##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1525913_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3251
Sequences flagged as poor quality	0
Sequence length	40
%GC	55
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.811750230698248	31.0	30.0	33.0	4.0	35.0
2	17.23408182097816	17.0	4.0	27.0	4.0	34.0
3	17.700707474623194	18.0	4.0	29.0	4.0	35.0
4	19.18732697631498	22.0	4.0	31.0	4.0	35.0
5	12.6198092894494	9.0	4.0	19.0	4.0	29.0
6	16.385112273146724	16.0	4.0	27.0	4.0	33.0
7	17.07812980621347	19.0	4.0	27.0	4.0	33.0
8	15.78775761304214	16.0	4.0	25.0	4.0	31.0
9	16.40479852353122	17.0	4.0	27.0	4.0	33.0
10	13.311596431867118	10.0	4.0	21.0	4.0	30.0
11	18.065825899723162	19.0	4.0	31.0	4.0	35.0
12	18.175023069824668	20.0	4.0	31.0	4.0	35.0
13	18.201476468778836	20.0	4.0	31.0	4.0	35.0
14	18.192556136573362	20.0	4.0	31.0	4.0	35.0
15	17.455244540141496	16.0	4.0	31.0	4.0	35.0
16	14.98892648415872	4.0	4.0	28.0	4.0	33.0
17	14.379883112888342	4.0	4.0	27.0	4.0	32.0
18	14.115041525684404	4.0	4.0	27.0	4.0	32.0
19	12.651184250999693	4.0	4.0	24.0	4.0	32.0
20	12.980928944940018	4.0	4.0	26.0	4.0	33.0
21	11.12119347892956	4.0	4.0	20.0	4.0	30.0
22	11.023992617656106	4.0	4.0	19.0	4.0	31.0
23	10.689326361119656	4.0	4.0	18.0	4.0	30.0
24	10.136573362042448	4.0	4.0	14.0	4.0	30.0
25	9.955398338972623	4.0	4.0	12.0	4.0	30.0
26	8.454014149492464	4.0	4.0	4.0	4.0	27.0
27	8.414949246385728	4.0	4.0	4.0	4.0	28.0
28	8.107659181790218	4.0	4.0	4.0	4.0	27.0
29	7.643801907105506	4.0	4.0	4.0	4.0	25.0
30	7.095662872962166	4.0	4.0	4.0	4.0	21.0
31	7.226084281759459	4.0	4.0	4.0	4.0	23.0
32	6.970470624423254	4.0	4.0	4.0	4.0	20.0
33	7.025838203629652	4.0	4.0	4.0	4.0	21.0
34	6.891418025223008	4.0	4.0	4.0	4.0	20.0
35	6.776684097200861	4.0	4.0	4.0	4.0	20.0
36	6.943402030144571	4.0	4.0	4.0	4.0	19.0
37	6.83881882497693	4.0	4.0	4.0	4.0	16.0
38	6.725315287603814	4.0	4.0	4.0	4.0	7.0
39	6.6810212242386955	4.0	4.0	4.0	4.0	4.0
40	6.634881574900031	4.0	4.0	4.0	4.0	4.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	4.344502801120452
1	2	11.576447245564893
1	3	-8.889966542172427
1	4	3.2375155617802704
1	5	11.514947089947087
1	6	7.270152505446628
1	7	10.170273109243695
1	8	4.181185807656398
1	9	10.347233893557423
1	10	9.694821817615935
1	11	7.287165421724243
1	12	6.938515406162466
1	13	-4.371942110177404
1	14	8.222222222222221
1	15	9.062819016495485
1	16	8.279964207905385
1	17	11.480777310924369
1	18	6.440238873327107
1	19	-0.26690787426081464
1	20	8.094798474945534
1	21	16.156189698101464
1	22	16.999470899470897
1	23	7.660955493308439
1	24	12.271619203236849
1	25	12.15683551198257
1	26	11.824463118580766
1	27	14.794172113289761
1	28	11.514476346093991
1	29	8.311297852474322
1	30	7.348202614379084
1	31	17.308523965141614
1	32	18.294078742608153
1	33	17.713873327108622
1	34	20.13141923436041
1	35	18.304427326486152
1	36	21.272751322751322
1	37	20.208609554933084
1	38	14.657753657018361
1	39	16.840518985371926
1	40	16.608492841581075
4	1	-14.788830532212883
4	2	-9.023552754435109
4	3	-7.023299875505762
4	4	-10.76248443821973
4	5	-1.8850529100529112
4	6	-6.996514161220039
4	7	-6.029726890756304
4	8	-3.485480859010268
4	9	-11.719432773109244
4	10	-6.0385115157173965
4	11	-8.512834578275758
4	12	-3.1948179271708685
4	13	-4.371942110177404
4	14	-7.311111111111112
4	15	-6.603847650171181
4	16	-6.053369125427948
4	17	-6.119222689075633
4	18	-3.959761126672891
4	19	-1.0669078742608153
4	20	-5.238534858387799
4	21	2.1561896981014623
4	22	3.4661375661375633
4	23	2.0609554933084375
4	24	3.0716192032368497
4	25	3.623502178649236
4	26	6.291129785247432
4	27	5.19417211328976
4	28	3.1144763460939924
4	29	4.977964519140988
4	30	4.61486928104575
4	31	5.375190631808278
4	32	5.76074540927482
4	33	6.913873327108622
4	34	5.464752567693745
4	35	3.3044273264861515
4	36	2.739417989417989
4	37	7.675276221599751
4	38	-0.5422463429816373
4	39	5.440518985371927
4	40	7.075159508247744
5	1	5.211169467787116
5	2	3.3097805788982235
5	3	-7.578855431061317
5	4	-8.87359554933084
5	5	3.2260582010581995
5	6	1.1145969498910695
5	7	-0.6963935574229723
5	8	-0.48548085901026816
5	9	-0.05276610644257573
5	10	-1.8162892934951742
5	11	-2.2906123560535363
5	12	-0.8614845938375346
5	13	-2.371942110177404
5	14	-1.9777777777777779
5	15	-0.3816254279489577
5	16	-3.4978135698723936
5	17	1.8807773109243673
5	18	1.8180166511048874
5	19	6.821981014628074
5	20	5.317020697167756
5	21	4.711745253657018
5	22	4.910582010582008
5	23	7.50539993775288
5	24	6.960508092125739
5	25	9.06794662309368
5	26	-0.708870214752567
5	27	0.9719498910675375
5	28	-0.6633014316837853
5	29	0.31129785247432196
5	30	1.6148692810457508
5	31	2.264079520697167
5	32	2.205189853719265
5	33	2.0249844382197324
5	34	2.464752567693745
5	35	2.1933162153750407
5	36	3.294973544973544
5	37	2.4530539993775298
5	38	4.679975879240584
5	39	1.5516300964830378
5	40	1.8529372860255222
6	1	8.044502801120451
6	2	6.476447245564891
6	3	9.810033457827572
6	4	1.7375155617802704
6	5	13.114947089947089
6	6	13.670152505446627
6	7	13.970273109243696
6	8	2.681185807656398
6	9	14.447233893557424
6	10	10.794821817615937
6	11	15.487165421724242
6	12	11.638515406162465
6	13	12.128057889822596
6	14	10.022222222222222
6	15	8.062819016495485
6	16	4.279964207905385
6	17	10.880777310924367
6	18	7.040238873327109
6	19	12.933092125739185
6	20	6.594798474945534
6	21	21.656189698101464
6	22	21.799470899470897
6	23	22.560955493308438
6	24	17.07161920323685
6	25	19.456835511982568
6	26	15.124463118580767
6	27	14.19417211328976
6	28	25.114476346093994
6	29	-2.688702147525678
6	30	-2.051797385620916
6	31	-2.2914760348583885
6	32	-1.9059212573918458
6	33	-2.0861266728913783
6	34	-1.8685807656395887
6	35	-1.6955726735138485
6	36	-1.9272486772486772
6	37	-1.9913904450669149
6	38	-1.5422463429816373
6	39	-1.5594810146280729
6	40	-1.5915071584189224
7	1	-10.705497198879549
7	2	-10.523552754435109
7	3	-9.439966542172428
7	4	-11.76248443821973
7	5	-6.635052910052911
7	6	-6.829847494553373
7	7	-7.029726890756304
7	8	-5.068814192343602
7	9	-7.552766106442576
7	10	-6.2051781823840635
7	11	-0.012834578275757735
7	12	1.1385154061624654
7	13	0.6280578898225961
7	14	1.2722222222222221
7	15	1.0628190164954852
7	16	-2.720035792094615
7	17	-2.3692226890756327
7	18	-2.209761126672891
7	19	-2.3169078742608153
7	20	-1.1552015250544656
7	21	-1.0938103018985377
7	22	-0.9505291005291028
7	23	-0.4390445066915625
7	24	1.8216192032368497
7	25	-5.04316448801743
7	26	-3.375536881419234
7	27	-2.80582788671024
7	28	-2.8855236539060076
7	29	-2.688702147525678
7	30	-2.051797385620916
7	31	-2.2914760348583885
7	32	-1.9059212573918458
7	33	-2.0861266728913783
7	34	-1.8685807656395887
7	35	-1.6955726735138485
7	36	-1.9272486772486772
7	37	-1.9913904450669149
7	38	-1.5422463429816373
7	39	-1.5594810146280729
7	40	-1.5915071584189224
9	1	-3.2054971988795486
9	2	-6.023552754435109
9	3	-5.314966542172428
9	4	-4.0124844382197296
9	5	-5.635052910052911
9	6	-3.4548474945533734
9	7	-0.9047268907563044
9	8	0.9311858076563979
9	9	-0.30276610644257573
9	10	0.5448218176159365
9	11	3.3621654217242423
9	12	1.1385154061624654
9	13	-0.12194211017740386
9	14	-3.477777777777778
9	15	2.187819016495485
9	16	6.029964207905385
9	17	5.005777310924367
9	18	3.040238873327109
9	19	2.4330921257391847
9	20	3.9697984749455344
9	21	4.656189698101462
9	22	3.9244708994708972
9	23	3.5609554933084375
9	24	5.19661920323685
9	25	0.33183551198257
9	26	-2.250536881419234
9	27	-1.1808278867102402
9	28	0.7394763460939924
9	29	-2.438702147525678
9	30	-1.0517973856209162
9	31	0.7085239651416115
9	32	0.09407874260815419
9	33	1.2888733271086217
9	34	-1.1185807656395887
9	35	0.6794273264861515
9	36	2.322751322751323
9	37	1.8836095549330851
9	38	2.3327536570183627
9	39	2.315518985371927
9	40	2.2834928415810776
10	1	4.669502801120451
10	2	-4.273552754435109
10	3	-1.189966542172428
10	4	-5.3874844382197296
10	5	-3.385052910052911
10	6	-0.45484749455337337
10	7	-1.0297268907563044
10	8	-1.818814192343602
10	9	-1.1777661064425757
10	10	-0.3301781823840635
10	11	-0.012834578275757735
10	12	-0.11148459383753462
10	13	1.3780578898225961
10	14	-0.22777777777777786
10	15	3.062819016495485
10	16	0.02996420790538501
10	17	-0.8692226890756327
10	18	1.6652388733271088
10	19	2.6830921257391847
10	20	1.5947984749455344
10	21	3.2811896981014623
10	22	2.1744708994708972
10	23	3.3109554933084375
10	24	1.8216192032368497
10	25	0.70683551198257
10	26	2.999463118580766
10	27	3.56917211328976
10	28	-0.8855236539060076
10	29	-0.43870214752567804
10	30	0.19820261437908382
10	31	-0.6664760348583885
10	32	-0.6559212573918458
10	33	-1.4611266728913783
10	34	-1.1185807656395887
10	35	0.42942732648615145
10	36	2.072751322751323
10	37	1.7586095549330851
10	38	2.2077536570183627
10	39	2.190518985371927
10	40	1.6584928415810776
11	1	-23.45549719887955
11	2	-14.023552754435109
11	3	-14.689966542172428
11	4	-15.76248443821973
11	5	-8.885052910052911
11	6	-12.329847494553373
11	7	-13.029726890756304
11	8	-11.818814192343602
11	9	-12.052766106442576
11	10	-8.705178182384063
11	11	-12.512834578275758
11	12	-12.861484593837535
11	13	-13.371942110177404
11	14	-12.977777777777778
11	15	-12.937180983504515
11	16	-9.720035792094615
11	17	-9.119222689075633
11	18	-8.959761126672891
11	19	-7.066907874260815
11	20	-7.905201525054466
11	21	-5.843810301898538
11	22	-6.200529100529103
11	23	-5.9390445066915625
11	24	-4.92838079676315
11	25	-5.04316448801743
11	26	-3.375536881419234
11	27	-2.80582788671024
11	28	-2.8855236539060076
11	29	-2.688702147525678
11	30	-2.051797385620916
11	31	-2.2914760348583885
11	32	-1.9059212573918458
11	33	-2.0861266728913783
11	34	-1.8685807656395887
11	35	-1.6955726735138485
11	36	-1.9272486772486772
11	37	-1.9913904450669149
11	38	-1.5422463429816373
11	39	-1.5594810146280729
11	40	-1.5915071584189224
12	1	-13.055497198879548
12	2	-11.82355275443511
12	3	-10.489966542172429
12	4	-8.962484438219729
12	5	-6.885052910052911
12	6	-7.329847494553373
12	7	-7.229726890756304
12	8	-7.018814192343601
12	9	-7.252766106442575
12	10	-2.305178182384063
12	11	-2.1128345782757574
12	12	-1.261484593837535
12	13	-1.5719421101774032
12	14	-0.9777777777777779
12	15	-0.5371809835045163
12	16	-5.120035792094615
12	17	-3.519222689075633
12	18	-3.3597611266728915
12	19	-0.666907874260815
12	20	-1.305201525054466
12	21	-0.4438103018985373
12	22	-6.200529100529103
12	23	-5.9390445066915625
12	24	-4.92838079676315
12	25	-5.04316448801743
12	26	-3.375536881419234
12	27	-2.80582788671024
12	28	-2.8855236539060076
12	29	-2.688702147525678
12	30	-2.051797385620916
12	31	-2.2914760348583885
12	32	-1.9059212573918458
12	33	-2.0861266728913783
12	34	-1.8685807656395887
12	35	-1.6955726735138485
12	36	-1.9272486772486772
12	37	-1.9913904450669149
12	38	-1.5422463429816373
12	39	-1.5594810146280729
12	40	-1.5915071584189224
13	1	6.044502801120451
13	2	12.976447245564891
13	3	14.310033457827572
13	4	-0.26248443821972955
13	5	10.614947089947089
13	6	19.170152505446627
13	7	19.970273109243696
13	8	20.181185807656398
13	9	19.947233893557424
13	10	19.294821817615937
13	11	18.987165421724242
13	12	21.138515406162465
13	13	19.128057889822596
13	14	21.022222222222222
13	15	15.062819016495485
13	16	24.279964207905387
13	17	24.880777310924366
13	18	25.04023887332711
13	19	26.933092125739186
13	20	26.094798474945534
13	21	27.156189698101464
13	22	24.299470899470897
13	23	27.060955493308438
13	24	27.07161920323685
13	25	28.456835511982568
13	26	14.124463118580767
13	27	14.69417211328976
13	28	14.614476346093992
13	29	13.311297852474322
13	30	15.448202614379085
13	31	12.208523965141612
13	32	-1.9059212573918458
13	33	-2.0861266728913783
13	34	-1.8685807656395887
13	35	-1.6955726735138485
13	36	-1.9272486772486772
13	37	-1.9913904450669149
13	38	-1.5422463429816373
13	39	-1.5594810146280729
13	40	-1.5915071584189224
15	1	-0.3126400560224063
15	2	-10.452124183006537
15	3	-7.118537970743857
15	4	-5.7624844382197296
15	5	-6.885052910052911
15	6	-6.472704637410516
15	7	-5.886869747899162
15	8	-4.390242763772173
15	9	-5.338480392156862
15	10	-2.419463896669777
15	11	-2.3699774354186154
15	12	-1.147198879551821
15	13	-3.2290849673202615
15	14	-6.12063492063492
15	15	-5.7943238406473725
15	16	-6.862892934951758
15	17	-6.5477941176470615
15	18	-5.245475412387177
15	19	-7.066907874260815
15	20	-7.905201525054466
15	21	-5.843810301898538
15	22	-6.200529100529103
15	23	-5.9390445066915625
15	24	-4.92838079676315
15	25	-5.04316448801743
15	26	-3.375536881419234
15	27	-2.80582788671024
15	28	-2.8855236539060076
15	29	-2.688702147525678
15	30	-2.051797385620916
15	31	-2.2914760348583885
15	32	-1.9059212573918458
15	33	-2.0861266728913783
15	34	-1.8685807656395887
15	35	-1.6955726735138485
15	36	-1.9272486772486772
15	37	-1.9913904450669149
15	38	-1.5422463429816373
15	39	-1.5594810146280729
15	40	-1.5915071584189224
16	1	-10.122163865546216
16	2	-14.023552754435109
16	3	-14.689966542172428
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107	19	7.183092125739185
107	20	2.0947984749455344
107	21	0.15618969810146233
107	22	6.299470899470897
107	23	3.5609554933084375
107	24	5.57161920323685
107	25	7.20683551198257
107	26	9.124463118580767
107	27	3.19417211328976
107	28	3.3644763460939924
107	29	0.561297852474322
107	30	2.948202614379084
107	31	4.7085239651416115
107	32	3.094078742608154
107	33	2.9138733271086217
107	34	5.131419234360411
107	35	5.8044273264861515
107	36	4.322751322751323
107	37	5.758609554933085
107	38	5.457753657018363
107	39	6.940518985371927
107	40	6.158492841581078
108	1	3.8778361344537835
108	2	-3.3568860877684426
108	3	-3.023299875505762
108	4	-0.0958177715530617
108	5	-1.5517195767195773
108	6	-3.3298474945533734
108	7	-0.029726890756304414
108	8	1.51451914098973
108	9	-3.0527661064425757
108	10	0.6281551509492704
108	11	7.487165421724242
108	12	4.138515406162465
108	13	4.294724556489264
108	14	5.022222222222222
108	15	5.729485683162153
108	16	5.946630874572053
108	17	-5.452556022408966
108	18	1.3735722066604428
108	19	3.2664254590725186
108	20	-7.905201525054466
108	21	-5.843810301898538
108	22	-6.200529100529103
108	23	-5.9390445066915625
108	24	-4.92838079676315
108	25	-5.04316448801743
108	26	-3.375536881419234
108	27	-2.80582788671024
108	28	-2.8855236539060076
108	29	-2.688702147525678
108	30	-2.051797385620916
108	31	-2.2914760348583885
108	32	-1.9059212573918458
108	33	-2.0861266728913783
108	34	-1.8685807656395887
108	35	-1.6955726735138485
108	36	-1.9272486772486772
108	37	-1.9913904450669149
108	38	-1.5422463429816373
108	39	-1.5594810146280729
108	40	-1.5915071584189224
109	1	6.794502801120451
109	2	10.976447245564891
109	3	10.310033457827572
109	4	12.73751556178027
109	5	7.864947089947089
109	6	12.420152505446627
109	7	13.220273109243696
109	8	11.431185807656398
109	9	13.697233893557424
109	10	-1.9551781823840635
109	11	4.237165421724242
109	12	4.138515406162465
109	13	6.378057889822596
109	14	3.772222222222222
109	15	0.31281901649548516
109	16	3.779964207905385
109	17	7.380777310924367
109	18	6.290238873327109
109	19	3.9330921257391847
109	20	7.844798474945534
109	21	-2.8438103018985377
109	22	2.7994708994708972
109	23	-0.4390445066915625
109	24	-1.6783807967631503
109	25	3.20683551198257
109	26	4.874463118580766
109	27	1.6941721132897598
109	28	7.614476346093992
109	29	10.561297852474322
109	30	0.4482026143790838
109	31	10.958523965141612
109	32	4.344078742608154
109	33	11.913873327108622
109	34	9.381419234360411
109	35	3.3044273264861515
109	36	3.572751322751323
109	37	12.008609554933084
109	38	4.957753657018363
109	39	2.440518985371927
109	40	4.908492841581078
110	1	-2.7054971988795486
110	2	-10.273552754435109
110	3	-9.689966542172428
110	4	-9.26248443821973
110	5	-8.885052910052911
110	6	-12.329847494553373
110	7	-13.029726890756304
110	8	-11.818814192343602
110	9	-12.052766106442576
110	10	-8.705178182384063
110	11	-12.512834578275758
110	12	-12.861484593837535
110	13	-13.371942110177404
110	14	-12.977777777777778
110	15	-12.937180983504515
110	16	-9.720035792094615
110	17	-9.119222689075633
110	18	-8.959761126672891
110	19	-7.066907874260815
110	20	-7.905201525054466
110	21	-5.843810301898538
110	22	-6.200529100529103
110	23	-5.9390445066915625
110	24	-4.92838079676315
110	25	-5.04316448801743
110	26	-3.375536881419234
110	27	-2.80582788671024
110	28	-2.8855236539060076
110	29	-2.688702147525678
110	30	-2.051797385620916
110	31	-2.2914760348583885
110	32	-1.9059212573918458
110	33	-2.0861266728913783
110	34	-1.8685807656395887
110	35	-1.6955726735138485
110	36	-1.9272486772486772
110	37	-1.9913904450669149
110	38	-1.5422463429816373
110	39	-1.5594810146280729
110	40	-1.5915071584189224
111	1	4.544502801120451
111	2	1.9764472455648914
111	3	13.310033457827572
111	4	11.23751556178027
111	5	-8.885052910052911
111	6	-12.329847494553373
111	7	-13.029726890756304
111	8	-11.818814192343602
111	9	-12.052766106442576
111	10	-8.705178182384063
111	11	-12.512834578275758
111	12	-12.861484593837535
111	13	-13.371942110177404
111	14	-12.977777777777778
111	15	-12.937180983504515
111	16	-9.720035792094615
111	17	-9.119222689075633
111	18	-8.959761126672891
111	19	-7.066907874260815
111	20	-7.905201525054466
111	21	-5.843810301898538
111	22	-6.200529100529103
111	23	-5.9390445066915625
111	24	-4.92838079676315
111	25	-5.04316448801743
111	26	-3.375536881419234
111	27	-2.80582788671024
111	28	-2.8855236539060076
111	29	-2.688702147525678
111	30	-2.051797385620916
111	31	-2.2914760348583885
111	32	-1.9059212573918458
111	33	-2.0861266728913783
111	34	-1.8685807656395887
111	35	-1.6955726735138485
111	36	-1.9272486772486772
111	37	-1.9913904450669149
111	38	-1.5422463429816373
111	39	-1.5594810146280729
111	40	-1.5915071584189224
112	1	-10.705497198879549
112	2	-10.523552754435109
112	3	-10.189966542172428
112	4	-14.01248443821973
112	5	-6.885052910052911
112	6	-8.579847494553373
112	7	-7.529726890756304
112	8	-8.818814192343602
112	9	-7.052766106442576
112	10	-6.4551781823840635
112	11	-6.262834578275758
112	12	-4.861484593837535
112	13	-5.371942110177404
112	14	-4.977777777777778
112	15	-4.937180983504515
112	16	-3.220035792094615
112	17	-2.6192226890756327
112	18	-4.209761126672891
112	19	-0.5669078742608153
112	20	-0.1552015250544656
112	21	-5.843810301898538
112	22	-6.200529100529103
112	23	-5.9390445066915625
112	24	-4.92838079676315
112	25	-5.04316448801743
112	26	-3.375536881419234
112	27	-2.80582788671024
112	28	-2.8855236539060076
112	29	-2.688702147525678
112	30	-2.051797385620916
112	31	-2.2914760348583885
112	32	-1.9059212573918458
112	33	-2.0861266728913783
112	34	-1.8685807656395887
112	35	-1.6955726735138485
112	36	-1.9272486772486772
112	37	-1.9913904450669149
112	38	-1.5422463429816373
112	39	-1.5594810146280729
112	40	-1.5915071584189224
113	1	-23.45549719887955
113	2	-14.023552754435109
113	3	-14.689966542172428
113	4	-15.76248443821973
113	5	-8.885052910052911
113	6	-12.329847494553373
113	7	-13.029726890756304
113	8	-11.818814192343602
113	9	-12.052766106442576
113	10	-8.705178182384063
113	11	-12.512834578275758
113	12	-12.861484593837535
113	13	-13.371942110177404
113	14	-12.977777777777778
113	15	-12.937180983504515
113	16	-9.720035792094615
113	17	-9.119222689075633
113	18	-8.959761126672891
113	19	-7.066907874260815
113	20	-7.905201525054466
113	21	-5.843810301898538
113	22	-6.200529100529103
113	23	-5.9390445066915625
113	24	-4.92838079676315
113	25	-5.04316448801743
113	26	-3.375536881419234
113	27	-2.80582788671024
113	28	-2.8855236539060076
113	29	-2.688702147525678
113	30	-2.051797385620916
113	31	-2.2914760348583885
113	32	-1.9059212573918458
113	33	-2.0861266728913783
113	34	-1.8685807656395887
113	35	-1.6955726735138485
113	36	-1.9272486772486772
113	37	-1.9913904450669149
113	38	-1.5422463429816373
113	39	-1.5594810146280729
113	40	-1.5915071584189224
114	1	5.794502801120451
114	2	6.976447245564891
114	3	13.560033457827572
114	4	12.98751556178027
114	5	4.114947089947089
114	6	7.670152505446627
114	7	7.970273109243696
114	8	-0.3188141923436021
114	9	10.197233893557424
114	10	7.5448218176159365
114	11	11.487165421724242
114	12	12.638515406162465
114	13	12.128057889822596
114	14	11.022222222222222
114	15	8.062819016495485
114	16	15.029964207905385
114	17	8.630777310924367
114	18	2.290238873327109
114	19	8.433092125739185
114	20	1.0947984749455344
114	21	13.656189698101462
114	22	10.549470899470897
114	23	13.810955493308438
114	24	9.82161920323685
114	25	8.95683551198257
114	26	16.624463118580767
114	27	5.94417211328976
114	28	8.114476346093992
114	29	4.561297852474322
114	30	-2.051797385620916
114	31	-2.2914760348583885
114	32	-1.9059212573918458
114	33	-2.0861266728913783
114	34	-1.8685807656395887
114	35	-1.6955726735138485
114	36	-1.9272486772486772
114	37	-1.9913904450669149
114	38	-1.5422463429816373
114	39	-1.5594810146280729
114	40	-1.5915071584189224
115	1	6.544502801120451
115	2	5.976447245564891
115	3	14.810033457827572
115	4	13.73751556178027
115	5	10.114947089947089
115	6	11.170152505446627
115	7	17.470273109243696
115	8	13.181185807656398
115	9	16.447233893557424
115	10	8.794821817615937
115	11	-4.012834578275758
115	12	5.138515406162465
115	13	9.128057889822596
115	14	15.022222222222222
115	15	5.062819016495485
115	16	3.779964207905385
115	17	5.380777310924367
115	18	-8.959761126672891
115	19	-7.066907874260815
115	20	-7.905201525054466
115	21	-5.843810301898538
115	22	-6.200529100529103
115	23	-5.9390445066915625
115	24	-4.92838079676315
115	25	-5.04316448801743
115	26	-3.375536881419234
115	27	-2.80582788671024
115	28	-2.8855236539060076
115	29	-2.688702147525678
115	30	-2.051797385620916
115	31	-2.2914760348583885
115	32	-1.9059212573918458
115	33	-2.0861266728913783
115	34	-1.8685807656395887
115	35	-1.6955726735138485
115	36	-1.9272486772486772
115	37	-1.9913904450669149
115	38	-1.5422463429816373
115	39	-1.5594810146280729
115	40	-1.5915071584189224
118	1	5.544502801120451
118	2	-4.023552754435109
118	3	12.310033457827572
118	4	12.23751556178027
118	5	15.114947089947089
118	6	6.670152505446627
118	7	10.970273109243696
118	8	3.181185807656398
118	9	9.947233893557424
118	10	3.2948218176159365
118	11	1.4871654217242423
118	12	10.138515406162465
118	13	4.628057889822596
118	14	9.022222222222222
118	15	16.062819016495485
118	16	-1.720035792094615
118	17	6.880777310924367
118	18	5.040238873327109
118	19	5.933092125739185
118	20	-5.905201525054466
118	21	20.156189698101464
118	22	17.799470899470897
118	23	22.060955493308438
118	24	5.07161920323685
118	25	16.956835511982568
118	26	23.624463118580767
118	27	-2.80582788671024
118	28	-2.8855236539060076
118	29	-2.688702147525678
118	30	-2.051797385620916
118	31	-2.2914760348583885
118	32	-1.9059212573918458
118	33	-2.0861266728913783
118	34	-1.8685807656395887
118	35	-1.6955726735138485
118	36	-1.9272486772486772
118	37	-1.9913904450669149
118	38	-1.5422463429816373
118	39	-1.5594810146280729
118	40	-1.5915071584189224
119	1	5.544502801120451
119	2	14.976447245564891
119	3	-7.689966542172428
119	4	-9.76248443821973
119	5	-2.885052910052911
119	6	-0.32984749455337337
119	7	2.9702731092436956
119	8	1.181185807656398
119	9	0.9472338935574243
119	10	7.2948218176159365
119	11	1.4871654217242423
119	12	1.1385154061624654
119	13	14.628057889822596
119	14	19.022222222222222
119	15	-12.937180983504515
119	16	-9.720035792094615
119	17	-9.119222689075633
119	18	-8.959761126672891
119	19	-7.066907874260815
119	20	-7.905201525054466
119	21	-5.843810301898538
119	22	-6.200529100529103
119	23	-5.9390445066915625
119	24	-4.92838079676315
119	25	-5.04316448801743
119	26	-3.375536881419234
119	27	-2.80582788671024
119	28	-2.8855236539060076
119	29	-2.688702147525678
119	30	-2.051797385620916
119	31	-2.2914760348583885
119	32	-1.9059212573918458
119	33	-2.0861266728913783
119	34	-1.8685807656395887
119	35	-1.6955726735138485
119	36	-1.9272486772486772
119	37	-1.9913904450669149
119	38	-1.5422463429816373
119	39	-1.5594810146280729
119	40	-1.5915071584189224
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
4	1074.0
5	46.0
6	80.0
7	39.0
8	40.0
9	68.0
10	154.0
11	137.0
12	124.0
13	162.0
14	160.0
15	146.0
16	124.0
17	114.0
18	109.0
19	79.0
20	96.0
21	80.0
22	62.0
23	96.0
24	62.0
25	55.0
26	41.0
27	33.0
28	25.0
29	25.0
30	6.0
31	8.0
32	3.0
33	2.0
34	0.0
35	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	0.0	0.0	100.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	0.0	0.0	100.0
5	0.0	100.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	98.70649830612874	0.8007391438250694	0.3695719125346474	0.12319063751154913
8	97.53770390889504	0.6771314250538628	0.8002462296091105	0.9849184364419821
9	96.24384236453201	1.416256157635468	1.1391625615763548	1.2007389162561575
10	48.98461538461538	28.4	11.138461538461538	11.476923076923077
11	32.97446939403261	27.8375884343279	18.824976930175332	20.362965241464163
12	36.9424792371578	13.16517994463242	24.76161181175023	25.13072900645955
13	26.33035988926484	13.103660412180867	25.192248538911105	35.373731159643185
14	20.270685942786834	20.33220547523839	34.543217471547216	24.85389111042756
15	27.653029836973236	18.394340203014455	29.960012303906492	23.992617656105814
16	32.912949861581055	18.91725622885266	24.823131344201784	23.346662565364504
17	29.19101814826207	23.254383266687174	25.68440479852353	21.870193786527224
18	28.914180252230082	20.39372500768994	28.66810212242387	22.023992617656106
19	26.52872143298332	16.646077825818406	18.37554045707227	38.449660284126004
20	28.637342356198094	26.637957551522607	22.116271916333435	22.608428175945864
21	27.74530913565057	19.870808981851738	29.59089510919717	22.792986773300523
22	21.16271916333436	18.055982774530914	28.514303291294986	32.266994770839744
23	26.115041525684408	19.74776991694863	27.16087357736081	26.97631498000615
24	25.71516456474931	21.193478929560136	28.3605044601661	24.730852045524454
25	28.35591133004926	19.642857142857142	22.598522167487683	29.40270935960591
26	20.846201358863496	18.746139592340953	27.733168622606545	32.67449042618901
27	18.528778085564788	16.62049861495845	29.085872576177284	35.76485072329948
28	17.53846153846154	21.53846153846154	28.584615384615386	32.33846153846154
29	26.27040344933785	17.400677548506312	27.96427471512165	28.364644287034185
30	20.720443349753694	15.548029556650247	34.79064039408867	28.94088669950739
31	22.674060382008626	18.66913123844732	32.84041897720271	25.816389402341343
32	26.892307692307693	13.446153846153846	32.15384615384615	27.507692307692306
33	22.369230769230768	15.015384615384617	33.6	29.015384615384615
34	17.62534604737004	17.410027683789604	39.03414334051061	25.930482928329745
35	15.230769230769232	16.553846153846155	39.23076923076923	28.984615384615388
36	15.476923076923077	21.26153846153846	35.44615384615384	27.815384615384612
37	17.159581022797287	15.588416512630932	37.89279112754159	29.35921133703019
38	18.123076923076923	15.692307692307692	32.738461538461536	33.44615384615385
39	19.6	17.2	32.46153846153847	30.738461538461536
40	16.70255306059674	15.933558904952323	36.48108274377115	30.882805290679794
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.5
15	1.0
16	2.0
17	3.0
18	3.0
19	9.0
20	15.0
21	19.0
22	23.0
23	23.0
24	22.5
25	22.0
26	29.5
27	37.0
28	37.0
29	36.5
30	36.0
31	43.0
32	50.0
33	50.0
34	59.5
35	69.0
36	89.0
37	109.0
38	109.0
39	111.5
40	114.0
41	120.5
42	127.0
43	127.0
44	139.0
45	151.0
46	153.0
47	155.0
48	155.0
49	159.0
50	163.0
51	186.0
52	209.0
53	209.0
54	219.5
55	230.0
56	238.0
57	246.0
58	246.0
59	251.0
60	256.0
61	296.0
62	336.0
63	336.0
64	325.5
65	315.0
66	278.0
67	241.0
68	241.0
69	196.0
70	151.0
71	127.5
72	104.0
73	104.0
74	73.0
75	42.0
76	34.0
77	26.0
78	26.0
79	19.5
80	13.0
81	8.5
82	4.0
83	4.0
84	3.0
85	2.0
86	1.0
87	0.0
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.12303906490310675
8	0.06151953245155337
9	0.09227929867733005
10	0.030759766225776686
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.3998769609350969
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.09227929867733005
26	0.3998769609350969
27	0.06151953245155337
28	0.030759766225776686
29	0.12303906490310675
30	0.09227929867733005
31	0.15379883112888343
32	0.030759766225776686
33	0.030759766225776686
34	0.0
35	0.030759766225776686
36	0.030759766225776686
37	0.15379883112888343
38	0.030759766225776686
39	0.030759766225776686
40	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
40	3251.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	94.80159950784373
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.72290720311486	91.69486311904029
2	2.336145360155743	4.429406336511843
3	0.5191434133679429	1.4764687788372808
4	0.16223231667748217	0.6151953245155337
5	0.0973393900064893	0.46139649338665023
6	0.03244646333549643	0.1845585973546601
7	0.0	0.0
8	0.06489292667099286	0.492156259612427
9	0.0	0.0
>10	0.06489292667099286	0.6459550907413103
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT	11	0.3383574284835435	No Hit
CTCCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC	10	0.30759766225776686	No Hit
CTCCATGGGTACCTGGTTGATCCTGCCCGTAGCATATGCT	8	0.2460781298062135	No Hit
CTCCATGGGGGGCTGGTGAGATGGCTCCGTGGGTAAGAGC	8	0.2460781298062135	No Hit
CTCCATGGGTACCTGGTTGATCCTGCCCGTAGCCTATGCT	6	0.1845585973546601	No Hit
CTCCATGGGTCGCAGAGTCGATTTTTTTTTTTTTTTTTTT	5	0.15379883112888343	No Hit
CTCCATGGGGGGGCTGGTGAGATGGCTCCGTGGGTAAGAG	5	0.15379883112888343	No Hit
CTCCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG	5	0.15379883112888343	No Hit
CTCCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCC	4	0.12303906490310675	No Hit
CTCCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG	4	0.12303906490310675	No Hit
CTCCATGGGGGGCTGGTGCGATGGCTCAGTGGGTAAGAGC	4	0.12303906490310675	No Hit
CTCCATGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT	4	0.12303906490310675	No Hit
CTCCATGGGATCCTGGGCCATTGGCCTCTCTGTGGCCGAC	4	0.12303906490310675	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.030759766225776683	0.0	0.0	0.0	0.0
12	0.030759766225776683	0.0	0.0	0.0	0.0
13	0.030759766225776683	0.0	0.0	0.0	0.0
14	0.030759766225776683	0.0	0.0	0.0	0.0
15	0.030759766225776683	0.0	0.0	0.0	0.0
16	0.030759766225776683	0.0	0.0	0.0	0.0
17	0.030759766225776683	0.0	0.0	0.0	0.0
18	0.030759766225776683	0.0	0.0	0.0	0.0
19	0.030759766225776683	0.0	0.0	0.0	0.0
20	0.030759766225776683	0.0	0.0	0.0	0.0
21	0.030759766225776683	0.0	0.0	0.0	0.0
22	0.030759766225776683	0.0	0.0	0.0	0.0
23	0.030759766225776683	0.0	0.0	0.0	0.0
24	0.030759766225776683	0.0	0.0	0.0	0.0
25	0.030759766225776683	0.0	0.0	0.0	0.0
26	0.030759766225776683	0.0	0.0	0.0	0.0
27	0.030759766225776683	0.0	0.0	0.0	0.0
28	0.030759766225776683	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGGGGT	25	1.618756E-4	34.0	5
ATGGGGG	80	0.0	34.0	5
ATGGGGC	20	0.002401324	34.0	5
ATGGGGA	30	1.0804797E-5	34.0	5
TGGGGGG	55	1.2732926E-11	34.0	6
CATGGGT	25	1.618756E-4	34.0	4
CATGGGG	155	0.0	34.0	4
CTCCATG	330	0.0	32.969696	1
TCCATGG	320	0.0	32.9375	2
CCATGGG	300	0.0	32.866665	3
CATGGGA	70	0.0	31.571428	4
CATGGGC	50	8.414645E-9	30.599998	4
ATGGGAT	35	3.1064832E-5	29.142857	5
ATGGGCT	25	0.0071645197	27.199999	5
GGGGGGG	45	5.088983E-6	26.444443	7
>>END_MODULE