FastQCFastQC Report
Fri 17 Jun 2016
SRR1525921_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525921_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2698
Sequences flagged as poor quality0
Sequence length40
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.18532246108228317No Hit
GCCTAGGGGGCCGAATAGCCTCTCCACCCAAGCGGCCCGA50.18532246108228317No Hit
GCCTAGGGGCCATCTCATCCCTGCGTGTCTCCGACTCCGA40.14825796886582654No Hit
GCCTAGGGGTAGAGATGTGGAGGAATACCGCAGGGATGAG40.14825796886582654No Hit
GCCTAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG40.14825796886582654No Hit
GCCTAGGGGTCGAGAGTCGATTTTTTTTTTTTTTTTTTTT40.14825796886582654No Hit
GCCTAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT40.14825796886582654No Hit
GCCTAGGGGGTCTCTTTTCCTCTGGCGCGGTGAGTGAGTT40.14825796886582654No Hit
GCCTAGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT30.1111934766493699No Hit
GCCTAGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.1111934766493699No Hit
GCCTAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCC30.1111934766493699No Hit
GCCTAGGGGGATCCAGTGACAGATACCATTAGCTTCAAGA30.1111934766493699No Hit
GCCTAGGGGAGCAGTGTATCCGCAGAGTCGACTTTTCTTT30.1111934766493699No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGCA251.2963581E-434.921575
TAGGGGG1000.034.921574
AGGGGGT200.0020179734.9215665
AGGGGGG308.205247E-634.9215665
AGGGGGC200.0020179734.9215665
AGGGGGA308.205247E-634.9215665
TAGGGGT200.0020179734.9215664
CTAGGGG2700.031.7129653
TAGGGGA850.030.8131484
CCTAGGG2950.030.1864412
GCCTAGG3000.029.683331
AGGGGAA607.912604E-1029.1013075
TAGGGGC607.912604E-1029.1013074
Produced by FastQC (version 0.11.5)