##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1525917_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3839
Sequences flagged as poor quality	0
Sequence length	40
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.06798645480594	35.0	31.0	38.0	26.0	38.0
2	28.652774159937483	33.0	25.0	38.0	4.0	38.0
3	27.14118259963532	32.0	20.0	37.0	4.0	38.0
4	25.740296952331335	30.0	17.0	36.0	4.0	38.0
5	26.536858556915863	30.0	21.0	35.0	4.0	38.0
6	24.95702005730659	29.0	16.0	35.0	4.0	38.0
7	27.615785360771035	33.0	23.0	36.0	4.0	38.0
8	26.54571502995572	32.0	20.0	36.0	4.0	38.0
9	27.449856733524356	33.0	23.0	36.0	4.0	38.0
10	21.811148736650168	24.0	10.0	33.0	4.0	36.0
11	24.566553790049493	29.0	16.0	34.0	4.0	37.0
12	24.694712164626203	29.0	15.0	35.0	4.0	38.0
13	25.03125814014066	30.0	18.0	35.0	4.0	37.0
14	25.287314404792916	30.0	17.0	35.0	4.0	38.0
15	23.405834852826256	28.0	11.0	34.0	4.0	37.0
16	20.69653555613441	23.0	4.0	32.0	4.0	36.0
17	21.626465225319095	26.0	4.0	33.0	4.0	36.0
18	21.049752539723887	25.0	4.0	32.0	4.0	36.0
19	18.40739775983329	18.0	4.0	30.0	4.0	35.0
20	20.12763740557437	23.0	4.0	32.0	4.0	36.0
21	17.19588434488148	16.0	4.0	29.0	4.0	34.0
22	17.544412607449857	17.0	4.0	30.0	4.0	34.0
23	17.34279760354259	17.0	4.0	29.0	4.0	34.0
24	16.958582964313624	16.0	4.0	29.0	4.0	34.0
25	16.506121385777547	15.0	4.0	29.0	4.0	33.0
26	14.61656681427455	6.0	4.0	27.0	4.0	34.0
27	14.675436311539464	4.0	4.0	27.0	4.0	34.0
28	14.52227142485022	4.0	4.0	27.0	4.0	33.0
29	14.018494399583226	4.0	4.0	26.0	4.0	33.0
30	13.274029695233134	4.0	4.0	24.0	4.0	32.0
31	13.816618911174785	4.0	4.0	27.0	4.0	34.0
32	13.15759312320917	4.0	4.0	26.0	4.0	33.0
33	12.998437092992967	4.0	4.0	26.0	4.0	32.0
34	12.85412867934358	4.0	4.0	26.0	4.0	32.0
35	12.42537119041417	4.0	4.0	24.0	4.0	32.0
36	13.075800989841104	4.0	4.0	27.0	4.0	35.0
37	13.160718937223235	4.0	4.0	29.0	4.0	35.0
38	13.071112268820006	4.0	4.0	29.0	4.0	35.0
39	12.312581401406616	4.0	4.0	25.0	4.0	35.0
40	12.371190414170357	4.0	4.0	26.0	4.0	35.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	-21.760227682670873
1	2	-24.961562868949237
1	3	-23.122796553194277
1	4	-21.328534533648163
1	5	-22.573767299619576
1	6	-20.163711793257253
1	7	-23.553558720320076
1	8	-22.067465236783413
1	9	-22.996881969041056
1	10	-17.369790026892307
1	11	-19.8129431490227
1	12	-19.505705184966544
1	13	-20.309686557129737
1	14	-21.22580266955267
1	15	-18.815142906336092
1	16	-15.734807900432898
1	17	-17.42405098058507
1	18	-16.90210260068215
1	19	-14.166578118850847
1	20	-16.433899055489967
1	21	-12.797791633871189
1	22	-13.145548422537061
1	23	-13.170618112947654
1	24	-12.906040518824607
1	25	-12.453851420044604
1	26	-10.749450265643448
1	27	-10.923595533254625
1	28	-11.027593303161485
1	29	-9.983980224321135
1	30	-9.77543495015086
1	31	-10.182159664830118
1	32	-9.756932556080285
1	33	-9.814494375573922
1	34	-9.757114571035027
1	35	-9.208677685950414
1	36	-10.616025514889152
1	37	-10.605989685819235
1	38	-10.630608848222487
1	39	-9.64898047028729
1	40	-9.59359176177358
6	1	0.9619945395513483
6	2	2.149548242161874
6	3	1.5438701134723907
6	4	-1.8840900892037205
6	5	5.092899367047092
6	6	6.502954873409415
6	7	3.5575523907910345
6	8	4.8214236521054765
6	9	6.558673586514502
6	10	4.18576552866325
6	11	2.409279073199521
6	12	1.6054059261445666
6	13	5.579202331759152
6	14	3.218641774891772
6	15	1.51819042699724
6	16	0.7096365440115449
6	17	2.0203934638593744
6	18	6.097897399317851
6	19	3.7223107700380424
6	20	6.1216565000655905
6	21	11.424430588351033
6	22	7.632229355240717
6	23	11.718270775941235
6	24	9.982848370064282
6	25	9.546148579955396
6	26	5.91721640102322
6	27	9.187515577856486
6	28	8.861295585727405
6	29	9.238241997901087
6	30	8.11345393873803
6	31	2.2622847796143244
6	32	6.576400777253047
6	33	1.185505624426078
6	34	-0.9793367932572483
6	35	0.4579889807162516
6	36	-1.060469959333597
6	37	-4.494878574708125
6	38	1.8138355962219563
6	39	-0.5378693591761792
6	40	-1.70470287288469
7	1	1.8306814082382203
7	2	4.76570985832349
7	3	3.6953852649875394
7	4	6.489647284533653
7	5	5.880778154925878
7	6	9.2908336612882
7	7	6.35553218877083
7	8	7.114352945034767
7	9	7.094027121868038
7	10	9.812028154925876
7	11	4.459784123704573
7	12	2.494294815033456
7	13	4.690313442870263
7	14	3.683288239538239
7	15	1.2757661845729977
7	16	7.628828463203465
7	17	3.5759490194149315
7	18	1.0069883084087614
7	19	4.3788764266037
7	20	5.566100944510033
7	21	8.293117457037901
7	22	7.763542486553849
7	23	7.193018250688709
7	24	10.093959481175393
7	25	5.546148579955396
7	26	-0.8403593565525398
7	27	-1.6508682605273517
7	28	-1.6639569395251215
7	29	0.37965613931522846
7	30	-2.320889495605405
7	31	-2.454886937557392
7	32	-5.3932961924439216
7	33	-5.269039830119377
7	34	-1.9389327528532085
7	35	-5.481404958677687
7	36	-6.161480060343697
7	37	-6.333262413091962
7	38	-5.721517939131578
7	39	-5.285344106650927
7	40	-4.411773579955398
9	1	2.9619945395513483
9	2	4.7051037977174275
9	3	7.654981224583501
9	4	8.227021021907394
9	5	3.3151215892693138
9	6	5.614065984520526
9	7	6.557552390791038
9	8	7.37697920766103
9	9	6.780895808736723
9	10	6.519098861996582
9	11	4.5203901843106316
9	12	5.160961481700124
9	13	2.3569801095369307
9	14	-5.11469155844156
9	15	2.51819042699724
9	16	5.93185876623377
9	17	5.020393463859374
9	18	9.209008510428962
9	19	4.500088547815821
9	20	7.343878722287812
9	21	10.535541699462144
9	22	4.854451577462939
9	23	5.496048553719014
9	24	10.093959481175393
9	25	6.657259691066507
9	26	3.9172164010232198
9	27	3.1875155778564856
9	28	2.3057400301718474
9	29	2.3493531090121973
9	30	5.002342827626919
9	31	2.59561811294766
9	32	5.243067443919715
9	33	8.51883895775941
9	34	7.020663206742752
9	35	4.7913223140495855
9	36	8.050641151777516
9	37	3.1717880919585433
9	38	9.813835596221956
9	39	3.9065750852682672
9	40	6.628630460448642
10	1	2.6488632264200334
10	2	3.8566189492325833
10	3	-1.8500692804670038
10	4	6.489647284533653
10	5	4.4262327003804245
10	6	6.654470024924564
10	7	5.719168552407197
10	8	6.3870802177620405
10	9	6.548572576413491
10	10	6.721119064016783
10	11	2.2779659418863893
10	12	8.130658451397093
10	13	4.23576798832481
10	14	7.0469246031746025
10	15	0.3666752754820912
10	16	11.719737554112555
10	17	10.121403564869478
10	18	10.006988308408761
10	19	12.924330972058243
10	20	11.566100944510033
10	21	16.656753820674265
10	22	17.218087941099306
10	23	16.011200068870526
10	24	15.912141299357213
10	25	13.182512216319033
10	26	3.8869133707201886
10	27	4.440040830381738
10	28	5.3360430604748785
10	29	4.834201593860682
10	30	4.4063832316673235
10	31	2.3632948806244283
10	32	0.9703401711924418
10	33	2.185505624426078
10	34	-0.30256911648957185
10	35	2.064049586776859
10	36	-2.4342073330709706
10	37	0.8485557687262197
10	38	-2.7215179391315782
10	39	-0.9217077430145633
10	40	-2.320864489046306
12	1	4.739772317329127
12	2	8.038437131050763
12	3	8.877203446805723
12	4	3.6714654663518367
12	5	6.4262327003804245
12	6	3.836288206742747
12	7	10.446441279679924
12	8	10.932534763216587
12	9	9.003118030958944
12	10	14.630209973107693
12	11	13.1870568509773
12	12	7.494294815033456
12	13	6.690313442870263
12	14	11.774197330447329
12	15	0.1848570936639078
12	16	8.265192099567102
12	17	11.575949019414931
12	18	12.097897399317851
12	19	11.833421881149153
12	20	12.566100944510033
12	21	-1.7977916338711886
12	22	12.854451577462939
12	23	4.829381887052346
12	24	14.093959481175393
12	25	-12.453851420044604
12	26	-10.749450265643448
12	27	-10.923595533254625
12	28	-11.027593303161485
12	29	-9.983980224321135
12	30	-9.77543495015086
12	31	-10.182159664830118
12	32	-9.756932556080285
12	33	-9.814494375573922
12	34	-9.757114571035027
12	35	-9.208677685950414
12	36	-10.616025514889152
12	37	-10.605989685819235
12	38	-10.630608848222487
12	39	-9.64898047028729
12	40	-9.59359176177358
13	1	-12.169318591761783
13	2	-16.3251992325856
13	3	-11.850069280467004
13	4	-16.14671635182998
13	5	-12.391949117801394
13	6	-11.709166338711798
13	7	-11.553558720320076
13	8	-9.61291978223796
13	9	-11.087791059950147
13	10	-7.006153663255944
13	11	-3.9038522399317905
13	12	-0.9602506395119974
13	13	-3.7642320116751904
13	14	-2.4076208513708544
13	15	-1.9969610881542756
13	16	1.4470102813852854
13	17	-0.4240509805850685
13	18	0.5524428538633046
13	19	0.5606946084218798
13	20	-3.9793536009445134
13	21	-2.070518906598462
13	22	-0.6000938770825144
13	23	-0.44334538567492743
13	24	-0.1787677915518806
13	25	-0.9993059654991487
13	26	-5.203995720188903
13	27	-6.014504624163717
13	28	-8.663956939525121
13	29	-4.7112529515938615
13	30	-4.957253131969042
13	31	-4.727614210284663
13	32	-5.4842052833530115
13	33	-6.0872216483011945
13	34	-5.302569116489572
13	35	-4.845041322314051
13	36	-3.4342073330709706
13	37	-4.06053514036469
13	38	-2.6306088482224865
13	39	-1.8307986521054715
13	40	-2.1390463072281243
14	1	-0.926894349337541
14	2	1.3717704643840953
14	3	-11.789463219860943
14	4	5.671465466351837
14	5	-0.9071006329529077
14	6	-0.4970451265905851
14	7	4.446441279679924
14	8	6.5992014298832515
14	9	4.0031180309589445
14	10	8.29687663977436
14	11	4.5203901843106316
14	12	7.827628148366788
14	13	2.690313442870263
14	14	3.774197330447329
14	15	-2.1484762396694244
14	16	7.265192099567102
14	17	10.909282352748267
14	18	11.764564065984516
14	19	14.500088547815817
14	20	13.899434277843369
14	21	4.202208366128811
14	22	-1.8122150892037272
14	23	4.162715220385678
14	24	-1.5727071854912733
14	25	-1.1205180867112698
14	26	3.9172164010232198
14	27	6.076404466745375
14	28	-3.027593303161485
14	29	0.34935310901219907
14	30	0.557898383182474
14	31	6.484507001836549
14	32	2.5764007772530473
14	33	7.852172291092746
14	34	-0.7571145710350269
14	35	6.457988980716253
14	36	3.3839744851108478
14	37	8.727343647514097
14	38	8.702724485110846
14	39	0.3510195297127101
14	40	6.406408238226421
15	1	3.739772317329127
15	2	6.438437131050762
15	3	7.877203446805723
15	4	1.4714654663518374
15	5	8.226232700380422
15	6	8.836288206742747
15	7	8.646441279679927
15	8	10.13253476321659
15	9	9.203118030958947
15	10	8.630209973107693
15	11	10.1870568509773
15	12	5.094294815033457
15	13	8.690313442870263
15	14	10.37419733044733
15	15	6.584857093663906
15	16	8.6651920995671
15	17	12.375949019414929
15	18	7.297897399317851
15	19	12.633421881149154
15	20	8.766100944510033
15	21	7.802208366128813
15	22	6.2544515774629375
15	23	12.029381887052345
15	24	13.093959481175393
15	25	9.346148579955397
15	26	12.050549734356553
15	27	0.2764044667453742
15	28	3.1724066968385145
15	29	1.6160197756788648
15	30	-0.1754349501508603
15	31	-1.5821596648301188
15	32	3.043067443919716
15	33	3.5855056244260766
15	34	6.442885428964972
15	35	4.991322314049585
15	36	5.783974485110846
15	37	4.994010314180766
15	38	4.969391151777515
15	39	0.15101952971271082
15	40	5.006408238226422
16	1	3.614772317329127
16	2	5.913437131050763
16	3	7.252203446805723
16	4	8.546465466351837
16	5	7.0512327003804245
16	6	5.961288206742747
16	7	7.696441279679924
16	8	9.307534763216587
16	9	5.2531180309589445
16	10	10.255209973107693
16	11	7.812056850977299
16	12	7.369294815033456
16	13	8.565313442870263
16	14	3.899197330447329
16	15	4.559857093663908
16	16	8.890192099567102
16	17	11.700949019414931
16	18	12.097897399317851
16	19	8.333421881149153
16	20	10.316100944510033
16	21	1.3272083661288114
16	22	0.35445157746293887
16	23	3.454381887052346
16	24	2.3439594811753928
16	25	-1.2038514200446038
16	26	1.625549734356552
16	27	4.201404466745375
16	28	0.22240669683851522
16	29	3.641019775678865
16	30	2.59956504984914
16	31	5.0678403351698815
16	32	5.868067443919715
16	33	5.060505624426078
16	34	7.117885428964973
16	35	5.1663223140495855
16	36	4.133974485110848
16	37	1.7690103141807647
16	38	2.7443911517775135
16	39	5.35101952971271
16	40	1.2814082382264207
17	1	4.639772317329125
17	2	7.538437131050763
17	3	8.277203446805721
17	4	7.571465466351835
17	5	5.4262327003804245
17	6	9.336288206742747
17	7	8.346441279679922
17	8	8.532534763216589
17	9	8.703118030958947
17	10	9.230209973107694
17	11	7.587056850977298
17	12	10.094294815033457
17	13	9.090313442870261
17	14	7.974197330447332
17	15	9.684857093663908
17	16	7.065192099567103
17	17	11.175949019414933
17	18	9.29789739931785
17	19	10.233421881149152
17	20	11.566100944510033
17	21	4.502208366128812
17	22	11.95445157746294
17	23	12.029381887052345
17	24	12.193959481175394
17	25	8.046148579955396
17	26	7.550549734356553
17	27	10.976404466745374
17	28	8.872406696838514
17	29	8.616019775678867
17	30	5.8245650498491415
17	31	8.417840335169883
17	32	7.443067443919714
17	33	8.685505624426078
17	34	8.742885428964973
17	35	4.591322314049586
17	36	4.883974485110848
17	37	5.194010314180765
17	38	5.169391151777514
17	39	6.4510195297127115
17	40	6.006408238226422
18	1	2.9619945395513483
18	2	5.038437131050763
18	3	7.2105367801390585
18	4	7.449243244129615
18	5	6.537343811491539
18	6	3.836288206742747
18	7	7.224219057457702
18	8	7.5992014298832515
18	9	7.558673586514502
18	10	10.074654417552136
18	11	7.29816796208841
18	12	6.827628148366788
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102	34	-3.507114571035027
102	35	-1.9586776859504145
102	36	-1.8660255148891522
102	37	-3.1059896858192353
102	38	-3.1306088482224865
102	39	-2.14898047028729
102	40	-4.593591761773579
103	1	3.739772317329127
103	2	-0.21156286894923682
103	3	1.6272034468057228
103	4	3.4214654663518367
103	5	1.6762327003804245
103	6	2.836288206742747
103	7	-0.30355872032007625
103	8	-6.067465236783413
103	9	0.2531180309589445
103	10	-4.619790026892307
103	11	1.4370568509772994
103	12	-0.005705184966544152
103	13	-2.809686557129737
103	14	-0.22580266955267092
103	15	-1.3151429063360922
103	16	4.515192099567102
103	17	4.3259490194149315
103	18	3.8478973993178514
103	19	-2.166578118850847
103	20	3.8161009445100333
103	21	-12.797791633871189
103	22	-13.145548422537061
103	23	-13.170618112947654
103	24	-12.906040518824607
103	25	-12.453851420044604
103	26	-10.749450265643448
103	27	-10.923595533254625
103	28	-11.027593303161485
103	29	-9.983980224321135
103	30	-9.77543495015086
103	31	-10.182159664830118
103	32	-9.756932556080285
103	33	-9.814494375573922
103	34	-9.757114571035027
103	35	-9.208677685950414
103	36	-10.616025514889152
103	37	-10.605989685819235
103	38	-10.630608848222487
103	39	-9.64898047028729
103	40	-9.59359176177358
104	1	2.989772317329127
104	2	5.038437131050763
104	3	6.377203446805723
104	4	2.9214654663518367
104	5	4.9262327003804245
104	6	4.836288206742747
104	7	4.696441279679924
104	8	4.932534763216587
104	9	-0.9968819690410555
104	10	-0.6197900268923071
104	11	7.937056850977299
104	12	5.244294815033456
104	13	5.690313442870263
104	14	6.024197330447329
104	15	10.184857093663908
104	16	3.515192099567102
104	17	7.0759490194149315
104	18	5.597897399317851
104	19	8.083421881149153
104	20	5.066100944510033
104	21	-0.047791633871188566
104	22	1.8544515774629389
104	23	-2.920618112947654
104	24	1.3439594811753928
104	25	6.546148579955396
104	26	-0.24945026564344808
104	27	3.326404466745375
104	28	5.472406696838515
104	29	4.516019775678865
104	30	5.72456504984914
104	31	3.3178403351698815
104	32	7.743067443919715
104	33	9.935505624426078
104	34	9.742885428964973
104	35	11.541322314049586
104	36	10.383974485110848
104	37	9.644010314180765
104	38	8.869391151777513
104	39	10.10101952971271
104	40	8.15640823822642
105	1	3.739772317329127
105	2	7.038437131050763
105	3	8.127203446805723
105	4	5.421465466351837
105	5	6.4262327003804245
105	6	4.336288206742747
105	7	7.696441279679924
105	8	9.932534763216587
105	9	7.2531180309589445
105	10	9.380209973107693
105	11	8.9370568509773
105	12	11.994294815033456
105	13	9.190313442870263
105	14	10.774197330447329
105	15	5.684857093663908
105	16	4.765192099567102
105	17	5.5759490194149315
105	18	4.097897399317851
105	19	6.083421881149153
105	20	1.3161009445100333
105	21	-5.297791633871189
105	22	-4.895548422537061
105	23	-7.920618112947654
105	24	0.5939594811753928
105	25	3.2961485799553962
105	26	-1.249450265643448
105	27	1.576404466745375
105	28	5.222406696838515
105	29	-1.2339802243211349
105	30	1.22456504984914
105	31	3.3178403351698815
105	32	7.993067443919715
105	33	6.685505624426078
105	34	4.742885428964973
105	35	6.5413223140495855
105	36	11.883974485110848
105	37	11.644010314180765
105	38	10.369391151777513
105	39	1.60101952971271
105	40	8.40640823822642
106	1	4.239772317329127
106	2	6.538437131050763
106	3	8.877203446805723
106	4	7.671465466351837
106	5	3.9262327003804245
106	6	-0.6637117932572529
106	7	3.4464412796799238
106	8	6.432534763216587
106	9	5.0031180309589445
106	10	-0.3697900268923071
106	11	8.9370568509773
106	12	7.244294815033456
106	13	5.440313442870263
106	14	6.524197330447329
106	15	-1.0651429063360922
106	16	8.765192099567102
106	17	10.825949019414931
106	18	6.097897399317851
106	19	14.583421881149153
106	20	13.566100944510033
106	21	-6.297791633871189
106	22	-5.895548422537061
106	23	-9.670618112947654
106	24	-7.656040518824607
106	25	-5.953851420044604
106	26	-2.249450265643448
106	27	-2.423595533254625
106	28	-2.527593303161485
106	29	-2.733980224321135
106	30	-2.02543495015086
106	31	-2.1821596648301185
106	32	-3.256932556080285
106	33	-5.064494375573922
106	34	-1.7571145710350269
106	35	-4.2086776859504145
106	36	-6.866025514889152
106	37	-7.105989685819235
106	38	-6.6306088482224865
106	39	-8.14898047028729
106	40	-3.0935917617735793
107	1	4.406438983995791
107	2	4.038437131050763
107	3	9.543870113472387
107	4	-4.6618678669814955
107	5	5.75956603371376
107	6	7.502954873409415
107	7	6.7797746130132595
107	8	7.5992014298832515
107	9	7.33645136429228
107	10	6.296876639774361
107	11	9.520390184310635
107	12	8.494294815033456
107	13	9.690313442870263
107	14	4.774197330447329
107	15	12.184857093663908
107	16	13.265192099567102
107	17	4.909282352748264
107	18	12.431230732651187
107	19	-3.4999114521841808
107	20	1.8994342778433655
107	21	-9.13112496720452
107	22	-11.478881755870393
107	23	-7.83728477961432
107	24	-6.239373852157941
107	25	-3.4538514200446038
107	26	-2.749450265643448
107	27	-3.590262199921291
107	28	-2.3609266364948187
107	29	-5.983980224321135
107	30	0.22456504984914005
107	31	-10.182159664830118
107	32	-9.756932556080285
107	33	-9.814494375573922
107	34	-9.757114571035027
107	35	-9.208677685950414
107	36	-10.616025514889152
107	37	-10.605989685819235
107	38	-10.630608848222487
107	39	-9.64898047028729
107	40	-9.59359176177358
108	1	-1.5935610160042053
108	2	1.0384371310507632
108	3	2.877203446805723
108	4	-1.3285345336481633
108	5	0.4262327003804245
108	6	-9.163711793257253
108	7	2.1131079463465916
108	8	3.2658680965499194
108	9	2.6697846976256123
108	10	-3.036456693558975
108	11	3.1870568509772994
108	12	1.4942948150334558
108	13	1.023646776203595
108	14	4.774197330447329
108	15	7.851523760330576
108	16	1.5985254329004341
108	17	3.2426156860815993
108	18	2.4312307326511835
108	19	7.500088547815821
108	20	-2.4338990554899667
108	21	-4.1311249672045225
108	22	0.5211182441296067
108	23	0.829381887052346
108	24	3.427292814508725
108	25	2.212815246622064
108	26	9.583883067689884
108	27	6.076404466745375
108	28	6.305740030171847
108	29	11.682686442345533
108	30	11.557898383182472
108	31	10.48450700183655
108	32	7.576400777253047
108	33	13.185505624426078
108	34	4.242885428964973
108	35	8.791322314049586
108	36	9.383974485110848
108	37	2.3940103141807647
108	38	9.369391151777513
108	39	10.35101952971271
108	40	10.40640823822642
110	1	3.739772317329127
110	2	7.038437131050763
110	3	8.877203446805723
110	4	4.671465466351837
110	5	8.426232700380424
110	6	11.836288206742747
110	7	8.446441279679924
110	8	9.932534763216587
110	9	9.003118030958944
110	10	7.630209973107693
110	11	2.1870568509772994
110	12	5.494294815033456
110	13	5.690313442870263
110	14	6.774197330447329
110	15	9.184857093663908
110	16	-7.734807900432898
110	17	5.5759490194149315
110	18	-4.902102600682149
110	19	-5.166578118850847
110	20	6.566100944510033
110	21	16.20220836612881
110	22	17.85445157746294
110	23	10.829381887052346
110	24	12.093959481175393
110	25	11.546148579955396
110	26	20.250549734356554
110	27	20.076404466745373
110	28	19.972406696838515
110	29	21.016019775678863
110	30	22.22456504984914
110	31	15.817840335169882
110	32	16.243067443919713
110	33	-0.814494375573922
110	34	16.242885428964975
110	35	16.791322314049587
110	36	2.3839744851108478
110	37	12.394010314180765
110	38	2.3693911517775135
110	39	3.35101952971271
110	40	3.4064082382264207
112	1	-9.010227682670873
112	2	-19.711562868949237
112	3	-3.872796553194277
112	4	-2.8285345336481633
112	5	-9.073767299619576
112	6	-12.163711793257253
112	7	-7.053558720320076
112	8	-7.817465236783413
112	9	-4.7468819690410555
112	10	-9.869790026892307
112	11	-1.5629431490227006
112	12	0.24429481503345585
112	13	0.44031344287026286
112	14	-0.4758026695526709
112	15	-1.3151429063360922
112	16	-1.734807900432898
112	17	0.5759490194149315
112	18	-3.9021026006821486
112	19	-4.416578118850847
112	20	-3.9338990554899667
112	21	-5.297791633871189
112	22	-2.145548422537061
112	23	-5.670618112947654
112	24	-5.156040518824607
112	25	-1.2038514200446038
112	26	-3.249450265643448
112	27	-3.423595533254625
112	28	-5.027593303161485
112	29	-4.233980224321135
112	30	3.47456504984914
112	31	0.8178403351698815
112	32	1.9930674439197151
112	33	-0.814494375573922
112	34	7.992885428964973
112	35	3.7913223140495855
112	36	13.883974485110848
112	37	12.394010314180765
112	38	13.119391151777513
112	39	11.85101952971271
112	40	13.40640823822642
114	1	0.7397723173291268
114	2	4.038437131050763
114	3	-5.122796553194277
114	4	-7.328534533648163
114	5	4.4262327003804245
114	6	8.836288206742747
114	7	7.446441279679924
114	8	8.932534763216587
114	9	6.0031180309589445
114	10	11.630209973107693
114	11	9.1870568509773
114	12	-10.505705184966544
114	13	6.690313442870263
114	14	5.774197330447329
114	15	6.184857093663908
114	16	-4.734807900432898
114	17	11.575949019414931
114	18	4.097897399317851
114	19	14.833421881149153
114	20	7.566100944510033
114	21	1.2022083661288114
114	22	-8.145548422537061
114	23	15.829381887052346
114	24	-6.906040518824607
114	25	16.546148579955396
114	26	-10.749450265643448
114	27	-10.923595533254625
114	28	-11.027593303161485
114	29	-9.983980224321135
114	30	-9.77543495015086
114	31	-10.182159664830118
114	32	-9.756932556080285
114	33	-9.814494375573922
114	34	-9.757114571035027
114	35	-9.208677685950414
114	36	-10.616025514889152
114	37	-10.605989685819235
114	38	-10.630608848222487
114	39	-9.64898047028729
114	40	-9.59359176177358
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
4	489.0
5	54.0
6	31.0
7	22.0
8	13.0
9	35.0
10	77.0
11	78.0
12	76.0
13	94.0
14	126.0
15	139.0
16	134.0
17	130.0
18	140.0
19	151.0
20	150.0
21	146.0
22	169.0
23	152.0
24	185.0
25	208.0
26	205.0
27	177.0
28	183.0
29	148.0
30	113.0
31	86.0
32	55.0
33	45.0
34	13.0
35	12.0
36	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	100.0	0.0	0.0
7	99.47807933194154	0.33924843423799583	0.1304801670146138	0.052192066805845504
8	98.20125130344108	0.49530761209593327	0.5213764337851928	0.7820646506777894
9	97.18236368379858	1.5131750587007564	0.8609444299504304	0.4435168275502218
10	30.059911435269605	42.172440739775986	11.539463401927586	16.228184423026832
11	22.089085699400883	34.644438655899975	25.136754363115394	18.12972128158375
12	43.18833029434749	17.218025527481114	18.286011982287054	21.307632195884345
13	26.517322219327948	23.59989580619953	23.73013805678562	26.152643917686895
14	27.97603542589216	30.08595988538682	23.547798905965095	18.390205782755924
15	24.563688460536596	27.246678822610054	27.090388121906745	21.0992445949466
16	24.2771555092472	20.135451940609535	31.961448293826518	23.62594425631675
17	30.6329773378484	23.75618650690284	22.27142485022141	23.33941130502735
18	27.533211773899453	25.006512112529304	21.20343839541547	26.25683771815577
19	18.500130650640187	20.17245884504834	18.055918474000524	43.271492030310945
20	19.953112789789007	24.38134930971607	23.834331857254494	31.831206043240428
21	20.135451940609535	18.98932013545194	33.70669445168012	27.1685334722584
22	20.03125814014066	18.780932534514196	26.439176868976293	34.748632456368846
23	24.042719458192238	26.64756446991404	27.793696275071632	21.51601979682209
24	18.23391508205262	25.47538421463923	25.761917165928626	30.528783537379528
25	21.51238591916558	29.308996088657107	24.11994784876141	25.058670143415906
26	21.07470511140236	22.621231979030142	32.16251638269987	24.14154652686763
27	24.38134930971607	19.90101588955457	30.08595988538682	25.63167491534254
28	18.83302943474863	22.115134149518102	33.44620995050794	25.605626465225317
29	14.337851929092803	23.592283628779978	35.870698644421275	26.19916579770594
30	14.553990610328638	17.136150234741784	43.53155972874283	24.778299426186752
31	12.60438413361169	21.477035490605427	47.80793319415449	18.11064718162839
32	19.90101588955457	15.498827819744726	43.6311539463402	20.96900234436051
33	11.617608752279239	20.00520969002344	45.194060953373274	23.183120604324042
34	12.086480854389164	17.71294607970826	49.64834592341756	20.552227142485023
35	16.831683168316832	16.935904116727464	48.51485148514851	17.717561229807192
36	13.678999478895257	17.587285044293903	49.66128191766545	19.072433559145388
37	12.669447340980188	16.579770594369133	49.6089676746611	21.141814389989573
38	10.784058348528262	18.10367283146653	51.49778588174003	19.614482938265173
39	11.620635747785304	21.547681083897864	48.4627410109432	18.36894215737363
40	9.195102891377962	22.010940349049232	48.189632716853346	20.60432404271946
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	1.0
11	5.0
12	9.0
13	9.0
14	9.0
15	9.0
16	24.0
17	39.0
18	39.0
19	42.5
20	46.0
21	55.5
22	65.0
23	65.0
24	73.5
25	82.0
26	102.5
27	123.0
28	123.0
29	159.0
30	195.0
31	209.5
32	224.0
33	224.0
34	255.5
35	287.0
36	287.5
37	288.0
38	288.0
39	308.0
40	328.0
41	313.0
42	298.0
43	298.0
44	292.0
45	286.0
46	266.5
47	247.0
48	247.0
49	249.5
50	252.0
51	230.5
52	209.0
53	209.0
54	218.0
55	227.0
56	187.0
57	147.0
58	147.0
59	140.5
60	134.0
61	124.0
62	114.0
63	114.0
64	109.5
65	105.0
66	86.5
67	68.0
68	68.0
69	51.0
70	34.0
71	24.0
72	14.0
73	14.0
74	8.5
75	3.0
76	2.5
77	2.0
78	2.0
79	2.5
80	3.0
81	1.5
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.18233915082052618
8	0.07814535035165407
9	0.15629070070330814
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.3125814014066163
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.10419380046887211
26	0.6251628028132326
27	0.0
28	0.0
29	0.07814535035165407
30	0.13024225058609012
31	0.18233915082052618
32	0.0
33	0.0
34	0.0
35	0.026048450117218028
36	0.026048450117218028
37	0.07814535035165407
38	0.0
39	0.026048450117218028
40	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
40	3839.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	92.471997916124
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.05633802816902	88.82521489971347
2	2.873239436619718	5.313883823912477
3	0.47887323943661975	1.3284709559781192
4	0.14084507042253522	0.5209690023443605
5	0.08450704225352113	0.3907267517582704
6	0.056338028169014086	0.3125814014066163
7	0.028169014084507043	0.18233915082052618
8	0.028169014084507043	0.20838760093774422
9	0.028169014084507043	0.23443605105496224
>10	0.22535211267605634	2.6829903620734568
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TTCATAGGGATGAGATGGACTTAAAGGAATTGACGGGTAC	17	0.44282365199270646	No Hit
TTCATAGGGATGAGATGGACTTAAATGAATTGACGGGTAC	16	0.41677520187548844	No Hit
TTCATAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT	14	0.36467830164105236	No Hit
TTCATAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT	13	0.3386298515238343	No Hit
TTCATAGGGATGAGATGGCCTTAAATGAATTGACGGGTAC	11	0.28653295128939826	No Hit
TTCATAGGGCTCGTCTTAATCAGCAACATCTTTTGATACA	11	0.28653295128939826	No Hit
TTCATAGGGATGAGATGGACTCAAATGAATTGACGGGTAC	11	0.28653295128939826	No Hit
TTCATAGGGGCCGAATAGCCTCTCCACCCCAGCGGCCGGA	10	0.26048450117218025	No Hit
TTCATAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT	9	0.23443605105496224	No Hit
TTCATAGGGATGAGATGGACTCAAAGGAATTGACGGGTAC	8	0.20838760093774422	No Hit
TTCATAGGGATGAGATGGCCTTAAAGGAATTGACGGGTAC	7	0.18233915082052618	No Hit
TTCATAGGGATGAGATGGCCTCAAATGAATTGACGGGTAC	6	0.15629070070330814	No Hit
TTCATAGGGGCCGAATAGCCTCTCCACCCCAGCGGCCCGA	6	0.15629070070330814	No Hit
TTCATAGGGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT	5	0.13024225058609012	No Hit
TTCATAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5	0.13024225058609012	No Hit
TTCATAGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT	5	0.13024225058609012	No Hit
TTCATAGGGGAATATTTGGGAACTGAGGGATTGTTCAGTG	4	0.10419380046887211	No Hit
TTCATAGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	4	0.10419380046887211	No Hit
TTCATAGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGC	4	0.10419380046887211	No Hit
TTCATAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG	4	0.10419380046887211	No Hit
TTCATAGGGGTTAAGGACTATGTCCAGGCTAACCCCCGCT	4	0.10419380046887211	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.026048450117218024	0.0	0.0	0.0	0.0
28	0.026048450117218024	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGAATT	20	0.0023274233	34.36	25
AAAGGAA	20	0.0023274233	34.36	23
AAGGAAT	20	0.0023274233	34.36	24
GGAATTG	20	0.0023274233	34.36	26
GATGGCC	20	0.0024881123	33.907894	14
AGGGGGG	20	0.0024881123	33.907894	6
AGGGGAG	30	1.1543096E-5	33.907894	6
GGATGAG	30	1.1543096E-5	33.907894	8
GACGGGT	25	1.7009914E-4	33.907894	32
ATGGCCT	20	0.0024881123	33.907894	15
AGGGCAG	20	0.0024881123	33.907894	6
AGGGATG	45	3.423338E-9	33.907894	6
GATGAGA	35	7.7699224E-7	33.907894	9
AGGGAGT	25	1.7009914E-4	33.907894	6
AGGGACA	25	1.7009914E-4	33.907894	6
AGGGAAG	35	7.7699224E-7	33.907894	6
AGGGAAC	25	1.7009914E-4	33.907894	6
ACGGGTA	25	1.7009914E-4	33.907894	33
TTGACGG	25	1.7009914E-4	33.907894	30
GAATTGA	25	1.7009914E-4	33.907894	27
>>END_MODULE