FastQCFastQC Report
Fri 17 Jun 2016
SRR1525917_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525917_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3839
Sequences flagged as poor quality0
Sequence length40
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCATAGGGATGAGATGGACTTAAAGGAATTGACGGGTAC170.44282365199270646No Hit
TTCATAGGGATGAGATGGACTTAAATGAATTGACGGGTAC160.41677520187548844No Hit
TTCATAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT140.36467830164105236No Hit
TTCATAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT130.3386298515238343No Hit
TTCATAGGGATGAGATGGCCTTAAATGAATTGACGGGTAC110.28653295128939826No Hit
TTCATAGGGCTCGTCTTAATCAGCAACATCTTTTGATACA110.28653295128939826No Hit
TTCATAGGGATGAGATGGACTCAAATGAATTGACGGGTAC110.28653295128939826No Hit
TTCATAGGGGCCGAATAGCCTCTCCACCCCAGCGGCCGGA100.26048450117218025No Hit
TTCATAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT90.23443605105496224No Hit
TTCATAGGGATGAGATGGACTCAAAGGAATTGACGGGTAC80.20838760093774422No Hit
TTCATAGGGATGAGATGGCCTTAAAGGAATTGACGGGTAC70.18233915082052618No Hit
TTCATAGGGATGAGATGGCCTCAAATGAATTGACGGGTAC60.15629070070330814No Hit
TTCATAGGGGCCGAATAGCCTCTCCACCCCAGCGGCCCGA60.15629070070330814No Hit
TTCATAGGGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT50.13024225058609012No Hit
TTCATAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.13024225058609012No Hit
TTCATAGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT50.13024225058609012No Hit
TTCATAGGGGAATATTTGGGAACTGAGGGATTGTTCAGTG40.10419380046887211No Hit
TTCATAGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT40.10419380046887211No Hit
TTCATAGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGC40.10419380046887211No Hit
TTCATAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG40.10419380046887211No Hit
TTCATAGGGGTTAAGGACTATGTCCAGGCTAACCCCCGCT40.10419380046887211No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGAATT200.002327423334.3625
AAAGGAA200.002327423334.3623
AAGGAAT200.002327423334.3624
GGAATTG200.002327423334.3626
GATGGCC200.002488112333.90789414
AGGGGGG200.002488112333.9078946
AGGGGAG301.1543096E-533.9078946
GGATGAG301.1543096E-533.9078948
GACGGGT251.7009914E-433.90789432
ATGGCCT200.002488112333.90789415
AGGGCAG200.002488112333.9078946
AGGGATG453.423338E-933.9078946
GATGAGA357.7699224E-733.9078949
AGGGAGT251.7009914E-433.9078946
AGGGACA251.7009914E-433.9078946
AGGGAAG357.7699224E-733.9078946
AGGGAAC251.7009914E-433.9078946
ACGGGTA251.7009914E-433.90789433
TTGACGG251.7009914E-433.90789430
GAATTGA251.7009914E-433.90789427
Produced by FastQC (version 0.11.5)