FastQCFastQC Report
Fri 17 Jun 2016
SRR1525914_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525914_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27810
Sequences flagged as poor quality0
Sequence length56
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCATGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT3101.1147069399496585No Hit
CTCCATGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC2450.8809780654440849No Hit
CTCCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG1310.4710535778496943No Hit
CTCCATGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC760.2732829917295937No Hit
CTCCATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT720.2588996763754045No Hit
CTCCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC700.25170801869830994No Hit
CTCCATGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT690.24811218985976266No Hit
CTCCATGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT670.2409205321826681No Hit
CTCCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC600.2157497303128371No Hit
CTCCATGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA490.17619561308881698No Hit
CTCCATGGGAGAAATAAACGCTTGAATACATCCAGCCTTGCTTATTTCAAATATTT430.15462064005753326No Hit
CTCCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG400.1438331535418914No Hit
CTCCATGGGGCTCTCCATGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAA400.1438331535418914No Hit
CTCCATGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCTC350.12585400934915497No Hit
CTCCATGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCC340.1222581805106077No Hit
CTCCATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA330.11866235167206042No Hit
CTCCATGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT300.10787486515641855No Hit
CTCCATGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC300.10787486515641855No Hit
CTCCATGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA280.10068320747932398No Hit
CTCCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC280.10068320747932398No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTCCG150.00814635750.30489742
CCTATCC150.0082051150.21376840
TCCTATC150.0082051150.21376839
TTTCCTA150.0082051150.21376837
GGCGCAT150.0082051150.21376837
TTCCTAT150.0082051150.21376838
GCGCATT150.0082051150.21376838
CGCATTG150.0082051150.21376839
CAATGGC150.00832356250.0324915
TTCAATG150.00832356250.0324913
CATCCTC150.00832356250.0324915
AGAACGC150.00832356250.0324948
ACTCATC150.00832356250.0324950
GACGAGA150.00832356250.0324914
TGAGGGT150.00832356250.0324949
CAACATT150.00838326349.9423429
CCTTGAC150.00838326349.9423429
CTTGACC150.00838326349.9423430
CGGTTCG150.00838326349.9423430
CCGGTTC150.00838326349.9423429
Produced by FastQC (version 0.11.5)