947127484 0.6358210061913765 8 0.12942501737650697 0.8008008008008007 9 0.0766963065934856 0.5338672005338673 >10 0.36191069673801024 4.858562265969674 >50 0.023967595810464253 1.3253994735476216 >100 0.0191740766483714 3.4738442145849557 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCCTAGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC 410 0.760019278537797 No Hit GCCTAGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT 380 0.7044081118155192 No Hit GCCTAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 289 0.5357209060912764 No Hit GCCTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 283 0.5245986727468209 No Hit GCCTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA 155 0.2873243613984355 No Hit GCCTAGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC 150 0.2780558336113892 No Hit GCCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 106 0.1964927890853817 No Hit GCCTAGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA 101 0.18722426129833536 No Hit GCCTAGGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT 93 0.1723946168390613 No Hit GCCTAGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT 93 0.1723946168390613 No Hit GCCTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 92 0.17054091128165202 No Hit GCCTAGGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT 74 0.1371742112482853 No Hit GCCTAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 70 0.12975938901864828 No Hit GCCTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAAA 69 0.12790568346123904 No Hit GCCTAGGGGACTAATCCTAGCCCTAGCCCTACACAAATATCATTATACTATTATAT 57 0.10566121677232788 No Hit GCCTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 57 0.10566121677232788 No Hit GCCTAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 56 0.10380751121491863 No Hit GCCTAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 54 0.10010010010010009 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.020390761131501872 0.0 0.0 0.0 0.0 6 0.020390761131501872 0.0 0.0 0.0 0.0 7 0.020390761131501872 0.0 0.0 0.0 0.0 8 0.020390761131501872 0.0 0.0 0.0 0.0 9 0.020390761131501872 0.0 0.0 0.0 0.0 10 0.031512994475957436 0.0 0.0 0.0 0.0 11 0.0333667000333667 0.0 0.0 0.0 0.0 12 0.03892781670559448 0.0 0.0 0.0 0.0 13 0.03892781670559448 0.0 0.0 0.0 0.0 14 0.059318577837096355 0.0 0.0 0.0 0.0 15 0.06302598895191487 0.0 0.0 0.0 0.0 16 0.06487969450932414 0.0 0.0 0.0 0.0 17 0.0667334000667334 0.0 0.0 0.0 0.0 18 0.07044081118155192 0.0 0.0 0.0 0.0 19 0.07414822229637044 0.0 0.0 0.0 0.0 20 0.07600192785377971 0.0 0.0 0.0 0.0 21 0.07785563341118897 0.0 0.0 0.0 0.0 22 0.08527045564082601 0.0 0.0 0.0 0.0 23 0.09268527787046306 0.0 0.0 0.0 0.0 24 0.09639268898528158 0.0 0.0 0.0 0.0 25 0.10195380565750936 0.0 0.0 0.0 0.0 26 0.10936862788714641 0.0 0.0 0.0 0.0 27 0.11678345011678345 0.0 0.0 0.0 0.0 28 0.12605197790382974 0.0 0.0 0.0 0.0 29 0.1334668001334668 0.0 0.0 0.0 0.0 30 0.1390279168056946 0.0 0.0 0.0 0.0 31 0.14458903347792237 0.0 0.0 0.0 0.0 32 0.15200385570755942 0.0 0.0 0.001853705557409261 0.0 33 0.15385756126496866 0.0 0.0 0.001853705557409261 0.0 34 0.15941867793719647 0.0 0.0 0.001853705557409261 0.0 35 0.1612723834946057 0.0 0.0 0.001853705557409261 0.0 36 0.16312608905201498 0.0 0.0 0.001853705557409261 0.0 37 0.1668335001668335 0.0 0.0 0.001853705557409261 0.0 38 0.17054091128165202 0.0 0.0 0.001853705557409261 0.0 39 0.17054091128165202 0.0 0.0 0.001853705557409261 0.0 40 0.1761020279538798 0.0 0.0 0.001853705557409261 0.0 41 0.18166314462610758 0.0 0.0 0.001853705557409261 0.0 42 0.1909316724131539 0.0 0.0 0.001853705557409261 0.0 43 0.19834649464279094 0.0 0.0 0.001853705557409261 0.0 44 0.2094687279872465 0.0 0.0 0.001853705557409261 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCAATC 30 9.692649E-7 50.43503 40 TATAATT 15 0.008104751 50.43503 38 TGTCCAA 30 9.692649E-7 50.43503 38 GCGCATT 35 4.821777E-8 50.435024 38 CAGGTAT 15 0.008165333 50.34023 44 GTCAGGT 15 0.008165333 50.34023 42 ACCAACA 20 4.0112468E-4 50.340225 42 ATCCTAG 15 0.008256837 50.198692 14 TTACCGA 15 0.008256837 50.198692 49 TATTATA 20 4.067466E-4 50.19869 49 AGAGCGC 15 0.008318262 50.104774 35 TGGCTAC 20 4.1052917E-4 50.10477 35 TAGAGCG 15 0.008349102 50.057945 34 ACTAATC 20 4.1243093E-4 50.057945 10 GACTCAC 15 0.008349102 50.057945 10 ATGGGCC 15 0.008380027 50.011208 50 GGACAAT 15 0.008380027 50.011208 9 TACCGAA 15 0.008380027 50.011208 50 GGCTTAC 15 0.008473312 49.87151 27 AACCACC 15 0.008504577 49.825115 17 >>END_MODULE