##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1525919_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3621
Sequences flagged as poor quality	0
Sequence length	40
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	17.216790941728803	21.0	4.0	30.0	4.0	33.0
2	12.811654239160452	4.0	4.0	23.0	4.0	30.0
3	11.829328914664456	4.0	4.0	20.0	4.0	28.0
4	12.429991714995857	4.0	4.0	22.0	4.0	30.0
5	9.971554819110743	4.0	4.0	15.0	4.0	26.0
6	11.374482187241094	4.0	4.0	21.0	4.0	30.0
7	10.9939243302955	4.0	4.0	20.0	4.0	30.0
8	10.500690417011874	4.0	4.0	18.0	4.0	29.0
9	10.300745650372825	4.0	4.0	17.0	4.0	28.0
10	9.894504280585474	4.0	4.0	15.0	4.0	28.0
11	10.086716376691522	4.0	4.0	14.0	4.0	30.0
12	10.098591549295774	4.0	4.0	14.0	4.0	30.0
13	9.651201325600663	4.0	4.0	11.0	4.0	30.0
14	9.594034797017398	4.0	4.0	10.0	4.0	30.0
15	9.51808892571113	4.0	4.0	4.0	4.0	31.0
16	7.986467826567247	4.0	4.0	4.0	4.0	25.0
17	7.6219276442971555	4.0	4.0	4.0	4.0	22.0
18	7.56227561447114	4.0	4.0	4.0	4.0	22.0
19	7.40016570008285	4.0	4.0	4.0	4.0	22.0
20	7.325876829605082	4.0	4.0	4.0	4.0	21.0
21	7.11847555923778	4.0	4.0	4.0	4.0	20.0
22	7.135045567522784	4.0	4.0	4.0	4.0	20.0
23	7.0444628555647615	4.0	4.0	4.0	4.0	20.0
24	6.873791770229219	4.0	4.0	4.0	4.0	19.0
25	7.066556199944767	4.0	4.0	4.0	4.0	20.0
26	7.191107428887047	4.0	4.0	4.0	4.0	21.0
27	7.249102457884562	4.0	4.0	4.0	4.0	22.0
28	7.2935653134493235	4.0	4.0	4.0	4.0	22.0
29	6.966859983429992	4.0	4.0	4.0	4.0	19.0
30	7.0588235294117645	4.0	4.0	4.0	4.0	21.0
31	7.48163490748412	4.0	4.0	4.0	4.0	25.0
32	7.406517536592101	4.0	4.0	4.0	4.0	25.0
33	7.365921016293841	4.0	4.0	4.0	4.0	24.0
34	7.346589339961336	4.0	4.0	4.0	4.0	24.0
35	7.1850317591825466	4.0	4.0	4.0	4.0	23.0
36	7.224523612261806	4.0	4.0	4.0	4.0	24.0
37	7.018227009113504	4.0	4.0	4.0	4.0	20.0
38	6.898646782656725	4.0	4.0	4.0	4.0	19.0
39	6.934548467274234	4.0	4.0	4.0	4.0	20.0
40	6.979839823253245	4.0	4.0	4.0	4.0	21.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	-11.777736441798945
1	2	-8.150537367724866
1	3	-7.160069444444446
1	4	-7.9768601190476165
1	5	-5.983775628306882
1	6	-7.170291832010582
1	7	-6.721970072751324
1	8	-6.318948412698413
1	9	-6.163748346560848
1	10	-5.543497850529102
1	11	-6.47001488095238
1	12	-5.8945023148148135
1	13	-5.959738756613756
1	14	-6.051793981481481
1	15	-5.703364748677249
1	16	-4.5911292989418
1	17	-3.8922577711640214
1	18	-3.6422040343915327
1	19	-4.148544973544974
1	20	-4.140021494708995
1	21	-4.047776124338625
1	22	-3.6684317129629633
1	23	-3.334255125661376
1	24	-3.149388227513228
1	25	-2.930613425925924
1	26	-3.256307870370371
1	27	-3.630427414021164
1	28	-3.3310598544973544
1	29	-3.41935763888889
1	30	-3.2352141203703697
1	31	-3.0691096230158736
1	32	-3.1939732142857133
1	33	-2.9323123346560847
1	34	-2.806638558201059
1	35	-2.997131283068782
1	36	-2.895568783068782
1	37	-2.9639880952380944
1	38	-2.8982514880952372
1	39	-3.130708498677248
1	40	-2.9165302579365076
2	1	6.555596891534387
2	2	9.849462632275134
2	3	5.173263888888886
2	4	2.6898065476190496
2	5	-1.9837756283068817
2	6	4.829708167989418
2	7	6.611363260582008
2	8	5.014384920634921
2	9	-3.1637483465608476
2	10	3.4565021494708983
2	11	3.1966517857142858
2	12	3.7721643518518526
2	13	6.373594576719576
2	14	8.948206018518519
2	15	8.296635251322751
2	16	7.742204034391532
2	17	-0.8922577711640214
2	18	8.6911292989418
2	19	11.184788359788358
2	20	6.5266451719576715
2	21	7.952223875661375
2	22	2.9982349537037027
2	23	12.332411541005293
2	24	-0.4827215608465609
2	25	10.402719907407409
2	26	6.410358796296295
2	27	7.70290591931217
2	28	10.335606812169313
2	29	8.247309027777776
2	30	11.098119212962963
2	31	15.26422371031746
2	32	8.806026785714288
2	33	11.067687665343914
2	34	12.86002810846561
2	35	7.669535383597884
2	36	14.771097883597886
2	37	8.369345238095239
2	38	11.101748511904763
2	39	9.202624834656085
2	40	15.75013640873016
3	1	-11.777736441798945
3	2	-8.150537367724866
3	3	-7.160069444444446
3	4	-7.9768601190476165
3	5	-5.983775628306882
3	6	-7.170291832010582
3	7	-6.721970072751324
3	8	-6.318948412698413
3	9	-6.163748346560848
3	10	-5.543497850529102
3	11	-6.47001488095238
3	12	-5.8945023148148135
3	13	-5.959738756613756
3	14	-6.051793981481481
3	15	-5.703364748677249
3	16	-4.5911292989418
3	17	-3.8922577711640214
3	18	-3.6422040343915327
3	19	-4.148544973544974
3	20	-4.140021494708995
3	21	-4.047776124338625
3	22	-3.6684317129629633
3	23	-3.334255125661376
3	24	-3.149388227513228
3	25	-2.930613425925924
3	26	-3.256307870370371
3	27	-3.630427414021164
3	28	-3.3310598544973544
3	29	-3.41935763888889
3	30	-3.2352141203703697
3	31	-3.0691096230158736
3	32	-3.1939732142857133
3	33	-2.9323123346560847
3	34	-2.806638558201059
3	35	-2.997131283068782
3	36	-2.895568783068782
3	37	-2.9639880952380944
3	38	-2.8982514880952372
3	39	-3.130708498677248
3	40	-2.9165302579365076
4	1	-3.6348792989418026
4	2	-5.436251653439151
4	3	-3.5886408730158745
4	4	-3.9768601190476165
4	5	-4.983775628306882
4	6	-3.0274346891534396
4	7	-5.293398644179895
4	8	-3.0332341269841274
4	9	-2.449462632275133
4	10	-2.114926421957673
4	11	-3.1843005952380947
4	12	-2.180216600529099
4	13	-2.6740244708994707
4	14	-1.9089368386243386
4	15	-5.703364748677249
4	16	-4.5911292989418
4	17	-3.8922577711640214
4	18	-3.6422040343915327
4	19	-4.148544973544974
4	20	-4.140021494708995
4	21	-4.047776124338625
4	22	-3.6684317129629633
4	23	-3.334255125661376
4	24	-3.149388227513228
4	25	-2.930613425925924
4	26	-3.256307870370371
4	27	-3.630427414021164
4	28	-3.3310598544973544
4	29	-3.41935763888889
4	30	-3.2352141203703697
4	31	-3.0691096230158736
4	32	-3.1939732142857133
4	33	-2.9323123346560847
4	34	-2.806638558201059
4	35	-2.997131283068782
4	36	-2.895568783068782
4	37	-2.9639880952380944
4	38	-2.8982514880952372
4	39	-3.130708498677248
4	40	-2.9165302579365076
5	1	-11.777736441798945
5	2	-8.150537367724866
5	3	-7.160069444444446
5	4	-7.9768601190476165
5	5	-5.983775628306882
5	6	-7.170291832010582
5	7	-6.721970072751324
5	8	-6.318948412698413
5	9	-6.163748346560848
5	10	-5.543497850529102
5	11	-6.47001488095238
5	12	-5.8945023148148135
5	13	-5.959738756613756
5	14	-6.051793981481481
5	15	-5.703364748677249
5	16	-4.5911292989418
5	17	-3.8922577711640214
5	18	-3.6422040343915327
5	19	-4.148544973544974
5	20	-4.140021494708995
5	21	-4.047776124338625
5	22	-3.6684317129629633
5	23	-3.334255125661376
5	24	-3.149388227513228
5	25	-2.930613425925924
5	26	-3.256307870370371
5	27	-3.630427414021164
5	28	-3.3310598544973544
5	29	-3.41935763888889
5	30	-3.2352141203703697
5	31	-3.0691096230158736
5	32	-3.1939732142857133
5	33	-2.9323123346560847
5	34	-2.806638558201059
5	35	-2.997131283068782
5	36	-2.895568783068782
5	37	-2.9639880952380944
5	38	-2.8982514880952372
5	39	-3.130708498677248
5	40	-2.9165302579365076
6	1	17.555596891534393
6	2	15.182795965608467
6	3	7.173263888888886
6	4	9.356473214285716
6	5	15.34955770502645
6	6	5.496374834656086
6	7	11.278029927248676
6	8	20.347718253968253
6	9	21.836251653439152
6	10	7.78983548280423
6	11	13.196651785714288
6	12	12.772164351851854
6	13	20.040261243386244
6	14	17.94820601851852
6	15	14.629968584656083
6	16	21.07553736772487
6	17	24.441075562169313
6	18	20.024462632275135
6	19	22.184788359788357
6	20	21.19331183862434
6	21	17.952223875661375
6	22	4.664901620370371
6	23	16.332411541005293
6	24	16.183945105820104
6	25	-0.930613425925924
6	26	5.410358796296295
6	27	5.036239252645502
6	28	4.0022734788359795
6	29	3.913975694444444
6	30	-0.5685474537037027
6	31	4.26422371031746
6	32	6.806026785714287
6	33	3.0676876653439153
6	34	2.193361441798941
6	35	6.336202050264552
6	36	3.104431216931218
6	37	2.0360119047619056
6	38	6.768415178571429
6	39	-3.130708498677248
6	40	-2.9165302579365076
7	1	9.472263558201055
7	2	11.599462632275134
7	3	8.589930555555554
7	4	8.273139880952384
7	5	10.516224371693118
7	6	-3.420291832010582
7	7	5.278029927248676
7	8	12.431051587301587
7	9	9.336251653439152
7	10	12.956502149470898
7	11	1.2799851190476197
7	12	9.105497685185187
7	13	5.540261243386244
7	14	1.198206018518519
7	15	6.046635251322751
7	16	12.4088707010582
7	17	9.607742228835978
7	18	9.357795965608467
7	19	7.851455026455026
7	20	5.359978505291005
7	21	14.452223875661375
7	22	11.081568287037037
7	23	9.165744874338625
7	24	7.100611772486772
7	25	12.319386574074077
7	26	8.743692129629629
7	27	9.869572585978837
7	28	11.418940145502646
7	29	10.08064236111111
7	30	9.514785879629631
7	31	14.680890376984127
7	32	11.806026785714288
7	33	13.567687665343914
7	34	11.193361441798942
7	35	16.252868716931218
7	36	19.354431216931218
7	37	19.036011904761907
7	38	16.851748511904763
7	39	18.119291501322753
7	40	15.083469742063492
8	1	6.055596891534387
8	2	-0.6505373677248656
8	3	5.339930555555554
8	4	-1.4768601190476165
8	5	1.5162243716931183
8	6	5.16304150132275
8	7	4.278029927248676
8	8	0.681051587301587
8	9	0.8362516534391524
8	10	2.9565021494708983
8	11	7.52998511904762
8	12	8.605497685185187
8	13	8.040261243386244
8	14	9.114872685185187
8	15	-5.703364748677249
8	16	-4.5911292989418
8	17	-3.8922577711640214
8	18	-3.6422040343915327
8	19	-4.148544973544974
8	20	-4.140021494708995
8	21	-4.047776124338625
8	22	-3.6684317129629633
8	23	-3.334255125661376
8	24	-3.149388227513228
8	25	-2.930613425925924
8	26	-3.256307870370371
8	27	-3.630427414021164
8	28	-3.3310598544973544
8	29	-3.41935763888889
8	30	-3.2352141203703697
8	31	-3.0691096230158736
8	32	-3.1939732142857133
8	33	-2.9323123346560847
8	34	-2.806638558201059
8	35	-2.997131283068782
8	36	-2.895568783068782
8	37	-2.9639880952380944
8	38	-2.8982514880952372
8	39	-3.130708498677248
8	40	-2.9165302579365076
9	1	1.8889302248677229
9	2	-4.150537367724866
9	3	-3.1600694444444457
9	4	-5.4768601190476165
9	5	-3.4837756283068817
9	6	-2.503625165343916
9	7	-6.721970072751324
9	8	-6.318948412698413
9	9	-6.163748346560848
9	10	-5.543497850529102
9	11	-6.47001488095238
9	12	-5.8945023148148135
9	13	-5.959738756613756
9	14	-6.051793981481481
9	15	-5.703364748677249
9	16	-4.5911292989418
9	17	-3.8922577711640214
9	18	-3.6422040343915327
9	19	-4.148544973544974
9	20	-4.140021494708995
9	21	-4.047776124338625
9	22	-3.6684317129629633
9	23	-3.334255125661376
9	24	-3.149388227513228
9	25	-2.930613425925924
9	26	-3.256307870370371
9	27	-3.630427414021164
9	28	-3.3310598544973544
9	29	-3.41935763888889
9	30	-3.2352141203703697
9	31	-3.0691096230158736
9	32	-3.1939732142857133
9	33	-2.9323123346560847
9	34	-2.806638558201059
9	35	-2.997131283068782
9	36	-2.895568783068782
9	37	-2.9639880952380944
9	38	-2.8982514880952372
9	39	-3.130708498677248
9	40	-2.9165302579365076
11	1	-3.111069775132279
11	2	-0.8172040343915317
11	3	0.17326388888888822
11	4	0.35647321428571743
11	5	-4.983775628306882
11	6	2.496374834656084
11	7	-6.721970072751324
11	8	-6.318948412698413
11	9	-6.163748346560848
11	10	-5.543497850529102
11	11	-6.47001488095238
11	12	-5.8945023148148135
11	13	-5.959738756613756
11	14	-6.051793981481481
11	15	-5.703364748677249
11	16	-4.5911292989418
11	17	-3.8922577711640214
11	18	-3.6422040343915327
11	19	-4.148544973544974
11	20	-4.140021494708995
11	21	-4.047776124338625
11	22	-3.6684317129629633
11	23	-3.334255125661376
11	24	-3.149388227513228
11	25	-2.930613425925924
11	26	-3.256307870370371
11	27	-3.630427414021164
11	28	-3.3310598544973544
11	29	-3.41935763888889
11	30	-3.2352141203703697
11	31	-3.0691096230158736
11	32	-3.1939732142857133
11	33	-2.9323123346560847
11	34	-2.806638558201059
11	35	-2.997131283068782
11	36	-2.895568783068782
11	37	-2.9639880952380944
11	38	-2.8982514880952372
11	39	-3.130708498677248
11	40	-2.9165302579365076
12	1	10.422263558201054
12	2	7.849462632275134
12	3	7.439930555555556
12	4	0.6231398809523832
12	5	10.816224371693119
12	6	1.4297081679894177
12	7	7.878029927248678
12	8	7.481051587301588
12	9	5.236251653439153
12	10	4.456502149470898
12	11	3.3299851190476204
12	12	3.305497685185186
12	13	9.440261243386242
12	14	8.348206018518518
12	15	10.69663525132275
12	16	9.208870701058201
12	17	4.107742228835979
12	18	10.557795965608467
12	19	6.251455026455027
12	20	3.659978505291006
12	21	7.952223875661375
12	22	10.931568287037038
12	23	7.065744874338624
12	24	9.650611772486773
12	25	10.069386574074077
12	26	10.34369212962963
12	27	7.769572585978836
12	28	12.668940145502646
12	29	13.180642361111111
12	30	12.764785879629631
12	31	11.530890376984129
12	32	14.406026785714289
12	33	9.867687665343915
12	34	11.993361441798942
12	35	12.802868716931219
12	36	10.904431216931219
12	37	12.236011904761906
12	38	6.9017485119047635
12	39	9.469291501322754
12	40	11.483469742063491
13	1	2.5079778439153397
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114	2	-8.150537367724866
114	3	-7.160069444444446
114	4	-7.9768601190476165
114	5	-5.983775628306882
114	6	-7.170291832010582
114	7	-6.721970072751324
114	8	-6.318948412698413
114	9	-6.163748346560848
114	10	-5.543497850529102
114	11	-6.47001488095238
114	12	-5.8945023148148135
114	13	-5.959738756613756
114	14	-6.051793981481481
114	15	-5.703364748677249
114	16	-4.5911292989418
114	17	-3.8922577711640214
114	18	-3.6422040343915327
114	19	-4.148544973544974
114	20	-4.140021494708995
114	21	-4.047776124338625
114	22	-3.6684317129629633
114	23	-3.334255125661376
114	24	-3.149388227513228
114	25	-2.930613425925924
114	26	-3.256307870370371
114	27	-3.630427414021164
114	28	-3.3310598544973544
114	29	-3.41935763888889
114	30	-3.2352141203703697
114	31	-3.0691096230158736
114	32	-3.1939732142857133
114	33	-2.9323123346560847
114	34	-2.806638558201059
114	35	-2.997131283068782
114	36	-2.895568783068782
114	37	-2.9639880952380944
114	38	-2.8982514880952372
114	39	-3.130708498677248
114	40	-2.9165302579365076
115	1	-11.777736441798945
115	2	-8.150537367724866
115	3	-7.160069444444446
115	4	-7.9768601190476165
115	5	-5.983775628306882
115	6	-7.170291832010582
115	7	-6.721970072751324
115	8	-6.318948412698413
115	9	-6.163748346560848
115	10	-5.543497850529102
115	11	-6.47001488095238
115	12	-5.8945023148148135
115	13	-5.959738756613756
115	14	-6.051793981481481
115	15	-5.703364748677249
115	16	-4.5911292989418
115	17	-3.8922577711640214
115	18	-3.6422040343915327
115	19	-4.148544973544974
115	20	-4.140021494708995
115	21	-4.047776124338625
115	22	-3.6684317129629633
115	23	-3.334255125661376
115	24	-3.149388227513228
115	25	-2.930613425925924
115	26	-3.256307870370371
115	27	-3.630427414021164
115	28	-3.3310598544973544
115	29	-3.41935763888889
115	30	-3.2352141203703697
115	31	-3.0691096230158736
115	32	-3.1939732142857133
115	33	-2.9323123346560847
115	34	-2.806638558201059
115	35	-2.997131283068782
115	36	-2.895568783068782
115	37	-2.9639880952380944
115	38	-2.8982514880952372
115	39	-3.130708498677248
115	40	-2.9165302579365076
116	1	-5.277736441798945
116	2	-4.150537367724866
116	3	-4.160069444444446
116	4	-2.2268601190476165
116	5	-4.983775628306882
116	6	-0.670291832010582
116	7	-6.721970072751324
116	8	-6.318948412698413
116	9	-6.163748346560848
116	10	-5.543497850529102
116	11	-6.47001488095238
116	12	-5.8945023148148135
116	13	-5.959738756613756
116	14	-6.051793981481481
116	15	-5.703364748677249
116	16	-4.5911292989418
116	17	-3.8922577711640214
116	18	-3.6422040343915327
116	19	-4.148544973544974
116	20	-4.140021494708995
116	21	-4.047776124338625
116	22	-3.6684317129629633
116	23	-3.334255125661376
116	24	-3.149388227513228
116	25	-2.930613425925924
116	26	-3.256307870370371
116	27	-3.630427414021164
116	28	-3.3310598544973544
116	29	-3.41935763888889
116	30	-3.2352141203703697
116	31	-3.0691096230158736
116	32	-3.1939732142857133
116	33	-2.9323123346560847
116	34	-2.806638558201059
116	35	-2.997131283068782
116	36	-2.895568783068782
116	37	-2.9639880952380944
116	38	-2.8982514880952372
116	39	-3.130708498677248
116	40	-2.9165302579365076
117	1	-11.777736441798945
117	2	-8.150537367724866
117	3	-7.160069444444446
117	4	-7.9768601190476165
117	5	-5.983775628306882
117	6	-7.170291832010582
117	7	-6.721970072751324
117	8	-6.318948412698413
117	9	-6.163748346560848
117	10	-5.543497850529102
117	11	-6.47001488095238
117	12	-5.8945023148148135
117	13	-5.959738756613756
117	14	-6.051793981481481
117	15	-5.703364748677249
117	16	-4.5911292989418
117	17	-3.8922577711640214
117	18	-3.6422040343915327
117	19	-4.148544973544974
117	20	-4.140021494708995
117	21	-4.047776124338625
117	22	-3.6684317129629633
117	23	-3.334255125661376
117	24	-3.149388227513228
117	25	-2.930613425925924
117	26	-3.256307870370371
117	27	-3.630427414021164
117	28	-3.3310598544973544
117	29	-3.41935763888889
117	30	-3.2352141203703697
117	31	-3.0691096230158736
117	32	-3.1939732142857133
117	33	-2.9323123346560847
117	34	-2.806638558201059
117	35	-2.997131283068782
117	36	-2.895568783068782
117	37	-2.9639880952380944
117	38	-2.8982514880952372
117	39	-3.130708498677248
117	40	-2.9165302579365076
120	1	-11.777736441798945
120	2	-8.150537367724866
120	3	-7.160069444444446
120	4	-7.9768601190476165
120	5	-5.983775628306882
120	6	-7.170291832010582
120	7	-6.721970072751324
120	8	-6.318948412698413
120	9	-6.163748346560848
120	10	-5.543497850529102
120	11	-6.47001488095238
120	12	-5.8945023148148135
120	13	-5.959738756613756
120	14	-6.051793981481481
120	15	-5.703364748677249
120	16	-4.5911292989418
120	17	-3.8922577711640214
120	18	-3.6422040343915327
120	19	-4.148544973544974
120	20	-4.140021494708995
120	21	-4.047776124338625
120	22	-3.6684317129629633
120	23	-3.334255125661376
120	24	-3.149388227513228
120	25	-2.930613425925924
120	26	-3.256307870370371
120	27	-3.630427414021164
120	28	-3.3310598544973544
120	29	-3.41935763888889
120	30	-3.2352141203703697
120	31	-3.0691096230158736
120	32	-3.1939732142857133
120	33	-2.9323123346560847
120	34	-2.806638558201059
120	35	-2.997131283068782
120	36	-2.895568783068782
120	37	-2.9639880952380944
120	38	-2.8982514880952372
120	39	-3.130708498677248
120	40	-2.9165302579365076
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
4	2017.0
5	86.0
6	126.0
7	165.0
8	91.0
9	113.0
10	124.0
11	106.0
12	63.0
13	38.0
14	50.0
15	24.0
16	33.0
17	19.0
18	25.0
19	45.0
20	55.0
21	76.0
22	90.0
23	89.0
24	67.0
25	49.0
26	25.0
27	25.0
28	8.0
29	8.0
30	2.0
31	1.0
32	0.0
33	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	0.0	0.0	100.0
3	0.0	0.0	0.0	100.0
4	0.0	0.0	100.0	0.0
5	0.0	100.0	0.0	0.0
6	100.0	0.0	0.0	0.0
7	99.53025697706549	0.303951367781155	0.055263885051119094	0.11052777010223819
8	98.61802100608071	0.7462686567164178	0.3869541182974019	0.24875621890547264
9	94.60730088495575	2.5165929203539825	1.4380530973451326	1.4380530973451326
10	45.042805854736265	28.058547362607015	15.161557580778789	11.737089201877934
11	26.95388014360674	30.59928196630765	21.04391052195526	21.40292736813035
12	26.87103010218172	29.82601491300746	24.054128693731013	19.248826291079812
13	22.130013831258644	28.46473029045643	25.228215767634854	24.17704011065007
14	18.3927091963546	36.61971830985916	25.43496271748136	19.552609776304887
15	26.732946699806682	30.019331676332506	25.849212924606462	17.39850869925435
16	23.66749516708092	34.686550676608675	26.622479977906654	15.023474178403756
17	23.750345208505937	31.56586578293289	29.63269814968241	15.051090858878762
18	25.020712510356258	30.84783209058271	24.27506213753107	19.856393261529963
19	23.6318407960199	31.75787728026534	24.29519071310116	20.3150912106136
20	24.44076222038111	29.08036454018227	30.212648439657553	16.266224799779067
21	21.071527202430268	30.70974868820768	25.849212924606462	22.36951118475559
22	19.663076498204916	26.318696492681582	32.532449599558134	21.48577740955537
23	19.76229961304588	32.117191818684354	30.210060807075735	17.91044776119403
24	18.972659486329743	33.49903341618338	25.1864125932063	22.341894504280585
25	19.894998618402873	33.68333793865709	27.079303675048354	19.342359767891683
26	25.36031042128603	33.23170731707317	22.9490022172949	18.4589800443459
27	20.338043779440287	36.18730950401773	22.637849819894708	20.836796896647268
28	23.397790055248617	37.348066298342545	21.353591160220994	17.900552486187845
29	25.929007210205214	34.27620632279534	23.183582917359953	16.611203549639487
30	21.955260977630488	32.78099972383319	27.395747031206845	17.867992267329466
31	23.750345208505937	35.376967688483845	24.35791217895609	16.51477492405413
32	25.159147522834207	35.87046775532799	23.692222529753668	15.27816219208414
33	24.27506213753107	34.4103838718586	23.63987848660591	17.67467550400442
34	19.027892847279755	32.8086164043082	31.56586578293289	16.59762496547915
35	18.779342723004692	34.078983706158525	32.14581607290803	14.99585749792875
36	19.51219512195122	34.229490022172946	27.217294900221727	19.0410199556541
37	22.03811101905551	33.222866611433304	27.506213753106877	17.23280861640431
38	22.341894504280585	33.96851698425849	27.42336371168186	16.266224799779067
39	21.92764429715548	35.01795084230876	23.584645125655896	19.46975973487987
40	22.618061309030654	33.69235017950842	26.898646782656726	16.7909417288042
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.5
12	1.0
13	1.0
14	14.0
15	27.0
16	73.5
17	120.0
18	120.0
19	99.0
20	78.0
21	61.5
22	45.0
23	45.0
24	46.0
25	47.0
26	37.5
27	28.0
28	28.0
29	32.5
30	37.0
31	43.0
32	49.0
33	49.0
34	62.0
35	75.0
36	87.5
37	100.0
38	100.0
39	123.5
40	147.0
41	161.5
42	176.0
43	176.0
44	210.0
45	244.0
46	273.0
47	302.0
48	302.0
49	288.5
50	275.0
51	295.5
52	316.0
53	316.0
54	320.0
55	324.0
56	330.0
57	336.0
58	336.0
59	295.5
60	255.0
61	257.0
62	259.0
63	259.0
64	227.5
65	196.0
66	144.5
67	93.0
68	93.0
69	72.0
70	51.0
71	38.0
72	25.0
73	25.0
74	17.0
75	9.0
76	6.0
77	3.0
78	3.0
79	2.5
80	2.0
81	1.5
82	1.0
83	1.0
84	0.5
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.055233360950013806
8	0.08285004142502071
9	0.13808340237503453
10	0.0
11	0.0
12	0.0
13	0.16570008285004142
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.08285004142502071
20	0.0
21	0.0
22	0.0
23	0.08285004142502071
24	0.0
25	0.055233360950013806
26	0.3590168461750897
27	0.33140016570008285
28	0.027616680475006903
29	0.4142502071251036
30	0.0
31	0.0
32	0.22093344380005522
33	0.0
34	0.0
35	0.0
36	0.3590168461750897
37	0.0
38	0.0
39	0.0
40	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
40	3621.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.66694283347141
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.77820760799484	80.33692350179508
2	4.513217279174726	7.732670533001933
3	0.8704061895551257	2.236951118475559
4	0.2578981302385558	0.8837337752002209
5	0.09671179883945842	0.4142502071251036
6	0.09671179883945842	0.4971002485501243
7	0.0	0.0
8	0.032237266279819474	0.22093344380005522
9	0.06447453255963895	0.4971002485501243
>10	0.2578981302385558	4.971002485501243
>50	0.032237266279819474	2.2093344380005524
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GCCTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	80	2.2093344380005524	No Hit
GCCTAGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT	50	1.3808340237503451	No Hit
GCCTAGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG	32	0.8837337752002209	No Hit
GCCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC	26	0.7180336923501794	No Hit
GCCTAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT	20	0.5523336095001381	No Hit
GCCTAGGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT	16	0.44186688760011045	No Hit
GCCTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	14	0.38663352665009665	No Hit
GCCTAGGGGAAAAAAAAAAAACAAAAAAAAAAAAAAAAAA	11	0.303783485225076	No Hit
GCCTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	11	0.303783485225076	No Hit
GCCTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG	9	0.24855012427506215	No Hit
GCCTAGGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG	9	0.24855012427506215	No Hit
GCCTAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTT	8	0.22093344380005522	No Hit
GCCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCA	6	0.16570008285004142	No Hit
GCCTAGGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAA	6	0.16570008285004142	No Hit
GCCTAGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6	0.16570008285004142	No Hit
GCCTAGGGGACTAATCCTAGCCCTAGCCCTACACAAATAT	5	0.13808340237503453	No Hit
GCCTAGGGGAAAAAAAAACAAAAAAAAAAAAAAAAAAAAA	5	0.13808340237503453	No Hit
GCCTAGGGGGTGTTCTTTATTAATATCCTAACACTCCTCG	5	0.13808340237503453	No Hit
GCCTAGGGGGATCTGCTGAGGTCCTGGATGCCTCATCCCT	4	0.11046672190002761	No Hit
GCCTAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG	4	0.11046672190002761	No Hit
GCCTAGGGGAATTATCGCAATTCCTACCGGTGTCAAAGTA	4	0.11046672190002761	No Hit
GCCTAGGGGGTCACCTTAGCTGGGAAAATCATTGCAAGCA	4	0.11046672190002761	No Hit
GCCTAGGGGGCTGCCTAGGGGTCACAGATCCTCTTCTGAG	4	0.11046672190002761	No Hit
GCCTAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCG	4	0.11046672190002761	No Hit
GCCTAGGGGGAGTGCAGTGCTGCCTAGGGGAAAAAAAAAA	4	0.11046672190002761	No Hit
GCCTAGGGGAGCATCATGGTTCTGGCCTTTTCCACATTCA	4	0.11046672190002761	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.027616680475006903	0.0	0.0	0.0	0.0
6	0.027616680475006903	0.0	0.0	0.0	0.0
7	0.027616680475006903	0.0	0.0	0.0	0.0
8	0.027616680475006903	0.0	0.0	0.0	0.0
9	0.027616680475006903	0.0	0.0	0.0	0.0
10	0.027616680475006903	0.0	0.0	0.0	0.0
11	0.027616680475006903	0.0	0.0	0.0	0.0
12	0.027616680475006903	0.0	0.0	0.0	0.0
13	0.027616680475006903	0.0	0.0	0.0	0.0
14	0.055233360950013806	0.0	0.0	0.0	0.0
15	0.055233360950013806	0.0	0.0	0.0	0.0
16	0.055233360950013806	0.0	0.0	0.0	0.0
17	0.055233360950013806	0.0	0.0	0.0	0.0
18	0.055233360950013806	0.0	0.0	0.0	0.0
19	0.055233360950013806	0.0	0.0	0.0	0.0
20	0.055233360950013806	0.0	0.0	0.0	0.0
21	0.055233360950013806	0.0	0.0	0.0	0.0
22	0.055233360950013806	0.0	0.0	0.0	0.0
23	0.055233360950013806	0.0	0.0	0.0	0.0
24	0.055233360950013806	0.0	0.0	0.0	0.0
25	0.055233360950013806	0.0	0.0	0.0	0.0
26	0.055233360950013806	0.0	0.0	0.0	0.0
27	0.055233360950013806	0.0	0.0	0.0	0.0
28	0.055233360950013806	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATGAGT	20	0.002322029	34.323944	28
AGATGAG	20	0.002322029	34.323944	27
ATGAGTT	20	0.002322029	34.323944	29
GAGATGA	20	0.002322029	34.323944	26
TGAGATG	20	0.002322029	34.323944	25
AGGGGGC	35	7.716735E-7	33.84722	5
AGGGGGA	35	7.716735E-7	33.84722	5
AGGGGAT	30	1.1505699E-5	33.84722	5
GTTTTTG	20	0.0024919107	33.84722	33
GAGTTTT	20	0.0024919107	33.84722	31
GGGGGTG	25	1.7002184E-4	33.84722	6
TAGGGGC	40	5.124457E-8	33.84722	4
AGTTTTT	20	0.0024919107	33.84722	32
TGAGTTT	20	0.0024919107	33.84722	30
TAGGGGG	195	0.0	32.11147	4
GCCTAGG	380	0.0	32.06579	1
CCTAGGG	380	0.0	31.62043	2
CTAGGGG	370	0.0	31.102854	3
AGGGGGT	60	3.8198777E-11	31.02662	5
TAGGGGA	90	0.0	30.08642	4
>>END_MODULE