SRR1525918_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1525918_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13327
Sequences flagged as poor quality	0
Sequence length	56
%GC	43

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TTCATAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT	847	6.3555188714639455	No Hit
TTCATAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC	697	5.229984242515195	No Hit
TTCATAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC	234	1.755834021160051	No Hit
TTCATAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT	194	1.4556914534403842	No Hit
TTCATAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA	132	0.9904704734749005	No Hit
TTCATAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCCGATTGAACAAAAACTC	78	0.5852780070533503	No Hit
TTCATAGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCTC	73	0.547760186088392	No Hit
TTCATAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGCTCAATCCGCCCAATCT	66	0.49523523673745024	No Hit
TTCATAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGACCAAAAACTC	57	0.42770315900052525	No Hit
TTCATAGGGTCACAGATCCTCTGAAGGAGATTGGGCGGATTGAACAAAAACTCATC	53	0.39768890222855857	No Hit
TTCATAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	39	0.29263900352667516	No Hit
TTCATAGGGGTGCAGTGCTTTCATAGGGTCACAGATCCTCTTCTGAGATGAGTTTT	38	0.28513543933368346	No Hit
TTCATAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAAAAA	37	0.2776318751406918	No Hit
TTCATAGGGTCACAGATCCTCTCCTGAAGGAGATTGGGCGGATTGAACAAAAACTC	36	0.2701283109477002	No Hit
TTCATAGGGGAGTGCAGTGCTTTCATAGGGTCACAGATCCTCTTCTGAGATGAGTT	34	0.25512118256171684	No Hit
TTCATAGGGTCCCCCCTCCTCTTCTGAGATGAGTTTTTGTTCCCTCCGCCCAATCT	33	0.24761761836872512	No Hit
TTCATAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAGAT	30	0.2251069257897501	No Hit
TTCATAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA	25	0.18758910482479177	No Hit
TTCATAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTA	25	0.18758910482479177	No Hit
TTCATAGGGAGTGCAGTGCTTTCATAGGGTCACAGATCCTCTTCTGAAGGAGATTG	21	0.1575748480528251	No Hit
TTCATAGGGCAGAGTGCAGTGCTTTCATAGGGTCACAGATCCTCTTCTGAGATGAG	21	0.1575748480528251	No Hit
TTCATAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGATCAATCCGCCCAATCT	20	0.15007128385983343	No Hit
TTCATAGGGGAGTGCAGTGCTTTCATAGGGTCACAGATCCTCTTCTGAAGGAGATT	20	0.15007128385983343	No Hit
TTCATAGGGGTGCAGTGCTTTCATAGGGTCACAGATCCTCTTCTGAAGGAGATTGG	19	0.14256771966684173	No Hit
TTCATAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAAACT	19	0.14256771966684173	No Hit
TTCATAGGGCAGAGTGCAGTGCTTTCATAGGGTCACAGATCCTCTTCTGAAGGAGA	19	0.14256771966684173	No Hit
TTCATAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCTAATCCGCCCAATCT	19	0.14256771966684173	No Hit
TTCATAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGCTGTAGCGCCGGAAGA	19	0.14256771966684173	No Hit
TTCATAGGGAGTGCAGTGCTTTCATAGGGTCACAGATCCTCTTCTGAGATGAGTTT	18	0.1350641554738501	No Hit
TTCATAGGGTCACAGATCCTCTCCTGAAGGAGATTGGGCGCATTGAACAAAAACTC	17	0.12756059128085842	No Hit
TTCATAGGGTCATAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT	17	0.12756059128085842	No Hit
TTCATAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGCTCCATCCGCCCAATCT	16	0.12005702708786674	No Hit
TTCATAGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	15	0.11255346289487506	No Hit
TTCATAGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATG	15	0.11255346289487506	No Hit
TTCATAGGGTCCCCGCTCCTCTTCTGAAGGAGATTGGGCGGACTGCCCAAAAACTC	15	0.11255346289487506	No Hit
TTCATAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAAGAT	15	0.11255346289487506	No Hit
TTCATAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTTGTTCAATCCGCCCAATC	15	0.11255346289487506	No Hit
TTCATAGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTCA	15	0.11255346289487506	No Hit
TTCATAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTTAATCCGCCCAATCT	15	0.11255346289487506	No Hit
TTCATAGGGTCACATATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT	15	0.11255346289487506	No Hit
TTCATAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	14	0.1050498987018834	No Hit
TTCATAGGGAAGCAGTGGTATCAACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	14	0.1050498987018834	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTCCAA	35	4.2149622E-8	50.788464	38
GCATTGA	25	1.8001405E-5	50.788464	40
TTGTAGC	20	3.7323235E-4	50.78846	41
TCGAACA	15	0.0077513433	50.78846	40
GATCGAA	15	0.0077513433	50.78846	38
CCGTTGT	20	3.7323235E-4	50.78846	38
TTCCATC	15	0.0077513433	50.78846	40
CGAACAA	15	0.0077513433	50.78846	41
TCCATCC	15	0.0077513433	50.78846	41
CGTTGTA	20	3.7323235E-4	50.78846	39
CGCATTG	30	8.7028457E-7	50.78846	39
ATCGAAC	15	0.0077513433	50.78846	39
GTTGTAG	20	3.7323235E-4	50.78846	40
TCCCTCC	15	0.0077513433	50.78846	41
TTGTTCC	20	3.8042618E-4	50.593872	37
CCCGTTG	20	3.8042618E-4	50.593872	37
TTGTCCA	35	4.3461114E-8	50.59387	37
GGCGCAT	35	4.3461114E-8	50.59387	37
ACCCGTT	20	3.8772976E-4	50.400764	36
TACCCGT	20	3.8772976E-4	50.400764	35

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content