##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1525921_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2698
Sequences flagged as poor quality	0
Sequence length	40
%GC	52
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.77761304670126	30.0	25.0	32.0	4.0	33.0
2	11.877316530763528	11.0	10.0	12.0	4.0	20.0
3	17.680133432171978	17.0	10.0	26.0	4.0	32.0
4	21.475908080059302	25.0	13.0	30.0	4.0	33.0
5	19.233876945885843	21.0	11.0	26.0	4.0	31.0
6	23.166790214974053	26.0	17.0	31.0	4.0	34.0
7	21.458487768717568	24.0	13.0	30.0	4.0	33.0
8	22.318383988139363	27.0	14.0	31.0	4.0	33.0
9	21.12416604892513	24.0	12.0	30.0	4.0	33.0
10	18.757598220904374	20.0	10.0	27.0	4.0	32.0
11	23.55263157894737	28.0	13.0	34.0	4.0	38.0
12	23.38954781319496	27.0	13.0	34.0	4.0	37.0
13	24.768346923647147	30.0	16.0	35.0	4.0	38.0
14	24.798739807264642	30.0	15.0	35.0	4.0	38.0
15	23.093773165307635	27.0	11.0	35.0	4.0	37.0
16	20.911045218680503	24.0	4.0	33.0	4.0	36.0
17	21.65418828762046	26.0	4.0	33.0	4.0	36.0
18	21.442920681986656	26.0	4.0	33.0	4.0	36.0
19	18.44736842105263	18.0	4.0	30.0	4.0	35.0
20	19.91141586360267	23.0	4.0	32.0	4.0	35.0
21	17.398072646404746	16.0	4.0	30.0	4.0	35.0
22	17.27946627131208	16.0	4.0	30.0	4.0	34.0
23	17.02853965900667	16.0	4.0	30.0	4.0	34.0
24	16.67939214232765	15.0	4.0	30.0	4.0	34.0
25	16.071163825055596	11.0	4.0	29.0	4.0	34.0
26	13.958117123795404	4.0	4.0	26.0	4.0	33.0
27	13.835063009636768	4.0	4.0	26.0	4.0	33.0
28	13.60303928836175	4.0	4.0	26.0	4.0	33.0
29	13.080059303187547	4.0	4.0	25.0	4.0	33.0
30	12.285025945144552	4.0	4.0	23.0	4.0	32.0
31	12.02186805040771	4.0	4.0	23.0	4.0	33.0
32	11.670126019273535	4.0	4.0	22.0	4.0	32.0
33	11.172720533728688	4.0	4.0	19.0	4.0	31.0
34	10.693106004447738	4.0	4.0	17.0	4.0	31.0
35	10.187916975537435	4.0	4.0	15.0	4.0	30.0
36	10.101927353595256	4.0	4.0	13.0	4.0	32.0
37	9.972572275759822	4.0	4.0	9.0	4.0	32.0
38	9.76538176426983	4.0	4.0	4.0	4.0	32.0
39	9.288361749444032	4.0	4.0	4.0	4.0	31.0
40	9.227575982209043	4.0	4.0	4.0	4.0	31.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	8.111995701058195
1	2	11.183184523809523
1	3	-2.46630291005291
1	4	5.6863177910052904
1	5	7.94298941798942
1	6	3.2171544312169296
1	7	5.241426917989415
1	8	0.347445436507936
1	9	-0.30401785714285623
1	10	6.629885912698413
1	11	-7.2566798941798965
1	12	-1.901066468253962
1	13	-2.70954034391535
1	14	-2.260548941798941
1	15	-6.247544642857147
1	16	-8.277843915343912
1	17	-4.5769014550264515
1	18	-2.107283399470905
1	19	-4.585309193121695
1	20	0.053430886243386766
1	21	10.01914682539683
1	22	8.658713624338624
1	23	6.099528769841271
1	24	1.6714616402116373
1	25	8.722089947089946
1	26	4.997767857142859
1	27	-0.3318617724867696
1	28	1.2838128306878342
1	29	5.790649801587298
1	30	6.784069113756615
1	31	-0.865550595238096
1	32	-0.3483465608465597
1	33	2.1808366402116395
1	34	3.038227513227513
1	35	-3.321271494708995
1	36	-3.318096891534392
1	37	2.098826058201057
1	38	-3.1182126322751316
1	39	-2.1304398148148156
1	40	-2.6927496693121693
4	1	-20.638004298941805
4	2	-7.066815476190477
4	3	-12.46630291005291
4	4	-16.31368220899471
4	5	-14.05701058201058
4	6	-17.53284556878307
4	7	-16.258573082010585
4	8	-17.152554563492064
4	9	-16.304017857142856
4	10	-13.870114087301587
4	11	-18.506679894179896
4	12	-18.151066468253962
4	13	-18.95954034391535
4	14	-19.51054894179894
4	15	-18.247544642857147
4	16	-15.527843915343912
4	17	-16.82690145502645
4	18	-16.857283399470905
4	19	-14.085309193121695
4	20	-14.946569113756613
4	21	-11.73085317460317
4	22	-11.841286375661376
4	23	-11.650471230158729
4	24	-11.828538359788363
4	25	-11.277910052910054
4	26	-10.00223214285714
4	27	-9.58186177248677
4	28	-9.466187169312166
4	29	-9.209350198412702
4	30	-8.215930886243385
4	31	-8.365550595238096
4	32	-7.09834656084656
4	33	-7.3191633597883605
4	34	-7.211772486772487
4	35	-6.571271494708995
4	36	-6.568096891534392
4	37	-6.401173941798943
4	38	-6.368212632275132
4	39	-5.380439814814816
4	40	-5.942749669312169
5	1	-5.304670965608473
5	2	-0.40014880952381127
5	3	-6.799636243386244
5	4	-2.6470155423280417
5	5	2.276322751322752
5	6	-10.53284556878307
5	7	-10.925239748677251
5	8	-12.485887896825398
5	9	-10.970684523809522
5	10	-5.203447420634921
5	11	-6.84001322751323
5	12	-13.817733134920628
5	13	-10.95954034391535
5	14	-4.177215608465609
5	15	-10.247544642857147
5	16	1.1388227513227562
5	17	-4.493568121693119
5	18	-3.857283399470905
5	19	3.248024140211637
5	20	5.053430886243387
5	21	5.602480158730161
5	22	8.158713624338624
5	23	7.016195436507939
5	24	5.5047949735449695
5	25	5.7220899470899464
5	26	7.664434523809527
5	27	3.7514715608465625
5	28	-1.4661871693121658
5	29	-4.876016865079368
5	30	-1.8825975529100507
5	31	-4.032217261904762
5	32	-3.0983465608465597
5	33	-3.9858300264550275
5	34	-1.8784391534391531
5	35	-4.571271494708995
5	36	1.7652364417989421
5	37	3.2654927248677232
5	38	3.2984540343915345
5	39	-2.0471064814814826
5	40	3.7239169973544968
6	1	-1.638004298941805
6	2	-0.06681547619047734
6	3	0.5336970899470899
6	4	8.68631779100529
6	5	3.94298941798942
6	6	-1.5328455687830704
6	7	-9.258573082010585
6	8	-11.152554563492064
6	9	-8.304017857142856
6	10	2.1298859126984127
6	11	13.493320105820104
6	12	11.848933531746038
6	13	13.04045965608465
6	14	-5.510548941798941
6	15	13.752455357142853
6	16	5.472156084656088
6	17	9.173098544973548
6	18	13.142716600529095
6	19	6.914690806878305
6	20	15.053430886243387
6	21	-0.7308531746031708
6	22	17.158713624338624
6	23	14.349528769841271
6	24	19.17146164021164
6	25	-0.27791005291005355
6	26	20.99776785714286
6	27	19.418138227513232
6	28	21.533812830687836
6	29	4.790649801587298
6	30	15.784069113756615
6	31	18.634449404761902
6	32	21.901653439153442
6	33	1.6808366402116395
6	34	1.788227513227513
6	35	16.428728505291005
6	36	4.431903108465608
6	37	4.598826058201057
6	38	24.63178736772487
6	39	5.619560185185184
6	40	5.057250330687831
7	1	5.961995701058196
7	2	1.133184523809522
7	3	5.933697089947088
7	4	8.086317791005289
7	5	3.3429894179894184
7	6	1.6671544312169289
7	7	3.5414269179894156
7	8	-1.152554563492064
7	9	0.6959821428571438
7	10	5.329885912698412
7	11	9.293320105820104
7	12	11.248933531746037
7	13	10.440459656084649
7	14	7.689451058201058
7	15	9.952455357142856
7	16	9.272156084656089
7	17	9.173098544973548
7	18	9.742716600529096
7	19	5.514690806878306
7	20	0.25343088624338606
7	21	13.26914682539683
7	22	5.958713624338625
7	23	6.54952876984127
7	24	10.971461640211638
7	25	4.522089947089947
7	26	-1.402232142857141
7	27	-0.98186177248677
7	28	1.7338128306878335
7	29	-3.6093501984127023
7	30	0.18406911375661572
7	31	-0.36555059523809597
7	32	3.701653439153441
7	33	-4.3191633597883605
7	34	-4.811772486772487
7	35	-1.1712714947089946
7	36	-6.568096891534392
7	37	-6.401173941798943
7	38	-6.368212632275132
7	39	-5.380439814814816
7	40	-5.942749669312169
9	1	9.861995701058195
9	2	6.433184523809523
9	3	13.78369708994709
9	4	9.68631779100529
9	5	9.94298941798942
9	6	10.46715443121693
9	7	14.491426917989415
9	8	12.847445436507936
9	9	10.445982142857144
9	10	9.879885912698413
9	11	11.243320105820104
9	12	8.848933531746038
9	13	7.54045965608465
9	14	6.239451058201059
9	15	6.752455357142853
9	16	6.222156084656088
9	17	7.1730985449735485
9	18	10.142716600529095
9	19	16.164690806878305
9	20	9.053430886243387
9	21	0.2691468253968292
9	22	0.15871362433862402
9	23	3.349528769841271
9	24	3.1714616402116373
9	25	4.9720899470899464
9	26	-4.002232142857141
9	27	-3.3318617724867696
9	28	-4.966187169312166
9	29	-2.959350198412702
9	30	-2.2159308862433846
9	31	-0.615550595238096
9	32	-0.09834656084655968
9	33	0.4308366402116395
9	34	-2.711772486772487
9	35	-3.071271494708995
9	36	-2.318096891534392
9	37	-0.40117394179894283
9	38	-0.6182126322751316
9	39	-0.1304398148148156
9	40	-1.1927496693121693
10	1	1.917551256613752
10	2	-1.7334821428571434
10	3	-4.244080687830689
10	4	1.1307622354497333
10	5	-2.6125661375661355
10	6	0.35604332010581885
10	7	-0.14746197089947444
10	8	0.847445436507936
10	9	0.3626488095238116
10	10	-2.5367807539682534
10	11	4.715542328042325
10	12	4.848933531746038
10	13	4.484904100529093
10	14	5.378339947089948
10	15	7.0857886904761855
10	16	8.583267195767199
10	17	6.950876322751327
10	18	7.9204943783068735
10	19	10.359135251322748
10	20	9.49787533068783
10	21	11.26914682539683
10	22	8.603158068783067
10	23	12.016195436507939
10	24	10.61590608465608
10	25	10.610978835978836
10	26	8.99776785714286
10	27	9.529249338624341
10	28	4.644923941798945
10	29	6.12398313492063
10	30	-1.6603753306878293
10	31	-1.365550595238096
10	32	-0.8761243386243383
10	33	-0.2080522486772498
10	34	1.2326719576719576
10	35	0.2065062830687836
10	36	-1.1236524470899472
10	37	-0.5122850529100536
10	38	-0.14599041005291014
10	39	-1.8248842592592602
10	40	-0.16497189153439074
11	1	7.361995701058195
11	2	14.933184523809523
11	3	14.53369708994709
11	4	0.6863177910052904
11	5	2.94298941798942
11	6	13.46715443121693
11	7	14.741426917989415
11	8	13.847445436507936
11	9	14.695982142857144
11	10	12.129885912698413
11	11	15.493320105820104
11	12	15.848933531746038
11	13	15.04045965608465
11	14	14.489451058201059
11	15	13.752455357142853
11	16	10.472156084656088
11	17	12.173098544973548
11	18	14.142716600529095
11	19	11.914690806878305
11	20	11.053430886243387
11	21	15.26914682539683
11	22	-2.841286375661376
11	23	12.349528769841271
11	24	6.171461640211637
11	25	11.722089947089946
11	26	18.99776785714286
11	27	11.41813822751323
11	28	14.533812830687834
11	29	13.790649801587298
11	30	13.784069113756615
11	31	11.634449404761904
11	32	-4.09834656084656
11	33	17.680836640211638
11	34	18.78822751322751
11	35	-6.571271494708995
11	36	-6.568096891534392
11	37	-6.401173941798943
11	38	-6.368212632275132
11	39	-5.380439814814816
11	40	-5.942749669312169
12	1	0.695329034391527
12	2	-0.9001488095238113
12	3	-1.9663029100529101
12	4	3.5196511243386226
12	5	3.276322751322752
12	6	3.9671544312169296
12	7	-1.925239748677253
12	8	2.514112103174604
12	9	-3.470684523809524
12	10	2.6298859126984127
12	11	4.326653439153436
12	12	2.848933531746038
12	13	5.373792989417982
12	14	5.822784391534391
12	15	7.752455357142853
12	16	8.972156084656088
12	17	5.6730985449735485
12	18	9.309383267195763
12	19	4.414690806878305
12	20	7.553430886243387
12	21	9.26914682539683
12	22	3.325380291005292
12	23	4.516195436507939
12	24	7.838128306878305
12	25	8.055423280423279
12	26	-4.3355654761904745
12	27	-2.9151951058201035
12	28	-1.1328538359788318
12	29	-5.042683531746036
12	30	0.6174024470899493
12	31	-5.532217261904763
12	32	-6.09834656084656
12	33	-2.9858300264550266
12	34	-3.211772486772487
12	35	-2.571271494708995
12	36	-3.901430224867725
12	37	-1.067840608465609
12	38	-4.868212632275132
12	39	-0.8804398148148156
12	40	-5.942749669312169
13	1	2.0286623677248627
13	2	-0.06681547619047734
13	3	0.867030423280422
13	4	7.6863177910052904
13	5	-1.3903439153439123
13	6	-1.1995122354497383
13	7	-6.258573082010585
13	8	3.514112103174604
13	9	1.6959821428571438
13	10	1.7965525793650805
13	11	12.82665343915344
13	12	7.515600198412706
13	13	9.373792989417986
13	14	7.489451058201059
13	15	11.085788690476189
13	16	8.80548941798942
13	17	14.173098544973548
13	18	11.47604993386243
13	19	9.914690806878305
13	20	15.053430886243387
13	21	11.602480158730161
13	22	9.825380291005292
13	23	18.016195436507935
13	24	11.838128306878305
13	25	17.388756613756613
13	26	8.331101190476192
13	27	9.084804894179898
13	28	4.533812830687834
13	29	6.790649801587298
13	30	-1.2159308862433846
13	31	0.3011160714285701
13	32	-7.09834656084656
13	33	-7.3191633597883605
13	34	-7.211772486772487
13	35	-6.571271494708995
13	36	-6.568096891534392
13	37	-6.401173941798943
13	38	-6.368212632275132
13	39	-5.380439814814816
13	40	-5.942749669312169
15	1	9.561995701058198
15	2	4.933184523809523
15	3	14.53369708994709
15	4	10.68631779100529
15	5	10.342989417989418
15	6	11.667154431216932
15	7	9.541426917989416
15	8	10.447445436507937
15	9	10.895982142857143
15	10	11.929885912698413
15	11	12.2933201058201
15	12	10.44893353174604
15	13	12.240459656084653
15	14	11.689451058201062
15	15	11.952455357142856
15	16	10.272156084656089
15	17	12.173098544973548
15	18	10.542716600529094
15	19	12.714690806878306
15	20	16.453430886243385
15	21	-2.93085317460317
15	22	-1.6412863756613767
15	23	0.9495287698412724
15	24	-0.8285383597883627
15	25	0.5220899470899472
15	26	-0.6022321428571402
15	27	-3.5818617724867696
15	28	-0.8661871693121661
15	29	3.3906498015872995
15	30	-2.615930886243385
15	31	0.43444940476190474
15	32	4.90165343915344
15	33	4.6808366402116395
15	34	-1.4117724867724863
15	35	-1.7712714947089943
15	36	-0.5680968915343918
15	37	-0.0011739417989424794
15	38	-0.7682126322751319
15	39	-0.9804398148148152
15	40	-0.34274966931216966
16	1	5.695329034391527
16	2	7.933184523809523
16	3	15.200363756613758
16	4	10.852984457671958
16	5	9.109656084656088
16	6	14.46715443121693
16	7	9.741426917989415
16	8	13.680778769841272
16	9	11.695982142857144
16	10	7.963219246031745
16	11	11.993320105820104
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114	6	-10.53284556878307
114	7	-10.591906415343919
114	8	-8.81922123015873
114	9	-11.970684523809522
114	10	-9.536780753968253
114	11	-8.84001322751323
114	12	-15.81773313492063
114	13	-14.626207010582016
114	14	-9.843882275132275
114	15	-15.580877976190479
114	16	-9.194510582010578
114	17	-11.493568121693118
114	18	-14.523950066137573
114	19	-7.085309193121695
114	20	-6.946569113756613
114	21	-10.397519841269837
114	22	-2.17461970899471
114	23	-9.650471230158729
114	24	-6.828538359788363
114	25	-4.2779100529100536
114	26	-0.3355654761904745
114	27	0.08480489417989645
114	28	0.8671461640211682
114	29	0.4573164682539641
114	30	1.4507357804232814
114	31	-8.365550595238096
114	32	-7.09834656084656
114	33	-7.3191633597883605
114	34	-7.211772486772487
114	35	-6.571271494708995
114	36	-6.568096891534392
114	37	-6.401173941798943
114	38	-6.368212632275132
114	39	-5.380439814814816
114	40	-5.942749669312169
115	1	5.861995701058195
115	2	10.433184523809523
115	3	-0.9663029100529101
115	4	9.18631779100529
115	5	9.94298941798942
115	6	9.96715443121693
115	7	4.741426917989415
115	8	8.847445436507936
115	9	2.6959821428571438
115	10	10.129885912698413
115	11	5.4933201058201035
115	12	-2.651066468253962
115	13	-3.45954034391535
115	14	2.989451058201059
115	15	-0.2475446428571466
115	16	6.472156084656088
115	17	-4.3269014550264515
115	18	1.642716600529095
115	19	6.914690806878305
115	20	-7.446569113756613
115	21	9.76914682539683
115	22	7.658713624338624
115	23	0.349528769841271
115	24	-7.328538359788363
115	25	-6.7779100529100536
115	26	3.4977678571428594
115	27	3.9181382275132304
115	28	2.5338128306878342
115	29	4.290649801587298
115	30	5.284069113756615
115	31	5.634449404761904
115	32	9.90165343915344
115	33	6.6808366402116395
115	34	-7.211772486772487
115	35	-6.571271494708995
115	36	-6.568096891534392
115	37	-6.401173941798943
115	38	-6.368212632275132
115	39	-5.380439814814816
115	40	-5.942749669312169
117	1	9.361995701058195
117	2	18.933184523809523
117	3	17.53369708994709
117	4	9.68631779100529
117	5	-7.05701058201058
117	6	4.46715443121693
117	7	11.741426917989415
117	8	4.847445436507936
117	9	9.695982142857144
117	10	7.129885912698413
117	11	8.493320105820104
117	12	7.848933531746038
117	13	-4.95954034391535
117	14	11.489451058201059
117	15	13.752455357142853
117	16	16.47215608465609
117	17	17.17309854497355
117	18	17.142716600529095
117	19	16.914690806878305
117	20	16.053430886243387
117	21	22.26914682539683
117	22	19.158713624338624
117	23	16.34952876984127
117	24	22.17146164021164
117	25	22.72208994708995
117	26	20.99776785714286
117	27	24.418138227513232
117	28	21.533812830687836
117	29	21.7906498015873
117	30	25.784069113756615
117	31	15.634449404761904
117	32	13.90165343915344
117	33	10.68083664021164
117	34	16.78822751322751
117	35	17.428728505291005
117	36	24.431903108465608
117	37	27.598826058201055
117	38	24.63178736772487
117	39	14.619560185185184
117	40	28.05725033068783
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
4	470.0
5	11.0
6	14.0
7	10.0
8	20.0
9	30.0
10	59.0
11	78.0
12	98.0
13	80.0
14	107.0
15	114.0
16	138.0
17	122.0
18	142.0
19	127.0
20	109.0
21	138.0
22	135.0
23	141.0
24	88.0
25	112.0
26	98.0
27	84.0
28	72.0
29	46.0
30	26.0
31	16.0
32	4.0
33	4.0
34	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	0.0	0.0	100.0
3	0.0	0.0	0.0	100.0
4	0.0	0.0	100.0	0.0
5	0.0	100.0	0.0	0.0
6	100.0	0.0	0.0	0.0
7	98.81173412551058	0.9654660230226514	0.18566654288897141	0.03713330857779428
8	97.1439169139466	0.8902077151335311	0.7047477744807121	1.261127596439169
9	94.69190794357833	3.229398663697105	1.2249443207126949	0.8537490720118782
10	33.51872450871339	39.22877271041899	12.829069336299593	14.423433444568037
11	23.46182357301705	32.65381764269829	20.644922164566346	23.239436619718308
12	34.43291326908821	17.420311341734617	24.277242401779095	23.869532987398074
13	27.98369162342476	20.904373610081542	25.129725722757602	25.9822090437361
14	24.53669384729429	26.98295033358043	26.20459599703484	22.27575982209044
15	24.166048925129726	26.64936990363232	25.685693106004447	23.498888065233505
16	24.166048925129726	22.60934025203855	31.430689399555227	21.7939214232765
17	26.167531504818385	27.65011119347665	22.461082283172722	23.721275018532246
18	30.985915492957744	25.277983691623422	22.831727205337288	20.904373610081542
19	21.309036816660466	18.148010412792857	18.33395314243213	42.20899962811454
20	26.278724981467754	27.538917716827278	18.754633061527056	27.42772424017791
21	24.796145292809488	21.90511489992587	26.834692364714602	26.464047442550036
22	22.68346923647146	20.34840622683469	21.312083024462563	35.65604151223128
23	24.24017790956264	23.647146034099332	27.27946627131208	24.833209785025943
24	20.949202817945867	25.139043381535036	23.136818687430477	30.77493511308862
25	22.469410456062292	25.658138672599186	24.768261030774934	27.104189840563592
26	20.350354081252327	25.046589638464408	29.14647782333209	25.456578456951174
27	20.83024462564863	21.46034099332839	30.355819125277982	27.353595255744995
28	15.869484612532444	23.507601038190582	32.22098628105302	28.401928068223953
29	17.378388414407723	20.534719643520237	33.97697734868177	28.109914593390272
30	15.484589676940216	20.608986260675827	38.878574080950614	25.02784998143335
31	14.34410999628391	24.00594574507618	39.35340022296544	22.296544035674472
32	17.93921423276501	18.013343217197924	39.99258710155671	24.054855448480357
33	14.084507042253522	20.49666419570052	42.95774647887324	22.461082283172722
34	12.23128243143069	20.16308376575241	46.627131208302444	20.978502594514456
35	13.676797627872498	20.16308376575241	45.5522609340252	20.607857672349887
36	11.78650852483321	19.792438843587842	44.25500370644922	24.166048925129726
37	10.081541882876206	19.38472942920682	46.81245366938473	23.721275018532246
38	11.638250555967383	18.977020014825797	47.85025945144552	21.534469977761304
39	14.899925871015569	20.200148257968866	45.25574499629355	19.644180874722018
40	10.415122312824314	25.500370644922164	46.10822831727205	17.97627872498147
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	4.5
17	9.0
18	9.0
19	11.5
20	14.0
21	16.5
22	19.0
23	19.0
24	25.0
25	31.0
26	33.0
27	35.0
28	35.0
29	41.5
30	48.0
31	57.5
32	67.0
33	67.0
34	75.0
35	83.0
36	87.5
37	92.0
38	92.0
39	115.5
40	139.0
41	161.5
42	184.0
43	184.0
44	185.5
45	187.0
46	200.0
47	213.0
48	213.0
49	201.5
50	190.0
51	183.5
52	177.0
53	177.0
54	167.5
55	158.0
56	155.5
57	153.0
58	153.0
59	154.0
60	155.0
61	149.0
62	143.0
63	143.0
64	145.5
65	148.0
66	144.0
67	140.0
68	140.0
69	124.0
70	108.0
71	98.5
72	89.0
73	89.0
74	75.0
75	61.0
76	45.5
77	30.0
78	30.0
79	23.5
80	17.0
81	10.5
82	4.0
83	4.0
84	3.5
85	3.0
86	2.0
87	1.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.18532246108228317
8	0.07412898443291327
9	0.14825796886582654
10	0.037064492216456635
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.3335804299481097
20	0.0
21	0.0
22	0.0
23	0.0
24	0.037064492216456635
25	0.037064492216456635
26	0.5559673832468496
27	0.0
28	0.037064492216456635
29	0.18532246108228317
30	0.18532246108228317
31	0.25945144551519644
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
40	2698.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	96.25648628613787
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.99653446284174	93.36545589325426
2	2.502887947631883	4.818383988139362
3	0.19252984212552945	0.5559673832468496
4	0.23103581055063535	0.8895478131949592
5	0.07701193685021178	0.37064492216456635
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5	0.18532246108228317	No Hit
GCCTAGGGGGCCGAATAGCCTCTCCACCCAAGCGGCCCGA	5	0.18532246108228317	No Hit
GCCTAGGGGCCATCTCATCCCTGCGTGTCTCCGACTCCGA	4	0.14825796886582654	No Hit
GCCTAGGGGTAGAGATGTGGAGGAATACCGCAGGGATGAG	4	0.14825796886582654	No Hit
GCCTAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG	4	0.14825796886582654	No Hit
GCCTAGGGGTCGAGAGTCGATTTTTTTTTTTTTTTTTTTT	4	0.14825796886582654	No Hit
GCCTAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT	4	0.14825796886582654	No Hit
GCCTAGGGGGTCTCTTTTCCTCTGGCGCGGTGAGTGAGTT	4	0.14825796886582654	No Hit
GCCTAGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT	3	0.1111934766493699	No Hit
GCCTAGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3	0.1111934766493699	No Hit
GCCTAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCC	3	0.1111934766493699	No Hit
GCCTAGGGGGATCCAGTGACAGATACCATTAGCTTCAAGA	3	0.1111934766493699	No Hit
GCCTAGGGGAGCAGTGTATCCGCAGAGTCGACTTTTCTTT	3	0.1111934766493699	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.037064492216456635	0.0	0.0	0.0	0.0
26	0.037064492216456635	0.0	0.0	0.0	0.0
27	0.037064492216456635	0.0	0.0	0.0	0.0
28	0.037064492216456635	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGGGCA	25	1.2963581E-4	34.92157	5
TAGGGGG	100	0.0	34.92157	4
AGGGGGT	20	0.00201797	34.921566	5
AGGGGGG	30	8.205247E-6	34.921566	5
AGGGGGC	20	0.00201797	34.921566	5
AGGGGGA	30	8.205247E-6	34.921566	5
TAGGGGT	20	0.00201797	34.921566	4
CTAGGGG	270	0.0	31.712965	3
TAGGGGA	85	0.0	30.813148	4
CCTAGGG	295	0.0	30.186441	2
GCCTAGG	300	0.0	29.68333	1
AGGGGAA	60	7.912604E-10	29.101307	5
TAGGGGC	60	7.912604E-10	29.101307	4
>>END_MODULE