FastQCFastQC Report
Fri 17 Jun 2016
SRR1525913_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525913_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3251
Sequences flagged as poor quality0
Sequence length40
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT110.3383574284835435No Hit
CTCCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC100.30759766225776686No Hit
CTCCATGGGTACCTGGTTGATCCTGCCCGTAGCATATGCT80.2460781298062135No Hit
CTCCATGGGGGGCTGGTGAGATGGCTCCGTGGGTAAGAGC80.2460781298062135No Hit
CTCCATGGGTACCTGGTTGATCCTGCCCGTAGCCTATGCT60.1845585973546601No Hit
CTCCATGGGTCGCAGAGTCGATTTTTTTTTTTTTTTTTTT50.15379883112888343No Hit
CTCCATGGGGGGGCTGGTGAGATGGCTCCGTGGGTAAGAG50.15379883112888343No Hit
CTCCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG50.15379883112888343No Hit
CTCCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCC40.12303906490310675No Hit
CTCCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG40.12303906490310675No Hit
CTCCATGGGGGGCTGGTGCGATGGCTCAGTGGGTAAGAGC40.12303906490310675No Hit
CTCCATGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT40.12303906490310675No Hit
CTCCATGGGATCCTGGGCCATTGGCCTCTCTGTGGCCGAC40.12303906490310675No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGGT251.618756E-434.05
ATGGGGG800.034.05
ATGGGGC200.00240132434.05
ATGGGGA301.0804797E-534.05
TGGGGGG551.2732926E-1134.06
CATGGGT251.618756E-434.04
CATGGGG1550.034.04
CTCCATG3300.032.9696961
TCCATGG3200.032.93752
CCATGGG3000.032.8666653
CATGGGA700.031.5714284
CATGGGC508.414645E-930.5999984
ATGGGAT353.1064832E-529.1428575
ATGGGCT250.007164519727.1999995
GGGGGGG455.088983E-626.4444437
Produced by FastQC (version 0.11.5)