FastQCFastQC Report
Fri 17 Jun 2016
SRR1525916_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525916_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1017
Sequences flagged as poor quality0
Sequence length40
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCATAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT60.5899705014749262No Hit
TTCATAGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT40.39331366764995085No Hit
TTCATAGGGCGTCGACTTTTTTTTTTTTTTTTTTTTTTTT30.2949852507374631No Hit
TTCATAGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.2949852507374631No Hit
TTCATAGGGACCAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.19665683382497542No Hit
TTCATAGGGACCTCTTGATTAGCATAAACCATTTTTTTAT20.19665683382497542No Hit
TTCATAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.19665683382497542No Hit
TTCATAGGGATGAGATGGACTTAAATGAATTGACGGGTAC20.19665683382497542No Hit
TTCATAGGAGGGTCGACTTTTTTTTTTTTTTTTTTTTTTT20.19665683382497542No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG401.0568328E-834.04
ATAGGGC200.001662030434.04
TTCATAG1000.032.31
TCATAGG1000.032.32
CATAGGG900.032.111113
ATAGGGA250.00497942727.1999994
Produced by FastQC (version 0.11.5)