##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1525914_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 27810 Sequences flagged as poor quality 0 Sequence length 56 %GC 52 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.16033800791082 39.0 38.0 40.0 33.0 40.0 2 36.009097446961526 39.0 36.0 40.0 27.0 40.0 3 36.04041711614527 39.0 36.0 40.0 27.0 40.0 4 36.140489032722044 39.0 36.0 40.0 27.0 40.0 5 34.71157856886012 39.0 35.0 40.0 20.0 40.0 6 35.94710535778497 39.0 36.0 40.0 27.0 40.0 7 36.71995685005394 39.0 37.0 40.0 31.0 40.0 8 36.427328299172956 39.0 36.0 40.0 30.0 40.0 9 36.321251348435815 39.0 36.0 40.0 30.0 40.0 10 35.2720244516361 38.0 35.0 40.0 26.0 40.0 11 35.44663790003596 38.0 35.0 40.0 28.0 40.0 12 34.239661992089175 38.0 33.0 39.0 23.0 40.0 13 33.60963682128731 38.0 32.0 39.0 20.0 40.0 14 34.30859403092413 38.0 33.0 39.0 23.0 40.0 15 33.17472132326501 38.0 32.0 39.0 18.0 40.0 16 32.433656957928804 36.0 29.0 39.0 18.0 40.0 17 31.405717367853292 36.0 27.0 39.0 15.0 40.0 18 30.82391226177634 35.0 26.0 39.0 13.0 40.0 19 32.02250988852931 36.0 29.0 39.0 17.0 40.0 20 32.64775260697591 36.0 30.0 39.0 20.0 40.0 21 32.92829917295937 38.0 30.0 39.0 19.0 40.0 22 32.14271844660194 37.0 29.0 39.0 16.0 40.0 23 32.910787486515645 38.0 30.0 39.0 19.0 40.0 24 32.29841783531104 37.0 29.0 39.0 17.0 40.0 25 32.70560949298813 37.0 30.0 39.0 18.0 40.0 26 33.19644012944984 38.0 32.0 39.0 18.0 40.0 27 33.451420352391224 38.0 32.0 39.0 20.0 40.0 28 33.45454872348076 38.0 32.0 39.0 21.0 40.0 29 32.942718446601944 38.0 31.0 39.0 18.0 40.0 30 32.756274721323265 37.0 31.0 39.0 18.0 40.0 31 33.55070118662351 38.0 33.0 39.0 20.0 40.0 32 33.39978425026969 38.0 33.0 39.0 20.0 40.0 33 33.227112549442644 38.0 33.0 39.0 19.0 40.0 34 33.05976267529665 37.0 32.0 39.0 18.0 40.0 35 33.093275800071915 37.0 33.0 39.0 18.0 40.0 36 32.2316792520676 36.0 31.0 39.0 15.0 40.0 37 32.010607695073716 36.0 31.0 39.0 12.0 40.0 38 31.470478245235526 36.0 30.0 38.0 4.0 40.0 39 30.523948220064725 35.0 28.0 38.0 4.0 40.0 40 28.093923049262855 34.0 20.0 38.0 4.0 40.0 41 26.229809421071558 31.0 16.0 37.0 4.0 39.0 42 27.151456310679613 32.0 20.0 37.0 4.0 39.0 43 27.10427903631787 32.0 20.0 38.0 4.0 39.0 44 26.265623876303486 31.0 17.0 37.0 4.0 39.0 45 27.24041711614527 32.0 21.0 38.0 4.0 39.0 46 25.231643293779218 30.0 16.0 36.0 4.0 39.0 47 24.223085221143474 28.0 10.0 36.0 4.0 39.0 48 24.89539733908666 29.0 15.0 36.0 4.0 39.0 49 23.773282991729594 28.0 8.0 35.0 4.0 39.0 50 24.575764113628193 29.0 11.0 36.0 4.0 39.0 51 24.765408126573174 30.0 4.0 36.0 4.0 39.0 52 24.638727076591156 30.0 4.0 36.0 4.0 39.0 53 24.453110391945344 30.0 4.0 36.0 4.0 39.0 54 24.13042071197411 30.0 4.0 36.0 4.0 39.0 55 23.907119741100324 30.0 4.0 36.0 4.0 39.0 56 23.20151024811219 29.0 4.0 36.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 2.875625699621537 1 2 4.278103796072443 1 3 4.511183347755356 1 4 4.42518186249481 1 5 6.46234036225156 1 6 4.3101292951154235 1 7 3.3922444736681996 1 8 3.4693043143769913 1 9 3.7822406912805846 1 10 5.076006865030976 1 11 -0.0765680475894257 1 12 -7.184417025095904 1 13 -18.604279915633686 1 14 -7.203944431264709 1 15 1.1598487842543364 1 16 -2.5248539797410494 1 17 -7.124699447745304 1 18 -5.05099626981977 1 19 -0.34625824230429814 1 20 -1.340863087070968 1 21 6.7388741919708295 1 22 7.3302305962562 1 23 6.594303783283355 1 24 7.162388830017676 1 25 6.394477768287679 1 26 6.149216823218499 1 27 5.750079850428019 1 28 5.368798698386605 1 29 6.225935356324332 1 30 6.427717988217129 1 31 5.781733573491753 1 32 5.533439225012803 1 33 5.600381145368658 1 34 6.514818617251887 1 35 6.367205081003796 1 36 4.955419284631713 1 37 5.595589531013772 1 38 5.735765188764162 1 39 6.367462331127292 1 40 -11.194224036213921 1 41 -1.6889884500878445 1 42 -3.0466498052956723 1 43 -10.899504133515306 1 44 -15.665440052569013 1 45 -3.609916536438366 1 46 -7.136253861557217 1 47 -12.43963165589463 1 48 -6.070795577457879 1 49 -14.012283649178457 1 50 -4.881087321046532 1 51 0.9859346843678622 1 52 0.5140402106045059 1 53 1.0786158956421161 1 54 0.8269290416172694 1 55 1.2746559724796747 1 56 3.9167105122796 2 1 3.331099324125219 2 2 4.631478725170567 2 3 4.505511198010602 2 4 4.6191693837653744 2 5 6.675045977679801 2 6 4.124649998461997 2 7 3.8244622842183915 2 8 3.3558613194819245 2 9 4.156602574434295 2 10 4.571185537747937 2 11 3.3369316688030892 2 12 1.1145052664525963 2 13 2.350910101382766 2 14 4.777904689552081 2 15 5.022015545456831 2 16 4.417290092862469 2 17 8.118068561330134 2 18 5.610376390418459 2 19 7.061002109368989 2 20 6.0652628346533675 2 21 7.563037549883475 2 22 7.9224030296084464 2 23 7.209732030589084 2 24 8.019450656449898 2 25 6.994023996308101 2 26 7.009681939497572 2 27 6.638338500456374 2 28 5.728980207178438 2 29 6.858947267838797 2 30 6.527547823724792 2 31 5.206010374399302 2 32 6.132418238058744 2 33 5.700210980876321 2 34 5.145561668868449 2 35 5.769360497906803 2 36 6.799435166650998 2 37 6.871256008608782 2 38 7.858850838225308 2 39 6.883627957899833 2 40 6.5817260488683225 2 41 10.067109110887767 2 42 8.19441655885634 2 43 7.839576978226045 2 44 -1.6285710792281165 2 45 7.734950168042634 2 46 8.332265707359404 2 47 3.9001301138160898 2 48 8.065903231390674 2 49 1.9083062543949971 2 50 8.319706779917734 2 51 9.70119276718125 2 52 9.621243840780341 2 53 9.962336825874676 2 54 10.021483777862308 2 55 9.934894202768955 2 56 12.046035526459974 3 1 3.3639977926447884 3 2 4.533917749560814 3 3 4.697229859383263 3 4 4.564716746215744 3 5 6.578619432018996 3 6 4.751989760231702 3 7 3.6945700550635436 3 8 3.887908965539779 3 9 3.9217755750015115 3 10 5.19228593479842 3 11 3.9699435803175547 3 12 5.094652742345957 3 13 5.744557293668642 3 14 4.703032312921337 3 15 1.276127854021773 3 16 6.289099508631043 3 17 8.03809124992911 3 18 8.600166520877906 3 19 7.351416176300351 3 20 6.775415982696472 3 21 7.855153261738266 3 22 8.562788735791088 3 23 7.524536341422884 3 24 8.650760923040934 3 25 7.3247103264272155 3 26 6.777123799962691 3 27 6.215196129497787 3 28 5.345542884433122 3 29 6.109656286556895 3 30 6.450973802170619 3 31 4.944524271166177 3 32 4.486927597105822 3 33 5.414334633740758 3 34 5.770632570740258 3 35 5.576507406585193 3 36 7.467047191608454 3 37 6.409543019385865 3 38 7.596230305043228 3 39 8.693043726476127 3 40 10.945310847507006 3 41 12.357523177819129 3 42 10.464978101681076 3 43 11.240030750205623 3 44 11.334559947430986 3 45 11.34357183565466 3 46 12.58467637100092 3 47 13.885949739454205 3 48 12.371064887658402 3 49 13.731902397333172 3 50 11.909610353372074 3 51 12.14872538204228 3 52 10.514040210604502 3 53 11.753034500293278 3 54 12.036231367198663 3 55 12.460702484107582 3 56 11.521361675070295 4 1 3.3384163972959513 4 2 4.558917749560813 4 3 4.702462417522803 4 4 4.523437676448303 4 5 6.151875245972484 4 6 4.587454876510776 4 7 3.7817793573891265 4 8 3.769885709725827 4 9 4.357822086629419 4 10 5.174844074333301 4 11 4.371687766364062 4 12 5.637675998159914 4 13 5.539324735529107 4 14 5.468148591991103 4 15 5.985430179603171 4 16 7.836192531886855 4 17 9.19855636620818 4 18 9.315864195296506 4 19 8.279904548393379 4 20 8.058555517580196 4 21 7.868525354761523 4 22 8.440695712535273 4 23 8.303024713515914 4 24 8.367621388157211 4 25 7.928780093869079 4 26 5.834681939497575 4 27 5.56577752484662 4 28 6.310077768154052 4 29 7.27419117027782 4 30 6.231206360310157 4 31 5.934059154887105 4 32 5.879369457570942 4 33 6.203869517461683 4 34 5.934586059112352 4 35 5.498019034492167 4 36 6.078093703236359 4 37 6.894426740316099 4 38 6.387509374810669 4 39 6.811066982290079 4 40 1.473799219600032 4 41 11.736011549912153 4 42 11.036489729588048 4 43 11.44933307578702 4 44 12.503745993942612 4 45 11.22031602170117 4 46 13.234094975652088 4 47 13.920252065035605 4 48 13.92687884114677 4 49 13.190623327565731 4 50 13.752633609186027 4 51 12.323143986693445 4 52 12.691365791999853 4 53 12.112336825874674 4 54 11.963556948594015 4 55 12.464772251549444 4 56 11.265547721581925 5 1 3.6574640163435745 5 2 4.819632035275099 5 3 4.865557655618041 5 4 5.091294819305446 5 5 7.125684769782012 5 6 5.064835828891724 5 7 4.055588881198652 5 8 4.009171424011541 5 9 4.560203039010368 5 10 5.30579645528568 5 11 4.8990687187450135 5 12 6.116247426731341 5 13 6.504800926005295 5 14 5.549100972943485 5 15 7.0806682748412655 5 16 8.161192531886858 5 17 9.422365890017709 5 18 9.93848324291556 5 19 8.804904548393377 5 20 8.404984089008764 5 21 8.391144402380576 5 22 9.238314760154324 5 23 8.382786618277816 5 24 8.759288054823877 5 25 8.222827712916697 5 26 7.438253368069002 5 27 7.064587048656147 5 28 6.746982530058808 5 29 6.939667360754012 5 30 7.73834921745301 5 31 4.91739248822044 5 32 6.301988505189993 5 33 6.934821898414064 5 34 6.036967011493299 5 35 5.242066653539787 5 36 7.295950846093508 5 37 7.3491886450780015 5 38 8.593461755763052 5 39 9.35749555371865 5 40 10.970227791028602 5 41 13.739582978483586 5 42 12.773394491492809 5 43 12.06838069483464 5 44 12.578745993942615 5 45 12.58222078360593 5 46 14.306714023271137 5 47 15.285728255511792 5 48 13.888783603051532 5 49 16.377528089470488 5 50 14.859776466328881 5 51 13.894572558122015 5 52 14.078270553904613 5 53 14.724241587779435 5 54 14.886175996213066 5 55 14.954057965835158 5 56 13.86078581682002 6 1 3.038416397295954 6 2 4.241234822731549 6 3 4.676242905327676 6 4 4.472827920350738 6 5 6.406753294752974 6 6 4.612454876510775 6 7 3.629340332998879 6 8 3.550983270701437 6 9 3.4736757451660054 6 10 5.302892854821103 6 11 4.678395083437238 6 12 5.407188193281861 6 13 6.1313979062608155 6 14 5.948636396869155 6 15 6.94884481374951 6 16 7.441680336764907 6 17 8.94733685401306 6 18 9.415254439198947 6 19 7.548806987417766 6 20 6.894531127336293 6 21 6.245964379151772 6 22 8.117524980827959 6 23 7.526805201320787 6 24 7.873109193035262 6 25 6.286706923137366 6 26 5.716999012668307 6 27 5.443216549236869 6 28 4.338736304739413 6 29 4.59065458491197 6 30 6.088523433480891 6 31 4.840156715862715 6 32 4.425101164888016 6 33 4.773381712583635 6 34 4.316293376185524 6 35 5.25716537595558 6 36 5.77504492274856 6 37 6.212719423242923 6 38 6.980802057737499 6 39 7.395823079851056 6 40 2.996360195209789 6 41 11.969548135278007 6 42 10.852953144222198 6 43 11.181040392860194 6 44 10.956794774430417 6 45 11.00324285096946 6 46 12.844460829310623 6 47 13.705008162596577 6 48 12.846391036268724 6 49 14.200989181224266 6 50 13.539218975039688 6 51 12.701192767181247 6 52 12.08465847492668 6 53 12.840385606362478 6 54 14.070264265667188 6 55 11.64221127593969 6 56 12.387498941094123 7 1 3.4331532394012143 7 2 4.428654591666074 7 3 4.7853571543649025 7 4 4.658963992237773 7 5 6.470296298604069 7 6 4.69140224493183 7 7 3.6370425152838664 7 8 3.8212014991995105 7 9 4.344664191892576 7 10 4.898528284859616 7 11 4.683529871627222 7 12 6.187675998159911 7 13 6.419587893423845 7 14 5.852359118306893 7 15 7.169640705918958 7 16 8.555929373992118 7 17 9.18803005041871 7 18 10.338232616349138 7 19 9.094378232603905 7 20 8.175660780738092 7 21 8.396156933708895 7 22 8.111748344114218 7 23 7.714866818779068 7 24 9.013674019736158 7 25 7.919569567553289 7 26 6.693892465813359 7 27 6.277619630109779 7 28 6.616656715522467 7 29 6.907085907119924 7 30 6.775943202415416 7 31 5.426164418045001 7 32 5.807001036518312 7 33 6.448606359566945 7 34 5.0319544801649805 7 35 6.254597981860584 7 36 7.889935808499519 7 37 7.812847792947679 7 38 8.238825164284357 7 39 8.73343540334271 7 40 7.372483430126348 7 41 12.28469576043847 7 42 11.56412130853542 7 43 13.291438338944918 7 44 12.55243020446893 7 45 12.2676844427538 7 46 14.390673923020504 7 47 14.042620486088232 7 48 14.442668314830986 7 49 13.905097011776256 7 50 14.081580977607082 7 51 14.454722934061863 7 52 13.65847105515775 7 53 14.357073667979936 7 54 14.943820106488754 7 55 13.266088041023128 7 56 14.002389826845082 8 1 3.196311134138057 8 2 4.560233539034499 8 3 4.864304522785957 8 4 4.422121886974615 8 5 6.391348930183014 8 6 4.401928560721302 8 7 3.715989883704914 8 8 3.3738330781468804 8 9 4.370979981366261 8 10 5.293265126964883 8 11 4.709845661100907 8 12 6.161360208686226 8 13 6.524851051318578 8 14 5.24709596041216 8 15 6.880167021708431 8 16 7.47698200557107 8 17 8.424872155681868 8 18 9.022443142664926 8 19 7.647009811551275 8 20 7.280923938632824 8 21 7.396156933708895 8 22 8.190695712535273 8 23 7.056972081936966 8 24 7.72420033552563 8 25 7.182727462290131 8 26 6.088629307918627 8 27 7.146040682741361 8 28 6.590340926048789 8 29 7.012349065014664 8 30 7.039101097152258 8 31 5.452480207518686 8 32 5.412264194413044 8 33 5.63281688588274 8 34 5.13721763805972 8 35 5.7282821923869065 8 36 6.468883176920574 8 37 5.970742529789781 8 38 5.370404111652775 8 39 6.575540666500608 8 40 2.846167640652663 8 41 10.363643128859525 8 42 10.169384466430152 8 43 9.712490970523863 8 44 10.526114414995245 8 45 9.504526548016958 8 46 13.180147607231032 8 47 12.542620486088232 8 48 13.179510420094143 8 49 11.536675959144674 8 50 12.31842308287024 8 51 11.559986091956603 8 52 10.974260528841956 8 53 11.98865261534836 8 54 11.496451685436117 8 55 12.450298567338915 8 56 9.844495090002976 9 1 3.3204676793472387 9 2 4.79032800597107 9 3 4.653103443163829 9 4 4.52536075337138 9 5 6.550593194690435 9 6 4.586813850869746 9 7 3.659984485594258 9 8 3.666680581520694 9 9 4.338591317398652 9 10 5.296638946128169 9 11 4.613354433030729 9 12 5.700496510980422 9 13 6.352786273990645 9 14 5.578405002247514 9 15 7.177737871910864 9 16 7.917602788297113 9 17 9.103684571336387 9 18 9.211377015809326 9 19 8.048494291983122 9 20 7.513042697067377 9 21 7.67685868809486 9 22 8.395823917663478 9 23 8.272896508387706 9 24 8.45159574713157 9 25 7.808908298997281 9 26 6.8814768112924405 9 27 6.95103393510303 9 28 6.177385460461743 9 29 6.992780913867566 9 30 7.58633456543836 9 31 5.955854026681973 9 32 5.946677149878635 9 33 5.4256643892565535 9 34 5.199970674496964 9 35 6.340967752440882 9 36 5.883862934005595 9 37 6.918785714675067 9 38 7.327893990195285 9 39 8.068118264341365 9 40 4.0270043478051605 9 41 11.97767821657882 9 42 12.742258960357283 9 43 11.500615127069072 9 44 11.87361778881441 9 45 11.85877756016271 9 46 13.900120616677729 9 47 15.45422642400996 9 48 14.960212174480109 9 49 15.20536691730932 9 50 14.61801822457064 9 51 15.02827219182165 9 52 14.175340150974211 9 53 13.552080415618267 9 54 14.748813358850423 9 55 14.329515841293034 9 56 13.829650285684487 10 1 3.477440787539855 10 2 4.8753811641949625 10 3 5.017706319961825 10 4 4.887462066692201 10 5 6.699436221582239 10 6 4.807576827730287 10 7 3.9220232598281513 10 8 4.014397904847776 10 9 4.034651354922104 10 10 4.863868464577202 10 11 4.87351703465675 10 12 5.846212583525762 10 13 6.399690589187642 10 14 5.485221762722816 10 15 7.0464057893592695 10 16 7.661192531886858 10 17 8.435141732061844 10 18 9.073791024564798 10 19 8.426855767905575 10 20 6.723799420019219 10 21 8.294744866956648 10 22 9.019964005218199 10 23 7.673146664735423 10 24 8.482865290596237 10 25 7.140365459722737 10 26 7.497486817546349 10 27 6.540777524846622 10 28 6.0948338657150245 10 29 6.980898487350995 10 30 6.478767335919908 10 31 4.206010374399302 10 32 4.571442628302652 10 33 4.748991468681197 10 34 4.7309275225269865 10 35 5.013262936931191 10 36 6.067727849577825 10 37 6.79808527690146 10 38 6.639338643103358 10 39 7.737286494485204 10 40 10.728067512282959 10 41 12.091499354790205 10 42 11.657831193002686 10 43 11.44933307578702 10 44 12.225087457357251 10 45 11.198364802188973 10 46 12.698119365895987 10 47 13.90013011381609 10 48 12.407366646024823 10 49 14.225379425126704 10 50 13.270926292112854 10 51 13.384119596449544 10 52 13.133438962731564 10 53 12.523312435630775 10 54 13.777581338837916 10 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1.2695914313526337 114 55 2.7742550101701333 114 56 5.231064962961234 115 1 2.423031781911341 115 2 3.6108408264838943 115 3 3.8966931867535664 115 4 3.833053061063687 115 5 5.75572139981864 115 6 3.843224107280008 115 7 3.0317793573891265 115 8 3.025654940495059 115 9 3.3001297789371122 115 10 4.040228689717914 115 11 2.8697646894409843 115 12 -0.12001630953239584 115 13 0.8143247355291052 115 14 0.32199474583725873 115 15 -3.4761082819352893 115 16 4.084269454963778 115 17 5.321633289285103 115 18 4.4293257337580485 115 19 3.574135317624144 115 20 3.8335555175801943 115 21 5.651217662453831 115 22 6.190695712535273 115 23 5.862640098131298 115 24 6.861852157387979 115 25 6.026857016945996 115 26 5.625066554882185 115 27 4.0023159863850815 115 28 4.523539306615589 115 29 4.633806554893209 115 30 5.150437129540922 115 31 3.622520693348644 115 32 3.6389848421863284 115 33 3.130792594384765 115 34 3.7384322129585037 115 35 3.4691728806460134 115 36 6.230016780159442 115 37 5.05981135570071 115 38 5.097124759426059 115 39 7.3629900592131605 115 40 9.514183834984646 115 41 9.080242319142922 115 42 9.357643575741896 115 43 6.141640768094714 115 44 -2.2674078522112318 115 45 8.82031602170117 115 46 3.9898642064213163 115 47 -7.712440242656706 115 48 0.9345711488390798 115 49 -2.0382228262804247 115 50 3.1564797630321806 115 51 5.400067063616522 115 52 5.93175040738447 115 53 5.577721441259293 115 54 6.184710794747861 115 55 4.970541482318673 115 56 8.304009260043465 116 1 3.009844968724522 116 2 4.619632035275103 116 3 4.770319560379946 116 4 4.505580533591157 116 5 6.116160960258199 116 6 4.612454876510775 116 7 3.3032079288176988 116 8 3.6234571382972547 116 9 3.793536372343702 116 10 5.139129788619016 116 11 4.203830623506924 116 12 3.3305331410170567 116 13 4.757181878386248 116 14 4.825291449133964 116 15 -3.1717126775396878 116 16 2.7754782461725718 116 17 5.812842080493894 116 18 4.219435623867938 116 19 5.362047405536234 116 20 3.590698374723054 116 21 5.572096783332952 116 22 5.704981426820986 116 23 5.7923104278016275 116 24 5.721192816728639 116 25 6.260922951011935 116 26 6.438253368069002 116 27 6.426491810560908 116 28 5.65650633958262 116 29 5.734905455992109 116 30 5.952634931738725 116 31 4.198344869172818 116 32 4.797226600428086 116 33 5.039583803175972 116 34 5.556014630540918 116 35 3.5658761773493097 116 36 5.567379417522073 116 37 5.515855311744666 116 38 6.598223660524958 116 39 7.186066982290079 116 40 9.298799219600031 116 41 8.825297264197868 116 42 6.340061158159479 116 43 0.7636187900727336 116 44 0.2216031367997573 116 45 5.806030307415455 116 46 0.6876664042235134 116 47 -5.076176506392969 116 48 1.31259312686106 116 49 -4.441519529577128 116 50 1.4026336091860259 116 51 4.623143986693446 116 52 4.102080077714138 116 53 4.70519396873182 116 54 4.538556948594014 116 55 3.9683436801208707 116 56 5.322690578724782 117 1 1.6747800336595873 117 2 3.215735931378994 117 3 3.3092805993409797 117 4 3.2211649491755736 117 5 5.37460251869976 117 6 3.430636694692595 117 7 2.3045066301164 117 8 2.4312493460894586 117 9 2.6532766320839656 117 10 4.061207710696934 117 11 -1.1896758699995686 117 12 -11.31232400184009 117 13 -11.594766173561805 117 14 -2.604578680736168 117 15 -6.469115274942283 117 16 -7.884262013567689 117 17 1.0349200025718197 117 18 -3.6545903501580383 117 19 0.4412681847570141 117 20 -1.2573535733288956 117 21 4.116252627488798 117 22 5.281604803444367 117 23 4.250751986243184 117 24 3.138075933611759 117 25 3.87650736659635 117 26 5.509681939497572 117 27 5.631686615755715 117 28 4.803259586335869 117 29 5.7946457157323685 117 30 5.926660905764702 117 31 4.402240973068924 117 32 2.4816421848436683 117 33 4.872051335643505 117 34 3.7664042409305303 117 35 3.032109943583073 117 36 4.6985482486909085 117 37 5.308063103952463 117 38 5.9852366475379455 117 39 5.740612436835534 117 40 7.035162855963666 117 41 5.992829731730339 117 42 3.0114897295880496 117 43 -2.6415760151220695 117 44 -3.6485267333301117 117 45 4.638497839882987 117 46 -0.7409050243479136 117 47 -3.404747934964398 117 48 1.8086970229649566 117 49 -5.748013036070635 117 50 0.04808815464057403 117 51 2.7776894412388984 117 52 2.3618203374544002 117 53 2.0077913713292226 117 54 1.7476478576849246 117 55 0.648863160640353 117 56 2.9928204488546513 118 1 1.564732186769639 118 2 2.9023388021923964 118 3 2.6800939964701698 118 4 2.974753465921985 118 5 3.917664719656699 118 6 2.980875929142357 118 7 2.1107267258101814 118 8 2.2159383413047706 118 9 2.476243139261001 118 10 3.635370390122773 118 11 -0.2638385494254081 118 12 -7.075481896576932 118 13 -7.764622632891946 118 14 -8.30553561853521 118 15 -15.304043504607357 118 16 -7.286175889165772 118 17 -2.101443633791817 118 18 -7.188083173124543 118 19 -0.958253346343465 118 20 -1.6138129034724358 118 21 4.86984114423521 118 22 5.032800975693171 118 23 4.1096036608843285 118 24 5.145252967104582 118 25 4.709043251763816 118 26 4.167576676339674 118 27 5.224988051162409 118 28 4.906130399732994 118 29 6.091296433435719 118 30 5.381206360310156 118 31 3.663006523308159 118 32 3.043843141781469 118 33 3.7117642543037874 118 34 4.795112374901823 118 35 3.6756506134395366 118 36 6.126777913762677 118 37 6.3654793718950415 118 38 6.975667269547515 118 39 8.101856455974293 118 40 11.056693956442135 118 41 9.679432602543738 118 42 9.61675288748279 118 43 -1.7085616610550858 118 44 -1.44756979553107 118 45 7.320316021701171 118 46 -0.6882734454005437 118 47 -1.0100110928591342 118 48 -1.8994369483269118 118 49 2.6156233275657286 118 50 2.318423082870236 118 51 7.981038723535551 118 52 7.8163657919998535 118 53 6.488652615348361 118 54 7.654346422278223 118 55 7.266088041023128 118 56 9.42344245842403 119 1 2.1124904713700303 119 2 3.533917749560814 119 3 3.5163513064116856 119 4 3.4484376764483002 119 5 5.638912283009525 119 6 3.5383808024366985 119 7 2.309557135166905 119 8 1.6467375615776803 119 9 2.663377642184976 119 10 4.3878070372962625 119 11 1.3540951737714693 119 12 -10.145657335173421 119 13 -2.6671567459523757 119 14 -4.646666222823708 119 15 -9.662717968544978 119 16 -7.709177838483512 119 17 -0.02736955971774435 119 18 -3.498024693592381 119 19 -0.17657693308810352 119 20 -1.7775555935309164 119 21 5.121303132539303 119 22 6.153658675498235 119 23 5.3298765653677656 119 24 4.537065832601655 119 25 5.677854167943153 119 26 6.04671897653461 119 27 5.800036784105881 119 28 4.1147073977836754 119 29 5.5825245036111575 119 30 6.751576730680529 119 31 4.639614710442661 119 32 5.883999087200568 119 33 5.368684332276501 119 34 5.58458605911235 119 35 4.289685701158831 119 36 5.838278888421545 119 37 6.277760073649432 119 38 5.860657522958817 119 39 7.230511426734523 119 40 9.639539960340773 119 41 6.125826364726969 119 42 3.9559341740324925 119 43 -0.06918544273149863 119 44 -3.550883635687015 119 45 6.394390095775243 119 46 -2.0742383576812458 119 47 -2.386229416445879 119 48 -6.299047084779154 119 49 -3.643635931693531 119 50 -0.6640330574806406 119 51 4.378699542249002 119 52 4.112662088296151 119 53 3.962336825874676 119 54 3.4867050967421633 119 55 4.961994473771664 119 56 6.006288462322665 120 1 1.6384163972959556 120 2 2.6539177495608115 120 3 3.147462417522803 120 4 2.848437676448299 120 5 4.6018752459724865 120 6 2.172454876510777 120 7 1.651779357389124 120 8 1.7948857097258255 120 9 2.147822086629418 120 10 2.9448440743333038 120 11 -2.0933122336359347 120 12 -8.332324001840089 120 13 -8.385675264470894 120 14 -9.351851408008894 120 15 -2.6745698203968296 120 16 -7.8188074681131425 120 17 -0.8214436337918158 120 18 -7.969135804703491 120 19 -2.135095451606624 120 20 -1.4664444824198064 120 21 3.143525354761522 120 22 5.35069571253527 120 23 4.9180247135159085 120 24 3.732621388157213 120 25 4.483780093869079 120 26 5.4496819394975695 120 27 5.30077752484662 120 28 5.365077768154052 120 29 5.369191170277819 120 30 5.1012063603101545 120 31 3.2840591548871068 120 32 3.294369457570941 120 33 3.833869517461686 120 34 3.5845860591123504 120 35 2.6230190344921667 120 36 3.5330937032363643 120 37 3.1444267403160993 120 38 3.8325093748106696 120 39 5.106066982290081 120 40 5.058799219600033 120 41 -8.128988450087844 120 42 -4.968510270411951 120 43 -11.51066692421298 120 44 -16.401254006057385 120 45 -4.439683978298831 120 46 -10.220905024347914 120 47 -8.064747934964398 120 48 -6.573121158853226 120 49 -15.304376672434271 120 50 -4.637366390813973 120 51 -0.17685601330655487 120 52 -0.6636342080001469 120 53 -0.43766317412532274 120 54 -0.30144305140598604 120 55 0.3797722515494435 120 56 3.865547721581926 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 578.0 5 11.0 6 87.0 7 100.0 8 112.0 9 166.0 10 125.0 11 87.0 12 73.0 13 46.0 14 76.0 15 112.0 16 99.0 17 159.0 18 218.0 19 262.0 20 338.0 21 468.0 22 556.0 23 612.0 24 705.0 25 829.0 26 858.0 27 897.0 28 963.0 29 1046.0 30 1087.0 31 1319.0 32 1486.0 33 1531.0 34 1745.0 35 1858.0 36 2165.0 37 2195.0 38 2373.0 39 2468.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 0.0 0.0 100.0 0.0 3 0.0 0.0 0.0 100.0 4 0.0 0.0 0.0 100.0 5 0.0 100.0 0.0 0.0 6 0.0 0.0 100.0 0.0 7 99.42466738583244 0.4207119741100324 0.06112909025530385 0.09349154980222942 8 99.4066882416397 0.2840704782452355 0.15102481121898598 0.15821646889608054 9 97.75971807688158 0.8378582473300011 0.9061814520478982 0.49624222374051563 10 53.61380798274003 23.746853649766273 14.18554476806904 8.453793599424667 11 32.491909385113274 24.890327220424307 17.335490830636463 25.282272563825963 12 31.63250629270047 21.877022653721685 21.743976986695436 24.746494066882416 13 23.29737504494786 14.58108594030924 21.94534340165408 40.176195613088815 14 20.89895720963682 29.424667385832436 26.0913340524991 23.585041352031645 15 31.963156944193607 18.6346396965866 22.279212569983745 27.122990789236052 16 26.486039147956248 27.921704087507198 23.513960852043752 22.078295912492806 17 27.815736478711163 23.26668584579977 29.714470655926352 19.203107019562715 18 24.361740381157855 20.21934555915138 25.742538655160015 29.67637540453075 19 27.747203338009424 21.20427322758174 21.707852235531096 29.34067119887774 20 27.618121578795733 23.99884759435321 28.504033419763758 19.878997407087297 21 26.45091693635383 20.44947860481841 25.008989572096368 28.09061488673139 22 22.41999280834232 19.74829198130169 32.58899676375405 25.24271844660194 23 21.765551959726718 23.585041352031645 30.834232290542968 23.81517439769867 24 22.345283900895392 24.50285878672372 23.34496026466252 29.806897047718365 25 23.663106412054518 23.033768475563708 28.108030352069623 25.195094760312152 26 33.18703457207612 23.901859912940246 20.333129474403712 22.57797604057992 27 22.888185350410133 28.730752626277162 21.535472729889193 26.845589293423515 28 26.159654800431497 29.87774181948939 20.34160373966199 23.620999640417118 29 31.393171012822357 26.656821783604666 20.82192767612736 21.12807952744561 30 28.379739549607883 23.31462695157925 26.58824375854378 21.717389740269084 31 31.44758137025715 25.761553677396154 22.19025355151951 20.60061140082719 32 32.26757777378169 26.430498111850387 22.611041179643948 18.69088293472397 33 31.52040889784753 24.544669210280038 21.899071341156144 22.03585055071629 34 23.076368876080693 23.490634005763688 33.8328530259366 19.600144092219022 35 21.986173124009795 25.89658648998992 34.60319746507273 17.51404292092755 36 26.05317120552578 25.869698168867146 26.35176457891139 21.725366046695687 37 27.322148617511523 23.92713133640553 29.03585829493088 19.714861751152075 38 29.702649125968644 24.99909893674536 25.539736889529646 19.758515047756354 39 27.96658986175115 24.931595622119815 22.062211981566822 25.03960253456221 40 22.919385239959404 19.26199797013194 25.496592721473103 32.32202406843555 41 19.078063133422933 16.913073486141887 22.99030113698282 41.01856224345236 42 21.72314347512617 27.638788752703675 24.64671953857246 25.991348233597694 43 19.010707718931393 23.650719255867614 28.027544435230915 29.31102858997008 44 20.550962198071993 26.25194064339098 27.33509044300827 25.862006715528757 45 25.754954954954957 23.282882882882884 28.54774774774775 22.414414414414413 46 19.93508835196538 27.962495492246664 25.49224666426253 26.610169491525426 47 18.760614272809395 24.37940379403794 24.238482384823847 32.62149954832881 48 25.492030367358687 23.54189903932645 26.366351239520743 24.599719353794118 49 21.20840693608277 25.253253541944552 24.05998774288907 29.4783517790836 50 22.271450183704346 26.644333981701607 25.57812837691809 25.506087457675957 51 21.070668446304246 27.484578478409873 26.19313877565744 25.25161429962844 52 20.738206697875405 30.039611091105513 27.562117392870007 21.66006481814908 53 20.59491617090319 29.248242293131423 27.57526590950063 22.581575626464755 54 19.984136712694234 24.764754659840644 30.598118037278727 24.652990590186395 55 19.39857214970794 23.99581740823538 29.800966322924932 26.804644119131755 56 20.503117454139186 23.898079071611345 30.886221933902764 24.712581540346704 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 1.0 9 2.0 10 20.0 11 38.0 12 66.5 13 106.5 14 118.0 15 101.5 16 85.0 17 83.5 18 82.0 19 83.0 20 84.0 21 84.0 22 83.5 23 83.0 24 80.5 25 78.0 26 92.0 27 106.0 28 124.5 29 168.0 30 193.0 31 201.5 32 210.0 33 248.0 34 286.0 35 377.0 36 468.0 37 454.5 38 446.0 39 451.0 40 505.0 41 559.0 42 672.0 43 785.0 44 977.5 45 1170.0 46 1571.0 47 2072.5 48 2173.0 49 1965.0 50 1757.0 51 1677.0 52 1597.0 53 1618.0 54 1718.5 55 1798.0 56 1841.0 57 1884.0 58 1870.0 59 1856.0 60 1813.5 61 1771.0 62 1706.0 63 1426.0 64 1211.0 65 1062.0 66 913.0 67 770.0 68 627.0 69 545.0 70 463.0 71 397.5 72 297.5 73 263.0 74 206.0 75 149.0 76 109.5 77 70.0 78 58.0 79 53.5 80 61.0 81 39.5 82 18.0 83 12.0 84 6.0 85 3.5 86 1.0 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0035958288385472856 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.4494786048184107 16 0.06472491909385113 17 0.007191657677094571 18 0.0 19 0.032362459546925564 20 0.15102481121898598 21 0.0 22 0.0 23 0.0 24 0.0035958288385472856 25 0.010787486515641856 26 0.04674577490111471 27 0.05034160373966199 28 0.0 29 0.16540812657317513 30 0.043149946062567425 31 0.017979144192736426 32 0.017979144192736426 33 0.10068320747932398 34 0.17979144192736426 35 0.13664149586479685 36 0.04674577490111471 37 0.1222581805106077 38 0.23372887450557353 39 0.1222581805106077 40 0.7982740021574973 41 1.0104279036317871 42 0.25170801869830994 43 0.2624955052139518 44 0.4063286587558433 45 0.2157497303128371 46 0.2876663070837828 47 0.48543689320388345 48 0.06112909025530385 49 0.25530384753685725 50 0.1725997842502697 51 0.3200287666307084 52 0.1438331535418914 53 0.2696871628910464 54 0.2624955052139518 55 0.2732829917295937 56 0.22653721682847897 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 27810.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.23840345199568 #Duplication Level Percentage of deduplicated Percentage of total 1 89.32029795158287 68.9895720963682 2 6.741154562383612 10.413520316432939 3 1.675977653631285 3.8834951456310676 4 0.8147113594040969 2.5170801869830997 5 0.4422718808193668 1.7080186983099606 6 0.25139664804469275 1.1650485436893203 7 0.12569832402234637 0.6796116504854369 8 0.10242085661080075 0.6328658755843222 9 0.06052141527001862 0.4207119741100324 >10 0.4236499068901303 5.634663790003596 >50 0.027932960893854747 1.4886731391585761 >100 0.013966480446927373 2.4667385832434374 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTCCATGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT 310 1.1147069399496585 No Hit CTCCATGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC 245 0.8809780654440849 No Hit CTCCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 131 0.4710535778496943 No Hit CTCCATGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC 76 0.2732829917295937 No Hit CTCCATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 72 0.2588996763754045 No Hit CTCCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 70 0.25170801869830994 No Hit CTCCATGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT 69 0.24811218985976266 No Hit CTCCATGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT 67 0.2409205321826681 No Hit CTCCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 60 0.2157497303128371 No Hit CTCCATGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA 49 0.17619561308881698 No Hit CTCCATGGGAGAAATAAACGCTTGAATACATCCAGCCTTGCTTATTTCAAATATTT 43 0.15462064005753326 No Hit CTCCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 40 0.1438331535418914 No Hit CTCCATGGGGCTCTCCATGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAA 40 0.1438331535418914 No Hit CTCCATGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCTC 35 0.12585400934915497 No Hit CTCCATGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCC 34 0.1222581805106077 No Hit CTCCATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 33 0.11866235167206042 No Hit CTCCATGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT 30 0.10787486515641855 No Hit CTCCATGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC 30 0.10787486515641855 No Hit CTCCATGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA 28 0.10068320747932398 No Hit CTCCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC 28 0.10068320747932398 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.01438331535418914 0.0 0.0 0.0 0.0 11 0.043149946062567425 0.0 0.0 0.0 0.0 12 0.04674577490111471 0.0 0.0 0.0 0.0 13 0.04674577490111471 0.0 0.0 0.0 0.0 14 0.06832074793239842 0.0 0.0 0.0 0.0 15 0.06832074793239842 0.0 0.0 0.0 0.0 16 0.0719165767709457 0.0 0.0 0.0 0.0 17 0.07910823444804027 0.0 0.0 0.0 0.0 18 0.07910823444804027 0.0 0.0 0.0 0.0 19 0.10068320747932398 0.0 0.0 0.0 0.0 20 0.10427903631787126 0.0 0.0 0.0 0.0 21 0.10787486515641856 0.0 0.0 0.0 0.0 22 0.11506652283351312 0.0 0.0 0.0 0.0 23 0.12225818051060769 0.0 0.0 0.0 0.0 24 0.12944983818770225 0.0 0.0 0.0 0.0 25 0.12944983818770225 0.0 0.0 0.0 0.0 26 0.13664149586479685 0.0 0.0 0.0 0.0 27 0.1474289823804387 0.0 0.0 0.0 0.0 28 0.1725997842502697 0.0 0.0 0.0 0.0 29 0.1725997842502697 0.0 0.0 0.003595828838547285 0.0 30 0.1725997842502697 0.0 0.0 0.003595828838547285 0.0 31 0.17979144192736426 0.0 0.0 0.003595828838547285 0.0 32 0.17979144192736426 0.0 0.0 0.003595828838547285 0.0 33 0.17979144192736426 0.0 0.0 0.003595828838547285 0.0 34 0.17979144192736426 0.0 0.0 0.003595828838547285 0.0 35 0.17979144192736426 0.0 0.0 0.003595828838547285 0.0 36 0.17979144192736426 0.0 0.0 0.003595828838547285 0.0 37 0.18338727076591155 0.0 0.0 0.003595828838547285 0.0 38 0.18338727076591155 0.0 0.0 0.003595828838547285 0.0 39 0.1905789284430061 0.0 0.0 0.003595828838547285 0.0 40 0.1941747572815534 0.0 0.0 0.003595828838547285 0.0 41 0.19777058612010068 0.0 0.0 0.003595828838547285 0.0 42 0.20496224379719524 0.0 0.0 0.003595828838547285 0.0 43 0.22294138798993168 0.0 0.0 0.003595828838547285 0.0 44 0.22653721682847897 0.0 0.0 0.003595828838547285 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCCTCCG 15 0.008146357 50.304897 42 CCTATCC 15 0.00820511 50.213768 40 TCCTATC 15 0.00820511 50.213768 39 TTTCCTA 15 0.00820511 50.213768 37 GGCGCAT 15 0.00820511 50.213768 37 TTCCTAT 15 0.00820511 50.213768 38 GCGCATT 15 0.00820511 50.213768 38 CGCATTG 15 0.00820511 50.213768 39 CAATGGC 15 0.008323562 50.03249 15 TTCAATG 15 0.008323562 50.03249 13 CATCCTC 15 0.008323562 50.03249 15 AGAACGC 15 0.008323562 50.03249 48 ACTCATC 15 0.008323562 50.03249 50 GACGAGA 15 0.008323562 50.03249 14 TGAGGGT 15 0.008323562 50.03249 49 CAACATT 15 0.008383263 49.94234 29 CCTTGAC 15 0.008383263 49.94234 29 CTTGACC 15 0.008383263 49.94234 30 CGGTTCG 15 0.008383263 49.94234 30 CCGGTTC 15 0.008383263 49.94234 29 >>END_MODULE