FastQCFastQC Report
Fri 17 Jun 2016
SRR1525919_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525919_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3621
Sequences flagged as poor quality0
Sequence length40
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA802.2093344380005524No Hit
GCCTAGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT501.3808340237503451No Hit
GCCTAGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG320.8837337752002209No Hit
GCCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC260.7180336923501794No Hit
GCCTAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT200.5523336095001381No Hit
GCCTAGGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT160.44186688760011045No Hit
GCCTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA140.38663352665009665No Hit
GCCTAGGGGAAAAAAAAAAAACAAAAAAAAAAAAAAAAAA110.303783485225076No Hit
GCCTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110.303783485225076No Hit
GCCTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG90.24855012427506215No Hit
GCCTAGGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG90.24855012427506215No Hit
GCCTAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTT80.22093344380005522No Hit
GCCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCA60.16570008285004142No Hit
GCCTAGGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAA60.16570008285004142No Hit
GCCTAGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.16570008285004142No Hit
GCCTAGGGGACTAATCCTAGCCCTAGCCCTACACAAATAT50.13808340237503453No Hit
GCCTAGGGGAAAAAAAAACAAAAAAAAAAAAAAAAAAAAA50.13808340237503453No Hit
GCCTAGGGGGTGTTCTTTATTAATATCCTAACACTCCTCG50.13808340237503453No Hit
GCCTAGGGGGATCTGCTGAGGTCCTGGATGCCTCATCCCT40.11046672190002761No Hit
GCCTAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG40.11046672190002761No Hit
GCCTAGGGGAATTATCGCAATTCCTACCGGTGTCAAAGTA40.11046672190002761No Hit
GCCTAGGGGGTCACCTTAGCTGGGAAAATCATTGCAAGCA40.11046672190002761No Hit
GCCTAGGGGGCTGCCTAGGGGTCACAGATCCTCTTCTGAG40.11046672190002761No Hit
GCCTAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCG40.11046672190002761No Hit
GCCTAGGGGGAGTGCAGTGCTGCCTAGGGGAAAAAAAAAA40.11046672190002761No Hit
GCCTAGGGGAGCATCATGGTTCTGGCCTTTTCCACATTCA40.11046672190002761No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGAGT200.00232202934.32394428
AGATGAG200.00232202934.32394427
ATGAGTT200.00232202934.32394429
GAGATGA200.00232202934.32394426
TGAGATG200.00232202934.32394425
AGGGGGC357.716735E-733.847225
AGGGGGA357.716735E-733.847225
AGGGGAT301.1505699E-533.847225
GTTTTTG200.002491910733.8472233
GAGTTTT200.002491910733.8472231
GGGGGTG251.7002184E-433.847226
TAGGGGC405.124457E-833.847224
AGTTTTT200.002491910733.8472232
TGAGTTT200.002491910733.8472230
TAGGGGG1950.032.111474
GCCTAGG3800.032.065791
CCTAGGG3800.031.620432
CTAGGGG3700.031.1028543
AGGGGGT603.8198777E-1131.026625
TAGGGGA900.030.086424
Produced by FastQC (version 0.11.5)